Winston Lab Strain Finder
Department of Genetics

Bacteria


Displaying 2001 - 2500 of 2807
Contains
Strain Name Strain Alias Source Comments Host Host Markers Plasmids Plasmid Markers
FB2115 pJM189 J. MADison human yeast chimera DH5a...... pJM238 "p425MET25,Sc194-337,human1-179
FB2116 pJM203 J Madison human yeast SPT3 chimera DH5a...... pJM203 "p425MET25,Sc306-337, human 1-289
FB2117 pJM204 J Madison human yeast SPT3 chimera DH5a...... pJM204 "p425MET25, SPT3 Sc1-24 human 43-317aa's
FB2118 pJM205 J Madison yeast human chimera DH5a...... pJM205 "p425MET25,SPT3 Sc1-61 human 80-317aa's
FB2119 pJM206 J. Madison human yeast SPT3 chimera in p425MET25 DH5a...... pJM206 "LEU2,2micron, Sc1-103aas human 113-317
FB2120 pJM195 J. Madison human yeast SPT3 chimera in p425MET25 DH5a...... pJM195 "LEU2,2micron,Sc 1-194 aas human 182-317 aas
FB2121 pJM29 J. Madison human yeast SPT3 chimera in p425MET25 DH5a...... pJM209 "LEU2,2micron,Sc64-337 aa's human 1-79 aa's
FB2122 pJM1 J. Madison R1 fragment of Clarispora opuntiae in pRS426 DH5a...... pJM1 "2micron,URA3,CoSPT3
FB2123 pJM2 J.Madison Xho/H3 subclone of FB2122 in pRS426 DH5a...... pJM2 "2micron,URA3,CoSPT3
FB2124 pJM3 J.Madison Kluyueromyces lactis R1 fragment in pRS426 comp. spt3D203 DH5a...... pJM3 "2micron,URA3,KlSPT3
FB2125 pJM4 J.Madison subclone of FB2125 in pRS426 DH5a...... pJM4 "2micron,URA3,KlSPT3
FB2126 pJM14 J.Madison Spx1.1 Spombe genomic clone from REP genomic library HB101..... pJM14 S.pombe vector
FB2127 pJM15 J.Madison in pRS426 HB101..... pJM15 "2micron,URA3, Spombe SPT3
FB2128 h/hp77B1 J.Madison DH5a...... h/hp77B1 "URA3,CEN, wt hTBP in pRS316
FB2129 p77B1 J.Madison DH5a...... p77B1 "URA3,CEN,mutant TBP in pRS316
FB2130 JMB76 L.Guarent SpTBP in pRS426 Nature 346:291-294 DH5a...... "2micron,URA3 SpTBP
FB2131 pJM64 J.Madison BstX1 site destroyed DH5a...... pJM64 "pRS316 URA3, CEN,
FB2132 pJM81 J.Madison HB101..... pJM81 "pRS316,URA3,CEN,mot1-1 gap rescue cand.
FB2133 pJM90 J.Madison HB101..... pJM90 "pRS314,CEN,TRP1,MOT1+
FB2134 pJM111 J.Madison BL21/DE3.. pJM111 T7prom. His6-HA-SPT3
FB2135 pJM116 J.Madison DH5a.DE3.. pJM116 pGEX T7prom GST-SPT3
FB2136 pJM118 J.Madison DH5a.DE3.. pJM118 "pRS426,URA3,2micron,TOA2
FB2137 pJM120 J.Madison DH5a.DE3.. pJM120 "pRS426,URA3,2micron,TOA1
FB2138 pJM122 J.Madison Use FB2484 - pLQ99 instead of pJM122 DH5a.DE3.. pJM122 "pRS426,TOA1,TOA2,2micron,URA3
FB2139 pJM126 J.Madison DH5a.DE3.. pJM126 "2micron,URA3,GALprom-GST-SPT3
FB2140 pJM165 J.Madison DH5a.DE3.. pJM165 "LEU2,2micron,SPT3 prom HIS6-SPT3
FB2141 p49 M.Snyder DH5a.DE3.. p49 KNS1
FB2142 p54 M.Snyder DH5a.DE3.. p54 KNS1
FB2143 p55 M.Snyder DH5a.DE3.. p55 "KNS1,p55
FB2144 JMB210 L.Stargell/Struhl Science 269:75-78 DH5a.DE3.. SPT15 modified
FB2145 JMB211 L.Stargell/Struhl Science 269: 75-78 DH5a.DE3.. TOA1-SPT15
FB2146 JMB212 L. Stargell Science 269:75-78 DH5a...... TOA2-SPT15
FB2147 p39 M.Snyder DH5a...... p39 KNS1
FB2148 pRS416-0 David Stillman NH975 pRS416-0 "Ycp, URA3
FB2149 pJM183 J.Madison DH5a...... pJM183 "p425MET25, HA-ScSPT31-337
FB2150 pJM185 J.Madison DH5a...... pJM185 "p425MET25, Ha1-SpombeSPT3
FB2151 pJM186 J.Madison DH5a...... pJM186 "p425MET25, HA1-K.lactis SPT3
FB2152 pJM182 J.Madison BL21 pJM182 6HisNterm-MOT3 pet vector
FB2153 pJM189 JMadison DH5a...... pJM189 "YEp13, MOT3
FB2154 pJM224 J.Madison DH5a...... pJM224 "HA-Human SPT3, pBabe pwo
FB2155 pJM223 J.Madison made from pDE119 DH5a...... pJM223 Cterm 6His-HA-SPT3
FB2156 pJM188 JMadison DH5a...... pJM188 MOT3, CEN, LEU2 (pRS315)
FB2157 pJM190 JMadison DH5a...... pJM190 "CKS1, CEN, URA3 (pRS316)
FB2158 pJM225 JMadison DH5a...... pJM225 "pBSK+, Glu-Glu tagged MOT3
FB2159 pJM227 JMadison DH5a...... pJM227 "CEN, LEU2, Glu-Glu tagged MOT3 (pRS315)
FB2160 pJM228 JMadison DH5a...... pJM228 "CEN, LEU2, Glu-Glu tagged MOT3
FB2161 pJM229 JMadison DH5a...... pJM229 "pRS425, 2micron, LEU2, MOT3
FB2162 pJM231 JMadison DH5a...... pJM231 "pRS425, LEU2, 2micron, Glu-Glu-MOT3
FB2163 pJM186 JMadison DH5a...... pJM186 "pBSK+, MOT3
FB2164 pJM191 JMadison DH5a...... pJM191 "CKS1, pRS316, URA3 CEN
FB2165 pJM220 JMadison DH5a...... pJM220 "pRS306, URA3, Yip, PTA1
FB2166 pJM187 J.Madison DH5a...... pJM187 "p425MET25, MOT3
FB2167 pJM198 JMadison DH5a...... pJM198 "p425MET25, HA1-ScSPT3 1-315aa
FB2168 pJM184 JMadison DH5a...... pJM184 "p425MET25, HA1-C.OpmtialSPT3
FB2169 pJM235 JMadison DH5a...... pJM235 "pRS306, URA3, Yip, toa1-31
FB2170 pJM233 JMadison DH5a...... pJM233 "pJG4-6, pGAL-HA-1-hTAFii18
FB2171 pJM232 JMadison DH5a...... pJM232 "p425MET25, Glu-Glu-MOT3
FB2172 pJM234 JMadison DH5a...... pJM234 "pRS306, URA3, Yip, toa2-9
FB2173 pMLTATA S. Buratowski "Nature 334,37 1988 " DH5a...... pMLTATA "pSP65, wild type TATA element
FB2176 p64 pYK116 Giora Simchen DH5a...... p64 pYK116 "yEp24, CDC40
FB2177 pWS3202-1 Jim Haber DH5a...... pWS3202-1 "rad3-1::TRP1, yCp50
FB2178 pSH325 John Kang DH5a...... pSH325 "TOA1, pRS316
FB2179 pSH342 John Kang DH5a...... pSH342 "TOA2, pRS316
FB2180 pJYC1513 Arno Greenleaf unknown... pJYC1513 "CTK1, pRS316
FB2174 pMLTATAm1 SBuratowski DH5a...... pMLTATA-mut1 pSP65 vector mut1-TTATCAT
FB2175 pMLTATAm-2 S.Buratowski DH5a...... pMLTATA-mut2 pSP65-vector mut2-TAGAGAA
FB2181 David Stillman "resistant to killing by DNA gyrase, for use with pRS416-0 " NH975.....
FB2182 Beckwith Lab DHB4......
FB2183 pJT10 David Amberg based on YEplac195 ?.B4...... pJT10 "ADE8, URA3
FB2184 pGH202 Grant Hartzog DH5a...... pGH202 "URA3, CEN, rpb1-244
FB2185 pGH203 Grant Hartzog DH5a...... pGH203 "URA3, CEN, rpb1-221
FB2186 pHL11 G. Fink "cut with BglII to integrate, See notebook for ref " DH5a...... pHL11 "FLO8, URA3, Yip pRS316
FB2187 pDM1 Delia O'Rourke DH5a...... pDM1 HHT2-HHF2/URA3/CEN/ARS
FB2188 pDM9 Delia O'Rourke DH5a...... pDM9 HHT1-HHF1/URA3/CEN/ARS
FB2189 pDM10 Delia O'Rourke DH5a...... pDM10 HHT1-HHF1/TRP1/CEN/ARS
FB2190 PDM12 DELIA O'ROURKE DH5A...... pDM12 HHT2-HHF2/TRP1/CEN/ARS
FB2191 pDM14 DELIA O'ROURKE DH5A...... pDM14 HHT2-HHF2/TRP1/CEN/ARS
FB2192 pDM16 DELIA O'ROURKE DH5a...... pDM16 HHT1-HHF1/TRP1/CEN/ARS
FB2193 pDM18 DELIA O'ROURKE DH5a...... pDM18 HHT2-HHF2/TRP1/CEN/ARS
FB2194 pDB20LADA1 David McNabb Guarente lab DH5a...... pDB20LADA1 "Padh, ADA1, LEU2, 2micron
FB2195 pHZ1 Heng Zhou DH5a...... pHZ1 "SUC2, URA3, CEN
FB2196 pRSETb Grace Gill DH5a...... pRSETb
FB2197 pRSETc Grace Gill DH5a...... pRSETc
FB2198 pGEX4T-1 Grace Gill from Pharmacia DH5a...... pGEX4T-1
FB2199 pGEX4T-3 Grace Gill from Pharmacia DH5a...... pGEX4T-3
FB2200 pGEX5X-1 Grace Gill from Pharmacia DH5a...... pGEX5X-1
FB2201 pRSETa GRace Gill DH5a...... pRSETa
FB2202 p101 BCairns DH5a...... p101 "URA3,CEN,SWP61
FB2203 P102 B Cairns DH5a...... p102 URA3 CEN SWP59
FB2204 pBL50 B Laurent via B Cairns DH5a...... pBL50 URA3 2micron STH1
FB2205 pS340 B Cairns NPL6 aka RSC7 DH5a...... pS340 Ycp50 URA3 CEN NPL6
FB2206 p138 b cairns dh5a...... p138 "URA3,CEN,RSC6
FB2207 p147 b cairns DH5a...... p147 "URA3,CEN,TFG3
FB2208 p149 b cairns DH5a...... p149 URA3 CEN SWP73
FB2209 p151 b cairns TAF130 and RSC14 separated by only 80bp DH5a...... p151 "URA3,2micron,RSC14 w/TAF130
FB2210 p164 b cairns DH5a...... p164 "URA3,CEN,RSC4
FB2211 p166 b cairns DH5a...... p166 "URA3,CEN,RSC1
FB2212 p178 b cairns DH5a...... p178 URA3 CEN RSC3
FB2213 p197 b cairns DH5a...... p197 TRP1 CEN RSC1
FB2214 pRSQ2-URA3 Snyder lab for use with the Tn3-LEU2-LacZ library ?.5a...... pRSQ2-URA3
FB2218 pJM200 J Madison DH5a...... pJM200 pRS306 SUPT3H
FB2215 pLP4 L Pacella integrating ADA1 plasmid under ADH1 prom and term DH5a...... pLP4 "URA3,promADH1 -ADA1-tADH1
FB2216 PHJM1 B OLSEN ?.5a...... PHJM1 "pBluescript, 5' end of SUPT3H cDNA
FB2217 p123089 B Olsen pBluescript derivative ?.5a...... p123089 Est clone p123089 into pT7T30
FB2219 pAD19 A Dudley see notebook for specific mutation MH1....... pAD19 "Ty912D44-lacZ (#19), TRP1
FB2220 pAD20 A. Dudley see notebook for specific mutation MH1....... pAD20 "TRP1, Ty912D44-lacZ(#20)
FB2221 pAD22 A Dudley see notebook for specific mutation MH1....... pAD22 "TRP1, Ty912D44-lacZ(#22)
FB2222 pAD23 A Dudley see notebook for specific mutation MH1....... pAD23 "TRP1, Ty912D44-lacZ(#23A)
FB2223 pAD24 A Dudley see notebook for specific mutation MH1....... pAD24 "TRP1, Ty912D44-lacZ(#24)
FB2224 pAD27 A Dudley see notebook for specific mutation MH1....... pAD27 "TRP1, Ty912D44-lacZ(clean #18)
FB2225 pAD28 A Dudley see notebook for specific mutation MH1....... pAD28 "TRP1, Ty912D44-lacZ(#12/13)
FB2226 pAD29 A Dudley see notebook for specific mutation MH1....... pAD29 "TRP1, Ty912D44-lacZ(#17/18)
FB2227 pAD30 A Dudley see notebook for specific mutation MH1....... pAD30 "TRP1, Ty912D44-lacZ(#13/18)
FB2228 pAD31 A Dudley see notebook for specific mutation MH1....... pAD31 "TRP1, Ty912D44-lacZ(#23B)
FB2229 pAD25 A Dudley see notebook for specific mutation MH1....... pAD25 "URA3, Ty912D44-lacZ(#25)
FB2230 pJM199 J Madison HB101..... pJM199 "pRS306, 3' end of SUPT3H
FB2231 RY2973 Nancy Woychik ?.101..... RY2973 "URA3, SRB10, CEN
FB2232 YCplac22 Gietz lab DH5a...... YCplac22 "TRP1, CEN4, LacZ
FB2233 YCplac33 Gietz lab DH5a...... YCplac33 "URA3, CEN4, LacZ
FB2234 YCplac111 Gietz lab DH5a...... YCplac111 "LEU2, CEN4, LacZ
FB2235 YEplac195 Gietz lab DH5a...... YEplac195 "URA3, 2micron, LacZ
FB2236 YEplac181 Gietz lab DH5a...... YEplac181 "LEU2, 2micron, LacZ
FB2237 YIplac204 Gietz lab DH5a...... YIplac204 "TRP1, LacZ
FB2238 YIplac211 Gietz lab DH5a...... YIplac211 "URA3, LacZ
FB2239 YIplac128 Gietz lab DH5a...... YIplac128 "LacZ, LEU2
FB2241 YCP50-TRT2 "M.A, OSLEY DH5A...... YCp50-TRT2 "URA3,CEN,TRT2
FB2242 pRP82 N Woyzhick ?.5A...... pRP82 "RPB8, pBSK2(-)
FB2243 pIP45 Ines Pinto.on SpeI/HindIII frag used for loading control on Northerns DH5a pIP45 "SPT15, pBluescript KS+
FB2244 pGN1622 Jeff Boeke ?......... pGN1622 "Spt10-C387S, LEU2, pRS304
FB2245 pGN961 Jeff Boeke ?......... pGN961 "SPT21, CEN, LEU2, YCp50
FB2246 BM3076 Mark Johnston DH5a...... ' BM3076 "CEN, URA3, 3XMyc::MIG1
FB2247 pJM142 Jon Madison MH1....... pJM142 "LEU2, MOT3, 2micron
FB2248 pCH2 Cintia Hongay MH1....... pCH2 "URA3, MOT3, CEN
FB2249 p5921 William Fonzi for making C.albicans deletions DH5a...... p5921 "pUC18, hisG-URA3-hisG
FB2250 pGEMT-HIS1 Aaron Mitchell for making deletions by PCR DH5a...... pGEMT-HIS1 C.albicans HIS1
FB2251 pGEMT-URA3 Aaron Mitchell for making deletions by PCR DH5a...... pGEMT-URA3 C.albicans URA3
FB2252 pRS-ARG4 Aaron Mitchell for making deletions by PCR DH5a...... pRS-ARG4 DSpeI C.albicans ARG4
FB2253 Gal1-FLP1 Snyder lab to cure endogenous 2micron from yeast strain ?......... YEp351-Gal-FLP1 "2micron, LEU2, Gal1p-FLP1
FB2254 pSE1111 C. Brandl MH1....... pSE1111 "2micron, LEU2, SNF4
FB2255 pSE1112 C. Brandl MH1....... pSE1112 "2micron, TRP1, SNF1
FB2256 pAS1 C. Brandl 2-hybrid MH1....... pAS1 "TRP1, 2micron
FB2257 pACTII C Brandl 2 hybrid MH1....... pACTII "TRP1, 2 micron
FB2258 pEV229 Eric Verdin vector from Promega HB101..... pEV229 "HIV-LTR, pGL2-Basic
FB2259 pEV374 Eric Verdin same as pEV229 except HIV enhancer replaced by 5 Gal4 binding sites HB101..... pEV374 "5 Gal4 b.s., HIV-LTR, pGL2-Basic
FB2260 pEV280 Eric Verdin HB101..... pEV280 "Tat-2-exon-FLAG, pCDNA3.1
FB2261 pEV279 Eric Verdin HB101..... pEV279 "Tat-1-exon-FLAG, pCDNA3.1
FB2262 pRS-SWI2 S.Biggar "ref Peterson NAR 1996, V.24 No.19 3685-3692 " DH5a...... pRS-SWI2 "CEN, LEU2, SWI2
FB2263 pRS6-3 S. Biggar Embo V18 no8 2254-2264 DH5a...... pRS6-3 "CEN, LEU2, Swi2 mts in ATPase domain T909A, P943L, E1077G
FB2264 pHZ5 Heng Zhou DH5a...... pHZ5 "CEN, URA3
FB2265 pHZ52 Heng Zhou MH1....... pHZ52 "NRG1, URA3, 2micron
FB2266 Erp50L1 Heng Zhou DH5a...... Erp50L1 "Ura3, 2micron, Nrg1
FB2267 pEV541 Eric Verdin see notebook for additional info DH5a...... pEV541 pFLAG-CMV2-hCDK9 in pLV1392
FB2268 pEV542 Eric Verdin see notebook for more info DH5a...... pEV542 pCMV-HA-CyclinT in PGCN
FB2269 pYSL12 Anne Dranginis MCB V9 1/98 161-171 DH5a...... pYSL12 "pRS425, LEU2, 2micron, FLO11
FB2270 pYSL13 Anne Dranginis MCB V9 1/98 161-171 DH5a...... pYSL13 "pRS426, URA3, 2micron, FLO11
FB2271 pRJS1 Rebecca Spencer "mutations in ATPase domain T909A, P943L, E1077G " DH5a...... pRJS1 "pRS406, URA3 Snf2-TS
FB2272 SB905 Steve Buratowski S28....... pRS314-FLAG-CCL1 "TFA1 pr-CCL1 w/N terminal FLAG, TRP1, CEN/ARS
FB2273 pDM397 David McNabb CYC1 probe DH5a...... pDM397 pGEMT CYC1
FB2274 pYGFP3 Brendan Cormack "Cormack et. al., (1997) Microbiology, 143:303-311 " DH5a...... pYGFP3 "S65G, S72A mutations in GFP, super GFP
FB2275 pUC13D16' Alex Bortvin DH5a...... pUC13D16' URA3 with flanking BamH1 sites
FB2276 pLY50 Brehon Laurent "(1990) MCB 10: 5616-5625, pLY50 is same as pLY14 " DH5a...... pLY50 "4.8kb R1-BamH1 frag. from SNF5, pRS316
FB2277 pLY17 Brehon Laurent (1990) MCB 10: 5616-5625 DH5a...... pLY17 "yIP5, snf5D2
FB2278 snf5-51ts Brehon Laurent single point mutation: E582K DH5a...... snf5-51ts "snf5-51ts, pRS306
FB2279 snf5-83b Brehon Laurent single point mutation D475N DH5a...... snf5-83b "snf5-83b, pRS306
FB2280 pMC134 Karen Arndt DH5a...... pMC134 "tup1D::HIS3, pUC118
FB2281 pBM2887 Mark Johnston "see notebook, made by Karl-Dieter Entian " DH5a...... pBM2887 "hxk2D::LEU2,
FB2282 pPS1 or M6 Priya Sudarsanam DH5a...... pPS1orM6 "URA3, suc2 with URA3 inserted in it, 2micron
FB2283 pPS4 Priya Sudarsanam DH5a...... pPS4 "pBKSII, Super GFP with SUC2 flanking region
FB2284 p1229 Chris Brandl vector pT27 DH5a...... p1229 tra1::hisG-URA3-hisG
FB2285 GC41 Bik Tye HB101..... GC41 "MCM1, Bluescript KS-
FB2286 pGY107 Jan Fassler ?.101..... pGY107 "CEN,URA3,MYC-MCM1
FB2287 p-GCN5 for making recombinant GCN5 for HAT assays BL21(DE3). pRSetB-GCN5 GCN5
FB2288 pRS317 Joe Martens MH1....... pRS317 "LYS2, CEN, ARS
FB2289 C.alb-SPT3 Lisa Pacella original C.albicans genomic clone DH5a...... Yep352-SPT3 "URA3, 2micron, C.albicans SPT3
FB2290 pLP305 Mary Bryk DH5a...... pLP305 "SIR4,2micron, LEU2
FB2291 pRS4.2 Danesh Moazed see notebook for ref. DH5a...... pRS4.2 sir4D::HIS3
FB2292 RC4 Jef Boeke Cup1 promoter plasmid DH5a...... RC4 "Cup1 prom, galK, Cyc1 term, pBR322
FB2293 Danesh Moazed DH5a...... pRS315-SIR3 "CEN, LEU2, SIR3
FB2294 pJR104 Danesh Moazed yEp24 based plasmid DH5a...... pJR104 "2micron,URA3,SIR3
FB2295 MBB81 Mary Bryk see notebook for map and sequence DH5a...... MBB81 "sir3D::hisGURA3hisG, pBST KS
FB2296 pCK22 Craig Kaplan Spt5cDNA clone from BDGP via Genome Systems ?......... pCK22 "pBSIIK+ LD10265, Amp
FB2297 pCK151 Craig Kaplan Spt4cDNA BDGP via Research Genetics ?......... chloramphenicol pCK151 pOT2a LD44495 Spt4cDNA
FB2298 pIP49 Ines Pinto pRS425 DH5a...... pIP49 "ACT3,2micron,LEU2
FB2299 pWZV87 Nasmyth Lab DH5a...... pWZV87 Myc9-KlTRP1 in pUC19
FB2300 pWZV88 Nasmyth Lab DH5a...... pWZV88 Myc18-Kl-TRP1 in pUC19
FB2301 pGN1101 Boeke Lab SpeI/HindIII SPT10 genomic fragment in pRS415 epicurean. ampR pGN1101 "CEN, LEU2, SPT10
FB2302 pBAD18 Beckwith lab for expressing protein in bacteria see notebook DH5a.rean. pBAD18 "pBR322 Ori, Ara-inducible
FB2303 pBAD24 Beckwith Lab for expressing protein in bacteria see notebook DH5a.rean. pBAD24 pBR322 based ori Ara-inducible
FB2304 pBAD33 Beckwith Lab for expressing protein in bacteria see notebook DH5a.rean. CM res pBAD33 "pACYC origin, Ara-inducible
FB2305 pBS1539 Seraphin Lab "DO NOT GIVE OUT"" see notebook for more info """ MC1066.... AmpR pBS1539 "URA3-Kl, TAP tag
FB2306 pBS1479 Seraphin Lab "DO NOT GIVE OUT"" see notebook for more info """ MC1066.... AmpR pBS1479 "TRP1-Kl, TAP tag
FB2307 pJAM63 Joe Martens MH1.66.... AmpR pJAM63 "2micron,TRP1,ADH1prom,hCDK9,ADH1term
FB2308 pJAM64 Joe Martens MH1.66.... AmpR pJAM64 "CEN, TRP1, ADH1prom, hCDK9, ADH1 term
FB2309 pJAM65 Joe Martens MH1.66.... AmpR pJAM65 "2micron, HIS3, ADH1prom, hCYCLIN-T1, ADH1term
FB2310 pJAM66 Joe Martens MH1.66.... AmpR pJAM66 "CEN, HIS3, ADH1prom, hCYCLIN-T1, ADH1term
FB2338 CKB134 Craig Kaplan "pRS414 backbone, K1274Stop mutation " DH5a...... pCK134 "spt6-50FLAG, TRP1, CEN, ARS
FB2311 kanMX6 Mark Longtine see notebook for more info. DH5a...... pFA6a-kanMX6 "kanMX6,
FB2312 pFA6a-TRP1 Mark Longtine see notebook for more information DH5a...... pFA6a-TRP1 TRP1
FB2313 His3MX6 Mark Longtine see notebook for more information DH5a...... pFA6a-His3MX6 His3MX6
FB2314 GFP-kanMX6 Mark Longtine see notebook for more information DH5a...... pFA6a-GFP-kanMX6 "GFP(S65T),ADH1term,kanMX6
FB2315 GFP-TRP1 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6A-GFP-TRP1 "GFP(S65T),ADH1term,TRP1
FB2316 GFPHIS3MX6 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-GFP-HIS3MX6 "GFP(S65T),ADH1term,HIS3MX6
FB2317 3HA-kanMX6 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-3HA-kanMX6 "3HA, ADH1term, kanMX6
FB2318 3HA-TRP1 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-3HA-TRP1 "3HA,ADH1term, TRP1
FB2319 3HA-HIS3 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-3HA-His3MX6 "3HA, ADH1term, His3MX6
FB2320 13Myc-kan Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-13Myc-kanMX6 "13Myc, ADH1term, kanMX6
FB2321 13Myc-TRP1 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-13Myc-TRP1 "13Myc, ADH1term, TRP1
FB2322 13Myc-His3 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-13Myc-His3MX6 "13Myc, ADH1term, His3MX6
FB2323 GST-kanMX6 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-GST-kanMX6 "GST, ADH1term, kanMX6
FB2324 GST-TRP1 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-GST-TRP1 "GST, ADH1term, TRP1
FB2325 GST-His3 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-GST-His3MX6 "GST, ADH1term, His3MX6
FB2326 kan-PGAL1 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-kanMX6-PGAL1 "kanMX6, Gal1 promoter
FB2327 TRP1-PGAL1 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-TRP1-PGAL1 "TRP1, GAL1 promoter
FB2328 His3-PGAL1 Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-His3MX6-PGAL1 "His3MX6, GAL1 promoter
FB2329 kanGalHA Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998)"

pFA6a-kanMX6-PGAL1-3HA 		DH5a...... pFA6a-KanMX-3HA "kanMX6, GAl1 prom, 3HA, ADH1term
FB2330 TRP1GALHA Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998)"

pFA6a-TRP1-PGAL1-3HA 		DH5a...... pFA6a-TRP1-PGAL1-3HA "TRP1, GAL1prom, 3HA, ADH1term
FB2701 Mei Sheng/Niroshi Senaratne Generated by Mei Sheng during her rotation by inserting URA3 into LacZ in the pRS313 Myc-SPN1 HIS3 CEN AmpR plasmid received by Erin Loeliger from the Stargell Lab. This plasmid containing URA3 was extracted from yeast by Niroshi Senaratne and transformed into E. coli, here stored as FB2701. DH5a HIS3 URA3 Myc-SPN1 AmpR CEN
FB2331 His3GALHA Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... pFA6a-HIS3-PGAL1-3HA "His3MX6, GAL1prom, 3HA, ADH1term
FB2332 KanGAlGST Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... kanMX6-PGAL1-GST "kanMX6, GAL1prom, GST, ADH1term
FB2333 TRp1GALGST Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... TRP1-PGAL1-GST "TRP1, GAL1prom, GST, ADH1term
FB2334 His3GALGST Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... His3MX6-PGAL1-GST "His3MX6, GAL1prom, GST, ADH1term
FB2335 kanGALGFP Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... kanMX6-PGAL1-GFP "kanMX6, GAL1prom, GFP(S65T), ADH1term
FB2336 TRP1GALGFP Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... TRP1-PGAL1-GFP "TRP1, GAL1prom, GFP(S65T), ADH1term
FB2337 His3GALGFP Mark Longtine "see notebook for more information, Yeast 14, 953-961 (1998) " DH5a...... His3MX6-PGAL1-GFP "His3MX6, GAL1prom, GFP(S65T), ADH1term
FB2339 RC4-2 Lisa Pacella made from FB2292 DH5a...... RC4-2 "pBR322, CUP1promoter, CYC1terminator
FB2340 p25 Alan Goldstein "nourseothricin resistance, Yeast 15, 1541-1553 (1999) " DH5a...... p25 "natMX4, pFA6 vector backbone
FB2341 p29 Alan Goldstein "Bialaphos resistance, Yeast 15, 1541-1553 (1999) " DH5a...... p29 "patMX4, pFA6 vector backbone
FB2342 p32 Alan Goldstein "Hygromycin B resistance, Yeast 15, 1541-1553 (1999) " DH5a...... p32 "hphMX4, pFA6 vector backbone
FB2343 pPS48 Karen Arndt "pRS316, ref. Genetics 152:73 1999 " HB101..... pPS48 "SNF4, URA3, CEN
FB2344 CKB34 Craig Kaplan BglII-PvuII DmSPT6 from LD17780 MH1....... pCK34 "GST-DmSPT6, pGEX1
FB2345 CKB35 Craig Kaplan BglII-EcoR1 frag from LD17780 MH1....... pCK35 "GST-DmSPT6, pGEX1
FB2346 CKB36 Craig Kaplan BglII-BglII frag from LD10265 DH5a...... pCK36 "GST-DmSPT5, pGEX4T-1
FB2347 CKB37 Craig Kaplan BglII-BamHI fragment from LD10265 DH5a...... pCK37 "GST-DmSPT5, pGEX4T1
FB2348 CKB38 Craig Kaplan EcoR1-PvuII fragment from LD02327 DH5a...... pCK38 "GST-DmSPT6, pGEX5X-1
FB2349 CKB43 Craig Kaplan BglII-XhoI fragment from LD10265 DH5a...... pCK43 "GST-DmSPT5, pGEX5X-1
FB2350 CKB44 Craig Kaplan BglII-XhoI fragment from LD10265 DH5a...... pCK44 "GST-DmSPT5, pGEX5X-1
FB2351 CyclinT David Price via Craig for affinity purifying Cyclin T antibodies BL21...... pCK150 "T7-GST-DmCyclinT, pET vector
FB2352 pLexA-SNF2 Brehon Laurent XL1 Blue.. pLexA-SNF2 "2micron, HIS3, ADH1p-LexA-SNF2
FB2353 ADH1p-SNF2 Brehon Laurent XL1 Blue.. ADH1p-SNF2 "2micron, LEU2, ADH1p-SNF2
FB2354 pNKY349 Schiestl lab "ref Alani et.al, Cell 1990 " DH5a...... pNKY349 "rad51-K181, URA3
FB2355 pLP15 Lisa Laprade DH5a...... pLP15 "TRP1, CEN3, CUP1prom, FLO11, CYC1term
FB2356 Natalie Kuldell XL1....... pRS415-TFG2 "LEU2, CEN, TFG2
FB2357 Natalie XL1....... pRS416-TFG2 "URA3, CEN, TFG2
FB2358 M1.1 Natalie K DH5a...... pRS415-tfg2M1.1 "LEU2, CEN, tfg2-M1.1
FB2359 M2.4 Natalie K DH5a...... pRS415-tfg2M2.4 "LEU2, CEN, tfg2-M2.4
FB2360 c3.10 Natalie K DH5a...... pRS415-tfg2c3.10 "LEU2, CEN, tfg2-c3.10
FB2361 c4.5 Natalie K DH5a...... pRS415-tfg2c4.5 "LEU2, CEN, tfg2-c4.5
FB2362 pAFS59 Aaron Straight ref Current Biology 6:1599-1608 1996 Stable2... pAFS59 "lac operator repeat, YIPlac128-LEU2
FB2363 pAFS135 Aaron Straight ref Current Biology 6:1599-1608 1996 Stable2... pAFS135 "HIS3p-GFP12-LacI-I12, pRS303
FB2364 pAFS144 Aaron Straight ref Current Biology 6:1599-1608 1996 Stable2... pAFS144 HIS3p-GFP13-LacI-I12
FB2365 pAFS91 Aaron Straight ref Current Biology 6:1599-1608 1996 DH5a.e2... pAFS91 HIS3p-GFP(s65t)-TUB1
FB2366 pAFS52 Aaron Straight ref Current Biology 6:1599-1608 1996 Stable2... pAFS52 lacO YIPlac204
FB2367 pAFS114 Aaron Straight ref Current Biology 6:1599-1608 1996 DH5a.e2... pAFS114 TEL4 lacO LEU2
FB2368 pGP256 Greg Prelich DH5a...... pGP256 "BUR6, URA3, YCP50
FB2369 pGP258 Greg Prelich DH5a...... pGP258 "BUR6, CEN, URA3
FB2370 pGP273 Greg Prelich DH5a...... pGP273 "bur6D::TRP1, CEN, URA3
FB2371 pGP412 Greg Prelich DH5a...... pGP412 "YDR1, CEN, URA3
FB2372 pl200 David Pellman Ref: Current Biology 8:1211-1214 DH5a...... pl200 "PKC1, URA3, 2micron
FB2373 pSH24 David Pellman Ref. Genetics 149:99 unknown... pSH24 "PKC1, URA3, 2micron
FB2374 pRC81 Cintia Hongay DH5a...... pRC81 URA3 CEN PAN1
FB2375 pHC6 Cintia Hongay DH5a...... pHC6 LEU2 CEN VPS41
FB2376 pCH11 Cintia Hongay pRS315 DH5a...... pCH11 LEU2 CEN VPS41
FB2377 pCH12 Cintia Hongay pRS305 DH5a...... pCH12 LEU2 VPS41
FB2378 pJR2330 Jasper Rine DH5a...... pJR2330 LEU2 2micron ECM22
FB2379 pJR2341 Jasper Rine DH5a...... pJR2341 URA3 2micron VPC2
FB2380 pCH7 Cintia Hongay pRS416 DH5a...... pCH7 URA3 CEN PAN1
FB2381 pCH9 Cintia Hongay pRS305 DH5a...... pCH9 LEU2 PAN1
FB2382 JK0024 Jerry Kaplan DH5a...... JK0024 URA3 2micron VPS41
FB2383 pAS249 Alan Sachs YCp50 DH5a...... pAS249 URA3 CEN PAN1
FB2384 pJAM198 Joe Martens DH5a...... pJAM198 kanMX 2micron
FB2385 pJAM200 Joe Martens DH5a...... pJAM200 kanMX 2micron LEXA-SNF2
FB2386 pLBS41 R. Sternglanz DH5a...... pLBS41 "pet28, SPT10-HIS, SPT21, Kan
FB2387 pLBS42 R. Sternglanz DH5a...... pLBS42 "pet28a, His-SPT10(aa15-641) SPT21 Kan
FB2388 pCWD29 R. Sternglanz DH5a...... pCWD29 "pet28a, HIS-SPT10(aa15-641), Kan
FB2389 pLBS27 R. Sternglanz DH5a...... pLBS27 pet28b SPT10-HIS kan
FB2390 pLBS39 R. Sternglanz DH5a...... pLBS39 "pet17b, SPT21, Amp
FB2391 M598 David Stillman DH5a...... M598 "HO, URA3, CEN, YCp50
FB2392 M1063 David Stillman DH5a...... M1063 "HO, HIS3, pBR322
FB2393 pLP1347 Etik Spedale DH5a...... pLP1347 TRP1
FB2394 pLP1348 Erik Spedale DH5a...... pLP1348 "TRP1, ESA1
FB2395 pLP1349 Erik Spedale DH5a...... pLP1349 "TRP1, esa1-E388Q dom-
FB2396 pRET3a-H2A Danesh Moazed DH5a...... pRET3a-H2A H2A
FB2397 pRET3a-H2B Danesh Moazed DH5a...... pRET3a-H2B H2B
FB2398 pRET3a-H3 Danesh Moazed DH5a...... pRET3a-H3 H3
FB2399 pRET3a-H4 Danesh Moazed DH5a...... pRET3a-H4 H4
FB2400 pPC97 Steve Buratowski DH5a...... pPC97 "ADH1p, Gal4 binding domain, LEU2, CEN
FB2401 pLS21-5 Karen Arndt pRS314 based ?......... pLS21-5 HA-RTF1 CEN TRP1
FB2402 pCK210 Craig Kaplan pRS306 DH5a...... pCK210 URA3 flo8D5'
FB2403 pCK211 Craig Kaplan pRS306 deletion of internal promoter DH5a...... pCK211 "URA3 flo8D5',DTATA+1626
FB2404 pCK212 Craig Kaplan pRS306 GAL10-7 integrating DH5a...... pCK212 GAL10-7 URA3
FB2405 pCK213 Craig Kaplan +1002 from GAL10 ATG to +974 from GAL7 ATG DH5a...... pCK213 GAL10-7 URA3
FB2406 pCK214 Craig Kaplan FLO8 TATA at +1626 deleted pRS306 DH5a...... pCK214 flo8DTATA+1626 URA3
FB2407 pCK215 Craig Kaplan 2nd isolate of internal TATA deleted DH5a...... pCK215 flo8DTATA+1626 URA3
FB2408 pCK216 Craig Kaplan pRS306 based DH5a...... pCK216 gal10D55b gal7DTATA URA3
FB2409 pCK217 Craig Kaplan 2nd isolate in pRS306 DH5a...... pCK217 gal10D55b gal7DTATA URA3
FB2410 pJW10 Jenny Wu DH5a...... pJW10 pRS406 Spt7-D3373-3453
FB2412 pKD1 Krista Dobi pRS416 backbone DH5a...... pKD1 URA3 CEN GAL4
FB2411 pJW11 Jenny Wu pRS406 based DH5a...... pJW11 "spt7Dnt 3451-3543, URA3
FB2413 pJW12 Jenny Wu DH5a...... pJW12 spt7Dnt3373-3543 URA3
FB2414 p75 Nick Proudfoot ref EMBO V.17 No.16 p.4771-4779 DH5a...... p75 GAL10 3'UTR deletion CEN TRP1
FB2415 pNOY436 Nomura Lab ref PNAS v.94 pp.13458-13462 1997 DH5a...... pNOY436 "HHT2-mycHHF2, TRP1, CEN
FB2416 RY2522 Rick Young DH5a...... pRP1-1 "rpb1-1, URA3, CEN
FB2417 pT7-IID Steve Buratowski DH5a...... pT7-IID T7prom SPT15
FB2418 pRS414-H4 Craig Peterson Nature Structural Biology V9 p167 2002 MH1 pRS414-H4 "TRP1, CEN, HHF2
FB2419 414-h4R45C Craig Peterson Nature Stuctural Biology V.9 p.167 2002 MH1....... pRS414-hhf2-R45C "TRP1, CEN, hhf2-R45C
FB2420 pAAD3 Andrea Duina DH5a...... pAAD3 "hht2-3-HHF2 TRP1, CEN
FB2421 pAAD5 Andrea Duina DH5a...... pAAD5 hht2-5-HHF2 TRP1 CEN
FB2422 pAAD7 Andrea Duina DH5a...... pAAD7 hht2-7-HHF2 TRP1 CEN
FB2423 pAAD11 Andrea Duina DH5a...... pAAD11 hht2-11-HHF2 TRP1 CEN
FB2424 pAAD41 Andrea Duina DH5a...... pAAD41 hht2-41-HHF2 TRP1 CEN
FB2425 pAAD44 Andrea Duina DH5a...... pAAD44 hht2-44-HHF2 TRP1 CEN
FB2426 pAAD48 Andrea Duina DH5a...... pAAD48 hht2-48-HHF2 TRP1 CEN
FB2427 pAAD49 Andrea Duina DH5a...... pAAD49 hht2-48-HHF2 TRP1 CEN
FB2428 pAAD51 Andrea Duina DH5a...... pAAD51 hht2-51-HHF2 TRP1 CEN
FB2429 pAAD56 Andrea Duina DH5a pAAD56 hht2-56-HHF2 TRP1 CEN
FB2430 pAAD57 Andrea Duina DH5a...... pAAD57 hht2-57-HHF2 TRP1 CEN
FB2431 pAAD180 Andrea Duina MH1....... pAAD180 hht2-11-HHF2 TRP1 2micron
FB2432 pPM263 Pam Meluh TOP10..... pPM263 URA3 CEN HTA1 Myc-HTB1
FB2433 pDH66 Nadia Roan/David Hess DH5a...... pDH66 spt10-11 LEU2 CEN
FB2434 pDH21 David Hess DH5a...... pDH21 LexA-spt10-199 TRP1 ADE2 2micron
FB2435 pGCYC1-402 A. Aguilera DH5a...... pGCYC1-402 pGAL1-double G-less cassette URA3 CEN
FB2436 p416GAL1 A. Aguilera DH5a...... p416-GAL1-lacZ pGAL1-lacZ URA3 CEN
FB2437 pSch227 A. Aguilera DH5a...... Sch227 "pGAL1-LYS2, URA3 CEN
FB2438 pSch247 A. Aguilera DH5a...... pSch247 pGAL1-YAT1 URA3 CEN
FB2439 pSch202 A. Aguilera DH5a...... pSch202 pGAL1-PHO5 URA3 CEN
FB2440 pCM184-LAu A. Aguilera DH5a...... pCM184-LAUR pTet-lacZ-URA3
FB2441 pSch204 A. Aguilera DH5a...... pSch204 leu2-lacZ-leu2 TRP1 CEN
FB2442 SGP4 M. Green DH5a...... SGP4 GAL4 BSx3 pRS416
FB2443 L90 Doug Koshland "18S, 5.8S and part of 25S BglII fragment see notebook " HB101..... p362 rDNA FISH probe
FB2444 L92 Doug Koshland "25S and part of 5S BglII fragment, see notebook " HB101..... p363 rDNA fish probe
FB2445 pGH262 Grant Hartzog DH5a...... pGH262 SPT24 URA3 CEN
FB2446 pLL65 Amine Lefebvre and Smith 1993 DH5a...... pLL65 LEU2 SPT2-359 2micron
FB2447 pLL10 Amine Lefebvre and Smith 1993 DH5a...... pLL10 LEU2 2micron SPT2
FB2448 pLL18 Amine Lefebvre and Smith 1993 DH5a...... pLL18 LEU2 2micron SPT2-1
FB1151 pPT2 Patrick Tan ***tagged SPT7 in dam- strain*** see also FB1148 MM572..... dam- See FB274 pPT2 SPT7-HAI LEU2 CEN ARS ampR
FB1152 pCC58 Chris Clark 4.6kb SPT16 BamHI frag cloned into BamHI site of YCp50 (orientation A) HB101..... pCC58 SPT16 URA3 ampR tetS
FB1153 pCC61 Chris Clark 5.7kb SalI frag containing HTA1-HTB1 cloned into SalI site of YEp24. HB101..... pCC61 HTA1-HTB1 URA3 ampR 2micron
FB1154 pCC64 Chris Clark ~6.7kb HindIII frag containing HHT1-HHF1 cloned into HindIII site of pCGS42. HB101..... pCC64 HHT1-HHF1 2micron URA3 ampR
FB1155 pCC66 Chris Clark 2.6kb HindIII frag w/ HHT2 + 1.48 HindIII frag w/ HHF2 both cloned into Hind III site of pCGS42. HB101..... pCC66 HHT2-HHF2 URA3 2micron ampR tetR
FB1156 pCC67 Chris Clark 6.4kb HTA1-HTB1 Bam frag + 5.4kb HHT1-HHF1 Bam-H3 frag were cloned into the H3 and Bam sites of pCGS42. HB101..... pCC67 HHT1-HHF1 HTA1-HTB1 2micron URA3 ampR
FB1157 pDE92-4 D. Eisenmann = high copy spt15-21 with SalI site HB101..... pDE92-4 spt15-21 2micron TRP1 ampR
FB1158 pDE93-6 D. Eisenmann #NAME? HB101..... pDE93-6 SPT15 2micron TRP1 ampR
FB1159 pDE105-6 D. Eisenmann **used for gapped rescue of SPT3 alleles by cutting with MluI and XhoI** HB101..... pDE105-6 SPT3 URA3 CEN ARS ampR
FB1160 pDE118-4 D. Eisenmann SPT3 cloned into M13 + mut to insert an NdeI site at the ATG. This frag (RI -H3) was cloned into pRS426. **has SPT3+ activity** HB101..... pDE118-4 SPT3 with NdeI site at ATG 2micron URA3 ampR
FB1161 pDE119-1 D. Eisenmann SPT3 mut in M13 to attach 9 a.a. Lerner epitope at SPT3 N terminal. An RI-H 3 frag containing this was cloned into pRS426. **has SPT3+ activity + epit ope tag IN VIVO** HB101..... pDE119-1 SPT3 with Lerner epitope at NT URA3 2micron ampR
FB1162 pDE120-4 D. Eisenmann see FB1161 *** Lerner tagged SPT3 C terminal*** has SPT3+ activity but tag is not seen by Western HB101..... pDE120-4 SPT3 with Lerner epitope at CT 2micron URA3 ampR
FB1163 pDE133-12 D. Eisenmann ***Lerner tagged spt3-401*** HB101..... pDE133-12 spt3-401 with Lerner epitope at NT 2micron URA3 ampR
FB1848 pIL35 E. Dubois see reference in book ?......... pIL35 "ampR, URA3, CEN4/ARS1, Ty Region A, TY2 PvuII-Sau3, TDH3 DUAS-la cZ
FB1849 pIL37 E. Dubois see reference in book ?......... pIL37 "ampR, URA3, CEN4/ARS1, Ty Region A- TDH3 DUAS-lacZ, Ty2=PAE1-6, PvuII-Sau3
FB1850 pIL40 E. Dubois see reference in book ?......... pIL40 "ampR, URA3, CEN4/ARS1, Ty REgion-A, PvuII-Sau3 Ty1 +PAE2-6, TDH3 DUAS-lacZ
FB1851 pcI-13 E. Dubois see reference in book ?......... pcI-13 "ampR, tec1::URA3
FB1852 p6/50 Stan Field via Elaine Elion ?......... P6/50 "ampR, URA3, CEN, STE12
FB1853 pNKY1009 N. Kleckner lab ref. Genetics 116:541-545(1987) DH5a...... pNKY1009 "ampR, Trp1::hisG-URA3-hisG, ARS1,
FB1854 MPY3xHA Brandt Schneider vector for HA tagging your favorite gene DH5a...... MPY3xHA "URA3, PUCori, ampR
FB1855 MPY3XMYC Brant Schneider Vector for MYC tagging your favorite gene DH5a...... MPY3xMYC "ampR, URA3, PUCori
xit
FB2449 pRS406-709 Erica Larschan DH5a pRS406-7099 srb10-3 URA3
FB2450 pRS406-GAL Erica Larschan DH5a pRS406-GAL80 GAL80 URA3
FB2451 A1-1 Erica Larschan Rose Library Clone DH5a A1-1 SRB9-YCP50 URA3 CEN SRB9
FB2452 A2-2 Erica Larschan Rose Library Clone DH5a A2-2 SRB9 Ycp50 SRB9 URA3 CEN
rothstein
pGH127
pGH%
FB2453 yCp50pHIR1 MaryAnn Osley DH5a yCp50-HIR1 HIR1 URA3 CEN
FB2454 yCp50HIR2 Mary Ann Osley DH5a yCp50-HIR2 HIR2 URA3 CEN
FB2455 pGP106 Greg Prelich DH5a pGP106 BUR2 URA3 CEN
FB2456 pET-15b Amine DH5a pET-15b
FB2457 pIC208s Jacques Cote DH5a pIC208s 5s rDNA for nucleosome assembly template
FB
FB2463 pRS317 Natalie Kuldell DH5a pRS317 LYS2 CEN
FB2462 pRS307 Natalie Kuldell DH5a pRS307 LYS2 integrating
FB2461 4N Bob Kingston for assembling a tetra-nucleosome array ? 4N
FB2460 3N Bob Kingston for assembling a tri-nucleosome array ? 3N
FB2459 2N Bob Kingston for assembling a di-nucleosome array ? 2N
FB2458 1N Bob Kingston For assembling a mono-nucleosome ? 1N
FB2458 pBKS-1N Kingston Lab 5S rRNA nucleosomal positioning sequence
for mono-nucleosome assembly 		pBKS-1N 5S rRNA
FB2459 pBKS-2N Kingston Lab 5S rRNA nucleosomal positioning sequence
for di-nucleosome assembly 		pBKS-2N 5S rRNA
FB2460 pBKS-3N Kingston Lab 5S rRNA nucleosomal positioning sequence
for tri-nucleosome assembly 		pBKS-3N 5S rRNA
FB2461 pBKS-4N Kingston Lab 5S rRNA nucleosomal positioning sequence
for tetra-nucleosome assembly 		pBKS-4N 5S rRNA
FB2462 pRS307 Natalie Kuldell LYS2 pRS integrating plasmid DH5a pRS307 LYS2
FB2463 pRS317 Natalie Kuldell LYS2 CEN plasmid DH5a pRS317 LYS2, CEN
FB2464 pRS327 Natalie Kuldell LYS2 2 micron plasmid DH5a pRS327 LYS2 2micron
pFW217
FB2465 pDH17 Dave Hess spt10-G199A,G201A DH5a pDH17 spt10-199 LEU2 CEN
FB2466 pDH62 Dave Hess spt10-D122A. May not Transform.

Transformation attempted by 2 people, twice each, and failed all times. Sequencing by Lisa Laprade confirmed spt10-122 mutation. Michael Grunstein's lab claims there are a number of mutations in addition to spt10-122. 		DH5a pDH62 spt10-122 LEU2 CEN
FB2467 pDH63 Dave Hess spt10-G199A,G201A DH5a pDH63 spt10-199 LEU2 2micron
FB2468 pDH64 Dave Hess spt10-D122A DH5a pDH64 spt10-122 LEU2 2micron
FB2469 pDH65 Dave Hess & Nadia Roan DH5a pDH65 SPT10 LEU2 2micron
pRM200
pRM%
ura3 his3
snf1
ura3
FB2470 pDP2 Don Prather pRS426 + ELF1 DH5a pDP2 ELF1 2micron URA3
FB2471 pKR41 Mary Ann Osley Ubiquitin DH5a pKR41 GAL1-Ha-Ubiquitin
FB2472 pDP1 Don Prather pRS416 + ELF1 DH5a pDP1 ELF1 CEN URA3
FB2473 RY2210 Rick Young rpb1D110 - 13 3/7 repeats of CTD DH5a pV5 rpb1D110 LEU2 CEN
FB2474 RY2201 Rick Young rpb1D101 11 2/7 repeats of CTD DH5a pC1 rpb1D101 LEU2 CEN
FB2475 GHB190 Grant Hartzog DH5a pGH215 kin28-16 LEU2 CEN
FB2476 GHB200 Grant Hartzog rpb1D103 - 10 3/7 repeats of CTD DH5a pC3 rpb1D103 LEU2 CEN
FB2477 GHB232 Jeff Corden via G. Hartzog DH5a pY1wt(14) rpb1-CTD-D-HA CEN LEU2
FB2478 GHB233 Jeff Corden via G. Hartzog DH5a pYIwt(7)A5(7) rpb1-CTD-D-HA1 LEU2 CEN
FB2479 GHB234 Jeff Corden via G. Hartzog DH5a pY1wt(9)A2(6) rpb1-CTD-D-HA1 LEU2 CEN
FB2480 pCM224 Enrique Herrero dh5a pCM224 KanMx4-tTA-tetO2
%FLAG%
FB2483 pLQ3 Karen Arndt TFIIA HB101 pLQ3 TOA1 TOA2 LEU2 2micron
FB2481 pLQ1 Karen Arndt HB101 pLQ1 TOA1 LEU2 2micron
FB2482 pLQ2 Karen Arndt HB101 pLQ2 TOA2 LEU2 2micron
FB2484 pLQ99 Karen Arndt TFIIA DH5a pLQ99 TOA1 TOA2 URA3 2micron
FB2485 YCF3 Hieter lab CHL1 in acentric vector DH5a pYCF3 CHL1 URA3 ARS3 SUP11 TEL cassette
FB2486 pYCF4 Hieter lab CHL1 in centric vector pYCF4 DH5a pYCF4 CHL1 ARS3 SUP11 CEN4 TEL cassette
FB2487 pESC-URA3 Grunstein Lab XL1 Blue pESC-URA3 URA3 2micron
FB2488 pFX12 Grunstein Lab XL1 Blue pFX12 SPT10-13xMYC 2micron URA3
FB2489 pFX16 Grunstein lab XL1 Blue pFX16 spt10-122-13xMyc URA3 2micron
FB2490 pFX17 Grunstein Lab XL1 Blue pFX17 spt10-199-13xMyc URA3 2micron
Joel
hml
%hml%
FB2491 pGN965 pGN965
FB2491 pGN965 Jeff Boeke Ref: The New Biologist V.3 No.12 p1249-1259 (1991)
YEp13 		pGN965 ura3-153 LEU2 2micron
FB2492 BL21/DE3 Rolf Sternglanz Super codon plus strain for protein expression BL21/DE3 Chloramphenicol
FB2493 pET28B Rolf Sternglanz for protein expression Novagen pET28B Kanamycin
FB2494 pPS47 Kren Arndt pRS316 vector with SNF4-204 allele HB101 pPS47 SNF4-204 URA3 CEN
pNOY436
FB2495 pET28b-H2A Carl Wu Supercodon plus strain expressing H2A1 BL21 pET28B-H2A1 H2A1
FB2496 Carl Wu H2B1 in supercodon plus strain BL21 pET28b-H2B1 H2B.1
FB2497 Carl Wu H3.2 supercodon plus strain BL21 pET28b-H3.2 H3.2
FB2498 Carl Wu H4.2 supercodon plus strain BL21 pET28b-H4.2 H4.2
FB2499 CKB25 Craig Kaplan SPT6 with amino terminal FLAG tag cloned into YCp50 DH5a pCK25 SPT6-FLAG URA3 CEN
FB2500 CKB40 Craig Kaplan amino terminal FLAG tagged SPT6 in pRS306 DH5a pCK40 SPT6-FLAG URA3
FB2501 CKB125 Craig Kaplan amino terminal FLAG tagged spt6-1004 in pRS306 DH5a pCK25 spt6-1004-FLAG URA3
fb2195
phz1
FB2502 3xHA-TBP Kevin Struhl DH5a 3xHA-TBP 3xHA-TBP URA3 CEN
pms4
pms4
FB2503 pDM776 D. Moazed DH5a pFA6a-kanMX6-p3nmt1 kanMX6 nmt1 promoter
FB2504 pDM777 D. Moazed DH5a pFA6a-kanMX6-P41nmt1 KanMx6 attenuated nmt1 promoter
FB2505 pDM778 D. Moazed DH5a pFA6a-kanMX6-P81nmt1 kanMX6 attenuated nmt1 promoter
FB2506 pDM745 D. Moazed DH5a pFA6akanMX6P3nmt13HA kanMX6 3HA nmt1 promoter
FB2507 pDM746 D. Moazed DH5a pFA6akanMX6P41nmt13H kanMX6 P41nmt1 3HA
FB2508 pDM747 D. Moazed DH5a pFA6akanMX6P81nmt13H kanMX6 P81nmt1 3HA
FB2509 pKG1991 D. Moazed DH5a pREP1-NT LEU2 Pnmt1 Tnmt1
FB2510 pKG1992 D. Moazed same as FB2509 except extended polylinker/multiple cloning site DH5a pREP41-NT LEU2 Pnmt1 Tnmt1
FB2511 pKG1993 D. Moazed DH5a pREP81-NT pREP81-Pnmt1 Tnmt1 LEU2
FB2512 pKS-ura4 DH5a DH5a p358 pBluescript Sp. ura4
FB2516
FB2513 p1810 K.Gould DH5a pFA6a-kanMX6-CTAP2 kanMX6 2xproteinA TAP tag
FB2514 p2021 K. Gould DH5a pFA6a-KanMX6-CTAP4 kanMX6 4xproteinA TAP tag
FB2515 p1810-NAT Amine DH5a p1810-NAT replace kanR with NatR
FB2516 p2845 N. Rhind p2021 / BamH1-Pac1 DH5a pFS209 3xHA 5' TAP tag
FB2517 p30412 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-3HA-natMX6 3xHA natMX6
FB2518 p30413 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-13Myc-natMX6 13xMyc natMX6
FB2519 p30414 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-GST-natMX6 GST nat-MX6
FB2520 p30415 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-C-TAP4-natMX6 C-TAP4 natMX6
FB2521 p30416 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-GFP(S65T)-natM GFP(S65T) nat-MX6
FB2522 p30417 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-ECFP-natMX6 ECFP natMX6
FB2523 p30418 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-EYFP-natMX6 EYFP natMX6
FB2524 p30419 Euroscarf Yeast 2005;22:1061-1068 DH5a yEGFP3-CLN2PEST-nat yEGFP3-CLN2PEST-natMX6
FB2525 p30420 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-pADH1-3HA natMX6 pADH1-3HA
FB2526 p30421 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-natMX6-pGAL1 pGAL1 natMX6
FB2527 p30422 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-pGAL1-3HA natMX6 pGAL1-3HA
FB2528 p30423 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-pGAL1-GST natMX6 pGAL1-GST
FB2529 p30424 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-pGAL1-GFP natMX6 pGAL1-GFP
FB2530 p30425 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-natMX6-P3nmt1 P3nmt1 natMx6
FB2531 p30426 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-natMX6-P41nmt1 P41nmt1 natMX6
FB2532 p30427 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-natMX6-P81nmt1 P81nmt1 natMX6
FB2533 p30428 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-P3nmt1-3HA P3nmt1-3HA natMx6
FB2534 p30429 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-P41nmt1-3H natMX6 P41nmt1 3HA
FB2535 p30430 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-P81nmt1-3H natMX6 P81nmt1 3HA
FB2536 P30431 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-P3nmt1-GST natMX6 P3nmt1 GST
FB2537 p30432 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-P41nmt1GST natMX6 P41nmt1 GST
FB2538 p30433 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-P81nmt1GST natMX6 P81nmt1 GST
FB2539 p30434 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-P3nmt1-GFP natMX6 P3nmt1 GFP
FB2540 p30435 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-P41nmt1GFP natMX6 P41nmt1 GFP
FB2541 p30436 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-nat-P81nmt1GFP natMX6 P81nmt1 GFP
FB2542 p30437 Euroscarf Yeast 2005;22:1013-1019 DH5a pFA6a-natMX6 natMX6
FB2543 p30438 Euroscarf Yeast 2005;22:1013-1019 DH5a pFA6a-hphMx6 hphMX6
FB2544 p30439 Euroscarf Yeast 2005;22:1013-1019 DH5a pFA6a-bleMX6 bleMX6
FB2545 pDM291 David McNabb Biotechniques 22:1134-1139 (1997) DH5a pDM291 hisG URA4+ (S.pombe) hisG
FB2792 CKB30 Craig Kaplan pRS414 backbone DH5a CKB30 TRP1 CEN ARS ampR SPT6-FLAG
FB2546 pDK-414 Charlie Hoffman TOP10F' pDK-414 S. pomber ARS1 ura4+
FB2547 ZM467 Zarmik from Struhl lab DH5a ZM467 3xFLAG URA3
FB2548 pMBV40 Jim Haber DH5a pMBV40 HO::HygB
FB2549 pDL1 Diana Libuda DH5a pDL1 coq1 pRS303
FB2550 pDL2 Diana Libuda pRS414 DH5a pDL2 HTA1-HTB1 TRP1 CEN
FB2551 pAR K.Takegawa Yeast 2005;22:193-202 DH5a pAR arg12+ pombe ARS
FB2552 pAY K. Takegawa Yeast 2005;22:193-202 DH5a pAY tyr1+ pombe ARS
FB2553 pAAD K.Takegawa Yeast 2005;22:193-202 Dh5a pAAD ade7+ pombe ARS
FB2554 pAH K.Takegawa Yeast 2005;22:193-202 DH5a pAH his2+ pombe ARS
FB2555 pARTR K.Takegawa Yeast 2005;22:193-202 DH5a pARTR arg12+ pombe ARS
FB2556 pARTY K.Takegawa Yeast 2005;22:193-202 DH5a pARTY tyr1+ pombe ARS
FB2557 pARTAD K.Takegawa Yeast 2005;22:193-202 DH5a pARTAD ade7+ pombe ARS
FB2557 pARTH K.Takegawa Yeast 2005;22:193-202 DH5a pARTH his2+ pombe ARS
FB2559 pHY68 Hideki Yashiroda Top10 pHY68 3xFLAG CYC1 terminator KanMx6

Copyright © The President and Fellows of Harvard College