Winston Lab Strain Finder
Department of Genetics

Bacteria


Displaying 1001 - 1500 of 2807
Contains
Strain Name Strain Alias Source Comments Host Host Markers Plasmids Plasmid Markers
FB881 Dave Sal site put in by Karen; see notebook TB1....... pDE73-21 pRS316 SPT15 with Sal site at 18 bp upstream of ATG
FB882 Dave TB1....... pDE74-6 pRS316 spt15-26
FB883 Dave see notebook; Sal site 18 bp upstream of ATG TB1....... pDE75-2 pRS314 SPT15-Sal
FB884 Dave Sal site 18 bp upstream of ATG TB1....... pDE76-4 pCGS42 SPT15-Sal
FB885 F.W. Studier FB885-894 are for expression via T7 RNA polymerse. See Fred or Greg for the article describing all of these vectors and strains. BL21 (DE3) pLysS
FB886 F.W. Studier see FB885 HB21 (DE3) pLysE
FB887 F.W. Studier see FB885 BL21.(DE3) none
FB888 F.W. Studier see FB885 BL21 (DE3) none
FB889 F.W. Studier see FB885 HMS174.E3) none
FB890 F.W. Studier see FB885 HMS174.E3) pET3
FB891 F.W. Studier see FB885 HMS174.E3) pET-39
FB892 F.W. Studier see FB885 HMS174.E3) pET-36
FB893 F.W. Studier see FB885 HMS174.E3) pET-8c
FB894 F.W. Studier see FB885 HMS174.E3) pET-3c
FB895 Bob Moreland "see Cell 45, 537-544 " HB101..E3) pSKAdel8-7 HTA1-lacZ fusion
FB896 Kate see notebook TB1.1..E3) pFW253 HTB2-lacZ fusion
FB897 Kate TB1.1..E3) pFW254 pRS314 SPT7 Bam-RI
FB898 Joel Bases 1-228 by Genbank TB1.1..E3) pJH11 pUC18 HIS4 Sal-Xho 228 bp fragment
FB899 Joel Opposite orientation as in FB898 TB1.1..E3) pJH12 pUC18 HIS4 Sal-Xho 228 bp fragment
FB900 Dave for rescue of spt15 alleles; cut with RI; see FB877 for TRP1 version HB101..... pDE38-9 pRS316 SPT15 6.6 kb Cla-Sac
FB901 Kate TB1....... pFW255 pRS314 SPT7 Cla-RI
FB902 Christy "probe for HTA1, HTB1; also includes protein 1 " HB101..... pCC68 pBR322 HTA1-HTB1 5.5kb NheI-SalI
FB903 Kate HB101..... pFW256 YCp50 HTA2-HTB2
FB904 Kate HB101..... pFW257 pCGS42 HTA2-HTB2
FB905 Suzanne Sandmeyer "Ty3 probe; MCB 8, 5245 " HB101..... pTy3-2 "amp, F1 ori, pBR322 ori, T7 promoter, Ty3-2
FB906 Maynard Olsen "Gene 27, 239 " unknown... pPM256 "2 micron, LEU2, SUP53-a
FB907 Maynard Olsen note: SUP4-a Bam fragment is 235 bp. See Molecular Cell Biology 4 (1984) p. 657-665 unknown... pPM609 SUP4-a TRP1 ARS1 CEN3 ampR
fb908 M.A. Osley .97 kb H3 promoter of TRT2 cloned into YIp5 HB101.n... Yip5-2.97M TRT2 promoter URA3 ampR
FB909 Michele Swanson/ Fred Winston 1.5kb Bam-Sal fragment from pAD5 cloned into Bam-Sal sites of 8.0kb YEp353 TB1 ..n... pMS35 ampR ura3 2micron
FB910 Tricia Davis .B1 ..n...
FB911 Tricia Davis AB1886 F- uvrA6 thr1 leu6 thi1 lacV1 galkz ara14 xyl5
FB912 Tricia Davis SEE FB911 and enclosed information in notebook AB1886.... pGW249 pKM10bla::Tri5 kanR
Fb913 Michele Swanson/ Fred Winston =6kb BglII fragment enclosing SPT6 at BamHI site of YCp50(pCC11) with insert ion of HAI epitope sequences between first and second codon of SPT6 HB101..... pMS33 ampR URA3 CEN ARS SPT6
FB914 "K.Ferguson.n, Wigler lab see notebook HB101 pAD5 Lerner Epitope
FB915 Christine Chapon SPT8 Bam-Bam in YCp50; HAS NO FUNCTION unknown... pY2 SPT8
FB916 Christine Chapon SPT8 Bam-Bam in YCp50; HAS NO FUNCTION; is a duplicate of FB915 unknown... pY3 SPT8
FB917 Christine Chapon SPT8 Bam-Bam in YCp50; opposite orientation as FB916; has partial function unknown... pY7 SPT8
FB918 Christine Chapon SPT8 Bam-Bam in YCp50; opposite orientation as FB916; has partial function; is a duplicate of pY7 in FB917 unknown... pY8 SPT8
FB919 Christine Chapon SPT8 Bam-Bam in pBM272 unknown... p7.4(757-4) SPT8
FB920 Christine Chapon "SPT8 Bam-Bam in pBM272, same as p7.4 in FB919 " unknown... p7.14(757-14) SPT8
FB921 Christine Chapon "SPT8 Bam-Bam in pBM272, opposite orientation from p7.4 in FB919 " unknown... p7.5(757-5) SPT8
FB922 Christine Chapon "SPT8 Bam-Bam in pBM272, same as p7.5 in FB921 " unknown... p7.15(757-15) SPT8
FB923 Dave Deitcher TG1.own... "JM101, Ecok
FB924 205 Martha Soto HB101.n... mutant of pDE75-2 ampR TRP1 CEN ARS SPT15
FB925 227 Martha Soto HB101.n... mutant of pDE75-2 ampR TRP1 CEN ARS SPT15
FB926 257 Martha Soto HB101.n... mutant of pDE75-2 ampR TRP1 CEN ARS SPT15
FB927 264 Martha Soto HB101.n... mutant of pDE75-2 ampR TRP1 CEN ARS SPT15
FB928 283 Martha Soto HB101.n... mutant of pDE75-2 ampR TRP1 CEN ARS SPT15
FB929 308 Martha Soto HB101.n... mutant of pDE75-2 ampR TRP1 CEN ARS SPT15
FB930 311 Martha Soto HB101.n... mutant of pDE75-2 ampR TRP1 CEN ARS SPT15
FB931 315 Martha Soto HB101.n... mutant of pDE75-2 ampR TRP1 CEN ARS SPT15
FB932 7 Martha Soto HB101.n... mutant of pCC11 SPT6 CEN URA3
FB933 6 Martha Soto HB101.n... mutant of pCC11 SPT6 CEN URA3
FB934 9 Martha Soto HB101.n... mutant of pCC11 SPT6 CEN URA3
FB935 13 Martha Soto HB101.n... mutant of pCC11 SPT6 CEN URA3
FB936 15 Martha Soto HB101.n... mutant of pCC11 SPT6 CEN URA3
FB937 16 Martha Soto HB101.n... mutant of pCC11 SPT6 CEN URA3
FB938 17 Martha Soto HB101 .... mutant of pCC11 SPT6 CEN URA3
FB939 18 Martha Soto HB101..... mutant of pCC11 SPT6 CEN URA3
FB940 19 Martha Soto HB101..... mutant of pCC11 SPT6 CEN URA3
FB941 22 Martha Soto HB101..... mutant of pCC11 SPT6 CEN URA3
FB942 Greg Prelich TB1.1..... pGP43 ampR LEU2
FB943 Greg Prelich TB1.1..... pGP44 ampR URA3
FB944 Greg Prelich TB1.1..... pGP45 ampR TRP1
FB945 Dan Finley HB101..... pUB200 UBI4 ampR
FB946 Dan Finley Make DNA from this strain if you want to cut at BclI site (enzyme is sensiti ve to methylation). FB432..... dam- pUB200 UBI4 ampR
FB947 Steve Buratowski/Sharp Lab "Ref: Cell 56, p. 549-561 " HB101..... pRW ampR
FB948 S. Ricupero "~1kb BglII to ClaI fragment of RSP4+ rescued from yeast, was cloned into puc 19 BamHI to AccI. All four restriction sites have been destroyed. " HB101..... pFW258 ampR ori lacZ
FB949 Sikorski/Hieter See sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS403 HIS3 ampR
FB950 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS404 TRP1 ampR
FB951 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS405 LEU2 ampR
FB952 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS406 URA3 ampR
FB953 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS413 CEN ARS HIS3 ampR
FB954 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS414 CEN ARS TRP1 ampR
FB955 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS415 CEN ARS LEU2 ampR
FB956 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS416 CEN ARS URA3 ampR
FB957 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS423 2micron HIS3 ampR
FB958 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS424 2micron TRP1 ampR
FB959 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS425 2micron LEU2 ampR
FB960 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS426 2micron URA3 ampR
FB961 Sikorski/Hieter For chromosome fragmentation. 1990 Cold Spring Harbor DH5alpha.. pYCF3 p299 URA3 SUP11 (see FB522)
FB962 Sikorski/Hieter For chromosome fragmentation. 1990 Cold Spring Harbor DH5alpha.. pYCF4 p300 URA3 SUP11 (see FB523)
FB963 CB100 Kim Arndt .d See enclosed sheet DO NOT GIVE OUT! See Science Aug87 vol.237 p.874; Nov89 vol.246 p.931 unknown CB100 BAS2 ampR ori
FB964 CB2 Kim Arndt . BAS1 inserted into pUC118. See enclosed sheet DO NOT GIVE OUT! See Scienc e Aug87 vol.237 p.874; Nov89 vol.246 p.931 unknown CB2 BAS1 ampR
FB965 AB289 Kim Arndt . See enclosed sheet DO NOT GIVE OUT! See Science Aug87 vol.237 p.874; Nov89 vol.246 p.931 .......... AB289 URA3 ampR ori
FB966 AB328 Kim Arndt . See enclosed sheet DO NOT GIVE OUT! See Science Aug87 vol.237 p.874; Nov.8 9 vol.246 p.931 .......... AB328 URA3 ampR ori
FB967 B238 Hinnebusch. See enclosed sheet for references! DO NOT GIVE OUT! .......... B238 URA3 CEN ARS ampR ori GCN4
FB968 B401 Hinnebusch. See enclosed sheets. DO NOT GIVE OUT! .......... B401 URA3 CEN ARS gcn4::LEU2 ampR ori
FB969 L433.1 kate and Fred HB101..... pFW265 AmpR URA3 2u spt8-lacz
FB970 L435.2 kate/fred . spt8R1+Xho1 in YEp354R1 + Sal1 HB101 pFW266 spt8-lacz AmpR URA3 2u
FB971 L437.1 kate and Fred EcoR1+Xho1 fusion HB101..... pFW267 AmpR URA3 2u spt8-lacz
FB972 pFW259 kate/fred RI-RI frag from pFW120 cloned into YEp353 HB101..... pFW259 SPT8RI/RI::lacZ ampR 2u URA3
FB973 pFW260 kate/fred RI-RI frag from pFW120 cloned into YEp354 RI-RI HB101..... pFW260 SPT8RI/RI::lacZ ampR 2u URA3
FB974 pFW261 kate/fred RI-RI frag from pFW120(middle band) cloned into YEp355 RI-RI. HB101..... pFW261 SPT8RI/RI::lacZ ampR 2u URA3
FB975 pFW262 kate/fred BamHI-RI frag from pFW263 cloned into YEp353 BamHI-RI HB101..... pFW262 SPT8Bam/RI::lacZ ampR 2u URA3
FB976 pFW263 kate/fred RI-BamHI frag from pFW120 (2nd band from the top) cloned into YEp354 RI-BamH I. HB101..... pFW263 SPT8RI/Bam::lacZ ampR 2u URA3
FB977 pFW264 kate/fred RI-BamHI frag from pFW120 (2nd band from the top) cloned into YEp355 RI-BamH I. HB101..... pFW264 SPT8RI/Bam::lacZ ampR 2u URA3
FB978 B835 Mark Rose this plasmid is YCp50 with the SalI site filled in creating a NEW PvuI site DB6507.... pMR78 URA3 ampR tetS
FB979 B836 Mark Rose this plasmid is YCp50 with the XhoI site filled in creating a NEW PvuI site. CEN function is not affected. DB6507.... pMR79 URA3 ampR tetR
FB980 RB316 Hieter/Schatz TUB3 (BglII frag of RB300) is inserted into the BamHI site of YEp24 (both si tes are destroyed). 2u piece includes origin of replication HB101..... pRB316 ampR URA3 2u TUB3
FB981 pJJ215 L. Prakash HIS3 BamHI frag in BamHI site of pUC18 (KpnI/XhoI of HIS3 is by H3/XbaI in v ector) REF: Yeast vol.6 #5 Sept-Oct90 p.363 unknown... pJJ215 HIS3 ampR lacZ ori
FB982 pJJ217 L. Prakash HIS3 BamHI frag in BamHI site of pUC18 (has opposite orientation as that of FB981) REF: Yeast vol.6 #5 Sept-Oct90 p.363 unknown... pJJ217 HIS3 ampR lacZ ori
FB983 pJJ242 L. Prakash URA3 H3 frag into H3 site of pUC18 (SmaI away from SmaI site) REF: Yeast vo l.6 #5 Sept-Oct90 p.363 MC1066.... pJJ242 URA3 ampR lacZ ori
FB984 pJJ244 L. Prakash URA3 H3 frag into H3 site of pUC18 (has oppostie orientation as that of FB98 3) REF: Yeast vol.6 #5 Sept-Oct90 p.363 MC1066.... pJJ244 URA3 ampR lacZ ori
FB985 pJJ246 L. Prakash "TRP1 EcoRI/RSal frag filled in, cloned into filled in XbaI site of pUC18 (Ec oRI site away from EcoRI site) REF: Yeast vol.6 #5 Sept-Oct90 p.363 " MC1066.... pJJ246 TRP1 ampR lacZ ori
FB986 pJJ248 L. Prakash "TRP1 EcoRI/RSal frag filled in, cloned into filled in XbaI site of pUC18 ( h as opposite orientaion as that of FB985) REF: Yeast vol.6 #5 Sept-Oct90 p .363 " MC1066.... pJJ248 TRP1 ampR lacZ ori
FB987 pJJ250 L. Prakash "LEU2 SalI/HpaI frag, filled in and cloned into filled in XbaI site of pUC18 (SalI site by SalI site) REF: Yeast vol.6 #5 Sept- Oct90 p.363 " MC1066.... pJJ250 LEU2 ampR lacZ ori
FB988 pJJ252 L. Prakash "LEU2 SalI/HpaI frag, filled in and cloned into filled in XbaI site of pUC18 (has opposite orientation as that of FB987) REF: Yeast vol.6 #5 Sept-Oct90 p.363 " MC1066.... pJJ252 LEU2 ampR lacZ ori
FB989 pCHY23 C. Hoffman BamHI-Nru1 of pCHY22 (lacZ) inserted into the XhoI site of pCHY21(FB748) Gen e 84:473-479 (1989) HB101..... pCHY23 ampR URA3
FB990 pFW268 Stephanie Ricupero spt15-6 mut. rescued by gap repair from FY174 using EcoRI/BamHI cut of FB877 (pDE59-1; in a pRS314 vector [FB659]). HB101..... pFW268 ampR CEN ARS TRP1 spt15-6
FB991 pFW269 Stephanie Ricupero spt15-8 mut. rescued by gap repair from FY177 using EcoRI/BamHI cut of FB877 (pDE59-1; in a pRS314 vector [FB659]). HB101..... pFW269 ampR CEN ARS TRP1 spt15-8
FB992 pGAD2 Rolf Sternglanz see enclosed sheet HB101..... pGAD2 ori ampR LEU2 2u
FB993 pKL14 Rolf Sternglanz see enclosed sheet HB101..... pKL14 TFIID ori ampR LEU2 2u
FB994 pMA424 Rolf Sternglanz see enclosed sheet HB101..... pMA424 HIS3 ampR 2u ori
FB995 pKL17 Rolf Sternglanz see enclosed sheet HB101 .... pKL17 TFIID HIS3 ampR ori 2u
FB996 pSAB6 Steve Brown 2.6kb BamHI-SacII HTA1-HTB1 frag into BamHI-SacII of pRS316 HB101..... pSAB6 HTA1-HTB1 CEN URA3 ampR
FB997 pRB58 M. Carlson SUC2 in BamH1 site of YEp24 HB101..... pRB58 SUC2 URA3 2u
FB998 pFW270 Stephanie Ricupero spt15-3 mut rescued by gap repair from FY200 using EcoRI/BamHI cut of FB877 (pDE59-1) HB101..... pFW270 ampR CEN ARS TRP1 spt15-3
FB999 pFW271 Stephanie Ricupero spt15-7 mut rescued by gap repair from FY259 using EcoRI/BamHI cut of FB877 (pDE59-1) HB101..... pFW271 ampR CEN ARS TRP1 spt15-7
FB1000 PB148 Patty Minehart SalI-RI frag of GLN1 cloned into SmaI of pSLFd178k (480bp) XL1.1..... pPM72 ampR GLN1 promoter (UAS and TATA(2)) CYC1::lacZ
FB1001 PB149 Patty Minehart "same SalI-RI frag as FB1000, but in reverse orientation " XL1.1..... pPM73 ampR GLN1 promoter (UAS and TATA) CYC1::lacZ
FB1002 SL96-33 Patty Minehart SalI-Hinf frag of GLN1 cloned into pSLFd178k (394bp) HB101..... pSL96-33 "ampR GLN1 promoter (UAS only, noTATA)
FB1003 pPM11 Patty Minehart GLN3 disrupted with LEU2 in pBR322. Note: cutting with AatII gives 2 frag o f approx. the same size. HB101..... pPM11 LEU2 ampR ori
FB1004 35BK Patty Minehart "GLN3 disrupted w/ LEU2 (same as in FB1003). AatII frag with Bam linkers on each end, then subcloned into Bam of KS+ plasmid (Bluescript). " HB101..... p35BK LEU2 ampR ori
FB1005 pLGd312s Cindy Keleher see enclosed sheets TB1.1..... pLGd312s see enclosed sheets
FB1006 JK1621 Cindy Keleher see enclosed sheets at FB1005 TB1.1..... pJK1621 see enclosed sheets at FB1005
FB1007 pLGd312-1 Cindy Keleher see enclosed sheets at FB1005 TB1.1..... pLGd312-1 see enclosed sheets at FB1005
FB1008 CK26 Cindy Keleher See enclosed sheets at FB1005 TB1.1..... pCK26 see enclosed sheets at FB1005
FB1009 CK30 Cindy Keleher See enclosed sheets at FB1005 TB1.1..... pCK30 see enclosed sheets at FB1005
FB1010 CK24 Cindy Keleher See enclosed sheets at FB1005 TB1.1..... pCK24 see enclosed sheets at FB1005
FB1011 AJ101 Cindy Keleher See enclosed sheets at FB1005 TB1.1..... pAJ101 see enclosed sheets at FB1005
FB1012 JK1521 Cindy Keleher See enclosed sheets at FB1005 TB1.1..... pJK1521 see enclosed sheets at FB1005
FB1013 CK22 Cindy Keleher See enclosed sheets at FB1005 TB1.1..... pCK22 see enclosed sheets at FB1005
FB1014 CK25 Cindy Keleher See enclosed sheets at FB1005 TB1.1..... pCK25 see enclosed sheets at FB1005
FB1015 pL50 Cindy Keleher See enclosed sheets at FB1005 TB1.1..... pL50 see enclosed sheets at FB1005
FB1016 CK23 Cindy Keleher See enclosed sheets at FB1005 TB1.1..... pCK23 see enclosed sheets at FB1005
FB1017 B1497 Truehart pBL101 with BglII(blunt)-BamHI(-542) replacing CYC1 sequences Sma-Bam HB101..... pSB231 ampR URA3 FUS1:lacZ CEN ARS ori
FB1018 B1498 Truehart = FB1017 (pSB231) with 2u HindIII frag from YEp24 replacing cen/ars HindIII frag HB101..... pSB234 ampR URA3 2u FUS1:lacZ
FB1019 pKA1 Karen Arndt SalI frag from YCp701 inserted at SalI site of pUC118. Orientation of inser t is not known. HB101..... pKA1 ampR his4-912d
FB1020 pKA2 Karen Arndt SalI frag from YCp701-R5 inserted at SalI site of pUC118. Orientation of in sert is not known. HB101..... pKA2 ampR his4-912dR5
FB1021 pKA3 Karen Arndt SalI frag from YCp701-R58 was inserted at the SalI site of pUC118. Orientat ion of insert is not known. HB101..... pKA3 ampR his4-912dR58
FB1022 pKA5 Karen Arndt SalI site was inserted 18bp upstream of 15-21 ATG by in vitro mutagenesis. T he 1164bp SalI-BamHI frag from mutagenized 2.4kb EcoRI- BamHI frag of 15-2 1 was subcloned into SalI/BamHI cut pSP64 HB101..... pKA5 ampR spt15-21
FB1023 pKA6 Karen Arndt EcoRI-BamHI frag rescued from FW969-1 containing the spt15-122 allele was su jected to oligo directed mut. to introduce a SalI site 18bp upstream of th e 15-122 ATG. The SalI-BamHI frag from the mut. M13 clone was then insert ed into pSP64. HB101..... pKA6 ampR spt15-122
FB1024 pKA7 Karen Arndt SpeI-BamHI frag (1245bp) of pDE32-1 was inserted into pSP64 that had been cu t with XbaI and BamHI. HB101..... pKA7 ampR SPT15+
FB1025 pKA9 Karen Arndt "using oligo-directed mut., an NdeI site was placed at the SPT15 ATG. The 11 43bp NdeI-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid.* *DO NOT GIVE OUT** " BL21(DE3). pLysS (is a plasmid carrying the T7 lysozyme gene =it is CmR). BL21(DE 3) is a lamda lysogen contai pKA9 ampR SPT15+
FB1026 pKA9 Karen Arndt "Using oligo directed mut., an NdeI site was placed at the SPT15 ATG. The 11 43bp NdeI-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid. SEE FB1025!** " HB101.E3). pKA9 ampR SPT15+
FB1027 pKA10/pet Karen Arndt "using oligo-directed mut, an NdeI site was placed at the spt15 ATG. The 114 3bp NdeI-BamHI frag. was inserted into pET3b, a T7 overexpressor plasmid. **SEE FB1025!** " BL21(DE3). pLysS (a plasmid carrying the T7 lysozyme gene; it is CmR) BL21(DE3) is a lmabda lysogen containi pKA10 AmpR spt15-122 CmR
FB1028 pKA10 Karen Arndt "using oligo-directed mut. an NdeI site was placed at the spt15 ATG. The 114 3bp frag was inserted into pET3b, a T7 overexpressor plasmid. **SEE FB1027 !** " HB101.E3). pKA10 AmpR spt15-122
FB1029 pKA11 Karen Arndt "using oligo-directed mut. an NdeI site was placed at the spt15 ATG. The 114 3bp NdeI-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid. **SEE FB1025!** " BL21(DE3). pLysS (a plasmid carrying the T7 lysozyme gene; it is CmR) BL21(DE3) is a lambda lysogen containi pKA11 AmpR spt15-21 CmR
FB1030 pKA11 Karen Arndt "using oligo-directed mut. an NdeI site was placed at the spt15 ATG. The 114 3bp Nde-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid. * *SEE FB1029!** " HB101.E3). pKA11 AmpR spt15-21
FB1031 pKA12 Karen Arndt The 2.4kb EcoRI-BamHI frag from pDE73-21 was cloned into pFW4 cut with EcoRI and BamHI HB101.E3). pKA12 AmpR URA3 SPT15
FB1032 pKA13 Karen Arndt ~6.6kb ClaI-SacI insert rescued from FW969 was cloned into pRS306. The ClaI -SacI insert contains the spt15-122 allele. HB101.E3). pKA13 AmpR URA3 spt15-122 (integrating)
FB1033 pKA14 Karen Arndt "spt15-301= L114F, it was introduced by site-directed mut. into the SalI-XbaI frag of pKA4, which was then introduced into pKA12/SalI-XbaI. " HB101..... pKA14 AmpR URA3 spt15-301 (L114F)
FB1034 pKA15 Karen Arndt "spt15-302= G119V, it was introduced by oligo-directed mut into pKA4 SalI-Xba I frag, which was subcloned into pKA12. " HB101..... pKA15 AmpR URA3 spt15-302 (G119V)
FB1035 pKA16 Karen Arndt "spt15-303= L205A, it was subcloned into pKA12 as XbaI-BamHI frag " HB101..... pKA16 AmpR URA3 spt15-303 (L205A)
FB1036 pKA17 Karen Arndt "spt15-304= L205I, it was created by oligo-directed mut. and was introduced i nto pKA12 as XbaI-BamHI frag. " HB101..... pKA17 AmpR URA3 spt15-304 (L205I)
FB1037 pKA18 Karen Arndt "spt15-305= L205K, it was made by oligo-directed mut. in M13 and was subclone d into pKA12 as XbaI-BamHI frag. " HB101..... pKA18 AmpR URA3 spt15-305 (L205K)
FB1038 pKA19 Karen Arndt spt15-306= G210V is subcloned into pKA12 as XbaI-BamHI frag. HB101..... pKA19 AmpR URA3 spt15-306 (G210V)
FB1039 pKA20 Karen Arndt spt15-307= L205F (spt15-122) and F207L. F207L was introduced into the spt15 -122 gene to make a double mut.; the mutagenized XbaI-BamHI frag from spt1 5-122 was then subcloned into pKA12. HB101..... pKA20 "AmpR URA3 spt15-307 (L205F, F207L)
FB1040 pKA21 Karen Arndt XbaI-BamHI frag fron spt15-122 was subcloned into pKA12 tp replace the wild type XbaI-BamHI frag. spt15-122= L205F. HB101..... pKA21 AmpR URA3 spt15-122
FB1041 pKA22 Karen Arndt spt15-308= L114F and L205F. The spt15-122 (L205F) XbaI-BamHI frag was subcl oned into pKA14 (contains spt15-301=L114F) to replace its XbaI-BamHI sec. pKA22 carries double L to F mutations in the repeats of the SPT15 gene. HB101..... pKA22 "AmpR URA3 spt15-308 (L114F, L205F)
FB1042 pKA23 Karen Arndt 2.2kb XbaI-HindIII frag containing LEU2 from pDE48-1 was inserted into pKA12 SpeI-HindIII to replace almost the entire SPT15 reading frame with LEU2.( =spt15 null). HB101..... pKA23 AmpR URA3 spt15-102dLEU2
FB1043 pKA24 Ricupero/Arndt "spt15-309= L114A, it was made by oligo directed mut of SalI-XbaI frag of pKA 4, it was then inserted into pKA12 as the SalI-XbaI frag. " HB101..... pKA24 AmpR URA3 spt15-309 (L114A)
FB1044 pKA25 Ricupero/Arndt "spt15-310= L114A, it was introduced by oligo-directed mut. into SalI-XbaI fr ag of pKA4; SalI-XbaI frag was then cloned into pKA12 to replace wt sequen ces. " HB101..... pKA25 AmpR URA3 spt15-310 (L114A)
FB1045 pKA26 Ricupero/Arndt spt15-311= L114K; it was made by oligo-directed mut. of pKA4 SalI-XbaI frag in M13MP18; the mutagenized SalI-XbaI frag was then cloned into pKA12 to r eplace wt seq. HB101..... pKA26 AmpR URA3 spt15-311 (L114K)
FB1046 pKA27 Ricupero/Arndt spt15-312= G119P; was made by oligo-directed mut. of pKA4 SalI-XbaI frag in M13mp18; the mutagenized frag was inserted as a SalI-XbaI piece into pKA12 to replace wt seq. HB101..... pKA27 AmpR URA3 spt15-312 (G119P)
FB1047 pKA28 Ricupero/Arndt spt15-313= K127A; it was made by oligo-directed mut. of pKA4 SalI-XbaI frag in M13mp18;the mutagenized SalI-XbaI frag was then subcloned into pKA12 to replace wt sequences. HB101..... pKA28 AmpR URA3 spt15-313 (K127A)
FB1048 pKA29 Ricupero/Arndt spt15-314= K127R; it was made by oligo-directed mut of pKA4 SalI-XbaI frag i n M13mp18; the mutagenized SalI-XbaI frag was then subcloned into pKA12 to replace the wt seq. HB101..... pKA29 AmpR URA3 spt15-314 (K127R)
FB1049 pKA30 Ricupero/Arndt "spt15-315= L205D; it was made by oligo-directed mut. of XbaI-BamHI frag of p KA4, it was then inserted into pKA12 as the XbaI-BamHI frag to replace wt seq. " HB101..... pKA30 AmpR URA3 spt15-315 (L205D)
FB1050 pKA31 Ricupero/Arndt spt15-316= G210P; it was made by oligo-directed mut. of pKA4 XbaI-BamHI frag in M13mp18; the mutagenized frag was then inserted as the XbaI-BamHIfrag of pKA12 to replace wt seq. HB101..... pKA31 AmpR URA3 spt15-316 (G210P)
FB1051 pKA32 Ricupero/Arndt spt15-317= K218A; it was made by oligo-directed mut of pKA4 XbaI-BamHI frag in M13mp18; the mutagenized frag was then cloned onto pKA12 as the XbaI-Ba mHI frag to replace wt seq. HB101..... pKA32 AmpR URA3 spt15-317 (K218A)
FB1052 pKA33 Ricupero/Arndt spt15-318= K218R; it was made by oligo-directed mut of pKA4 Xba-BamHI frag i n M13mp18; the mutagenized frag was then subcloned into pKA12 to replace w t seq. HB101..... pKA33 AmpR URA3 spt15-318 (K218R)
FB1053 pCC11 Christy ~6kb Bgl-Bgl frag from pCC27 cloned into Bam site of YCp50. SPT6+ is in ori entation I. HB101..... pCC11 AmpR CEN ARS URA3 SPT6+
FB1054 pSIN1 W. Kruger pSIN1 has SIN1/SPT2 on a 2.7kb RI-SphI fraf cloned into pMR366. (pMR366 is identical to YCp50 except the pBR322 origin has been replaced with pSC101' s thereby making DNA yeilds low. MH6....... pSIN1 SIN1/SPT2 AmpR URA3 CEN ARS
FB1055 pSZ26 Szostak/Wu REF: Plasmid 2:536-554 Szostak and Wu HB101..... pSZ26 AmpR rDNA-BglII-A
FB1056 pSPORT1 Gibco/BRL Clonig vector HB101..... pSPORT1 AmpR see insert
FB1057 pKK5-1 Brosius . "=plasmid for making RNA markers with E.coli RNA polymerase. Ref: Gene (1984 ) 27, p. 161-172 " HB101..... pKK5-1 AmpR
FB1058 pKK34-121 Brosius . "plamid for making RNA markers wiht E. coli RNA polymerase. Ref: MGG (1985) 199, p. 55-58 " HB101..... pKK34-121 AmpR
FB1059 pGAL4CG- Lue plasmid for yeast in vitro transcription. REF: Science 246 : 661 (1989) Lue et al. HB101..... pGAL4CG- AmpR
FB1060 pJR201 J. Rooney see notebook. HB101..... pJR201 spt15-201 AmpR CEN ARS TRP1
FB1061 pJR202 J. Rooney see notebook. HB101..... pJR202 spt15-202 AmpR TRP1 CEN ARS
FB1062 pJR203 J. Rooney see notebook. HB101..... pJR203 spt15-203 AmpR TRP1 CEN ARS
FB1063 pJR204 J. Rooney see notebook. HB101..... pJR204 spt15-204 AmpR TRP1 CEN ARS
FB1064 pJR205 J. Rooney SEE NOTEBOOK HB101..... pJR205 spt15-205 AMPR CEN ARS TRP1
FB1065 pJR206 J. Rooney SEE NOTEBOOK HB101..... pJR206 spt15-206 AmpR TRP1 CEN ARS
FB1066 pJR207 J. Rooney SEE NOTEBOOK HB101..... pJR207 spt15-207 AmpR TRP1 CEN ARS
FB1067 pJR208 J. Rooney SEE NOTEBOOK HB101..... pJR208 spt15-208 AmpR TRP1 CEN ARS
FB1068 pJR209 J. Rooney SEE NOTEBOOK HB101..... pJR209 spt15-209 AmpR TRP1 CEN ARS
FB1069 pJR210 J. Rooney SEE NOTEBOOK HB101..... pJR210 spt15-210 AmpR TRP1 CEN ARS
FB1070 pJR211 J. Rooney SEE NOTEBOOK HB101..... pJR211 spt15-211 AmpR TRP1 CEN ARS
FB1071 pJR212 J. Rooney SEE NOTEBOOK HB101..... pJR212 spt15-212 AmpR TRP1 CEN ARS
FB1072 pJR213 J. Rooney SEE NOTEBOOK HB101..... pJR213 spt15-213 AmpR TRP1 CEN ARS
FB1073 pRS104 R. Simpson See notebook for information in insert. HB101..... pRS104 ARS AmpR
FB1074 pRS109 R. Simpson see notebook for information in insert. HB101..... pRS109 REP3 AmpR
FB1075 CP32 C. Peterson =BglII-SmaI frag of SWI3 in YIp5 (for making Southern probe). See letter in notebook. unknown... CP32 SWI3 URA3 AmpR
FB1076 CP34 C. Peterson is in YEp24. SWI2 (SNF2) disruption allele was created by replacing the lg. BglII frag with HIS3 gene. See letter in notebook for information. unknown... CP34 swi2d::HIS3 AmpR URA3 2micron ori
FB1077 CP59 C. Peterson see letter in notebook for information. unknown... CP59 swi3d::TRP1 AmpR URA3
FB1078 BD39 C. Peterson "is in YIp5. swi1::LEU2 allele was constructed by replacing BamHI/BglII dele tion of SWI1 with LEU2. Digest with PstI, isolate ~1kb frag for one-step d isruption of SWI1. See notebook for letter. " HB101.n... BD39 swi1d::LEU2 AmpR URA3
FB1079 pDAD1 Pellman/Fink See notebook for more info. and polylinker sequence HB101..... pDAD1 AmpR URA3 2micron Gal10' promoter
FB1080 pDAD2 Pellman/Fink See notebook for more info. and polylinker sequence. HB101..... pDAD2 AmpR URA3 2micron GAL10' promoter
FB1081 pLR1 L. Rosen "from FB759 (pRB759), URA3 SacI frag cloned into SacI site of FB659 (pRS314) that had its PpuMI site filled in. See FB1082 (pLR2). " HB101..... pLR1 AmpR TRP1 URA3 CEN ARS
FB1082 pLR2 L. Rosen #NAME? HB101..... pLR2 AmpR TRP1 CEN ARS
FB1083 pGBT9 Stan Fields see notebook HB101..... pGBT9 TRP1 AmpR Gal4 (1-147)
FB1084 pMFH1 D. Gottschling REF: MCB vol 5 No 4M Apr85 p.610-618. See notebook HB101..... pMFH1 LEU2 URA3 AmpR 2micron DAM
FB1085 pDP6+DAM D. Gottschling REF: MCB vol5 No4 Hoekstra + Malone 1985 p.610-618 MCB vol6 No10 Hoekstra + Malone 1986 p.3555-3558. See sheets in notebook. HB101..... pDP6+DAM LYS2 DAM AmpR
FB1086 pRR46 D. Gottschling REF: MCB 7:1012 HB101..... pRR46 rad1::LEU2
FB1087 BM1541 Mark Johnston to cut out GAL4 use either BamHI + HindIII or BamHI + EcoRI; the entire gen e including its promoter is in these frags. See sheets in notebook. unknown... pBM1541 AmpR TRP1 CEN ARS GAL4
FB1088 pJR303 J. Rooney RI-Bam frag of spt15-203 colned into YIp5 vector as its Bam/RI frag. HB101.n... pJR303 URA3 AmpR spt15-203
FB1089 pJR305 J. Rooney Bam/RI frag containing spt15-205 cloned into YIp5 vector as its Bam/RI frag. HB101.n... pJR305 AmpR URA3 spt15-205
FB1090 pJR307 J. Rooney Bam/RI frag containing spt15-207 was cloned into YIp5 vector as its Bam/RI f rag. HB101.n... pJR307 AmpR URA3 spt15-207
FB1091 pJR309 J. Rooney Bam/RI frag containing spt15-209 was cloned into YIp5 vector as its Bam/RI f rag/ HB101.n... pJR309 AmpR URA3 spt15-209
FB1092 pJR311 J. Rooney Bam/RI frag containing spt15-211 was cloned into YIp5 vector as its Bam/RI f rag. HB101.n... pJR311 AmpR URA3 spt15-211
FB1093 pJR312 J. Rooney Bam/RI frag containing spt15-212 was cloned into YIp5 vector as its Bam/RI f rag. HB101.n... pJR312 AmpR URA3 spt15-212
FB1094 BDE115 D. Eisenmann =BDE115 in Dave's collection. HB101..... pDE40-4 SPT15 AmpR TRP1 CEN ARS
FB1095 WB101 Craig Peterson plasmid is in a YCp50 vector unknown... pWB101 URA3 AmpR HHT2 HHF2 CEN ARS
FB1096 pM883 David Stillman ***DO NOT GIVE OUT!!!*** (see sheet) pM883= SIN3 in YCp50. HB101.n... pM883 SIN3 URA3 AmpR CEN ARS
FB1097 pM1387 David Stillman ***DO NOT GIVE OUT!!!*** (see enclosed sheet) pM1387= SIN4 in pRS316 HB101.n... pM1387 SIN4 URA3 AmpR CEN ARS
FB1098 BBM11 Betsy Malone Sal-Xho of LEU2 in YEp13 inserted into Sal site of pBM8 HB101..... pBM11 spt16-102::LEU2 AmpR URA3
FB1099 BBM22 Betsy Malone Bam frag of spt16-102::LEU2 from pBM11 (FB1098) into Bam site of YEp24 HB101..... pBM22 spt16-102::LEU2 AmpR URA3 TetS 2micron ori
FB1100 BBM23 Betsy Malone H3-Sal frag of SPT16 (from pBM7) put into pBR322.****This frag is probably N ONfunctional---it was made as a NORTHERN PROBE**** HB101..... pBM23 SPT16 frag AmpR TetS ori
FB1101 BBM25 Betsy Malone ScaI-H3 frag of SPT4 into H3-NruI site of YCp50 HB101..... pBM25 SPT4 AmpR URA3 CEN ARS
FB1102 BBM26 Betsy Malone RI-PvuII of SPT4 into RI-SmaI of YEp355 ***makes an in frame SPT4-lacZ fusio n HB101..... pBM26 SPT4-lacZ AmpR URA3 2micron
FB1103 BBM27 Betsy Malone RI-H3 frag of SPT4 into RI-H3 of pUC19 HB101..... pBM27 SPT4 AmpR ori
FB1104 BBM33 Betsy Malone spt16-23 Bam frag into pRS314 (= a derivative of pBM13) Yeast strain=L722 a lias BM101 HB101..... pBM33 spt16-23 AmpR TRP1 CEN ARS ori
FB1105 BBM34 Betsy Malone spt16-35 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L723 a lias BM102 HB101..... pBM34 spt16-35 AmpR TRP1 CEN ARS ori
FB1106 BBM35 Betsy Malone spt16-99 Bam Frag into pRS314 (=a derivative of pBM13) Yeast strain= L724 a lias BM103 HB101..... pBM35 spt16-99 AmpR TRP1 CEN ARS ori
FB1107 BBM37 Betsy Malone spt16-133 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L725 alias BM124 HB101..... pBM37 spt16-133 AmpR TRP1 CEN ARS ori
FB1108 BBM39 Betsy Malone spt16-146 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L726 alias BM126 HB101..... pBM39 spt16-146 AmpR TRP1 CEN ARS ori
FB1109 BBM42 Betsy Malone spt16-175 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L727 alias BM129 HB101..... pBM42 spt16-175 AmpR TRP1 CEN ARS ori
FB1110 BBM44 Betsy Malone spt16-181 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L728 alias BM131 HB101..... pBM44 spt16-181 AmpR TRP1 CEN ARS ori
FB1111 BBM45 Betsy Malone spt16-185 Bam frag in pRS314 (= a derivative of pBM13) Yeast strain= L729 alias BM132 HB101..... pBM45 spt16-185 AmpR TRP1 CEN ARS ori
FB1112 BBM46 Betsy Malone spt16-197 Bam frag in pRS314 (= a derivative of pBM13) Yeast strain= L730 a lias BM133 HB101..... pBM46 spt16-197 AmpR TRP1 CEN ARS ori
FB1113 BBM47 Betsy Malone SPT4 with Amino acid #7 changed fron Cys to Ser. (in vitro mutagen. of M13 clone 106 with primer BM32. H3 frag subcloned into YCp50) HB101..... pBM47 spt4 (Cys7 to Ser) URA3 AmpR CEN ARS
FB1114 BBM49 Betsy Malone SPT4 with amino acid #7 changed from Cys to His---Has opp orientation as tha t of pBM48. (From in vitro mut. fo M13 clone 106 with primerBM33. H3 fra g subcloned into YCp50.) HB101..... pBM49 spt4 (Cys7 to His) URA3 CEN ARS AmpR
FB1115 BBM50 Betsy Malone SPT4 with amino acid #27 changed from Cys to Ser. (from in vitro mut. of M1 3 clone 106 with primer BM34. H3 frag subcloned into YCp50. HB101..... pBM50 spt4 (Cys27 to Ser) AmpR URA3 CEN ARS
FB1116 BBM51 Betsy Malone from gap repair of pJF42 in L331 HB101..... pBM51 spt4-289 AmpR URA3 CEN ARS
FB1117 BBM52 Betsy Malone from gap repair of pJF42 in L351 HB101..... pBM52 spt4-3 AmpR URA3 CEN ARS
FB1118 BBM53 Betsy Malone from gap repair of pJF42 in L374 HB101..... pBM53 spt4-6 AmpR URA3 CEN ARS
FB1119 BBM56 Betsy Malone NOTE: lerner fusion to the N terminus of SPT4. (from oligo dir. mut. of M13 clone 106 with BM28; H3 frag subcloned into YCp50.) HB101..... pBM56 lerner::SPT4 AmpR URA3 CEN ARS
FB1120 BBM57 Betsy Malone NOTE: lerner fusion to C terminus of SPT4 (from oligo dir mut. of M13 clone 106 with BM29; H3 frag was subcloned into YCp50. HB101..... pBM57 SPT4::lerner AmpR URA3 CEN ARS
FB1121 BBM61 Betsy Malone SPT4 with amino acid #27 changed from Cys to His. (from oligo dir mut. of M1 3 clone 106 with BM35; H3 frag subcloned into YCp50) HB101..... pBM61 spt4 (Cys27 to His) AmpR URA3 CEN ARS
FB1122 BBM65 Betsy Malone NOTE: lerner fusion to the N terminus of SPT4 (H3 frag from mut7C cloned in to H3 site of pCGS42) HB101 .... pBM65 lerner::SPT4 AmpR ori URA3 2micron
FB1123 BBM66 Betsy Malone NOTE: lerner fusion to the C terminus of SPT4 (H3 frag from mut88 cloned int o H3 site of pCGS42.) HB101..... pBM66 SPT4::lerner AmpR URA3 2micron ori
FB1124 BBM68 Betsy Malone insert SPT5 (H3 to KpnI) into YIp356R (Has partial SPT5 function; blue in coli and yeast) E. coli strain is SICK. TB1.1..... pBM68 SPT5-lacZ (H3 to KpnI) AmpR URA3
FB1125 BBM72 Betsy Malone NOTE: lerner fusion to the N terminus of SPT4 (H3 frag from FB1122 [pBM65] cloned into H3 sites of YEp13.) HB101..... pBM72 SPT4::lerner AmpR LEU2 2micron TetR
FB1126 BBM76 Betsy Malone NOTE: =Orientation II. (H3 frag from mut13C into pCGS42) HB101..... pBM76 HAI::spt4 (Cys7 to Ser) URA3 2micron AmpR
FB1127 BBM77 Betsy Malone NOTE: = Orientation I. (H3 frag from mut14C into pCGS42.) HB101..... pBM77 HAI::spt4 (Cys7 to His) URA3 2micron AmpR
FB1128 BBM79 Betsy Malone NOTE: = Orientation I (H3 frag from mut15A cloned into pCGS42.) HB101..... pBM79 HAI::spt4 (Cys27 to Ser) URA3 2micron AmpR
FB1129 BBM80 Betsy Malone NOTE: = Orientaion I (H3 frag from mut16B into pCGS42.) HB101..... pBM80 HAI::spt4 (Cys27 to His) URA3 AmpR 2micron
FB1130 BBM83 Betsy Malone NOTE: = Orientation II (H3 frag from mut18B into pCGS42.) HB101..... pBM83 HAI::spt4 (Cys24 to His) URA3 AmpR 2micron
FB1131 BBM84 Betsy Malone NOTE: =Orientation II (H3 frag from mut19B into pCGS42.) HB101..... pBM84 HAI::spt4 (Cys24; 27 to His) URA3 AmpR 2micron
FB1132 BBM89 Betsy Malone NOTE: = Orientation I (H3 frag from mut17C into pCGS42.) HB101..... pBM89 HAI::spt4 (Cys24 to Ser) URA3 AmpR 2micron
FB1133 pHB51 Henry Baker U. Fla TPI1= triose phosphate isomerase. H3-H3 frag with TPI1 (~4.4kb) in pUC18. HB101..... pHB51 AmpR TPI1
FB1134 pHB59 Henry Baker U. Fla. TPI1= triose phosphate isomerase. H3-SphI frag of TPI1 into pUC18. HB101..... pHB59 AmpR TPI1
FB1135 pMS39 Michele Swanson from a 7kb H3-EagI frag containing HAI-SPT6 ligated to pRS315 at H3+EagI TB1....... pMS39 HAI-SPT6 LEU2 CEN AmpR ARS
FB1271 pPK618 M. Grunstein REF: CELL 55:27-39 MH1....... pPK618 hhf2d(4-19) CEN URA3 ampR
FB1272 pUK499 M. Grunstein REF: EMBO 7:2211-2219 HB101..... pUK499 HHF2 CEN URA3 ampR
FB1273 pUK192 M. Grunstein REF: EMBO 11:3297-3306 HB101..... pUK192 (hht1-hhf1)d::LEU2 ampR
FB1274 pUK431 M. Grunstein REF: EMBO 11:3297-3306 HB101..... pUK431 (hht2-hhf2)d::HIS3 ampR
FB1275 pTaq-DNAP G. Hartzog Taq Polymerase expression vector REF: Anal Biochem 191: 396-400 HB101..... pTaq-DNAP ampR Taq. Pol. vector
FB1276 pJH359 R. Young REF: Nature 347: 493 contains 205bp MaeII DNA frag containing the INO1 U AS inserted at the XhoI site of UAS-less CYC1-lacZ fusion vector pJH304 unknown... pJH359 CYC1 TATA-lacZ INO1UAS ampR 2u
FB1277 pLGSD5 R. Young REF: Nature 347: 493 contains the GAL10 UAS inserted at the XhoI site to r eplace the CYC1 UAS in the CYC1-lacZ fusion vector pLGd2925 unknown pLGSD5 CYC1 TATA-lacZ GAL10-UAS ampR 2u
FB1278 pHYC3 R. Young REF: Nature 347: 493 contains HIS4 UAS inserted at the XhoI site to repla ce the CYC1 UAS in the CYC1-lacZ fusion vector pLG669-2 unknown... pHYC3 CYC1 TATA-lacZ HIS4-UAS ampR 2u
FB1279 pCT3 R. Young #NAME? unknown... pCT3 "URA3 ampR CEN ARS Bluescript KS polylinker T3,T7 promoters
FB1280 pCT15 R. Young =2.5kb frag containing SRB4 into ploylinker of pCT3 **see FB1279** unknown... pCT15 SRB4 URA3 CEN ARS ampR
FB1281 pCT48 R. Young "2.5kb frag containing srb4-1 into pCT3 **see also FB1280, FB1279** " unknown... pCT48 srb4-1 URA3 CEN ARS ampR
FB1282 pCT39 R. Young 1.9kb insert --- obtained by gap repair of SacI/XhoI of pCT32 (FB1283) unknown... pCT39 SRB5 URA3 CEN ARS ampR
FB1283 pCT32 R. Young "see also FB1282, FB1279 " unknown... pCT32 srb5-1 URA3 CEN ARS ampR
FB1284 pCT40 R. Young pCT40 (SRB6) obtained by gap repair of BalI/SphI in pCT29 (FB1285) unknown... pCT40 SRB6 URA3 CEN ARS ampR
FB1285 pCT29 R. Young "see also FB1284, FB1279 " unknown... pCT29 srb6-1 URA3 CEN ARS ampR
FB1286 pAB24 Chiron Corp. (T. Brake) ***K. lactis library*** REF: NAR vol.19 No.19: 5345-5350. See Yeast Library notebook DH5.own... pAB24 K. lactis library URA3 ampR
FB1287 pJH310 S. Henry . "REF: Hirsch + Henry (1986) MCB 6, 3320-3328 see also FB1288 and sheets in n otebook. " unknown pJH310 INO1 ampR SP6 promoter
FB1288 pJH311 S. Henry . "REF: Hirsch + Henry (1986) MCB 6, 3320-3328. See also FB1287 AND SHEETS IN NOTEBOOK " Unknown pJH311 INO1 ampR SP6 promoter
FB1289 RS426-TDS1 Buratowski. TDS1 is a high copy suppressor of YEpGAL-IID[R105]. Vector= pRS426 unknown pRS426-TDS1 TDS1 URA3 2micron ampR
FB1290 pL909 B. Berg (U. Chicago) REF: Apricio et al. CELL 66: 1279-1287 (1991) contains a 3.6kb ADE2 BamHI frag in YEp13. unknown... pL909 ADE2 ampR
FB1291 pdADE2 B. Berg (U. Chicago) REF: Aparicio et al. CELL 66:1279-1287(1991) Alani et al. Genetics 116:541 (1987) =ADE2::hisG URA3 disruption in bluescript derivative pV21 unknown... pdADE2 ADE2::hisGURA3 ampR
FB1292 pHB25 Henry Baker = 7.4kb SalI frag of the GCR region into which the 3.5kb BclI frag was repla ced by 3.5kb BglII LEU2 frag (in pBR322) unknown... pHB25 gcr::LEU2 ampR
FB1293 pHB45 Henry Baker BclI frag of GCR replaced with BamHI HIS3 frag into SalI of pBR322. See als o FB1292. unknown... pHB45 gcr::HIS3 ampR
FB1294 pLG36 L. Gansheroff FB422 plasmid cut with XbaI and SacI; insert LEU2 on Xba/Sac frag cut out of FB942 HB101.n... pLG36 ste12d::LEU2 URA3 ampR
FB1295 pBM290 Johnston . "REF: Yocum + Johnston (1984) Gene 32,75-82. =GAL80 disruption plasmid. GAL 80 gene is NOT present on this plasmid. See enclosed sheets. " unknown pBM290 URA3 SUP11 GAL80 flanking sequences ampR
FB1296 pBM1974 Johnston . "REF; Griggs + Johnston (1993) MCB 13, 4999-5009 See enclosed sheets. " unknown pBM1974 GAL4-CAT fusion URA3 ampR
FB1297 pmlc28ARG4 Szostak via C. Epstein ARG4 3.2kbp Pst frag in pmlc28 **See enclosed sheets** MC1061.... pmlc28Pst6ARG4 ARG4 CmR
FB1298 pmlc12ARG4 Szostak via C. Epstein Full plasmid name= pmlc12PstARG4BglIIFill (BamHI URA3) BglII site in ARG4 mutated to create ClaI site (in pmlc12) **see enclosed sheets** MC1061.... pmlc12PstARG4BglII arg4 URA3 CmR
FB1299 pACR1 Roger Brent DH5 (?).. pACR1 ACR1 ampR URA3
FB1300 pdACR1 Roger Brent DH5 (?) pdACR1 acr1::URA3 ampR LEU2
FB1301 LR1d1 Roger Brent GAL1 deleted UAS leaves XhoI site. NO LexA ops. this strain is the negati ve control for FB1302-FB1306. DH5 (?). LR1d1 GAL1dUAS-lacZ URA3 ampR 2micron
FB1302 pSH18-18 Roger Brent "see also FB1301, FB1303-1306. " DH5 (?).. pSH18-18 GAL1dUAS-lacZ URA3 2u ampR LexA ops. (6)
FB1303 pRB1840 Roger Brent "see also FB1301-1302, FB1304-1306 " DH5 (?).. pRB1840 GAL1dUAS-lacZ URA3 2u ampR LexA op.
FB1304 pSH18-34 Roger Brent "see also FB1301-1303, FB1305-1306 " DH5 (?).. pSH18-34 GAL1dUAS-lacZ URA3 2u ampR LexA ops (8)
FB1305 pSH18-8 Roger Brent "see also FB1301-1304, FB1306 " DH5 (?).. pSH18-8 GAL1dUAS-lacZ URA3 ampR 2u LexA ops. (4)
FB1306 SH1834dspe Roger Brent see also FB1301-1305 DH5 (?).. pSH18-34dspe GAL1dUAS-lacZ URA3 ampR integrating LexA ops.
FB1307 pSH17-4 Roger Brent unknown... pSH17-4 HIS3 LexA-GAL4 ampR 2u
FB1308 pCY40 Tom Stevens REF: MCB 10:3737 Use 4.3kb HindIII frag for disruption. MC1066.... pCY40 vat2d1::LEU2 ampR
FB1309 pGH2 Grant Hartzog MC1061.... pGH2 TRP1 ARS1 ampR (in SP72)
FB1310 pGH4 Grant Hartzog MC1061.... pGH4 SPT6 CEN ARS URA3 ampR
FB1311 pGH5 Grant Hartzog 3.33kb Bam-H3 frag from FB1087 into Bam-H3 cut pRS426 MH1.61.... pGH5 GAL4 ampR URA3 2u
FB1312 pGH6 Grant Hartzog 3.33kb Bam-H3 frag containing GAL4 into Bam-H3 cut pRS425 MH1.61.... pGH6 GAL4 ampR LEU2 2u
FB1313 pGH7 Grant Hartzog MC1061.... pGH7 SPT5 HIS3 ADH promoter ampR 2u
FB1314 pGH8 Grant Hartzog MH1.61.... pGH8 SPT5 TRP1 ampR 2u GAL1 promoter
FB1315 pGH9 Grant Hartzog "see also FB1313, FB1314 " MH1.61.... pGH9 SPT5 coding ampR
FB1316 pJK101 Roger Brent LacZ reporter with upstream UAS and intervening lexA operator. For Blockin g Assay. ***DO NOT DISTRIBUTE!!*** MC1061 pJK101 URA3 ampR GAL1-lacZ LexA binding downst. UAS
FB1317 pJG4-4 Roger Brent = version of pJG4-5 (FB1319) that lacks ATG-Nuc. loc. epitope tag-acidic act ivator. For two hybrid system. ***DO NOT DISTRIBUTE!!*** MH1 pJG4-4 TRP1 ampR 2u ori Gal1 promoter ADH terminator
FB1318 pJG4-6 Roger Brent = version of pJG4-5 (FB1319) that has ATG-epitope tag but lacks the nuclear localization signal and acidic activation domain. For two hybrid system. ***DO NOT DISTRIBUTE!!*** MH1 pJG4-6 ampR TRP1 2u ori Gal1 promoter ADH terminator
FB1319 pJG4-5 Roger Brent See enclosed sheets for more info. ***DO NOT DISTRIBUTE!!*** MC1061 pJG4-5 ampR TRP1 2u ori Gal1 promoter ADH terminator
FB1320 pJG4-5s Roger Brent See enclosed sheets for more info ***DO NOT DISTRIBUTE!!*** MH1 pJG4-5s ampR TRP1 2u ori Gal1 promoter ADH terminator
FB1321 pRFHM1 Roger Brent REF: Nature 350:250 ***DO NOT DISTRIBUTE!!*** MC1061.... pRFHM1 HIS3 ampR 2u deriv LexA 202+PL
FB1322 pEG202 Roger Brent = deriv. of LexA202+Pl see enclosed sheets for more info ***DO NOT DISTRIB UTE!!*** MC1061 pEG202 HIS3 2u ampR LexA
FB1323 DH10/P3 Grant Hartzog E. coli strain for propagating supF plasmids DH10/P3... "F- mcrA d(mrr-hsd RMS-mcrBC) 0 80d lacZdM15 dlacX74 deoR recA1 arad139 a(ara, leu)7697 galu galk
FB1324 pSR1-2 Shannon Roberts 7.3kb Cla-RI frag from pFW127 inserted into Cla-RI of pRS404 DH5 alpha. pSR1-2 SPT7 ampR TRP1
FB1325 pSR2-1 Shannon Roberts 3.1kb Sal-Spe frag cont. SPT8 from pFW120 inserted in Sal-Spe polylinker of pRS405 MH1.alpha. pSR2-1 SPT8 LEU2 ampR
FB1326 pSR3 Shannon Roberts #NAME? DH5 alpha. pSR3 URA3 2u ampR
FB1327 pSR4-5 Shannon Roberts constructed by cutting out 3kb Sal-Spe SPT8 frag from pMG1 and inserting it into Sal-Spe of pSR3 (FB1326) ***for epitope tagging SPT8*** DH5 alpha. pSR4-5 SPT8 URA3 2u ampR
FB1328 pSR5 Shannon Roberts 3kb SPT8 Sal-Spe frag into Sal-Spe of pRS426 DH5 alpha. pSR5 SPT8 URA3 2u ampR
FB1329 pSR6-3 Shannon Roberts SPT8 tagged at N term. DH5 alpha. pSR6-3 SPT8-myc epitope URA3 ampR 2u
FB1330 pSR7-3 Shannon Roberts 3.1kb Sal-Spe SPT8-N term myc epitope tagged prot. from pSR6-3 (FB1329) into Sal-Spe of pRS424 DH5 alpha. pSR7-3 SPT8-myc epitope TRP1 ampR 2u
FB1331 EB191 Elaine Elion STE12 tagged at C term. HB101.pha. pNC252 2u URA3 ampR GAL1 STE12-myc epitope
FB1332 pJS90 Marian Carlson REF: MCB 10:4744 HB101..... pJS90 ssn6d9URA3 ampR
FB1333 pLN113-3 Marian Carlson REF: MCB 7:3637 HB101..... pLN113-3 SSN6 2micron ampR URA3+
FB1334 pLexASNF2 Marian Carlson REF: PNAS 88:2687 HB101..... pLexASNF2 LexA-SNF2 2micron HIS3 ampR
FB1335 pLexASNF5 Marian Carlson REF: MCB 10:5616 HB101..... pLexASNF5 LexA-SNF5 2micron HIS3 ampR
FB1336 pLexASNF6 Marian Carlson REF: Genes + Dev. 6:1707 NOTE: this is LexA 1-202 + SNF6 according to B. L aurent. HB101..... pLexASNF6 LexA-SNF6 2micron HIS3 ampR
FB1337 pGP59 Greg Prelich = original BUR1+ clone from YCp50 Bam/Sau3A library. (Complements bur1-1 and bur1-2 HB101..... pGP59 ampR URA3 CEN ARS BUR1
FB1338 pGP73 Greg Prelich = original clone from YCp50 CEN library (Complements bur5-1) HB101..... pGP73 ampR URA3 CEN ARS copyII (HHT2-HHF2)
FB1339 pGP74 Greg Prelich = an original bur5-1 complementing plasmid isolated from YCp50 CEN library. HB101..... pGP74 ampR CEN ARS URA3 BUR5 (copyI=HHT1-HHF1)
FB1340 pGP89 Greg Prelich a 4.5kb EcoRI frag from pGP59 (FB1337) containing BUR1+ subcloned into EcoRI site of YIp5 HB101..... pGP89 ampR tetR URA3 BUR1frag.
FB1341 pGP103 Greg Prelich ~2250bp ClaI frag from pGP91 containing BUR1+ was put into pRS316 ClaI vecto r (=partial BUR1 clone---complements bur1-) HB101..... pGP103 ampR CEN URA3 BUR1
FB1342 pGP105 Greg Prelich a 1227bp TRP1 Bam/Bgl frag of YRp7 was put in place of the 170bp BglII frag in pGP103 (FB1341) to create a bur1d marked by TRP TB1 pGP105 ampR CEN URA3 bur1d1::TRP1
FB1343 pGP147 Greg Prelich a 6kb SphI frag of pGP74 (FB1339) containing BUR5 was subcloned into the Sph I site of YCp50 (Complements bur5-1) TB1.1..... pGP147 ampR CEN ARS URA3 HHT1-HHF1
FB1344 pGP150 Greg Prelich a 3kb KpnI-PvuII frag from pGP147 (FB1343) was cloned into the KpnI-SmaI sit es of pRS416 TB1.1..... pGP150 ampR CEN6 ARS1+4 URA3 HHT1
FB1345 pGP153 Greg Prelich 1.6kb SphI-ClaI frag from pGP147 (FB1343) was put into the SphI-ClaI sites o f YCp50. TB1.1..... pGP153 ampR CEN4 ARS1 URA3 HHT1-HHF1
FB1346 pGP154 Greg Prelich a 1.2kb EagI-KpnI frag from pGP147 (FB1343) was put into the EagI-KpnI sites of pRS416. TB1.1..... pGP154 ampR CEN6 ARS URA3 HHF1
FB1347 pGP156 Greg Prelich "a 5.4kb frag of pMS191 was put into the Bam-Hind3 sites of pRS305 to allow i ntegration at copyI, marked by LEU2, after SmaI linearization. " TB1.1..... pGP156 ampR LEU2 HHT1-HHF1
FB1348 pLG40 Lisa Gansheroff E. coli is SICK! See notebook. HB101..... pLG40 "his4-912d::URA3, lacZ ampR
FB1349 pLG41 Lisa Gansheroff E. coli is SICK! See notebook. HB101..... pLG41 "his4-912d::URA3, lacZ ampR
FB1350 pLG39 Lisa Gansheroff E. coli is SICK! See notebook. HB101..... pLG39 Ty912d44-lacZ fusion frag URA3 ampR
FB1351 pLG56 Lisa Gansheroff E. coli is SICK! See notebook. HB101..... pLG56 Ty912d44-lacZ fusion frag URA3 ampR
FB1352 pSAB3 Steve Brown SalI-SalI frag containing HTA1-HTB1 (5.7kb) into pRS316 TB1.1..... pSAB3 URA3 ampR CEN ARS HTA1-HTB1
FB1353 pSB17 Steve Brown = a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-46: dhH=G46D) See enclosed sheets in notebook. HB101..... pSB17 URA3 ampR CEN ARS HTA1-46
FB1354 pSB6 Steve Brown = a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-48: ddhA=G48D) se e enclosed sheets in notebook. HB101..... pSB6 URA3 ampR CEN ARS HTA1-48
FB1355 pSB23 Steve Brown =a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-61: dhV=A61T) see e nclosed sheets in notebook. HB101..... pSB23 URA3 CEN ARS ampR HTA1-61
FB1356 pSB7 Steve Brown =a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-90: ddhC=R90I) se e enclosed sheets in notebook. HB101..... pSB7 URA3 CEN ARS ampR HTA1-90
FB1357 pSB19 Steve Brown =a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-107: dhN=G107D) s ee enclosed sheets in notebook. HB101..... pSB19 URA3 CEN ARS ampR HTA1-107
FB1358 pSB9 Steve Brown =a derivative of pSAB3 (FB1352) mutagenized by HA (HTB1-105: ddHE=A105D) see enclosed sheets in notebook. HB101..... pSB9 URA3 CEN ARS ampR HTB1-105
FB1359 XL1-Blue Stratagene can use for M13 helper phage preps. See enclosed sheets in notebook. XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F` proAB lacIq ZdM15 tn10 tet-]
FB1360 pJL202 Schiestl .b replaces URA3 with a 1.1kb copy of HIS3 (complete deletion of URA3) MH1.Blue.. pJL202 ura3::HIS3 ampR
FB1361 pREP3X Enoch #NAME? MH1.Blue.. pREP3X nmt-1 promoter LEU2 ampR
FB1362 pM1329 Stillman 1.2kb PstI-RI subclone of CTS1 into pUC13 ***Northern Probe*** see sheets in notebook. unknown... pM1329 CTS1 ampR
FB1363 pM1591 Stillman contains 1.3kb upsteam of CTS1. See enclosed sheets. unknown... pM1591 CTS1-lacZ URA3 ampR
FB1364 pHIR1::URA Osley 1.4 BglII frag of HIR1 replaced with 1.1kb URA3 frag into YIp5. REF: Sherwo od et al MCB 1993 vol.13 no.1 p.28-38. HB101.n... pHIR1::URA3 HIR1::URA3 ampR URA3
FB1365 pHIR1::LEU Osley 1.4kb BglII frag of HIR1 replaced with 3kb LEU2 BglII frag REF: Sherwood e t al MCB 1993 vol.13 no.1 p.28-38. HB101.n... pHIR1::LEU2 HIR1::LEU2 ampR URA3
FB1366 pGEM150- Howley lab Bam-H3 frag of E6-AP in pGEM1 REF: Huibreqtse et al MCB 13:775-784 1993 HB101.n... pGEM150-120 E6-AP ampR
FB1367 pFW272 Stephanie Ricupero Bam-H3 frag containing E6-AP from FB1366 cloned into BglII-H3 of FB1080 (pDA D2) HB101.n... pFW272 ampR URA3 2micron E6-AP GAL10 promoter
FB1368 pRB1027 Roger Brent REF: Cell 43:729-736 HB101.n... pRB1027 PADH1-LexA-GAL4 fusion 2micron LEU2 ampR
FB1369 pAAH5 Roger Brent HB101.n... pAAH5 PADH1vector 2micron LEU2 ampR
FB1370 p1107 Roger Brent derived from pLG670Z (FB1373) HB101.n... p1107 LexAop CYC1-TATA-LacZ fusion URA3 ampR 2micron
FB1371 pRB1345 Roger Brent vector= pAAH5 (FB1369) HB101.n... pRB1345 PADH1-LexA-GCN4 fusion 2micron ampR LEU2
FB1372 pJLb-17b3 Roger Brent HB101.n... pJLb-17b3 GAL4 binding site CYC1-TATA-LacZ URA3 2micron ampR
FB1373 pLG670Z Roger Brent *** NO UAS *** REF: PNAS 78 2199-2203 (1981) HB101.n... pLG670Z CYC1-TATA-LacZ URA3 ampR 2micron
FB1374 Bd1128 G. Mikesell = pGAL::HIS3 in YCp50 REF: Genes + Dev. 5:1183-1190 HB101.n... Bd1128 pGAL::HIS3 ampR CEN ARS URA3
FB1375 Bd824 Mikesell =pGAL::SWI4 in YCp50 REF: Genes and Dev. 5:1183-1190 HB101..... Bd824 pGAL::SWI4 URA3 CEN ARS ampR
FB1376 pSR9.15 S. Roberts MH1.1..... pSR9.15 SPT8 CEN URA3 ampR
FB1377 pBBB2 Barbara Berg =NdeI-BamHI frag containing SPT3 from pDE118-8 into NdeI-BamHI of pET-3C (FB 894) SEE SHEETS IN NOTEBOOK. BL21(DE3). see enclosed sheets for info on host. Bl21(DE3) also contains pLysS w hich is CmR. pBBB2 T7 promoter ampR SPT3 CmR
FB1378 pBBB9 Barbara Berg PvuII-SalI frag containing rsp5 from pFW201 subcloned into pRS316 VJS533.3). #NAME? pBBB9 ampR CEN ARS URA3 rsp5 PvuII-SalI frag
FB1379 pBBB10 Barbara Berg BamHI-HindIII frag containing rsp5 from pFW201 subcloned into pRS316 VJS533.3). #NAME? pBBB10 ampR CEN ARS URA3 rsp5 BamHI-HindIII frag
FB1380 pBBB11 Barbara Berg BamHI-BamHI frag containing rsp5 from pFW201 subcloned into pRS316 VJS533.3). #NAME? pBBB11 ampR CEN ARS URA3 rsp5 BamHI-BamHI frag
FB1381 pBBB12 Barbara Berg SalI-HindIII frag containing rsp5 from pFW201 subcloned into pRS316. ***NOT E: construct may be incorrect*** VJS533.3). #NAME? pBBB12 ampR CEN ARS URA3 rsp5 SalI-HindIII frag
FB1382 pBBB13 Barbara Berg KpnI-HindIII frag containing rsp5 from pFW201 subcloned into pRS316. ***NOT E: constrcuct may be incorrect!*** VJS533.3). #NAME? pBBB13 ampR CEN ARS URA3 rsp5 KpnI-HindIII frag
FB1383 pBBB14 Barbara Berg BamHI-SalI frag containing rsp5 from pFW201 subcloned into pRS316 VJS533.3). #NAME? pBBB14 ampR CEN ARS URA3 rsp5 BamHI-SalI frag
FB1384 pSH2-1 Brent lab REF: Cell 51:113; 1987 Cell 43:729; 1985 **LexA polylinker vector** unknown.). pSH2-1 ADH1promoter-lexA ampR 2micron HIS3
FB1385 KB125 Ricupero/Arndt ~1.9kb SpeI frag containing 2micron origin was excised from p1107 (FB1370) a nd plasmid was religated. HB101..... pKA113 LexA Op CYC1-TATA-lacZ URA3 ampR integrating
FB1386 pAS1-CHY2 Steve Ellidge for 2 hybrid experiments MH1.1..... pAS1-CHY2 ampR 2micron CYH2 TRP1 ADHp-GAL4-HAI fusion
FB1387 YEpCHY1 Elion lab plasmid ~5.4kb of cyh2R gene in YEp24 **cycloheximide resistant** MH1.1..... YEpCHY1 URA3 ampR 2micron cyh2R
FB1388 pSR10-1 Shannon Roberts NOTE: used for interaction trap. See enclosed sheets. MH1.1..... pSR10-1 ampR HIS3 2micron LexA(1-202)-SPT8(35-602)
FB1389 pSR11-8 Shannon Roberts NOTE: used for interaction trap. See enclosed sheets. MH1.1..... pSR11-8 ampR TRP1 2micron B42-SPT8(35-602)
FB1390 pJM124myc Eun-Chung Park REF: Embo vol.11 No.6 p.2087-2093 (1992) unknown... pJM124myc NAT-myc LEU2 ampR 2micron
FB1391 ARDmyc Eun-Chung Park REF: Embo Vol.11 No.6 p.2087-2093 (1992) unknown... YEp24ARDmyc URA3 ARDmyc ampR 2micron
FB1392 pmcm1-1 M. Harris 3.3kb SphI-BamHI frag containing mcm1-1 inserted into Yip5. HB101.n... pmcm1-1 mcm1-1 ampR URA3
FB1393 pJH662 Jim Haber see sheets. BJH5..n... pJH662 ampR MATalpha tsm1::LEU2
FB1394 pJH569 Jim Haber see sheets. BJH2..n... pJH569 URA3 ampR TSM1 MATa inc.
FB1395 pSV14 Roger Brent GAL4 17mer is 178bp upstream of CYC1 TATA HB101.n... pSV14 GAL4 17mer CYC1-TATA-lacZ fusion URA3 ampR 2micron
FB1396 pRR330 Louis Prakash =plasmid for RAD9 knockout. REF: MCB 1989 Vol.9 No.5 p.1882 unknown... pRR330 RAD9::URA3 ampR
FB1397 pGN1262 Jef Boeke for 1-step deletion-insertion of SPT21 (cut with XhoI and transform) XL-Blue... pGN1262 URA3 ampR SPT21
FB1398 pLG72 Lisa Gansheroff insert= HindIII frag from pDBE6 containing unstable derivative of U6 gene v ector= pRS306 cut with HindIII ***DO NOT GIVE OUT--SEE SHEET!*** HB101.e... pLG72 U6 unstable derivative ampR URA3
FB1399 pLG73 Lisa Gansheroff same construct as pLG72 (FB1398) but insert is in opposite direction. ***DO NOT GIVE OUT--SEE SHEET!*** HB101.e... pLG73 U6 unstable derivative ampR URA3
FB1400 pLG74 Lisa Gansheroff insert= HindIII frag from pDBE6 containing an unstable (mRNA) derivative of U6 gene. vector= pRS316 cut with HindIII ***DO NOT GIVE OUT--SEE SHEET!* ** HB101.e... pLG74 U6 unstable derivative ampR URA3 CEN ARS
FB1401 pLG75 Lisa Gansheroff same construct as pLG74 (FB1400) but insert is in the opposite orientation. ***DO NOT GIVE OUT--SEE SHEET!*** HB101.e... pLG75 U6 unstable derivative ampR URA3 CEN ARS
FB1402 pLG76 Lisa Gansheroff insert= EagI-SalI frag from FB1148 containing N terminal half of SPT7 vect or= plasmid FB374 (C term. half of SPT7 in YCp50) cut with EagI and SalI. HB101.e... pLG76 SPT7 CEN URA3 ampR
FB1403 pLG77 Lisa Gansheroff insert= EagI-SalI frag from FB1149 containing N term. half of SPT7 with brom odomain deleted vector= plasmid of FB374 (C term. half of SPT7 in YCp50) cut with EagI and SalI. HB101.e... pLG77 spt7 bromodomain deletion CEN URA3 ampR
FB1404 pLG78 Lisa Gansheroff insert= EagI-SalI frag from FB1148 containing N term. half of SPT7 vector = plasmid of FB384 (C term. half of SPT7 in YIp5) cut with EagI and SalI HB101.e... pLG78 ampR URA3 SPT7
FB1405 pLG79 Lisa Gansheroff insert= EagI-SalI frag from FB1149 containing N term. half of SPT7 with Brom odomain deleted vector= plasmid of FB384 (C term. half of SPT7 in YIp5) cut with EagI and SalI HB101.e... pLG79 spt7 bromodomain deletion ampR URA3
FB1406 D16pUC13+ MaryAnn Osley =deletion 16prime (D16prime) with a XhoI site ***SalI site is gone*** =TRT 1 promoter with negative element deleted (region is marked by URA3+ insert ed downstream of H2B HB101.e... D16pUC13+ D16prime ampR
FB1407 pMS18 Michelle Swanson pRS316 + 3.2kb NruI frag from pMS4 containing SPT5. REF: Swanson et al MCB vol.11 no.6 June 91 p.3009-3019.*NOT FUNCTIONAL COPY OF SPT5* unknown... pMS18 URA3 ampR CEN ARS SPT5
FB1408 pMS37 Michelle Swanson YCp50 cut HindIII and NruI + pMS4 HindIII-PvuII frag. REF: Swanson et al M CB vol.11 no.6 June 91 p.3009-3019 *NOT A FUNCTIONAL COPY OF SPT5* unknown... pMS37 URA3 ampR SPT5
FB1409 MB81 Michelle Swanson 2.6kb SalI frag containing SacB cloned into SalI site of pSELECT (now known as pALTER) *Strain is Amp SENSITIVE!* JM109.n... pMB81 SacB ori TetR ampS SP6 T7promoter
FB1410 MB88 Michelle Swanson = in vitro mutagenesis of PMB81 (FB1409). JM109.n... pMB88 SacB-BamHI mutant ampR TetR ori Sp6 T7promoter
FB1411 pRS12 Louis Prakash REF: MCB 1989 vol.9 no.5 p.1882-1896 unknown... pRS12 RAD9 2micron LEU2 ampR
FB1412 pSR17.9 Shannon Roberts =pBR322 without AatII site at position #4168 MH1.own... pSR17.9 ampR Tet ori
FB1413 pSR8.1 Shannon Roberts ~3.1kb SalI-SpeI frag containing myc-tagged SPT8 cloned onto SalI-SpeI sites of pRS314 HB101.n... pSR8.1 TRP1 ampR myc-SPT8
FB1414 pCC528 Clarence Chan 1.1kb XhoI-HpaI frag from witnin LYS2 cloned into SalI-NruI sites of YIp5 S ee enclosed sheets. unknown pCC528 lys2 URA3 ampR
FB1415 pCC546 Clarence Chan 1.1kb XhoI-HpaI internal frag of LYS2 cloned into the XhoI-SmaI sites of pRS 305 See enclosed sheets. unknown pCC546 lys2 LEU2 ampR
FB1416 pCC8 Clarence Chan REF= Genetics 135: 677=691 Nov1993 pCC8=pRB1209-- see enclosed sheets unknown pCC8 leu2 URA3 ampR
FB1417 pCC35 Clarence Chan REF= Genetics 135: 677-691 Nov1993 pCC35= pRB1210-- see enclosed sheets. unknown pCC35 lys2 HIS3 ampR
FB1418 pSR18.3 Shannon Roberts 2.4kb SphI LexA-SPT3 frag from pLG67 cloned into SphI site of pSR17.9 (=FB14 12: pBR322 - AatII site) DH5alpha.. pSR18.3 LexA-SPT3 ampR
FB1419 pLexA-GCN4 L. Guarente ADH1p-LexA1-202-GCN49-172-ADH1term on ~2.8kb BamHI frag inserted into pRS316 BamHI site. (orientation of insert unknown) REF: Pina et al (1993) MCB: 13 p.5981 HB101..... pLexA-GCN4 ADH1p LexA-GCN4 CEN URA3 ampR
FB1420 LexA-Bicoi R. Brent . full length bicoid (AA2-289) fused to lexA1-202 ***activates transcription** * REF: Hanes and Brent (1991) Science 251:426-430 HB101..... pLexA-Bicoid LexA-Bicoid ampR HIS3 2micron
FB1421 pLexA-GAL4 L.Guarente ADH1p-lexA1-202-GAL4769-881-ADHterm on ~2.8kb BamHI frag in pRS316 REF: Pi na et al (1993) MCB:13 p.5981 HB101..... pLexA-GAL4 ADH1p lexA-GAL4 URA3 CEN ampR
FB1422 pLexA-HAP4 L. Guarente ADH1p-lexA1-202-HAP4330-554-ADHterm on ~2.8kb BamHI frag inserted into BamHI of pRS316 (orientation of insert unknown) REF: Pina et al (1993) MCB:13 p.5981 HB101..... pLexA-HAP4 ADH1p LexA-HAP4 CEN ampR URA3
FB1423 pLW20 Lena Wu HB101..... pLW20 ADH1p LexA-Bicoid LEU2 ampR integrating
FB1424 pLW23 Lena Wu ~2.8kb BamHI frag (from FB1421) containing ADH1p-lexA1-202-GAL4769-881-ADHte rm inserted into BamHI site of pRS425 (see FB1421 for ref.) HB101..... pLW23 ADH1p LexA-GAL4 2micron ampR LEU2
FB1425 pRS7.1Bgl2 K. Hansen BglII-BglII frag containing MOT1 in BamHI site of pRS316 unknown... pRS7.1BglII MOT1 ampR CEN ARS URA3
FB1426 pYEp7.1Bgl K. Hansen BglII-BglII frag containing MOT1 into YEp351 unknown... pYEp7.1BglII MOT1 ampR LEU2 2micron
FB1427 pKA44 Karen Arndt site directed mutagenesis of pKA41 with oligo KA47 (to destroy the delta TAT AAA) vector- pRS306. HB101..... pKA44 ampR his4-912d-t4 (PstI) URA3 integrating plasmid
FB1428 pKA78 Karen Arndt contains mutated SPT15 gene (spt15-320) generated by PCR mutagen. of SpeI-Ba mHI; SpeI-BamHI frag then subcloned into pKA75 to replace WT sequence. sp t15-320 A.A. change= R171P MH1.1..... pKA78 ampR CEN ARS TRP1 spt15-320
FB1429 pKA82 Karen Arndt "contains mutated SPT15 gene (spt15-324) generated by PCR mut. of SpeI-BamHI frag.; SpeI-BamHI frag then subcloned into pKA75 to replace WT sequence. spt15-324 AA change= E44G, R196G " MH1.1..... pKA82 ampR CEN ARS TRP1 spt15-324
FB1430 pKA86 Karen Arndt contains mutant SPT15 gene (spt15-328) generated by PCR mut. of SpeI-BamHIfr ag; SpeI-BamHI frag then subcloned into pKA75 to replace WT sequence. spt 15-328 AA change= P109A MH1.1..... pKA86 ampR CEN ARS TRP1 spt15-328
FB1431 pKA92 Karen Arndt contains mutant SPT15 gene (spt15-330) generated by PCR mutagenesis of SpeI- BamHI frag; SpeI-BamHI frag then subcloned into pKA75 to replace WT sequen ce. spt15-330 AA change= V71A MH1.1..... pKA92 ampR CEN ARS TRP1 spt15-330
FB1432 pKA94 Karen Arndt contains mutant SPT15 gene (spt15-332) generated by PCR mut. of SpeI-BamHI f rag; SpeI-BamHI frag then subcloned into pKA75 to replace WT sequence. sp t15-332 aa change= P109Q MH1.1..... pKA94 ampR CEN ARS TRP1 spt15-332
FB1433 pKA98 Karen Arndt contains mutant SPT15 gene (spt15-336) generated by PCR mut. of SpeI-BamHI f rag; SpeI-BamHI frag then subcloned into pKA75 to replace WT sequence. sp t15-336 AA change= F116Y and 1bp deleted in noncoding region. MH1.1..... pKA98 ampR CEN ARS TRP1 spt15-336
FB1434 pKA100 Karen Arndt "contains mutant SPT15 gene (spt15-338) generated by PCR mut. of SpeI-BamHI f rag; SpeI-BamHI frag then subcloned into pKA75 to replace WT sequence. sp t15-338 AA change= E27G, V161A " MH1.1..... pKA100 ampR CEN ARS TRP1 spt15-338
FB1435 pKA101 Karen Arndt contains mutant SPT15 gene (spt15-339) generated by PCR mut. of SpeI-BamHI f rag; SpeI-bamHI frag then subcloned into pKA75 to replace WT sequence. sp t15-339 AA change = E129K MH1....... pKA101 ampR CEN ARS TRP1 spt15-339
FB1436 pKA103 Karen Arndt contains mutant SPT15 gene (spt15-341) generated by PCR mut. of SpeI-BamHI f rag; SpeI-BamHI frag then subcloned onto pKA75 to replace WT sequence. sp t15-341 AA change= N159D MH1....... pKA103 ampR CEN ARS TRP1 spt15-341
FB1437 pKA75 Karen Arndt 2.4kb EcoRI-BamHI frag from pDE28-6 was inserted into EcoRI-BamHI sites of p KA70. (pKA70= pRS314 with SpeI site destroyed.) HB101..... pKA75 ampR CEN ARS TRP1 SPT15
FB1438 pKA61 Karen Arndt 3.1kb SalI frag from RD1 clone (RTF1) pKA45 was clone dinto the SalI site of pRS316 ***does not complement rd1-*** TB1.1..... pKA61 ampR CEN ARS URA3 RTF1
FB1439 GM48 G. Duyk contains NO dam or dcm methylases (from NEB catalogue) GM48 F- thr leu thi lacY galK ara fhuA tsx dam dcm SUPE44
FB1440 .B1.1..... pT2
FB1441 .B1.1..... pT1-3B
FB1442 .B1.1..... pIP5469
FB1443 pCC15 Christy ~6kb Bgl-Bgl frag from pCC27 cloned into the BamHI site of YEp24 HB101..... pCC15 SPT6 ampR URA3 2micron
FB1444 pHIBBB90 ATCC (#84835) human SPT4 HindIII-NotI clone XL1-Blue.. pHIBBB90 hSPT4 ampR
FB1445 p37/7 C. Connelly see enclosed sheets unknown... p37/7 ctf4d3::URA3 ampR LEU2 CEN ARS
FB1446 pPW58 Varshavsky see enclosed sheets MH1.own... pPW58 Ub-DHFRts-HAI epitope ampR
FB1447 pPW66R Varshavsky see sheets enclosed with FB1446 GM48.wn... dam- dcm- pPW66R cdc28-Ub-DHFRts-HAI fusion URA3 ampR
FB1448 pJDubrd4-B Varshavsky see sheets enclosed with FB1446 MH1..wn... pJDubrd4-B ubr1d::HIS3 ampR
FB1449 p180 Hieter HindIII clone that contains CEN10 in YRp14/ARS (centromere selection vector) HB101.n... p180 CEN10 SUP11 URA3 ampR ARS1
FB1450 p181 Hieter HindIII clone that contains CEN1 in YEp14/ARS (centromere selection vector) HB101.n... p181 CEN1 SUP11 URA3 ampR ARS1
FB1451 p200 Hieter HindIII clone that contains CEN7 in YRp14/ARS (centromere selection vector) HB101.n... p200 CEN7 SUP11 URA3 ampR ARS1
FB1452 p201 Hieter HindIII clone that contains CEN11 in YRp14/ARS (centromere selection vector) HB101.n... p201 CEN11 SUP11 URA3 ampR ARS1
FB1453 p202 Hieter HindIII clone containing CEN15 in YRp14/ARS (centromere selection vector) HB101.n... p202 CEN15 SUP11 URA3 ampR ARS1
FB1454 p203 Hieter HindIII clone containing CEN14 in YRp14/ARS (centromere selection vector) HB101.n... p203 CEN14 SUP11 URA3 ampR ARS1
FB1455 pMR102 Grunsein lab see enclosed sheets HB101.n... pMR102 URA3 CEN ARS ampR GAL10promoter-HHT2 GAL1promoter-HHF2
FB1456 pBM30 Betsy Malone RI-H3 spt4 frameshift from pBM28 cloned into RI-H3 of YCp50 HB101.n... pBM30 spt4 frameshift URA3 CEN ARS ampR
FB1457 pLG80 Gansheroff see enclosed sheet. HB101.n... pLG80 his4-912d G-less cassette ampR
FB1458 pD567 V. Lundblad see enclosed sheet HB101..... pD567 ARG4 ampR
FB1459 pWJ487 J. Smith SEE ALSO FB1479 FOR DAM- HOST. see enclosed sheets in notebook. DH5alpha.. pWJ487 MET14 URA3 ampR
FB1460 pWJ488 J. Smith SEE ALSO FB1480 FOR DAM- HOST. See sheets in notebook enclosed with FB1459! ! DH5alpha.. pWJ488 met14D(HpaI-SalI) URA3 ampR
FB1461 pNPS108 Dean Dawson 3.2kb PstI frag containing ARG4 cloned into the PstI site of polylinker in p MLC12. See enclosed sheets. DH5alpha.. pNPS108 ARG4 URA3 CmR
FB1462 pNPS250 Dean Dawson contains same PstI frag as in FB1461 (pNPS108) except a 2.1kb HpaI frag has been replaced by URA3. See enclosed sheets. DH5alpha.. pNPS250 arg4DHpaI URA3 CmR
FB1463 pGH77 Hartzog/ Hovasse RI-XhoI frag containing hSPT4 from pGH75 (FB1465) cloned into pJG4-6 DH5alpha.. pGH77 hSPT4 TRP1 ampR 2micron
FB1464 pGH78 Hartzog/ Hovasse RI-XhoI frag containing SPT4 from pGH76 (FB1466) cloned into pJG4-6 DH5alpha.. pGH78 SPT4 TRP1 ampR 2micron
FB1465 pGH75 Hartzog/ Hovasse RI-XhoI PCR cloned hSPT4 into RI-XhoI sites of pRS406 (Seq. confirmed) HB101.ha.. pGH75 ampR URA3 GAL1-hSPT4
FB1466 pGH76 Hartzog/ Hovasse RI-XhoI PCR cloned SPT4 into RI-XhoI sites of pRS406 (Seq. confirmed) HB101.ha.. pGH76 ampR URA3 GAL1-SPT4
FB1467 pGH80 Hovasse spt4 with Cys7 to Ser mutation made by PCR fron BM construct (FB1113). Vect or= pRS406. Has been confirmed by seq. HB101.ha.. pGH80 ampR URA3 spt4-C7S
FB1468 pGH81 Hovasse spt4 with Cys24 to His and Cys27 to His double mutation made by PCR from BM construct (FB1131) vector= pRS406 (confirmed by seq.) HB101..... pGH81 "ampR URA3 spt4-C24H,C27H
FB1469 pGH82 Hovasse spt4 with Cys24 to Ser mutation made by PCR from BM construct (FB1132) vect or= pRS406 (confirmed by seq.) HB101..... pGH82 ampR URA3 spt4-C24S
FB1470 hbc211 Jun Takeda ***DO NOT DISTRIBUTE!!*** = human SPT4 homologue REF: Human Molec. Genet ics 2(11) 1793 (1993) SOLR...... hbc211 ampR hSPT4 homologue
FB1471 yk45f2 Y Kohara (Gene Library lab) 3.7kb cDNA insert can rescue plasmid with helper phage! PLK-F'.... yK45f2 lambda lysogen yK45f2 C. elegans SPT5 homologue
FB1472 yK37h3 Y Kohara (Gene Library Lab) 3.0 kb cDNA insert can rescue plasmid with helper phage! PLK-F'.... yK37h3 lambda lysogen yK37h3 C. elegans SPT5 homologue
FB1473 pEMBL-GST Elion Lab REF= Yeast 9:715 DH5alpha.. pEMBL-GST ampR URA3 leu2-d (GAL1-10 UAS)-cyc1-GST
FB1474 pGH83 Hartzog "SPT5 in pEG(KG) complements spt5D for life, spt5-276 cs defect, and spt5-19 4 ts-/+ defect. " HB101..... pGH83 ampR Leu2-d URA3 2micron SPT5 (GAL1 UAS)-cyc1-GST
FB1475 pGH86 Hartzog/ Hovasse RI cut hSPT4 phage clone #5 in SP72/RI/CIP (clone derived from unmethylated human adult muscle lambda gt10 cDNA library) HB101..... pGH86 ampR hSPT4 fragment
FB1476 pGH87 Hartzog/ Hovasse RI cut hSPT4 phage clone #6 in SP72/RI/CIP (clone derived fron unmethylate d human adult muscle lambda gt10 cDNA library) HB101..... pGH87 ampR hSPT4 fragment
FB1477 pGH88 Hartzog/ Hovasse RI cut hSPT4 phage clone #8 in SP72/RI/CIP (clone derived from unmethylated human adult muscle lambda gt10 cDNA library) HB101..... pGH88 ampR hSPT4 fragment
FB1478 pGH89 Hartzog/ Hovasse RI cut hSPT4 phage clone #9 in SP72/RI/CIP (clone derived from unmethylate d human adult muscle lambda gt10 cDNA library) HB101..... pGH89 ampR hSPT4 fragment
FB1479 pWJ487 J. Smith vector= pUC based with URA3 inserted ***SEE SHEETS ENCLOSED WITH FB1459*** GM48...... dam- pWJ487 MET14 URA3 ampR
FB1480 pWJ488 J. Smith pWJ487 based plasmid (FB1479) with a deletion of MET14 coding seq (D HpaI-Sa lI) GM48...... dam- pWJ488 met14D(HpaI-SalI) URA3 ampR
FB1481 pGH43 Hartzog SPT5 in pRS406 to integrate: use NheI MH1....... pGH43 URA3 ampR SPT5 flori
FB1482 pBM2815 Riles / Johnston see enclosed sheets HB101..... pBM2815 HIS3 ampR
FB1483 FAT-RS303b Gottschling see enclosed sheets MC1066.... FAT-RS303`b' ampR HIS3 2micron ARS leu2-d
FB1484 pGEX-KG David McNabb "REF: Guan and Dixon, Anal Biochem 192:202-267 (1991) " HB101..... pGEX-KG GSTpromoter ampR
FB1485 pGH95 Hartzog / Hovasse RI-XhoI frag from pGH77 (FB1463) containing hSPT4 inserted into RI/XhoI cut pTAG DH10/P3... tetR pGH95 hSPT4 SupF ampR tetR
FB1486 pGH96 Hovasse / Hartzog RI-XhoI frag from pGH78 (FB1464) containing SPT4 into RI/XhoI cut pTAG DH10/P3... tetR pGH96 SPT4 SupF ampR tetR
FB1487 pAD1 A. Dudley 1.5kb SacI-ClaI frag from pLG39 (FB1350) containing his4-912dD44-lacZ subclo ned into 4.2kb SacI/ClaI of pRS304 HB101.3... pAD1 TRP1 ampR his4-912dD44-lacZ
FB1488 pAD11 A. Dudley PacI-BseRI frag (320bp) from site directed mutagenesis plasmid sucloned into PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific mutati on seq. HB101.3... pAD11 his4-912dD44-lacZ-pAD11 TRP1 ampR
FB1489 pAD12 A. Dudley PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq. HB101.3... pAD12 his4-192dD44-lacZ-pAD12 TRP1 ampR
FB1490 pAD13 A. Dudley PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487). See enclosed sheets for specific mut ant seq. HB101.3... pAD13 his4-912dD44-lacZ-pAD13 TRP1 ampR
FB1491 pAD14 A. Dudley PacI-BseRI fragment (320bp) from site directed mutagenesis plasmid subcloned into PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific m utant seq. HB101.3... pAD14 his4-912dD44-lacZ-pAD14 TRP1 ampR
FB1492 pAD15 A. Dudley PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific mutan t seq. HB101.3... pAD15 his4-912dD44-lacZ-pAD15 TRP1 ampR
FB1493 pAD16 A. Dudley PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq. HB101.3... pAD16 his4-912dD44-lacZ-pAD16 TRP1 ampR
FB1494 pAD17 A. Dudley PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq. HB101.3... pAD17 his4-912dD44-lacZ-pAD17 TRP1 ampR
FB1495 pAD18 A. Dudley PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq. HB101.3... pAD18 his4-912dD44-lacZ-pAD18 TRP1 ampR
FB1496 pAD21 A. Dudley PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq. HB101.3... pAD21 his4-912dD44-lacZ-pAD21 TRP1 ampR
FB1497 5309 Lindquist Lab see enclosed sheet. BSJ-72.... pYABU11 ampR hsp104::URA3 lacZ
FB1498 5310 Lindquist Lab see enclosed sheet BSJ-72.... pYABL5 ampR hsp104::LEU2 lacZ
FB1499 pt32... Carlson REF: MCB 13: 3872 (1993) fig.1 unknown... C1 msn2D3::HIS3 ampR
FB1500 pZfh45D2.. Carlson DO NOT USE. M.CARLSON INFORMED US ON JULY 96 THAT THIS CONSTRUCT IS WRONG. REF: MCB 13:3872 (1993) FIG.4. unknown... D7 msn4D2::URA3 ampR
FB1501 pEB312D1.. Carlson REF: NAR 18:6959 (1990) unknown... I2 msn1::URA3 ampR
FB1502 pJH104 Carlson REF: MCB 14:1972 (1994) fig.1 unknown... G9 msn3D2::HIS3 ampR
FB1503 pGH31 Hartzog KpnI-XhoI frag from pGH32 into pGH13. NOTE: this clone complements for func tion in GHY2 and produces protein recognized by 9E10 Ab. MH1.own... pGH31 myc-SPT5 ampR TRP1 CEN
FB1504 pDE82 David Eide for MSN1 disruption REF: J. Gen. Microb. 138: 347-354 (1992) DH5alpha.. pDE82 msn1::URA3 ampR
FB1505 BMB304 Magasanik Lab Sau3A partial of yeast DNA cloned into BamHI site of YEp13 REF: MCB vol. 11 :822 (feb 91) Coschigano+Magasanik HB101.ha.. p1 URE2 ampR LEU2 2micron
FB1506 BMB319 Magasanik Lab URE2 in YCp50 REF: MCB vol.11:822 (feb91) HB101.ha.. p1C-CS URE2 ampR URA3 CEN ARS
FB1507 BMB347 Magasanik Lab REF: MCB vol.11:822 (feb91) XL1.1.ha.. p1C-CSDH0 ure2D12::URA3 ampR CEN ARS
FB1508 pMu-1 M. Schmitt 2kb LEU2 frag into PstI-XbaI sites of STD1 gene in vector YEp352 REF: MCB v ol.13:3650 HB101.ha.. pMu-1 std1D::LEU2 ampR URA3
FB1509 RP28-lacZ Woolford Lab 90bp RI-XbaI frag of RP28 (ric`bosomal protein 28) cloned into YEp357 **Se e sheet in notebook!** plasmid could contain RP28A or PR28B! unknown... RP28-lacZ RP28-lacZ ampR
FB1510 pEY32H Carlson REF: MCB 13:3872 (1993) fig.1 unknown... pEY32H MSN2 ampR 2micron URA3
FB1511 pEL45 Carlson REF: MCB 13:3872 (1993) fig. 4 unknown... pEL45 MSN4 ampR 2micron URA3
FB1512 pJH68 Carlson REF: MCB 14:1972 (1994) fig. 1 unknown... pJH68 MSN3 2micron ampR URA3
FB1513 H7.5 Carlson REF: MCB 14: 1972 (1994) page 1973 unknown... H7.5 MTH1 2micron URA3 ampR
FB1514 pLG9 Gansheroff vector= pET-3c insert= frag of SPT7 from ATG to 1st NdeI site in coding re gion HB101.n... pLG9 pET3c + SPT7 frag under T7 promoter ampR
FB1515 pLG10 Gansheroff vector= pET-3c insert= NcoI frag containing SPT7 from pFW127 HB101.n... pLG10 T7promoter- SPT7 ampR

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