Winston Lab Strain Finder
Department of Genetics

Bacteria


Displaying 501 - 1000 of 2807
Contains
Strain Name Strain Alias Source Comments Host Host Markers Plasmids Plasmid Markers
FB380 Scott Emr for lacZ fusions FB383..... pSEYC58 amp cen4 ars1 lacZ
FB381 FB381 Scott Emr for lacZ fusions FB383..... pSEY18 amp 2 micron lacZ ura3
FB382 Scott Emr for lacZ fusions FB383..... pSEYC68 cen4 ars1 ura3 lacZ amp
FB383 SE10 Scott Emr published in an article in JCB SE10...... none
FB384 Kate partial SPT7 activity MC1061.... pFW116 amp ura SPT7 ClaI-SalI
FB385 Kate no SPT7 activity MC1061.... pFW117 YCp50 SPT7 SphI-RI from pFW110
FB386 Kate no SPT7 activity MC1061.... pFW118 YIp5 SPT7 SphI-RI from pFW110
FB387 Kate no SPT7 activity MC1061.... pFW119 YCp50 SPT7 SphI-Ri
FB388 Kate has SPT8 activity MC1061.... pFW120 YCp50 SPT8 ClaI-SphI
FB389 Kate has SPT8 activity MC1061.... pFW121 YIp5 SPT8 ClaI-SphI
FB390 Kate 2 kb SPT7 fragment MC1061.... pFW122 pBR322 SPT7 Cla-Sal
FB391 Kate see FB381 HB101..... pSEY18 lacZ amp ura3 2 micron
FB392 Scott Emr see FB382 HB101..... pSEYC68 cen4 ars1 ura3 lacZ amp
FB393 Kate 3 kb fragment of SPT7 MC1061.... pFW123 YCp50 SPT7 Sal-RI
FB394 Kate MC1061.... pFW124 YIp5 SPT7 Sal-RI
FB395 Kate y MC1061.... pFW125 YCp50 SPT7 Sal fragment
FB396 Jef Boeke subclone from P2-L4 into Cla site of pBR325 FB1.61.... pJEF705 LYS2 pBR325
FB397 Jan Fassler deletion of Xho-Hpa in pSL42-2 MC1061.... pJF210 lys2del ura3 amp
FB398 Jan Fassler Xho-Hpa deletion in LYS2; same as pJF210 MC1061.... pJF211 lys2del amp ura3
FB399 Kate Cla-RI clone in YCp50; has function MC1061.... pFW127 SPT7 YCp50
FB400 Kate SPT7 Cla-RI clone in YIp5 MC1061.... pFW128 SPT7 YIp5
FB401 Himmelfarb and Friesen dominant suppressor of rpb2-ts mutation .......... pJH143 amp ura3 SRP1
FB402 Himmelfarb and Friesen dominant suppressor of rpb2-ts .......... pJH157 amp ura3 SRP1
FB403 Himmelfarb and Friesen wild type srp1 .......... pJH153 amp ura srp1+
FB404 Kate no SPT7 function MC1061.... pFW129 YCp50 SPT7 ClaI-BglII
FB405 BD817 Bruce Duncan "Gene 28, 211-219 (1984) " BD817..... ung1 nadB7 recA56 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 ga lT22 rpsL relA1(?) none
FB406 BD1528 Bruce Duncan BD1528.... ung1 nadB30 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 galT22 r psL relA1(?) none
FB407 Kate HB101..... pFW130 YCp50 SPT8 ClaI-XhoI
FB408 Kate "RI-SalI fragment second from top of pFW120, around 2.7 kb " HB101..... pFW131 YCp50 SPT8 RI-SalI
FB409 Kate may be switched with FB410 -- BEWARE!!! MC1061.... pFW133 YEp24 SPT8 Sph-Sal
FB410 Kate may be switched with FB409 -- BEWARE!!! MC1061.... pFW132 YCp50 SPT8 Sph-Sal
FB411 Kate 4.5 kb insert MC1061.... pFW134 pBR322 SPT8 ClaI-SphI
FB412 Kate no SPT8 activity MC1061.... pFW135 pBR322 SPT8 RI-SalI
FB413 NK5012 Nancy Kleckner strain Q1 from Ethan Signer NK5012.... C600 TL- B1+ lac- suII T1R isR phi80R none
FB414 NK7147 Nancy Kleckner spontaneous lambdaR from NK5360 NK7147.... "gal1,2 su- strA547 lacZ- r-m+ delhis rec+ lambdaR none
FB415 NK5830 Nancy Kleckner FB413-FB415 are for lambda1224 (Tn10LUK hops) NK5830.... F'lacproiQc8/arg dellacproX111 nalA rifR recA su0 ara none
FB416 Jodi Hirschman His+ derivative of YCp701 (see FB282) HB101..... R255 YCp701 His+ derivative
FB417 Jodi Hirschman HB101..... YCp701-R257 YCp701 His+ derivative
FB418 Jodi Hirschman HB101..... YCp701-R372 YCp701 His+
FB419 Jodi Hirschman HB101..... YCp701-R383 YCp701 His+
FB420 Debby Chaleff see letter in notebook; STE7 probe HB101..... pSTE7.3 amp STE7
FB421 Debby Chaleff STE11 probe; see letter in notebook HB101..... pSTE11.3 amp STE11
FB422 Stan Fields 5 kb insert in YIp5; STE12 probe HB101..... SI14-12 STE12 amp
FB423 Nancy Kleckner for Tn10LUK hops NK5830.... see FB415 pNK629 tet ptac-Tn10 transposase
FB424 Kate BEWARE!!! Not necessarily correct HB101..... pFW136 YCp50 SPT8 Sph-Sal
FB425 N. Kleckner for Tn10LUK hops into SPT7 NK5830.... see FB415 pFW127 and pNK629 see notebook
FB426 N. Kleckner duplicate with FB425 NK5830.... see FB415 "pFW127, pNK629 see notebook
FB427 Kate SPT8 plasmid my have dimers NK5830.... pNK629 and pFW132 see notebook
FB428 Kate beware of dimers for SPT8 plasmid NK5830.... see FB415 pNK629 and pFW132 see notebook
FB429 Kate no SPT8 activity HB101..... pFW137 YCp50 SPT8 Xho-Sal
FB430 Kate no SPT8 activity HB101..... pFW138 YCp50 SPT8 Xho-Sal
FB431 Kate HB101..... pFW139 YCp50 SPT8 Sph-RI
FB432 NK5772 Nancy Kleckner See notebook about checking markers by 2-aminopurine test. NK5772.... dcm-6 dam-3 metB1 galK2 galT22 lacY1 tsx-78 (thi-1 tonA32 mtl-1)? prob ably an amber suppressor str
FB433 Kate HB101..... pFW140 YCp50 SPT8 XhoI-SphI
FB434 Kate HB101..... pFW141 YCp50 spt7-301::Tn10LUK
FB435 Kate y HB101..... pFW142 YCp50 spt7-302::Tn10LUK
FB436 Kate partial SPT7 function HB101..... pFW143 YCp50 spt7-303::Tn10LUK
FB437 Kate no function HB101..... pFW144 YCp50 spt7-304::Tn10LUK
FB438 Kate no function HB101..... pFW145 YCp50 spt7-305::Tn10LUK
FB439 DO NOT USE .B101.....
FB440 DO NOT USE .B101.....
FB441 Kate y HB101..... pFW146 YCp50 spt7-306::Tn10LUK
FB442 Kate HB101..... pFW147 YCp50 spt7-307::Tn10LUK
FB443 Kate HB101..... pFW148 YCp50 spt7-308::Tn10LUK
FB444 Kate HB101..... pFW149 YCp50 spt7-309::Tn10LUK
FB445 Kate HB101..... pFW150 YCp50 spt7-310::Tn10LUK
FB446 Kate y HB101..... pFW151 YCp50 spt7-311::Tn10LUK
FB447 Kate HB101..... pFW152 YCp50 spt7-312::Tn10LUK
FB448 Kate Subclone from pFW127 into YCp50 HB101..... pFW153 YCp50 SPT7 BamHI-EcoRI fragment
FB449 Kate insert is around 1.7 kb HB101..... pFW154 YIp5 SPT7 SalI-BglII
FB450 Kate HB101..... pFW155 pBR322 SPT7 SalI-BglII
FB451 Jef Boeke TRP1 CEN plasmid for subcloning; see notebook HB101..... pBM453 TRP1 ARS1 CEN3 ampR tetR
FB452 Kate HB101..... pFW156 YCp50 spt7-313::Tn10LUK
FB453 Kate HB101..... pFW157 YCp50 spt7-314::Tn10LUK
FB454 Kate HB101..... pFW158 YCp50 spt7-315::Tn10LUK
FB455 Kate HB101..... pFW159 YCp50 spt7-318::Tn10LUK
FB456 Kate HB101..... pFW160 YCp50 spt7-317::Tn10LUK
FB457 Kate HB101..... pFW161 YCp50 spt7-318::Tn10LUK
FB458 Kate HB101..... pFW162 YCp50 spt7-319::Tn10LUK
FB459 Kate HB101..... pFW163 YCp50 spt7-319::Tn10LUK
FB460 Kate HB101..... pFW164 YCp50 spt7-320::Tn10LUK
FB461 Kate HB101..... pFW165 pFW165 YCp50 spt7-321::Tn10LUK
FB462 Kate HB101..... pFW166 YCp50 spt7-322::Tn10LUK
FB463 Kate HB101..... pFW167 YCp50 spt7-323::Tn10LUK
FB464 Kate HB101..... pFW168 YCp50 spt7-324::Tn10LUK
FB465 Kate HB101..... pFW169 YCp50 spt7-325::Tn10LUK
FB466 Jan Fassler Autonomous plasmid with lys2-61; the Ty61 is marked by a lacZ fragment MC1061.... pJF119 Ty61 URA3 ars1 amp cen3 lacZ fragment
FB467 Jan Fassler Same as pJF119 (FB466) but a different lacZ fragment MC1061.... pJF127 amp ura3 Ty61 LYS2 cen3 ars1 lacZ fragment
FB468 Kate Internal SPT8 fragment HB101..... pFW170 YIp5 SPT8 RI-XhoI
FB469 Kate Internal SPT8 fragment in pBR322 HB101..... pFW171 pBR322 SPT8 XhoI-RI
FB470 Kate SPT7 internal fragment in LEU2 integrating vector HB101..... pFW172 YIp33 SPT7 SalI-BglII
FB471 Kate for Tn10LUK hopping into SPT8 FB415..... pNK629 pFW120 "tet and amp, SPT8 Tn10 transposase
FB472 Lenny Guarente See notebook; can be used for UAS testing HB101..... pLG669-Z CYC1-lacZ
FB473 Lenny Guarente TATA substitution vector; see notebook HB101..... pLGSD5del-178to+3 amp URA3 CYC1 TATA deletion
FB474 Kate FB415..... pNK629 pFW120
FB475 CAG10153 Carol Gross This is a nonsense rpoH allele. Grow the strain at 30 or 35. Ts best seen at 42. See notebook for details. CAG10153.. MC1061 rpoH165 zhf50::Tn10 none
FB476 CAG1578 Carol Gross "Ts rpoD allele with linked Tn10. 25-30 is permissive, 42 is nonpermissive. Seenotebook for details. " CAG1578... MC1061 rpoD800 Tn10 none
FB477 David Norris and MA Osley see notebook; hta1-htb1 deletion HB101.8... pdeltrt1 pUC13 trt1 deletion
FB478 D Norris and MA Osley see notebook HB101.8... pdeltrt2 trt2 (hta2-htb2) deletion
FB479 M. Smith .th/U.Va. "from Mitchell Smith, see notebook " HB101..... pMS191 "histones H3 and H4, copy 1, HHT1 HHF1
FB480 Mitchell Smith see notebook HB101..... pMS201 "HHT1, histone H3
FB481 Mitchell Smith see notebook HB101..... pMS202 "HHF1, histone H4
FB482 "D. Norris, M.A. Osley autonomous due to ars near trt1 HB101..... pdeltrt1-URA3 del(hta1-htb1::URA3) in pUC18
FB483 M.A. Osley HB101..... YCp50 TRT1 YCp50 HTA1-HTB1
FB484 "D.Norris, M.A. Osley HB101..... YEp24-TRT1 YEp24 HTA1-HTB1
FB485 .B101.....
FB486 Kate FB415..... pFW173 YCp50 spt8-301::Tn10LUK
FB487 Kate HB101..... pFW174 YCp50 spt8-302::Tn10LUK
FB488 Kate FB415..... pFW175 YCp50 spt8-303::Tn10LUK
FB489 Kate FB415..... pFW176 YCp50 spt8-304::Tn10LUK
FB490 Kate FB415..... pFW177 YCp50 spt8-305::Tn10LUK
FB491 Kate FB415..... pFW178 YCp50 spt8-306::Tn10LUK
FB492 Kate FB415..... pFW179 YCp50 spt8-307::Tn10LUK
FB493 Kate FB415..... pFW180 YCp50 spt8-308::Tn10LUK
FB494 Kate FB415..... pFW181 YCp50 spt8-309::Tn10LUK
FB496 Kate FB415..... pFW183 YCp50 spt8-311::Tn10LUK
FB497 Kate FB415..... pFW184 YCp50 spt8-312::Tn10LUK
FB498 Kate FB415..... pFW185 YCp50 spt8-313::Tn10LUK
FB499 Kate FB415..... pFW186 YCp50 spt8-314::Tn10LUK
FB500 Kate FB415..... pFW187 YCp50 spt8-315::Tn10LUK
FB501 Kate FB415..... pFW188 YCp50 spt8-316::Tn10LUK
FB502 Tom Stevens for PEP4 disruptions MC1061.... pTS15 pep4del::URA3 pBR322
FB503 Tom Stevens for PEP4 disruption MC1061.... pTS17 pep4del::LEU2 pBR322
FB504 Kate HB101..... pFW189 pGEM1 SPT7 Sal-BglII
FB505 Kate HB101..... pFW190 pGEM2 SPT7 SalI-BglII
FB506 Elizabeth Craig 700 bp Rsa fragment of HSP70 for Northern probe HB101..... YG106-RSA HSP70 Rsa fragment pUC9
FB507 LE392 Leder lab originally made by Lynn Enquist LE392..... F- hsdR514 (r-K m-K) supE44 supF58 lacY1 or del(lacIZY)6 galK2 galT22 metB1 trpR55 lambda- none
FB508 Kate HB101..... pFW191 pCGS42 SPT7 Cla-RI
FB509 Kate HB101..... pFW192 pCGS42 SPT7 ClaI-SalI
FB510 Kate HB101..... pFW193 pCGS42 SPT8 ClaI-SphI
FB511 Anne Happel "H3-Bam fragment, no activity " HB101..... pRSP4-27 rsp4-27 pBR322
FB512 Jan Fassler "subclone from pJF203 into pGEM1, 1.9 kb fragment " HB101..... pJF309 pGEM1 SPT14 XbaI-G insert
FB513 Jan Fassler subclone from pJF235 HB101..... pJF314 pUC19 SPT13 Sph-Sal
FB514 Jan Fassler HB101..... pJF62 pUC18 SPT4 RI=RI
FB515 Christy y HB101..... pCC39 pBR322 SPT6 BglII fragment
FB516 Christy HB101..... pBR322 SPT16 BamHI "4.36 BamHI fragment class I""
FB517 K. Struhl KC8.1..... leuB600 trpC983 hisB463 pyrF74::Tn5 dellacX74 strA galUK none
FB518 David Norris 6.4 kb Bam fragment HB101..... YIp5-TRT1 YIp5 HTA1-HTB1 BamHI
FB519 David Norris 3.55 insert fragment HB101..... YIp5-TRT2 YIp5 HTA2-HTB2 BamHI-EcoRI
FB520 NK8018 Nancy Kleckner NK8018.... malB (lambda R) leu2 trp31 his1 argG str64 tonA tsx supE44 xyl7 ntl2 p ro+ lacdelr1 (z-y+) metb none
FB521 Gaber and Culbertson 0.55 kb insert .K8018.... RG2-2 YIp5 suf16+ HindIII-SalI
FB522 Phil Hieter see notebook; for generating chromosome fragmentation HB101..... p299 = YCF3 URA3 SUP11
FB523 Phil Hieter see notebook; for chromosome fragmentation HB101..... p300 = YCF4 URA3 SUP11
FB524 Rob Jensen via Paul Sternberg; see notebook HB101..... pRJ86 HO-lacZ
FB525 Linda Breeden via Paul Sternberg; see notebook HB101..... pLB46 HO-lacZ
FB526 Dave Hill do not give out HB101..... pAJ724 expression vector with APase promoter
FB527 Dave Hill do not give out HB101..... pAB701 GPDH vector
FB528 Dave Hill do not give out .B101..... pS220 GPDH vector
FB529 Culbertson .B101..... pRG15-1 YIp5 SUF16-3
FB530 Culbertson .B101..... pRG17-1 YIp5 SUF16
FB531 Culbertson .B101..... pRG28 YIp5 SUF16-4
FB532 CY82 George Sprague Cell 32: 409 RR1.1..... pSC7 YIp5 STE3
FB533 CY786 George Sprague RR1.1..... pAB510 pBR322 STE2
FB534 Jules O'Rear Plasmid is probably WRONG. DH1....... pFW194 spt3-202::URA3-2 amp URA3
FB535 Jules O'Rear "BEWARE, plasmid may not be correct " DH1....... pFW195 spt3-202::URA3-10 amp URA3
FB536 C. Clark . 6kb SPT6 BglII fragment cloned into BamHI site of YEP24 HB101..... pCC14 SPT6 (BglII frag) 2micron URA3 ampR
FB537 Pfeifer and Guarente "Cell 49: 19-27, see notebook " HB101..... pSP64AX CYC1 UAS1 amp
FB538 Kate HB101..... pFW196 pBR322 SPT7 Cla-RI (ampR tetR)
FB539 Lenny Guarente see notebook and FB473 HB101..... pLGSD5delATG pLGSD5 with ATG deleted
FB540 Lenny Guarente see notebook; see FB472 HB101..... pLG669ZdelATG see notebook
FB541 B89 G.R. Fink see map in notebook .B101..... pHIS4(Pst) pBR322 HIS4 Pst fragment
FB542 R. Gaber see FB521 HB101..... pRG2-2 YIp5 suf16+
FB543 R. Gaber see FB529 HB101..... pRG15-1 YIp5 SUF16-3
FB544 R. Gaber see FB530 HB101..... pRG17-1 YIp5 SUF16-2
FB545 R. Gaber see FB531 HB101..... pRG28 YIp5 SUF16-4
FB546 Jodi Fragment is from Wei Chen plasmid pUC8 RI-Sal 2605 HB101..... pJEH122 pUC18 DED1 Bam-Pst
FB547 Jodi PET56 probe HB101..... pJEH123 pUC18 PET56 Bam-Pst
FB548 Jodi HIS3 probe; 0.7 kb mRNA HB101..... pJEH124 pUC18 HIS3 Bam-Pst
FB549 Fred "clone #1, Sau3A partial " HB101..... pFW197 YCp50 RSP5
FB550 Fred clone #7 HB101..... pFW198 YCp50 RSP5
FB551 Jan see map in notebook HB101..... pJF42 YCp50 SPT4 RI-RI
FB552 Jan HB101..... pJF74
FB553 Jan HB101..... pJF75
FB554 Jan "An spt4 null; deletion made from a PvuII in Tn5 to the SPT4 PvuII; then, URA 3 inserted next door at Xho. See notbook. " FB1.1..... pJF104 pRG331 spt4::Tn5delPvuIIURA3
FB555 Jan MC1061.... pJF178
FB556 Jan Fassler MC1061.... pJF179
FB557 Jan Fassler .C1061.... pJF182
FB558 Jan Fassler MC1061.... pJF195
FB559 Jan Fassler MC1061.... pJF203
FB560 Jan Fassler TB1.61.... pJF212 pSEY668 Sal-HIII for Bal31-1.8; pJF172 #1.1
FB561 Jan Fassler TB1.61.... pJF213 pSEY668 Sal-HIII for Bal20-5 pJF195 #4.1
FB562 Jan Fassler TB1.61.... pJF214 pSEY668 Sal-HIII for Bal2.18 pJF179 #5.2
FB563 Jan Fassler TB1.61.... pJF215 pSEY668 Sal-HIII for Bal3.7 pJF178 #6.1
FB564 Jan Fassler TB1.61.... pJF216 pSEY668 Sal-HIII for Bal10.1 pJF182 #7.2
FB565 Jan Fassler TB1.61.... pJF235 pSEYC68 SPT13 Sph fragment
FB566 Jan Fassler NK5830.... pJF243 pJF239 spt13::Tn10-LUK #2.2
FB567 Jan Fassler NK5830.... pJF272 pJF203-3 spt14::Tn10LUK#D2
FB568 Jan Fassler MC1061.... pJF328 see notebook
FB569 Jan Fassler see notebook MC1061.... pJF347 see notebook
FB570 Jan Fassler see notebook MC1061.... pJF348 see notebook
FB571 Jan Fassler see notebook MC1061.... pJF349 see notebook
FB572 Jan Fassler see notebook MC1061.... pJF377 see notebook
FB573 Jan Fassler see notebook MC1061.... pJF110 YCp50 Ty61delSal-LYS2
FB574 Jan Fassler see notebook MC1061.... pJF172 Bal31 #1.8a
FB575 Jan Fassler see notebook MC1061.... pJF376 see notebook
FB576 Fred "H3-SphI insert, around 6 kb " .......... pFW199 pBR322 RSP5
FB577 Fred "H3-SphI insert, around 6 kb " .......... pFW200 YIp5 RSP5
FB578 Fred "H3-SphI insert, around 6 kb " .......... pFW201 YCp50 RSP5
FB579 "H3-SphI insert, around 6 kb " .......... pFW202 pCGS42 RSP5
FB580 Christy "see PNAS 77, 3912-3916 Ng and Abelson " HB101..... pCC69 pBR322 ACT1 Bam-H3 around 1.6 kb insert
FB581 Rick Young functional clone of RPB1 HB101..... pRP112 YCp50 RPB1
FB582 Rick Young Functional subclone of RPB2 HB101..... pRP212 YCp50 RPB2
FB583 Pfieffer and Guarente see notebook TB!.1..... pSP64-CY CYC7 UAS - lacZ
FB584 Mary Ann Osley see notebook HB101..... pUC13-del16b HTA1-HTB1 with ARS deletion
FB585 Mary Ann Osley see notebook HB101..... pUC13-del16bURA3 HTA1-HTB1 with ARS deletion marked with URA3
FB586 "Daria Hekmatpanah, Fink 1.1 kb insert HB101..... pDH22 pUC18 HIS4 SalI-PvuII into SalI-SmaI
FB587 "Daria Hekmatpenah, Fink 1.45 kb insert derived from B701 HB101..... pDH23 pDH23 pCU18 his4-912delta SalI-PvuII into SalI-SmaI
FB588 "Daria Hekmatpenah, Fink 1.65 kb insert from pRB153 (Botstein) HB101..... pDH25 pUC18 ACT1 BamHI-HindIII into same
FB589 Kate HB101..... pFW203 pBR322 SPT7 Cla-RI
FB590 Forrest Chumley and G.R. Fink chromatin probe for HIS4 FB1.1..... pFC1 pBR322 HIS4 RI-Sal 249 bp fragment
FB591 Jodi Rsa-Taq from YCp701 into Sma-AccI of mp19 then into Ri-HindIII of pBR322 HB101..... pJEH6 pBR322 his4-912delta 470 bp fragment
FB592 Roger Brent "see notebook and West et al MCB 4, 2467 " .B101..... LRdel20B GAL1-lacZ with Xho site between UAS and TATA
FB593 D. Ruden and M. Ptashne HB101..... pRY131 GAL; see notebook
FB594 D. Ruden and M. Ptashne HB101..... LR1del23 GAL; see notebook
FB595 D. Ruden and M. Ptashne HB101..... LR1del24 GAL; see notebook
FB596 D. Ruden and M. Ptashne HB101..... "LR1del(25-25,23) GAL; see notebook
FB597 D. Ruden and M. Ptashne HB101..... "LR1del(25,25,24) GAL; see notebook
FB598 D. Ruden and M. Ptashne HB101..... pRY171 GAL; see notebook
FB599 Kate derived from pFW127 TB1.1..... pFW204 pUC19 SPT7 Sal-Bam
FB600 MC1065 "Casadaban, Struhl, Raymond "For scoring trp, use drop out media. Also see FB326. " MC1065*... "X1486 M+h12 leuB600 trpC9830 pyrF::Tn5 hisB463 dellacX74 strA galU,K none
FB601 Charlie see notebook HB101.*... pCHY10 GAL-SPT3 2 micron
FB602 Jef Boeke 3 kb SPT21 Xho fragment in pRS22 HB101..... pGN984 SPT21 CEN4 LEU2
FB603 Jef Boeke SPT22=SPT10; insert is greater than 10 kb; in pRS22 HB101..... pGN990 SPT22 LEU2 CEN4
FB604 Ma and Ptashne see notebook HB101..... pMA424 GAL4 DNA binding domain HIS3 amp 2 micron
FB605 Stan Fields lacZ library; see notebook MC1066.... del(lac)X74 hsr- hsm+ strA trpC9830 leuB600 pyrF::Tn5 galU galK fusion library to lacZ
FB606 Stan Fields lacZ fusion library; see FB605 and notebook MC1066.... lacZ fusion library
FB607 Stan Fields lacZ fusion library; see FB605 and notebook MC1066.... see FB605 lacZ fusion library
FB608 Kupiec and Simchen "see notebook; Current Genetics 8, 559-566 " HB101..... pDEL21 URA3 rad6del
FB609 Kupiec and Simchen see notebook; orientation of fragment unsure HB101..... YH6 YIp5 RAD6
FB610 Kate SPT7 RI-Bam in pBM453 RI-Bam HB101..... pFW205 SPT7 TRP1 CEN amp
FB611 Jodi see FB595 and notebook HB101..... pJEH22 GAL-lacZ with 912delta-derived 68mer covering TATA in LR1del24
FB612 Jodi see notebook HB101..... pJEH23 GAL lacZ 912delta 68mer
FB613 Jodi see notebook HB101..... pJEH24 "LR1del(25-25,24) 912delta 68mer GAL
FB614 Jodi see notebook HB101..... pJEH25 GAL lacZ 912delta 68mer
FB615 Jodi see notebook HB101..... pJEH26 GAL lacZ 912delta 26mer
FB616 Jodi see notebook HB101..... pJEH27 GAL lacZ 912delta 26mer
FB617 Jodi see notebook HB101..... pJEH28 GAL lacZ 912delta
FB618 Jodi see notebook HB101..... pJEH29 GAL lacZ 912delta
FB619 Jodi see notebook HB101..... pJEH30 GAL lacZ 912delta
FB620 Stratagene "Bluescript KS polylinker, see notebook " .B101..... Bluescript m13+ KS polylinker Bluescript
FB621 Stratagene Bluescript .B101..... Bluescript m13+ "SK polylinker, Bluescript
FB622 Stratagene "bluescript, see notebook " .B101..... Bluescript m13- "KS polylinker, bluescript
FB623 Stratagene "bluescript, see notebook " .B101..... Bluescript m13- "SK polylinker, bluescript
FB624 Ruden and Ptashne see FB592 and notebook HB101..... LRadel24T+ GAL lacZ TATA test plasmid
FB625 Ruden and Ptashne see FB592 and notebook HB101..... LR1del24T- GAL lac TATA test plasmid
FB626 JM109 see notebook JM109..... recA1 endA1 gyr96 thi hsdR17(rk- mk+) supE44 relA1 lambda- del(lac pro AB)/ F' traD36 proAB lacIqZd none
FB627 Russell and Nurse "see notebook; Russell and Nurse Cell 45, 145 " HB101..... pEVP11 LUE2 amp S. pombe ADH promoter
FB628 see notebook HB101..... pSS31 S. pombe ade6+ in pDB248
FB629 see notebook HB101..... pYK311 S. pombe leu1+ pBR322
FB630 Paul Russell "contains promoter for S. pombe triose phosphate isomerase, Gene 40, 125 (198 5) " HB101..... pTPIpo1 YEp13 S. pombe TPI promoter
FB631 Biorad via Dana Boyd see Biorad info (not in notebook) .B101..... dut- ung- thi- relA1 pCJ236 camR F' pox
FB632 Antebe and Fink "J. Bact. 166, 313 (1986); subclone of pTLG2 into YIp5; RI-H3 fragment " HB101..... pAA1 YIp5 GAL2
FB633 Mary Ann Osley see notebook HB101..... pCALA-trt2.97 HTA2-lacZ fusion CEN vector
FB634 Kate HB101..... pFW206 pBR322 SPT7 RI-Bam
FB635 Garfinkel and Boeke "see Science 239, 280 and notebook " HB101..... pGtyH3NEO GAL TyH3-NEO
FB636 M. Grunstein "see Cell 45, 445; Cell 35, 711 " .......... pJC102 HTA1 CEN3 URA3 ARS1
FB637 Grunstein "Cell 45, 445 " .......... pTS2 HTA1(d4-20) CEN ARS URA
FB638 Grunstein "Cell 45, 445 " .......... pTS9 HTA1d(124-131) CEN ARS URA3
FB639 Grunstein "Cell 45, 445 " .......... pTS10 HTA1d(4-29) CEN ARS URA3
FB640 Grunstein "Cell 45, 445 " .......... pMH12 HTA1(d114-131) CEN ARS URA3
FB641 Grunstein "Cell 45, 445 " .......... pMH13 HTA1(d30-45) CEN ARS URA3
FB642 Christine see other entry for this plasmid for info RR1....... pATH1 trp
FB643 Christine see other entry of this plasmid for more info RR1....... pATH2 trpE
FB644 Christine see other entry for more info on this plasmid RR1....... pATH3 trpE
FB645 Christine in-frame fusion RR1....... B4 pATH2 SPT7 Bgl2-Bam
FB646 Christine out-of-frame fusion RR1....... D2 pATH1 SPT7 Bgl2-Bam
FB647 Christine in frame fusion RR1....... A pATH3 SPT8 Bam-Bam
FB648 Christine out of frame fusion .R1....... E2 pATH1 SPT8 Bam-Bam
FB649 Christine out of frame fusion .R1....... B3 pATH2 SPT8 Bam-Bam
FB650 Koerner and Tzagaloff see FB333; for trpE fusions HB101..... pATH11 trpE
FB651 MC1000 Dana Boyd requires leu + ile to grow on minimal medium MC1000.... malFdel3 phoA delphe phoR- F' F' lacpro lacIq camR
FB652 Anne Happel for spt3::TRP1 null HB101..... pAH100 spt3-203::TRP1 pCGS42
FB653 Kate for spt7 null HB101..... pFW207 spt7::URA3 amp URA3
FB654 Kate see notebook and Kate's notes HB101..... pFW208 "spt7 acid"" deletion 2b1
FB655 Kate "in YIp5, see notebook " HB101..... pFW209 "spt7 acid"" deletion URA3 amp
FB656 Hieter "Genetics 122, 19-27 " XL Blue... "recA1 endA1 gyrA96 thi hsdRi7 (rk- mk+) supE44 relA1 almbda-/F' proAB lacIQZdelm15 Tn10 (see Biot pRS303 YIp HIS3 Bluescript
FB657 Sikorski and Hieter "Genetics 122, 19-27 " XL Blue... see FB656 pRS313 YCp HIS3 Bluescript
FB658 Sikorski and Hieter "Genetics 122, 19-27 " XL Blue... see FB656 pRS304 YIp TRP1 Bluescript
FB659 Sikorski and Hieter "Genetics 122, 19-27 " XL Blue... see FB656 pRS314 YCp TRP1 Bluescript
FB660 Sikorski and Hieter "Genetics 122, 19-27 " XL Blue... see FB656 pRS305 YIp LEU2 Bluescript
FB661 Sikorski and Hieter "Genetics 122, 19-27 " XL Blue... see FB656 pRS315 YCp LEU2 Bluescript
FB662 Sikorski and Hieter "Genetics 122, 19-27 " XL Blue... see FB656 pRS306 YIp URA3 Bluescript
FB663 Sikorski and Hieter "Genetics 122, 19-27 " XL Blue... see FB656 pRS316 YCp URA3 Bluescript
FB664 T. St. John via K. Struhl see notebook; GAL probe HB101.e... pUC8 GAL UAS 4812
FB665 T. St. John via K. Struhl "see notebook, GAL probe " HB101.e... pUC8 GAL fragment 4902
FB666 Hieter for transplacement HB101..... p13 YRp14 trp1del1
FB667 Hieter for leu2 transplacement; see notebook .B101..... p307 YRp15 leu2del1
FB668 Hieter for trp1 transplacement; see notebook XL1-13.... p430 YRp14 trp1del63
FB669 M. Fasullo via P. Hieter for his3 transplacement RR1.13.... p510 YRp14 his3del200
FB670 T. Som and J. Broach see notebook; Northern probe HB101..... pYST138 TUB2
FB671 T. Som and J. Broach see notebook HB101..... pYST121 ACT1
FB672 Alani and Kleckner "Genetics 117, 5-12 " HB101..... pNKY54 HIS4-URA3 TRP1
FB673 D. Hekmatpenah HIS4 probe; see notebook HB101..... pDH45 HIS4
FB674 Kassir and Simchen "Cell 52, 853; also see FB786 " HB101..... YEpK26 YEp24 IME1
FB675 Kate Initial SPT10 clone from YCp50 library HB101..... pFW210 YCp50 SPT10
FB676 Kate original SPT10 clone spt10.15 HB101..... pFW211 SPT10 YCp50
FB677 Kate clone spt10.16 HB101..... pFW212 YCp50 SPT10
FB678 Kate original SPT15 clone 15.1 HB101..... pFW213 YCp50 SPT15
FB679 Kate original SPT15 clone 15.3 HB101..... pFW214 YCp50 SPT15
FB680 Kate original SPT15 clone 15.6 HB101..... pFW215 YCp50 SPT15
FB681 Kate y TB1.1..... pFW216 pRS306 SPT10 Sal-H3
FB682 Kate TB1.1..... pFW217 pRS316 SPT10 Sal-H3
FB683 Kate SPT15 fragment is around 4.8 kb TB1.1..... pFW218 pRS306 SPT15 Cla-Xho
FB684 Kate TB1.1..... pFW219 pRS316 SPT15 Cla-Xho
FB685 Carl Falco "Genetics 109, 21-35 (1985) " HB101..... pKD1-2 ILV2 YIp5
FB686 Carl Falco "Genetics 109, 21-35 " HB101..... pKD3-4 YIp5 ilv2deletion
FB687 Carl Falco see FB685 HB101..... pBT177 ILV2 2 micron URA3
FB688 Kate for Tn10LUK hops into SPT10 FB413..... pNK629 pFW217
FB689 Kate for Tn10LUK hops into SPT15 FB413..... pNK629 pFW219
FB690 Kate from SPT15.1 clone HB101..... pFW220 pRS316 SPT15 Bam-Cla
FB691 Kate HB101..... pFW221 pBR322 SPT15 Bam-Cla
FB692 Kate FB413..... pNK629 pFW213
FB693 Kate FB415..... pFW222 pFW217 spt10::Tn10LUK
FB694 Kate FB415..... pFW223 pFW217 spt10-301::Tn10LUK
FB695 Kate FB415..... pFW224 pFW217 spt10-302::Tn10LUK
FB696 Kate FB415..... pFW225 pFW217 spt10-303::Tn10LUK
FB697 Kate FB415..... pFW226 pFW217 spt10-304::Tn10LUK
FB698 Kate FB415..... pFW227 pFW217 spt10-305::Tn10LUK
FB699 Kate FB415..... pFW228 pFW217 spt10-306::Tn10LUK
FB700 Kate FB415..... pFW229 pFW217 spt10-307::Tn10LUK
FB701 Kate FB415..... pFW230 pFW217 spt10-308::Tn10LUK
FB702 Kate FB415..... pFW231 pFW217 spt10-309::Tn10LUK
FB703 Kate FB415..... pFW232 pFW217 spt10-310::Tn10LUK
FB704 Kate FB415..... pFW233 pFW217 spt10-311::Tn10LUK
FB705 Kate FB415..... pFW234 pFW217 spt10-312::Tn10LUK
FB706 Kate FB415..... pFW235 pFW217 spt10-313::Tn10LUK
FB707 Kate FB415..... pFW236 pFW217 spt10-314::Tn10LUK
FB708 Kate FB415..... pFW237 pFW217 spt10-315::Tn10LUK
FB709 Kate FB415..... pFW238 pFW217 spt10-316::Tn10LUK
FB710 Kate FB415..... pFW239 pFW217 spt10-317::Tn10LUK
FB711 Kate FB415..... pFW240 pFW217 spt10-318::Tn10LUK
FB712 Kate FB415..... pFW241 pFW217 spt10-319::Tn10LUK
FB713 Kate FB415..... pFW242 pFW217 spt10-320::Tn10LUK
FB714 M. Carlson "MCB 6, 3647; for snf2 transplacements " HB101..... pAC98 amp snf2::HIS3
FB715 M. Carlson "for transplacement of snf5 null; MCB 6, 3647 " HB101..... pAC186 amp snf5::URA3
FB716 Kate TB1.1..... pFW243 pUC18 SPT10 XbaI fragment from pFW210
FB717 Carol Lusty "Gene 45, 299-310; also see FB717-734; FB798-815 " .......... YEp353 'lacZ
FB718 C. Lusty see FB717 .......... YIp353 'lacZ
FB719 C. Lusty see FB717 .......... YEp354 'lacZ
FB720 C. Lusty see FB717 .......... YIp354 'lacZ
FB721 C. Lusty see FB717 .......... YEp355 'lacZ
FB722 C. Lusty see FB717 .......... YIp355 'lacZ URA3
FB723 C. Lusty see FB717 and notebook .......... YEp356 'lacZ URA3
FB724 C. Lusty see FB717 and notebook .......... YIp356 'lacZ URA3
FB725 C. Lusty see FB717 and notebook .......... YEp357 'lacZ URA3
FB726 C. Lusty see FB717 and notebook .......... YIp357 'lacZ URA3
FB727 C. Lusty see FB717 and notebook .......... YEp358 'lacZ URA3
FB728 C. Lusty see FB717 and notebook .......... YIp358 'lacZ URA3
FB729 C. Lusty see FB717 and notebook .......... YEp356R 'lacZ URA3
FB730 C. Lusty see FB717 and notebook .......... YIp356R 'lacZ URA3
FB731 C. Lusty see FB717 and notebook .......... YEp357R 'lacZ URA3
FB732 C. Lusty see FB717 and notebook .......... YIp357R 'lacZ URA3
FB733 C. Lusty see FB717 and notebook .......... YEp358R 'lacZ URA3
FB734 C. Lusty see FB717 and notebook .......... YIp358R 'lacZ URA3
FB735 B. Daignan-Fornier This strain is for making MuLEU2 hops; see notebook JM109..... JM109 MuCts ZZ4 CAMR
FB736 B. Daignon-Fornier see notebook M8820.....
FB737 Jan TB1.0..... pJF64 pUC18 SPT4 RI-RI
FB738 D. Gallwitz "S. pombe act1 clone; NAR 15, 7369; see notebook " RR9.0..... pSPA2 S. pombe act1
FB739 Celenza and Carlson for making snf1 null; see notebook .R9.0..... pCN106 snf1 deletion
FB740 Celenza and Carlson SNF1 probe; see notebook .R9.0..... pCN8 SNF1
FB741 Celenza and Carlson see FB739 in notebook for information; for making snf4 null .R9.0..... pFE27-32b snf4 deletion
FB742 Celenza and Carlson "SNF4 probe, see FB739 in notebook for more information " .R9.0..... pC8 SNF4
FB743 Greg HB101..... pGP20 YIp5 his4-912d SalI fragment
FB744 Greg opposition orientation from pGP20 HB101..... pGP21 YIp5 his4-912delta
FB745 Wendy Raymond PHO5 promoter JM101..... pNKY323 PHO5-RAD50
FB746 Jan HB101..... pJF27 YEp24 SPT4 Bam-Bam from pJF7
FB747 Charlie pfbp into pFL20. Polylinker at +4 for transcription. See notebook. HB101..... pCHY20 fbp1 promoter URA3 pombe ARS amp
FB748 Charlie polylinker at +272 for transcription HB101..... pCHY21 "fbp1', pombe ars, amp, URA3
FB749 Christine For spt8 null; to integrate cut with Xba + Bam; use smaller fragment; see no tebook HB101..... pCJEC1 spt8d302::LEU2 URA3 amp
FB750 Joel "pUC18; NheI site is created, HindIII site is destroyed " TB1.1..... pJH1 pUC18 amp NheI
FB751 Joel "HindIII destroyed, NheI created " TB1.1..... pJH2 pUC19 NheI
FB752 Lenny Guarente Deletion removes all TATAs and the first transcription initiation site. All other initiation sites remain. HB101..... del-178/+3 CYC1-lacZ URA3 amp
FB753 L. Sarokin and M. Carlson "see notebook; MCB 4, 2750; MCB 7, 672 " HB101..... pLS27 YIp5 suc2 del -1900/-390
FB754 Mary Ann Osley see notebook; CEN plasmid HB101..... pSKA1 HTA1-lacZ amp LEU2
FB755 M. Mcleod; D. Beach Vector used for pombe library; cloning site in lambda cI gene; inserts confe r tet resistance HB101..... pWH5 LEU2 amp 2 micron
FB756 Jan Strain is very sick; large colonies have lost the plasmid. HB101..... pJF64 pUC18 SPT4 RI fragment
FB757 Mark Johnston see notebook HB101..... pB7272 GAL UAS CEN amp URA3 ARS1
FB758 Botstein see notebook .B101..... pRB758 LEU2 polylinker
FB759 Botstein .B101..... pRB759 URA3 polylinker
FB760 Kate fragment is around 2.2 kb HB101..... pFW244 pRS316 SPT7 Cla-Sal
FB761 M.A. Osley Beware - may be wrong - check it out first .B101..... pBL102 CYC1-lacZ
FB762 M.A. Osley Normalization probe for S1; see notebook .B101..... pHS2 RP51A
FB763 Meluh and Rose HB101..... pMR869 pPHO5-TRP1 URA3
FB764 Vallen and Rose HB101..... pMR902 pPHO5-KAR1 in YCp50
FB765 Vallen and Rose see notebook HB101..... pMR886 pPHO5 in YIp5
FB766 L. Prakash overexpresses RAD6 in yeast .......... pSCW242 ADC-RAD6 2 micron TRP1 amp
FB767 L. Prakash "Genes and Dev. 2, 1476 " .......... pR619 ADC-rad6-149 2 micron TRP1 amp
FB768 L. Prakash "MCB 8, 1179 " .......... pR67 YCp50 RAD6
FB769 L. Prakash "MCB8, 1179 " .......... pR615 YCp50 rad6-149
FB770 L. Prakash "MCB 8, 1179 " .......... pR616 YCp50 rad6-153
FB771 L. Prakash "MCB 8, 1179 " .......... pR617 YCp50 rad6-164
FB772 Chris Hardy via Jan "see notebook; from David Shore's lab, probably " HB101..... pGleual1 (?) gal4::LEU2
FB773 C. Denis see notebook HB101..... pC1 CCR4 LEU2
FB774 .B101..... pGC21
FB775 M.A. Osley 20 amino acids of HTB1 left; see notebook HB101..... pSKB1-20 HTB1-lacZ
FB776 M.A. Osley 7 aa of H2B1 fused to lacZ; see FB775 for map HB101..... pSKB1-7 HTB1-lacZ amp
FB777 M.A. Osley Exact structure unknown HB101..... pBL101 H2B2-lacZ
FB778 M.A. Osley 80 aa H2B1-lacZ; see map with FB775 HB101..... pCALB1 HTB1-lacZ amp
FB779 Kate HB101..... pFW245 pCGS42 SPT10 H3-SalI
FB780 Kate Integrating version; CEN deleted HB101..... pFW246 HTB1-lacZ amp
FB781 Kate Integrating version; in HB101 .B101..... pFW247 HTB1-lacZ amp
FB782 .B101.....
FB783 Kate Integrating form; see notebook HB101..... pFW249 HTB1-lacZ amp
FB784 Kate Integrating form; see notebook HB101..... pFW250 HTA2-lacZ
FB785 Kate Integrating form; see notebook HB101..... pFW251 HTA1-lacZ
FB786 Aaron Mitchell see FB674 for other IME1; do not give out; see notebook .......... pHS103 YEp24 IME1
FB787 Clyde Denis see notebook .......... pHH20 ADHI disruption
FB788 Clyde Denis .......... pHH21 ADHI disruption; see notebook
FB789 Kate for in vitro expression HB101..... pFW252 pGEM1 SPT8 SnuBI-SpeI
FB790 V. Zakian for overexpression; see notebook .B101..... YEpFAT10 leu2d
FB791 V. Zakian for overexpression; see notebook .B101..... YEpFAT4 leu2d
FB792 Stan Fields for Stan Fields interaction experiment; see notebook .B101..... pNY7 pUC18 GAL4 activation domain II
FB793 Stan Fields For Stan Fields experiment; see notebook .B101..... pMA424 HIS3 ADH1-GAL4(1-147) amp polylinker
FB794 M. Grunstein see notebook MC1061.... pMH313 PHO5-lacZ
FB795 M. Grunstein "see notebook; Cell 55, 1137; UAS deletion " MC1061.... pMH314 PHO5-lacZ
FB796 M. Grunstein "Cell 55, 1137; PHO5 deletion; see notebook " MC1061.... pMH324 PHO5-lacZ
FB797 M. Grunstein "Cell 55, 1137; PHO5 UAS deletion; see notebook " MC1061.... pMH329 PHO5-lacZ
FB798 Carol Lusty "Gene 45, 299-310; see FB717 for URA3 versions; see notebook; see 798-815 " HB101..... YEp363 lacZ LEU2
FB799 Carol Lusty see FB798 HB101..... YIp363 lacZ LEU2
FB800 Carol Lusty see FB798 and notebook HB101..... YEp364 lacZ LEU2
Fb801 Carol Lusty see notebook and FB798 HB101..... YIp364 lacZ LEU2
FB802 Carol Lusty see notebook and FB798 HB101..... YEp365 lacZ LEU2
FB803 Carol Lusty see notebook and FB798 HB101..... YIp365 lacZ LEU2
FB804 Carol Lusty see notebook and FB798 HB101..... YEp366 lacZ LEU2
FB805 Carol Lusty see notebook and FB798 HB101..... YIp366 lacZ LEU2
FB806 Carol Lusty see notebook and FB798 HB101..... YEp367 lacZ LEU2
FB807 Carol Lusty see notebook and FB798 HB101..... YIp367 lacZ LEU2
FB808 Carol Lusty see notebook and FB798 HB101..... YEp368 lacZ LEU2
FB809 Carol Lusty see notebook and FB798 HB101..... YIp368 lacZ LEU2
FB810 Carol Lusty see notebook and FB798 HB101..... YEp366R lacZ LEU2
FB811 Carol Lusty see notebook and FB798 HB101..... YIp366R lacZ LEU2
FB812 Carol Lusty see notebook and FB798 HB101..... YEp367R lacZ LEU2
FB813 Carol Lusty see notebook and FB798 HB101..... YIp367R lacZ LEU2
FB814 Carol Lusty see notebook and FB798 HB101..... YEp368R lacZ LEU2
FB815 Carol Lusty see notebook and FB798 HB101..... YIp368R lacZ LEU2
FB816 Dave Eisenmann HB101..... pDE28-6 SPT15 pRS316
FB817 Dave high copy number plasmid in yeast HB101..... pDE31-7 SPT15 pCGS42
FB818 Dave HB101..... pDE32-1 pUC118 SPT15 RI-Bam
FB819 Dave "To makenull, isolate RI-Bam fragment (smaller of 2 bands) and transform " HB101..... pDE34-6 YCp50 spt15-100::URA3
FB820 Betsy Alias is hcs1-101::LEU2; see notebook HB101..... pBM10 spt16-101::LEU2
FB821 Betsy TB1.1..... pBM13 pRS314 SPT16 Bam
FB822 Betsy y TB1.1..... pBM15 pRS315 SPT16 Bam
FB823 Betsy HB101..... pBM29 YCp50 SPT4 RI-H3
FB824 Betsy Cen plasmid; bacterial strain is very sick HB101..... pBM24 pSM453 SPT5 Xho-EagI
FB825 Betsy see notebook for making null instructions TB1.1..... pBM18 spt5-del201::LEU2
FB826 Betsy for making spt5 null; see notebook TB1.1..... pBM19 spt5-del202::LEU2
FB827 Michele Original SPT5 clone from library; insert is ~3.8kb Sau3A partial at BamHI HB101..... pMS4 YCp50 SPT5
FB828 Michele SPT5 Northern probe TB1....... pMS20 pUC19 SPT5 0.8 kb SalI internal fragment
FB829 Michele from pMS4; Southern probe TB1....... pMS22 pUC19 SPT5 3.9 kb HindIII-KpnI fragment
FB830 Michele y HB101..... pMS24 pCGS42 SPT5 HindIII-EagI
FB831 Charlie complete lacZ in pSEY101; see notebook HB101..... pCHY22 URA3 lacZ 2 micron
FB832 Charlie see notebook HB101..... pCHY24 fbp1-lacZ
FB833 Gerry Johnston CDC68 clone from the YEp24 library RR1.1..... pSC2-1 CDC68 2 micron URA3 amp
FB834 Steve Hahn from Steve Hahn; do not give out; do not use .R1.1..... pSH229 GAL-SPT15
FB835 Mark Johnston see notebook HB101..... pBM311 GAL1-HIS3 fusion URA3
FB836 Mark Johnston see notebook .B101..... pBM262 GAL1-HIS3 fusion URA3
FB837 Michael Smith from Louis Lefebvre and Michael Smith; see notebook HB101..... pLL29 his4-912delta::lacZ 2 micron
FB838 Michael Smith see notebook HB101..... pLL42 HIS4-lacZ 2 micron
FB839 Michael Smith see notebook HB101..... pLL53 his4-912delta::lacZ CEN
FB840 Michael Smith see notebook HB101..... pLL54 HIS4::lacZ CEN
FB841 Mark Rose low copy plasmid (pSC101) in E. coli HB101..... pMR779 URA3 tet amp low copy
FB842 Mark Rose Low copy number plasmid in E. coli HB101..... pMR864 LEU2 CEN4 ARS1 amp low copy
FB843 DH5alpha Bob Moreland good transformation strain DH5alpha.. see notebook
FB844 M.A. Osley This plasmid is originally from L. Guarente and is also entered elsewhere in this collection HB101.ha.. pBL101 CYC-lacZ CEN URA
FB845 M.A. Osley HB101.ha.. pdeltrt2URA3 see notebook
FB846 Bob Moreland 80 amino acids HB101.ha.. pfusA1-1 HTB2-lacZ
FB847 Dave RI-Bam in RI-Bam HB101.ha.. pDE60-6 pRS304 SPT15
FB848 Dave HB101.ha.. p969-1 spt15-122 on SPT15 Cla-Sac in pRS316
FB849 Dave spt15-135 rescued by gap repair HB101..... p771-5 pRS316 spt15-135 Cla-Sac
FB850 Dave gap repair of pDE38-9 HB101..... p1259-1 spt15-122 pRS316
FB851 Joel TB1.1..... pJH6 pUC18 LEU2 Sal-Xho
FB852 Joel Opposite orientation of pJH6 (FB851) TB1.1..... pJH7 pUC18 LEU2 Sal-Xho
FB853 Joel see notebook HB101..... pJH8 pUC18 del(hta1-htb1)::LEU2
FB854 Joel see notebook FB274..... dam-3 pJH3 del(hta1-htb1)
FB855 Karen see notebook HB101..... pKA4 pSP64 SPT15 with SalI site 18 bp upstream of ATG
FB856 via Rick Wobbe "Sawadogo & Roeder, PNAS 82, 4394; Cell 43, 165. See notebook " HB101..... pML(C2AT)19del-50 amp Adeno2 major late promoter + G-less cassette
FB857 Martin Schmidt For TFIID overexpression; DO NOT GIVE OUT; see notebook N5a5a..... lambda lysogen with cI857 pASY2D SPT15 under lambda control
FB858 D. Beach .eod "Plasmid from Maureen McLeod and David Beach; if inserts are cloned into eith er BclI or SmaI, the plasmid will express tetR. " FB274..... pWH5 amp lambda cI
FB859 "Parent, Kramer PHO5 probe (?) see notebook HB101..... p20L amp PHO5 pBR322
FB860 Anne Chiang SPT9 candidate E from YCp50 library HB101..... pAC1 SPT9 URA3 amp
FB861 Anne Chiang no SPT9 function; see notebook TB1.1..... pAC10 "SPT9 subclone, Bam deletion
FB862 Anne Chiang probably not functional TB1.1..... pAC12 pRS306 SPT9 Bam-Sal
FB863 Anne Chiang partial SPT9 function TB1.1..... pAC14 SPT9 Mlu deletion clone in YCp50
FB864 Anne Chiang no SPT9 function .B1.1..... pAC16 YCp50 SPT9 HindIII deletion clone
FB865 Anne Chiang no SPT9 function .B1.1..... pAC18 YCp50 SPT9 BglII clone
FB866 Anne Chiang probably non-functional; should be rechecked TB1.1..... pAC20 pRS316 SPT9 region Bam fragment
FB867 Anne Chiang not tested for function .B1.1..... pAC22 "SPT9, SacI piece reclosed
FB868 Christine see notebook HB101..... pSPT8del15 spt8 acidic deletion 1.5 using oligo D1
FB869 Christine deletion of amino acids 39-76 using oligo D7 HB101..... pSPT8del6.20 spt8 acidic deletion 6.20
FB870 Dave 2.2 kb Sal-Xho LEU2 into Sal site in polylinker of pSE360 TB1.1..... pDE48-1 LEU2 CEN ARS URA3
FB871 Dave see notebook TB1.1..... pDE51-5 spt15::LEU2 YCp50
FB872 Dave TB1....... pDE53-1 pRS306 spt15-122
FB873 Dave TB1....... pDE54-1 pRS306 spt15-135
FB874 Dave y TB1....... pDE55-1 pCGS42 spt15-21
FB875 Dave TB1....... pDE56-1 pRS306 spt15-21
FB876 Dave "pSE358 is CEN ARS TRP plasmid; reference is Gene 70, 303-312 " TB1....... pDE58-1 spt15-21 pSE358
FB877 Dave For gap rescue of spt15 alleles; digest with RI; use largest fragment; see F B900 for URA3 version TB1....... pDE59-1 pRS314 SPT15 6.6kb Cla-Sac fragment
FB878 Dave TB1....... pDE67-3 pCGS42 spt15-122
FB879 Dave "pSE358 reference is Gene 70, 303-312 " TB1....... pDE68-4 pSE358 (CEN TRP ARS) spt15-122
FB880 Dave Both SPT15 and TRP1 are inserted in this plasmid; see notebook TB1....... pDE71-11 pRS316 SPT15 TRP1

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