Bacteria

Displaying 1 - 500 of 2807

Strain Name	Strain Alias	Source	Comments	Host	Host Markers	Plasmids	Plasmid Markers
FB1136	EB153	M. Tyers + E. Elion	GAL1-CLN3-1 (dominant mutation) fusion	HB101.....		pCGS-CLN3-1	AmpR URA3 2u GAL1::CLN3-1 fusion
FB1137	EB154	M. Tyers + E. Elion	GAL-CLN3 fusion	HB101.....		pCGS-CLN3	AmpR URA3 2u GAL1::CLN3 fusion
FB1138	EB142	E. Elion	fus3d6::LEU2= XmnI deletion XhoI deletion with LEU2 in Xho site. To integra te cut with NheI and HindIII	unknown...		pYEE98	AmpR fus3d6::LEU2
FB1139		Chris Clark	4.3kb BamHI frag containing SPT16 cloned into BamHI site of YEp24	HB101.....		pCC48	SPT16 2u URA3 ampR tetS
FB1140		A. Rowley + Curt Wittenburg		HB101.....
FB1141	pMAL-c	NEB	for making fusions to MBP (maltose binding protein) encoded by malE to ove rexpress proteins in E. coli	HB101.....		pMAL-c	AmpR
FB1142	pMAL-p	NEB	for making fusions to MBP (maltose binding protein) encoded by malE to o verexpress proteins in E. coli	HB101.....		pMAL-p	AmpR
FB1143	pMAL-cRI	NEB	for making fusions to MBP (maltose binding protein) encoded by malE To ove rexpress protein in E. coli	HB101.....		pMAL-cRI	AmpR
FB1144	pGN801	G. Natsoulis + J. Boeke	See Genetics 123:269	unknown...		pGN801-ura3-153d	ura3-153d TRP1 CEN AmpR
FB1145	pFC101.1	Cross via Elion	contains 6kb of yeast DNA that includes DAF1-1. It was in YCp50- but Fred C ross deleted CEN.	unknown...		pFC101.1	DAF1-1 (=CLN3?) URA3 ampR
FB1146	p810	Reed via Elion	YRp7 with a HindIII-SalI frag (4kb) containing the rest of CLN2 disrupted by LEU2	unknown...		p810	cln2::LEU2 ampR TRP1 ARS1
FB1147	p419	Reed via Elion	YEp13 with a 3kb EcoRI frag containing most of CLN1 with a TRP1 insertion	unknown...		p419	cln1::TRP1 (weak trp+) LEU2 2micron ampR
FB1148	pPT1	Patrick Tan	*Tagged SPT7*	TB1.......		pPT1	SPT7-HAI LEU2 CEN ARS ampR
FB1149	pPT4	Patrick Tan	Same as FB1148 but 56a.a. repeat sequence deleted by oligo directed mut.	TB1.......		pPT4	SPT7 LEU2 CEN ARS ampR
FB1150	pPT3	Patrick Tan	*UNtagged SPT7*	TB1.......		pPT3	SPT7 LEU2 CEN ARS ampR
FB1164	pMR79	Mark Rose .n	#NAME?	HB101.....		pMR79	URA3 CEN ARS ampR
FB1165	L15/pUN45	S. Buratowski	SPT15 with Lerner at N terminal in vector pUN45 (Elledge + Davis)	HB101.....		L15/pUN45	SPT15 with Lerner epitope at NT TRP1 CEN ARS ampR
FB1166	L15/pUN55	S. Buratowski	spt15 with Lerner at N terminal in vector pUN55	HB101.....		L15/pUN55	SPT15 with Lerner epitope at NT URA3 CEN ARS ampR
FB1167	pGP26	Greg Prelich	BglII spt6-140 genomic frag in BamHI site of YCp50.	HB101.....		pGP26	spt6-140 ampR URA3 CEN ARS
FB1168	pAB56	A. Bortvin	BglII spt6-140 frag cloned into BamHI site of YCp50 that has BglII-SmaI frag (CEN ARS) deleted. see also FB1167.	HB101.....		pAB56	spt6-140 URA3 ampR
FB1169	pAB1	A. Bortvin	3.5kb SPT5 frag from pMS4 cloned into SmaI site of pJK1521. LexA-SPT5 fusio n= 12-1064 a.a.	HB101.....	sick	pAB1	SPT5-lexA TRP1 CEN ARS ampR
FB1170	pAB57	A. Bortvin	same as FB1169 but fusion of lexA with SPT5 starting at a.a.#632-1064.	HB101.....	sick	pAB57	SPT5-lexA TRP1 CEN ARS ampR
FB1171	pCC74	Chris Clark	URA3 HindIII frag cloned into the trt1 deletion site in pCC72. Note: URA3 o rientation #1.	FB1.1.....	pyrf-	pCC74	(hta1-htb1)d::URA3 ampR
FB1172	MH1	Lorraine Marsh	*Good for transformation with Smash and Grab DNA* see enclosed sheet in notebook for transformation procedure.	MH1.1.....
FB1173	pCC45	Chris Clark	5.5kb Bgl frag of SPT6 cloned into BamHI site of YEp13. (orientation I) H IGH COPY SUPPRESSION	HB101.....		pCC45	SPT6 LEU2 2micron ampR
FB1174	Struhl#wt	Struhl	"TATA mut. in this plasmid= WT, but with RI and Sac sites flanking the HIS3 T ATA.REF= MCB dec89 p.5298-5304 Harbury + Struhl *SEE NOTEBOOK* "	HB101.....		pwt	URA3 ampR his3-RIS integrating plasmid
FB1175	Struhl#201	Struhl	TATA mut. in this plasmid is AATAAA. REF= MCB dec89 p. 5298-5304 Harbury + Struhl. **SEE NOTEBOOK**	HB101.....		p201	URA3 ampR his3-201 integrating plasmid
FB1176	Struhl#202	Struhl	TATA mut.= CATAAA. REF= MCB dec89 p. 5298-5304 Harbury + Struhl *SEE N OTEBOOK*	HB101.....		p202	URA3 ampR his3-202 integrating plasmid
FB1177	Struhl#203	Struhl	TATA mut.= GATAAA. REF= MCB dec89 p. 5298-5304 Harbury + Struhl *SEE N OTEBOOK*	HB101.....		p203	URA3 ampR his3-203 integrating plasmid
FB1178	Struhl#204	Struhl	TATA mut.= TCTAAA REF= MCB dec89 p.5298-5304 Harbury + Struhl *SEE NO TEBOOK*	HB101.....		p204	URA3 ampR his3-204 integrating plasmid
FB1179	Struhl#205	Struhl	mutant TATA seq= TGTAAA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p205	ampR URA3 his3-205 integrating plasmid
FB1180	Struhl#206	Struhl	mutant TATA seq= TTTAAA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p206	ampR URA3 his3-206 integrating plasmid
FB1181	Struhl#208	Struhl	mutant TATA seq= TACAAA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p208	ampR URA3 his3-208 integrating plasmid
FB1182	Struhl#209	Struhl	mutant TATA seq= TAGAAA REF: MCB dec89 p. 5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p209	ampR URA3 his3-209 integrating plasmid
FB1183	Struhl#210	Struhl	mutant TATA seq= TATCAA REF: MCB dec89 p. 5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p210	ampR URA3 his3-210 integrating plasmid
FB1184	Struhl#212	Struhl	mutant TATA seq= TATTAA REF: MCB dec89 p.5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p212	ampR URA3 his3-212 integrating plasmid
FB1185	Struhl#213	Struhl	mutant TATA seq= TATACA REF: MCB dec89 p.5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p213	ampR URA3 his3-213 integrating plasmid
FB1186	Struhl#214	Struhl	mutant TATA seq= TATAGA REF: MCB dec89 p.5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p214	ampR URA3 his3-214 integrating plasmid
FB1187	Struhl#215	Struhl	mutant TATA seq= TATATA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p215	ampR URA3 his3-215 integrating plasmid
FB1188	Struhl#217	Struhl	mutant TATA seq= TATAAG REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p217	ampR URA3 his3-217 integrating plasmid
FB1189	Struhl#218	Struhl	mutant TATA seq= TATAAT REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p218	ampR URA3 his3-218 integrating plasmid
FB1190	Struhldoub	Struhl	mutant TATA seq= TATATAC REF: MCB dec89 p.5298-5304 Harbury and Struhl * SEE NOTEBOOK!*	HB101.....		p215/238	"ampR URA3 his3-215,238 integrating plasmid
FB1191	B1295	Broach Lab		unknown...		pB1295	MATa LEU2 CEN ARS ampR
FB1192	B1311	Broach lab	MATb (alpha) Hind3 cloned from LCIII 3a into YCp50	unknown...		pB1311	MATb URA3 CEN ARS ampR
FB1193	pMS23	M. Swanson	~4kb Hind3-Kpn of pMS4 cloned into Hind3-Kpn of pRS316. SPT5 is truncated in the repeat region; partially comp. spt5-. REF: MCB 11: 3009-3019. 1991	TB1.own...		pMS23	SPT5 URA3 ampR bluescript
FB1194	pJN22	Hans Ronne	#NAME?	HB101.n...		pJN22	mig1-82::LEU2 ampR
FB1195	pJN41	Hans Ronne	#NAME?	HB101.n...		pJN41	mig1-82::URA3 ampR
FB1196	p125-3	D. Eisenmann	~6.3kb Xho-Xho piece from original SPT15 clone-- located 3' to the SPT15 gen e and cloned into pRS316	HB101.n...		p125-3	URA3 CEN ARS ampR
FB1197	pDE18-1	D. Eisenmann	~5.5kb Cla-Bam subclone with SPT15+ activity cloned into Cla-Bam of pRS316 ( see sheet inserted with FB1196)	HB101.n...		pDE18-1	SPT15 URA3 CEN ARS ampR
FB1198	pDE19-1	D. Eisenmann	3.6kb Bam-Cla frag located 3' of SPT15 gene cloned into Bam-Cla of pRS316 (s ee sheet inserted with FB1196)	HB101.n...		pDE19-1	URA3 CEN ARS ampR
FB1199	pDE23-8	D. Eisenmann	~5kb Hind3 piece containing part of SPT15 gene into pRS316 Hind3 site. do es NOT have 15+ activity because the last 13aa are not contained in this f rag. (see sheet inserted with FB1196)	HB101.n...		pDE23-8	URA3 CEN ARS ampR
FB1200	pDE24-8	D. Eisenmann	~4.4kb Hind3 frag located 3' to SPT15 gene (including CT of SPT15) cloned in to pRS316. (see sheet inserted with FB1196)	HB101.n...		pDE24-8	URA3 CEN ARS ampR
FB1201	pDE26-1	D. Eisenmann	2.4kb EcoRI-Bam piece containing SPT15 in YCp50. = original subclone of SPT 15 (see sheet inserted with FB1196)	HB101.n...		pDE26-1	SPT15 URA3 CEN ARS ampR
FB1202	pDE27-3	D. Eisenmann	Cla-Xba piece containing truncated SPT15 gene in pRS316 [derived by Xba dige stion of pDE18-1= FB1197] see sheet inserted with FB1196	HB101.n...		pDE27-3	URA3 CEN ARS ampR
FB1203	pDE69-1	D. Eisenmann	2.4kb RI-Bam piece containing spt15-21 in pRS316 (spt15-21 gene contains Sal I site upstream of the ATG put in by K. Arndt)	HB101.n...		pDE69-1	spt15-21 URA3 CEN ARS ampR
FB1204	p969-1	D. Eisenmann	original gap rescued plasmid containing spt15-122 in pRS316. pDE38-9 was us ed to rescue this allele from FW969.	HB101.n...		p969-1	spt15-122 URA3 CEN ARS ampR
FB1205	p971-5	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 from strain FW971 into pRS 316	HB101.n...		p971-5	spt15-135 URA3 CEN ARS ampR
FB1206	p1259-1	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 and strain FW1259 into pRS 316	HB101.n...		p1259-1	spt15-21 URA3 CEN ARS ampR
FB1207	pFM23-1	D. Eisenmann	"original plasmid from gapped rescue using pDE38-9 from FY183 (containing spt 15-23) into pRS316. Although this plasmid fails to complement 15-21 for h is+ phen., neither DME nor SLR could find a base change in the coding regi on. "	HB101.n...		pFM23-1	spt15-23 URA3 CEN ARS ampR
FB1208	pHCL17	D. Eisenmann	"HCS= high copy suppressor. = plasmid from YEp24 High Copy Library that suppr esses lys- phen. of spt15-21, 15-122, spt3d (strains become his+ lys+/-) see also FB 1209, 1210. "	HB101.n...		pHCL17	2 micron URA3 ampR HCS of spt15
FB1209	pHCL26	D. Eisenmann	"HCS= high copy suppressor. plasmid from YEp24 High Copy Library that suppre sses lys- phen. of spt15-21, 15-122 spt3d. (strains become his+ lys+/-) S ee also FB1208,1210. "	HB101.n...		pHCL26	2 micron URA3 ampR HCS of spt15
FB1210	pHCL30	D. Eisenmann	"HCS= High Copy Suppressor. =plasmid from YEp24 High Copy Library that suppre sses lys- phen. of spt15-21, 15-122, spt3d (strains become his+ lys+/-) See also FB1208, 1209. "	HB101.n...		pHCL30	2 micron URA3 ampR HCS of spt15
FB1211	pSR7-1	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 from spt15-21 intragenic r evertant strain SR7 (into pRS316). AA change = F237V and G174E see also FB1212.	HB101.n...		pSR7-1	spt15-21R7 URA3 CEN ARS ampR
FB1212	pSR31-1	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 from spt15-21 intragenic r evertant strain SR31 (into pRS316). AA change=F237V and G174E same as fo r FB1211's SR7.	HB101.n...		pSR31-1	spt15-21R31 URA3 CEN ARS ampR
FB1213	pSR47-1	D. Eisenmann	"original plasmid from gapped rescue using pDE38-9 from spt15-21 intragenic r evertant strain SR47 (into pRS316). AA change= F237V and G174E See also FB1211, 1212. "	HB101.n...		pSR47-1	spt15-21R47 URA3 CEN ARS ampR
FB1214	pDE79-3	D. Eisenmann	spt15-237 (aa change= F237V; intragenic rev) in wild type background. (done by 3way ligation) It complements 15-21 and has no spt- phen. whem shuffled over the null.	HB101.n...		pDE79-3	spt15-237 TRP1 CEN ARS ampR
FB1215	pDE103-1	D. Eisenmann	~2.5kb RI-BglII piece from pFW27 containing SPT3 into pUC118 RI-Bam.	HB101.n...		pDE103-1	SPT3 ampR
FB1216	pDE121-6	D. Eisenmann	SPT3 from plasmid pDE105-6 using primers ODE36 and ODE38. These put BamHI s ites at NT and CT of SPT3 open reading frame. Bam cut product was subclone d into pRS316 Bam.	HB101.n...		pDE121-6	SPT3 URA3 CEN ARS ampR
FB1217	pDE121-8	D. Eisenmann	see FB1216 for comments (pDE121-8 is a diff. isolate).	HB101.n...		pDE121-8	SPT3 CEN ARS URA3 ampR
FB1218	p3-401	D. Eisenmann	original plasmid from gapped rescue using pDE105-6 cut with MluI and XhoI in to spt15-21 revertant strain L805. p3-401 is in pMR79(=YCp50 with XhoI sit e knocked out)	HB101.n...		p3-401	spt3-401 URA3 CEN ARS ampR
FB1219	p3-445	D. Eisenmann	spt3-445 rescued from FY652 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-445	spt3-445 URA3 CEN ARS ampR
FB1220	p3-1	D. Eisenmann	spt3-1 rescued from FW240 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-1	spt3-1 URA3 CEN ARS ampR
FB1221	p3-426	D. Eisenmann	spt3-426 rescued from FY648 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-426	spt3-426 URA3 CEN ARS ampR
FB1222	p3-429	D. Eisenmann	spt3-429 rescued from FY649 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-429	spt3-429 URA3 CEN ARS ampR
FB1223	p3-417	D. Eisenmann	spt3-417 rescued from FY647 into pMR79 (=YCp50 with Xho site knocked out).	HB101.....		p3-417	spt3-417 URA3 CEN ARS ampR
FB1224	p3-441	D. Eisenmann	spt3-441 rescued from FY650 into pMR79 (=YCp50 with Xho site knocked out).	HB101.....		p3-441	spt3-441 URA3 CEN ARS ampR
FB1225	p3-443	D. Eisenmann	spt3-443 rescued from FY651 into pMR79 (=YCp50 with Xho site knocked out).	HB101.....		p3-443	spt3-443 URA3 CEN ARS ampR
FB1226	pDE124-1	D. Eisenmann	~2.3kb RI-Xho frag from FB1218 (p3-401) into RI-Xho of pRS306 (for integrat ion of spt3-401).	HB101.....		pDE124-1	spt3-401 URA3 ampR
FB1227	pDE126-1	D. Eisenmann	~2.3kb RI-Xho frag containing spt3-429 from FB1222 (p3-429) into pRS306 RI-X ho (for integration of spt3-429)	HB101.....		pDE126-1	spt3-429 URA3 ampR
FB1228	pDE128-1	D. Eisenmann	~2.3kb RI-Xho frag containing spt3-445 from FB1219 (p3-445) into RI-Xho of p RS306. (for integration of spt3-445)	HB101.....		pDE128-1	spt3-445 URA3 ampR
FB1229	pDE132-23	D. Eisenmann	SPT3 with Bam ends derived by PCR (see FB1216) cloned into pMA424 to create an in frame fusion of GAL4 1-147 to all of SPT3. (has SPT3+ activity)	HB101.....		pDE132-23	GAL4-SPT3 fusion HIS3 2micron ampR
FB1230	pMG1	M. Gandhi .	SalI-SpeI frag of SPT8 cloned into pRS316.	TB1.1.....		pMG1	SPT8 URA3 CEN ARS ampR
FB1231	pGAD10	S. Fields	see enclosed sheet.	HB101.....		pGAD10	GAL4 LEU2 ampR
FB1232	pKA67	Ricupero/Arndt	RI-Bam frag containing spt15-122 from pKA21 subcloned into RI-Bam of pRS316. (SalI site is present at spt15-122 ATG)	HB101.....		pKA67	spt15-122 URA3 CEN ARS ampR
FB1233	pKA68	Ricupero/Arndt	~2.4kb RI-Bam frag containing spt15-301 from pKA14 subcloned into RI-Bam of pRS316. (SalI site is present at spt15-301 ATG)	HB101.....		pKA68	spt15-301 URA3 CEN ARS ampR
FB1234		Nasmyth	2micron LEU2 library in YEp13 (2 vials frozen)	unknown...		2micron LEU2 library	2micron LEU2 library
FB1235	pKA71	S. Ricupero	HA mutagenized spt15-3 (FB998) to get intragenic suppressors of spt15-122 (s elected Lys+). This isolate= #13a A.A. change= P191S (CCT to TCT) + L205F (15-122) see also FB1236-1238.	HB101.n...		pKA71	spt15-319 ampR CEN ARS TRP1
FB1236	pKA72	S. Ricupero	pKA71= intragenic sup candidate #13b. SEE FB1235	HB101.n...		pKA72	spt15-319 ampR CEN ARS TRP1
FB1237	pKA73	S. Ricupero	PKA73= intragenic sup candidate #19. SEE FB1235	HB101.n...		pKA73	spt15-319 ampR CEN ARS TRP1
FB1238	pKA74	S. Ricupero	pKA74= intragenic sup candidate# 33. SEE FB1235	HB101.n...		pKA74	spt15-319 ampR CEN ARS TRP1
FB1239	Struhl#216	Ricupero .	mutant TATA seq= TATAAC. REF= MCB dec89 p.5298-5304 Harbury and Struhl **S EE ALSO FB1174-1190)	HB101.n...		p216	URA3 ampR integrating plasmid his3-216
FB1240	pCC10	Christy	~6kb XhoI-Hind3 frag from pCC27 into SalI-Hind3 sites of YCp50.	HB101.n...		pCC10	spt6 subclone ampR URA3 CEN ARS
FB1241	pCC19	Christy	Pvu-Sal 3.8kb frag containing SPT6 from pCC11 subcloned into YCp50 Pvu-Sal.	HB101.n...		pCC19	spt6 subclone URA3 CEN ARS ampR
FB1242	pCC32	Christy	Hind3-Hind3 spt6 subclone (~1.5kb) cloned into YCp50	HB101.n...		pCC32	spt6 subclone URA3 CEN ARS ampR
FB1243	pGEX-1	Glutagene (Cepko lab)	see sheet inserted in notebook.	HB101.n...		pGEX-1	for fusions with glutathione-S-tranferase
FB1244	pGEX-2T	Glutagene (Cepko lab)	see sheet inserted in notebook.	HB101.n...		pGEX-2T	for glutathione-S-transferase fusions
FB1245	pGEX-3X	Glutagene (Cepko lab)	see sheet inserted in notebook.	HB101.n...		pGEX-3X	for glutathione-S-tranferase fusions
FB1246	pPYKc/g	R. Kornberg	see sheet inserted in notebook.	HB101.n...		pPYKc/g	PYKI promoter ampR
FB1247	pCC60	Chris Clark	5.7kb SalI frag from HTA1-HTB1 cloned into the SalI site of YCp50	HB101.....		pCC60	HTA1-HTB1 URA3 tetS ampR
FB1248	pCC72	Chris Clark	1.4kb HTA1-HTB1 Hind3 frag was cloned into hind3 sites of pUC13-d16b (FB584= arsd of HTA1-HTB1) which has lost the 1.1kb H3 promoter frag.	HB101.....		pCC72	HTA1-HTB1d ampR
FB1249	pJH20	Hirschhorn	1.8kb StyI frag of Yip5 TRT2 into StyI site of pBR322	HB101.....		pJH20	HTA2-HTB2 truncated ampR TcR
FB1250	pJH21	Hirschhorn	TRP1+ Bam-Bgl frag from pBM453 (FB451) into BglII of pJH20 (FB1249)	HB101.....		pJH21	(hta2-htb2)d::TRP1 ampR TcR
FB1251	pJH23	Hirschhorn	Bam-SacII 2.6kb frag from pCC60 (FB1247) into Bam- SacII of pRS313	unknown...		pJH23	HTA1-HTB1 HIS3 CEN ampR
FB1252	pJH24	Hirschhorn	RI-Bam HTA2-HTB2 frag from Yip5 into RI-Bam of pRS316	unknown...		pJH24	HTA2-HTB2 URA3 CEN ampR
FB1253	pJH28	Hirschhorn	1.1kb Hind3 frag from pRB58 (FB997) into Hind3 of pRS316	TB1.own...		pJH28	SUC2 promoter URA3 CEN ampR
FB1254	pJH29	Hirschhorn	~160bp RI-Hind3 frag from pJH28 (FB1253) --upstream of SUC2-- into RI-Hind3 sites of pUC19	TB1.own...		pJH29	SUC2 upstream ampR
FB1255	pJH26	Hirschhorn	Bam-SalI frag from pJH24 (FB1252) into Bam-SalI of pRS424	TB1.......		pJH26	HTA2-HTB2 TRP1 2micron ampR
FB1256	pJH31	Hirschhorn	Bam-SalI frag fron pJH24 (FB1252) into Bam-SalI of pRS425	TB1.......		pJH31	HTA2-HTB2 LEU2 2micron ampR
FB1257	pJH35	Hirschhorn	H3-H3 frag from pJH28 (FB1253) into H3 of pUC19	TB1.......		pJH35	SUC2 promoter ampR
FB1258	pJH38	Hirschhorn	pJH35 (FB1257) MluI NcoI filled in and YEp24 H3 (URA3 frag) filled in	FB600.....	pyrf	pJH38	suc2-101::URA3 ampR
FB1259	pJH42	Hirschhorn	1. RI-H3 from pJH28 (FB1253) into RI-H3 of mp19. 2. dut ung mut. with S2Tk (TATAAA to GGTACC). 3. RI-H3 frag into RI-H3 of pUC19.	TB1.0.....		pJH42	suc2-104 ampR
FB1260	RZ1032	B. Berg	for dut ung mutagenesis	RZ1032....	dut1 ung1 thi1 relA HfrKL16 PO/45 [lysA61-62] 2bd-279::Th10 supE44
FB1261	pCT1	Rick Young	*DO NOT GIVE OUT!!*	DH5alpha..		pCT1	srb2-1 URA3 CEN ARS ampR
FB1262	pCT19	Rick Young	*DO NOT GIVE OUT!!*	DH5alpha..		pCT19	SRB2 URA3 CEN ARS ampR
FB1263	BC9b	Brendan Cormack (Struhl)	*There is ANOTHER SmaI site in the pBR portion*	HB101.ha..		pBC9b	DED1 promoter GCN4 3'UT URA3 CEN ampR
FB1264	GN1561-1	Georges Natsoulis	MNase engineered so that coding region is flanked by BamHI and SalI sites-- then cloned into pUC19. *ONLY mature protein coding seq.--NO ATG!!* REF: Nature 352:632-5 for 5' Bam site.	HB101.ha..		pGN1561-1	Micrococcal Nuclease (staphylococcal Nuc.) ampR
FB1265	pCC27	Chris Clark	SPT6 in YCp50.	unknown...		pCC27	SPT6 URA3 CEN ARS ampR
FB1266	GAL1-cDNA	Tony Bretscher	GAL1-cDNA library REF: Genetics 132 (1992) p. 665-673	DH10B			GAL1-cDNA library
FB1267	771	Roger Brent	EcoRV-RsaI frag containing cyc1-512 into StuI site of pRB486 (see enclosed s heet)	HB101.n...		p771	cyc1-512 terminator ampR
FB1268	798	Roger Brent	EcoRV-RsaI containing CYC1 into StuI site of pRB486 (see enclosed sheet).	HB101.n...		p798	CYC1 terminator ampR
FB1269	pPK606	M. Grunstein	REF: CELL 55:27-39	MH1.......		pPK606	hhf2d(4-23) CEN URA3 ampR
FB1270	pPK617	M. Grunstein	REF: CELL 55:27-39	MH1.......		pPK617	hhf2d(4-14) CEN URA3 ampR
FB71		Carl Falco	Yip5+lys2 R1-H3 fragment	HB101.....		SL42-2	ura3 ampR lys2
FB72		Carl Falco	Yip5+lys2 R1-Cla1 fragment	HB101.....		SL46-1	ura3 ampR lys2
FB73	6507/pFW40	FRED	"same as pFW38 but 2 sal fragments are in the same orientation. Yip5, R1-H3 ars1, H3-BamH1 CEN3, HIS4 sal fragment "	unknown...		pFW40	ars cen ampR
FB1	DB6507	Botstein	Can screen for URA3 function	..........	pyrF74::Tn5 and all HB101 markers	none
FB2	B44/YIp5	Fink		DB6507....	HB101 pyrF-74::Tn5	YIp5	amp tet URA3
FB3	B44/YIp5	G.R. Fink	"plasmid very unstable in yeast, even when selected "	DB6507....	HB101 pyrF74::Tn5	YRp10	"ARS1, URA3, amp
FB4	B28/TR5695	J.R. Roth via G.R. Fink		TR5695....	trp B1 his (P2 eductant)	none
FB5	TR5673	J.R. Roth	grows in histidinol	TR5695....	trp B1 his (P2 eductant)	F' hisG+D+	hisG+D+
FB6	RB18	Botstein		DB6656....	trp(am) lac(am) pyrF::Mu su-	YIp32	"LEU2, amp
FB7	RB19	Botstein	"Gene 8, 17-24 (1979) "	DB6656....	trp(am) lac(am) pyrF::Mu su-	YIp33	"LEU2, amp
FB8	RB20	Botstein	"Gene 8, 17-24 (1979) "	DB6656....	trp(am) lac(am) pyrF::Mu su-	YIp29	LEU2 URA3 amp
FB9	RB21	Botstein	"Gene 8, 17-24 (1979) "	DB6656....	trp(am) lac(am) su- pyrF::Mu	YIp28	LEU2 URA3 amp
FB10	SF1032	Froshauer	MC4100 genetic background	SF1032....	srl::Tn10 reca ara relA lac(del) strR
FB11	none	Winston	insert is 5.3 kb	B28.32....	trp B1 his (P2 eductant)	pFW1	"E. coli hisOGD H3 in YIp5, amp
FB12	none	Winston	opposite orientation of pFW1	B28.32....	trp B1 his (P2 eductant)	pFW2	"E. coli hisOGD in YIp5, amp, weak tet
FB13	B44/pFW3	Winston	YIp5 with the HindIII site destroyed by filling in with reverse transcriptas e	DB6507....	HB101 pyrF74::Tn5	pFW3	URA3 amp
FB14	B44/pFW4	Winston	SalI site destroyed by filling in with reverse transcriptase	DB6507....	HB101 pyrF74::Tn5	pFW4	"URA3, amp
FB15	B44/pFW5	Winston	orientation 1	DB6507....	HB101 pyrF74::Tn5	pFW5	"URA3, amp, HIS4 EcoRI fragment
FB16	B44/pFW6	Winston	orientation 2	DB6507....	HB101 pyrF74::Tn5	pFW6	"URA3, amp, HIS4 EcoRI fragment
FB17	M182pMC593	Casadaban	9.6 kb	M183.7....	del(lacIPOZY)X74 galK galU strA	pMC593	"kan, amp lac
FB18	MC1061	Casadaban	There are two entries of this strain in the FB collection	MC1061....	"araD139 del(ara,leu)7697 dellacX74 galE galK hsr- hsm+ strA	none
FB19	M8820F1	Casadaban		M8820F1...	"araD139 del(ara,leu)7697 del(proABlacIPOZYA)X111 strA ph80 ph80dF1arat rplac	none
FB20	DB6660	Botstein		DB6660....	trp(am) lac(am) pyrF::Mu hsr- hsm+ supF	none
DB21	RB39	Botstein	"ura3-18 on Bam fragment, LEU2 on Sal/Xho "	DB6660....	see FB20	pRB39	amp ura3-18 LEU2
FB22	B28lam322	Ralph Isberg		B28.60....	see FB4	lambda 322	b5a5 cI857 nin Sam7 Tn903(b2)
FB23	E. coli	J. Davies	pAJ1 is a dimer of YEp13 and pRZ102 which is a mini colE1 with Tn5	E. coli...		pAJ1	LEU2 Tn5(kan) 2 micron
FB24	none	J. Davies	pAJ50 is YEp13 and pA043 which is mini colE1 with Tn903)	E. coli...		pAJ50	kan LEU2
FB25	B44/pFW9	Winston	The his fragment is RI-Pst. The plasmid is ampS tetR.	DB6507....		pFW9	URA tet Salmonella hisOGD
FB26		Dickson	Check Fred's notes; this is the b-gal gene from K. lactic	DB6507....		pKl6	K. lactis b-gal
FB27		Dickson	"see Fred's notes, contains K. lactis b-gal "	DB6660....		pKl6	K. lactis b-gal
FB28		Winston		DB6507....		pFW10	"tet, URA3, Salmonella hisOGD, ars1
FB29		Johnson and Abelson	"SUP6 is an Alu2 fragment with Bam linkers, 750 bp long, tRNAtyr "	DB6507....		pYSUP6-Alu2	"amp, SUP6
FB30		Winston	HIS4 Sal fragment in YIp5; lacZ Xho fragment in Xho site in HIS4; lacZ fragm ent is from pMC593	DB6507....		pFW11	amp URA3 lacZ HIS4
FB31		Winston	"same as pFW11, but opposite lacZ orientation "	DB6507....		pFW12	"amp, HIS4, URA3, lacZ
FB32	PNN49			LE392.....	met r- m+ SupE SupF	YRp14
FB33	PNN50			LE392.....	met r- m+ SupE SupF	YRp15
FB34		Winston	SUP6 Bam fragment in YEp13	DB6507....		pFW13	LEU2 SUP6 2micron
FB35		Winston	"derived from YIp5 and pYeCEN3-41; see Nature 287, 504 "	DB6507....		pFW14	ars1 CEN3 URA3 amp
FB36		Winston	YIp5 with the HIS4 5' RI fragment; URA3 is inserted in the HIS4 HindIII site	DB6507....		pFW7	URA3 HIS4
FB37		Winston	same as pFW7 except opposite URA3 orientation; in this plasmid it is in the sameorientation as HIS4	DB6507....		pFW8	URA3 HIS4 amp
FB38			pAJ50 entered elsewhere	JM101.....	(lac pro)del supE B1/ F' traD36 proAB LacIq lac7M15	pAJ50
FB39		Winston	used for 3' experiment	DB6507....		pFW15	amp HIS4 241 bp H3-RI fragment
FB40		Winston	used for 3' experiment	DB6507....		pFW16	amp HIS4 279 bp H3-RI
FB41		Winston	Original SPT3 clone candidate 1.6	DB6507....		pFW17	URA3 2 micron SPT3
FB42		Winston	Original SPT3 clone 2.37(3-4)	DB6507....		pFW18	URA3 SPT3 2 micron amp
FB43		Winston	Original SPT3 clone 3.1	DB6507....		pFW19	URA3 SPT3 2 micron amp
FB44		Winston		DB6507....		pFW21	YRp10 with SPT3 1.7 Sal fragment
FB45		Winston .		DB6507....		pFW20	YRp10 with 1.2 kb RI fragment from SPT3
FB46		Winston	This clone has SPT3 function	DB6507....		pFW23	pCGS142 with SPT3 H3-BglII fragment
FB47		Winston		DB6507....		pFW24	pBR322 with SPT3 H3-BglII fragment
FB48		Winston	pFW27= pFW4 with 8kb HindIII frag containing SPT3	DB6507....		pFW27	SPT3 URA3 ampR
FB49		Winston		DB6507....		pFW22	pFW19 with 2 small Sal fragments deleted
FB50		Winston		DB6507....		pFW28	pFW27 with 1.7 kb Sal fragment deleted
FB51		G. Rubin and A. Spradling		E. coli...		pPi25.1	pBR322 with SalI fragment from Drosophila containing a P element
FB52		Winston		DB6507....		pFW29	SPT3 H3-BglII URA3 amp
FB53		Winston	SPT3 fragment in pFW4	DB6507....		pFW30	SPT3 H3-BglII URA3 amp
FB54		Winston	SPT3 H3-BglII in pCGS42	DB6507....		pFW31	SPT3 H3-Xho URA3 amp 2 micron
FB55		Winston		DB6507....		pFW32	SPT3 RI-BglII URA3 2 micron amp
FB56		Winston	kanR from Tn903 on PvuII fragment in pBR PvuII site	HB101.....		pFW35	amp kan tet
FB57		Winston	"same as FB56, but isolate #4, orientations unknown "	HB101.....		pFW35	amp kan tet
FB58		Winston	Contains P element with kanR in it; candidate #10	HB101.....	pFW36	pFW36	kan amp
FB59		Winston	isolate #12	HB101.....		pFW36	same as FB58
FB60		Casadaban via Mike Hall	This plasmid in HB101 in strain FB233	M182......	dellacX74 galK galU strA	pMC1871	tet lacZ with polylinker
FB61		R. Zitomer.		E. coli...		YRpBCRC1	galK; protein fusions of CYC1 to galK
FB62		R. Zitomer		E. coli...		YRpBCR6	"galK, uses galK ATG
FB63		Winston	derived from pFW30	DB6507....		pFW33	amp URA spt3-101
FB64		Winston	spt3 deletion that goes outside the gene	DB6507....		pFW34	amp URA spt3-201
FB65		Winston	RI-Xho of SPT3 in RI-Sal of pBR322	DB6507....		pFW42	amp SPT3
FB67		Winston	"WRONG, DO NOT USE "	DB6507....		pFW44	"WRONG, DO NOT USE
FB66		Winston	RI-Xho of SPT3 in YIp5	DB6507....		pFW43	amp URA SPT3
FB68	JT503	J. Thorner	RI-H3 of MATa	DB6507....		JT503	MATa
FB69		Winston	pFW14 plus HIS4 5' Sal fragment in undetermined orientation	DB6507....		pFW38	HIS4 5' amp
FB70		Winston	same as pFW38 except has the 912delta Sal fragment	DB6507....		pFW39	his4-912delta URA amp
FB74		Winston	see FB70	DB6507....		pFW41	same as pFW39 but 2 Sal fragments inserted
FB75	MC1066pAM8	Casadaban		MC1066....		pAM8	ars1 trp1 leu2-lacz
FB76	MC1066	Casadaban	Integrating plasmid for leu2-lacz fusions	MC1066....	del(lacIPOZYA)74 leuB6 trpC9830 purF::Tn5 strA	pMC1840	amp leu2-lacZ URA3
FB77		B. Errede		HB101.....		pAB25	CYC7
FB78		Rose and Winston		DB6507....		pMRFW2	ura3-52 amp URA3
FB79		Jensen and Herskowitz		DB6507....		YCp50-HO	HO URA3 CEN4 ARS1
FB80		J. Abraham via Bik Tye	H3 fragment of MATalpho into the H3 site of YEp13	DB6507....		alpha*7.375	MATalpha LEU2 2 micron
FB81	LG90	Guarante	"Guarante and Ptashne PNAS 78,2199-2203 "	LG90.7....	F- dellacproXIII
FB82		Guarante	"Gray et al PNAS 79, 6598; for making lacZ fusions in E. coli "	LG90.7....	see FB81	pMR100	lacZ amp
FB83		Winston	HIS4 Bgl-Sal (1.2 kb) into pBR322 Bam-Sal	DB6507....		pFW45	amp HIS4
FB84		Collaborative	0.85 kb GAL fragment; for PROTEIN fusions	DB6507....		pCGS286	GAL UAS 'lacZ URA3 2 micron
FB85		Bik Tye	"MboI paritals into Bam site of pCh1. Blue in haploid, white in diploid. "	HB101(?)..		pCh1-41	"amp, LEU2, lacZ, Ty
FB86		Bik Tye	see FB85	HB101?....		pCh1-51	see FB85
FB87		Bik Tye	see FB85	HB101?....		pCh1-103	see FB85
FB88		G.S. Roeder		DB6507....		R338	amp Ty1-17 URA3
FB89		Winston	deletion of Xho fragment in R338	DB6507....		pFW46	amp URA3 1-17delta
FB90	B6	via Fink		?.6507....		pBR322
FB91	B46	via Fink		?.6507....		YEp13	LEU2 amp 2 micron
FB92	B161	Fink	"Ty probe, internal "	?.6507....		B161	"amp, Ty 1.2 BglII fragment
FB93	B205	Fink	Ty2 probe	?.6507....		Ty917 Cla	amp cam Ty917 Cla fragment
FB94	B255	Fink		..6507....		YEp24	amp tet (5 u/ml) URA3 2 micron
FB95	B291	Collaborative	2 micron DNA contains an XBA site	?.6507....		pCGS42	URA3 tet amp 2 micron
FB96	B292	Collaborative	same as pCGS42 except 2 micron fragment in opposite orientation	?.........		pCGS44	URA3 2 micron amp tet
FB97	B331	Fink	"correct reference is Struhl et al, PNAS "	HB101.....		YIp5	URA3 tet amp
FB98	B415	Jef Boeke	G418 from Tn903 on PvuII cloned in pBR322. Also pombe ars and URA3 HindIII.	HB101.....		pJEF113	URA3 G418R (kanR)
FB99	B489	Ron Davis lab	DNA sequence available	DH5alpha		YCp50	URA3 CEN4 ARS1 amp ura
FB100	B506	Jef Boeke	912 evictant with G418R	HB101.....		pJEF177	G418R (kanR) URA3 amp
FB101	B524	Weinstock et al	"see Weinstock et al, PNAS 80, 4432, 4436 "	MH1000....	ompB101	pORF1
FB102	B525	Weinstock	see above	MH1000....	ompB101	pORF2	"see Weinstock et al PNAS 80, 4432-4436
FB103	B570	Giora Simchen	RI partial evictant oflys2-173 with pSL42-2	HB101?....		pGS49	amp URA3 lys2-173
FB104	B577	Giora Simchen	eviction of lys2-128delta R3	HB101?....		pGS64	amp URA3 lys2-128deltaR3
FB105	B620	Collaborative	GAL UAS on 0.75 kb fragment; does NOT contain the GAL +1 of transcription	.B101?....		CGE258	amp URA3 2 micron GAL UAS
FB106	B621	Collaborative	"GAL UAS on 0.75 kb fragment, Xho cloning site "	?.........			GAL UAS URA3 amp
FB107	B622	Collaborative	GAL UAS with Sal cloning site	?.........			"GAL UAS, URA amp 2 micron
FB108	B625	D. Schild via Fink	Current Genetics 7: 85-92	JA300.....	thr leuB6 thi thyA trpC117 shmK- hsrK- strR	pSM21	rad52::TRP1 amp
FB109	B626	D. Schild via Fink	see FB108	JA300.....	see 108	?	rad52::LEU2
FB110	B636	D. Schild via Fink	see Current Genetics 7:85-92.	HB101.....		YEp13-RAD52	RAD52 LEU2 2 micron amp
FB111	B638	D. Schild via Fink	see Current Genetics 7:85-92	JA300.....	see FB108	pSM13
FB112	HB101	via Fink	B324 in GR Fink collection	.A300.....	thr leu pro B1 supE44 r- m- recA
FB113	B522	Silhavy	"PNAS 80,4432 "	MH3000....	"araD139 del(ara,leu)7697 del(lac)X74 galU galK rpsL (strR) ompR101
FB114	B523	Silhavy		TK1046....	araD139 del(argF-lac)U169 rpsL150(strR) relA1 flbB5301 deoC1 ptsF25 ma lPQ::Tn5 ompBcs1
FB115	?	D. Hanahan	high frequency transformer	?.1046....	?	none
FB116	K38			K38.46....	HfrC
FB117	JM101	via Fink	This is strain B234 in the G.R. Fink collection	JM101.....	del(lac-pro) supE thi/ F'traD36 proAB lacIq	none
FB118	B155	"Fink, Phil Carter	orientation of insert unknown	?.101.....		B155	Ty912 HIS4 URA3 amp
FB119		Winston	Bgl2-Xho internal LYS2 fragment in pBR322; LYS2 probe	HB101.....		pFW47	LYS2 amp
FB120	ES942	via Colin Manoil		ES942.....	mutD5 metE lacZ trpA F-	none
FB121	LE30	via Colin Manoil	mutD mutagenesis increased by TdR (usually enough in LB)	LE30......	mutD5 rpsL (strR) azi galU95 F-	none
FB122		G. Walker	pKM101 derivative from Graham Walker	JC2926....	recA13 plus many others; look in strain notebook	pGW1514	"kanR, pKM101 derivative
FB123		Winston	partial evictant of lys2-173R2	HB101.....		pFW48	"amp, part of lys2-173R2
FB124			FB124-FB152 all derivatives of pFW32	HB101.....		pFW32-302	spt3-302
FB125				HB101.....		pFW32-306	spt3-306
FB126				HB101.....		pFW32-307	spt3-307
FB127				HB101.....		pFW32-309	spt3-309
FB128		.		DB6507....		pFW32-318	spt3-318
FB129		.		DB6507....		pFW32-319	spt3-319
FB130		.		DB6507....		pFW32-322	spt3-322
FB131		Winston		DB6507....		pFW32-326	spt3-326
FB132		Winston		DB6507....		pFW32-327	spt3-327
FB133		Winston		DB6507....		pFW32-328	spt3-328
FB134		Winston		DB6507		pFW32-330	spt3-330
FB135		Winston		DB6507....		pFW32-332	spt3-332
FB136		Winston		DB6507....		pFW32-334	spt3-334
FB137		Winston		DB6507....		pFW32-335	spt3-335
FB138		Winston		DB6507....		pFW32-336	spt3-336
FB139		Winston		DB6507....		pFW32-339	spt3-339
FB140		Winston		DB6507....		pFW32-343	spt3-343
FB141		Winston		DB6507....		pFW32-343	spt3-343
FB142		DB6507		.B6507....		pFW32-349	spt3-349
FB143		Winston		DB6507....		pFW32-354	spt3-354
FB144		Winston		DB6507....		pFW32-355	spt3-355
FB145		Winston		DB6507....		pFW32-360	spt3-360
FB146		Winston		DB6507....		pFW32-364	spt3-364
FB147		Winston		DB6507....		pFW32-367	spt3-367
FB148		Winston		DB6507....		pFW32-370	spt3-370
FB149		Winston		DB6507....		pFW32-374	spt3-374
FB150		Winston		DB6507....		pFW32-377	spt3-377
FB151		Winston		DB6507....		pFW32-379	spt3-379
FB152		Winston		DB6507....		pFW32-382	spt3-382
FB153		Christy		DB6660....	trp(am) lac(am) pyrF::Mu r- m+ supF	pCC1	SPT3 on RI-XhoI fragment in YCp50
FB154		Winston	lys2-173R2 cloned out using pSL42-2 and RI partial	HB101.....		pFW49	lys2-173R2 URA3
FB155		Winston	same as FB154 except a second isolate (b6)	HB101.....		pFW49	lys2-173R2
FB156		Winston	Bam-Sal fragment from lFB128 into Bam-Sal of pBR322. Fragment is internal t o SPT3 and is approx. 0.55 kb long.	HB101.....		pFW50	internal fragment of SPT3 in pBR322
FB157	JM103		"Find markers in most catalogs, eg NE Biolabs, BRL; M13 host "	JM103.....		F'pro
FB158	CY37	Kurjan & Herskowitz	Sau3A partial into YEp13; contains MFalpha1 (alpha factor)	MH1.3.....	araD139 lac-delX74 galU galK hsr hsm+ strA	p69A	YEp13 MFalpha1 (alpha factor)
FB159	CY17	Kurjan & Herskowitz	contains MFalpha1 (alpha factor)	RR1.3.....	F- pro leuB thi lacY hsdR hsdM ara14 gal2 xyl5 mtl1 supE44 endoI	pHK2	pBR328 with 1.6 kb RI fragment of p69A in the RI site
FB160	B90	Fink	5' Sal fragment of HIS4 in YIp5	?.1.3.....		YIp301	URA3 HIS4A
FB161	B131	Fink	RI eviction of his4-917; see map in strain book	?.1.3.....		phis4-917	URA3 his4-917
FB162		Christy	mutD generated	HB101.....		pFW32-218	spt3-218
FB163		Christy	mutD generated	HB101.....		pFW32-219	spt3-219
FB164		Christy	mutD generated	HB101.....		pFW32-220	spt3-220
FB165		Christy	mutD generated	HB101.....		pFW32-221	spt3-221
FB166		Christy	mutD generated	HB101.....		pFW32-224	spt3-224
FB167		Christy	mutD generated	HB101.....		pFW32-226	spt3-226
FB168		Christy	mutD generated	HB101.....		pFW32-228	spt3-228
FB169	B231	Fink	HIS4 region from RI site 5' to H3 site 3'. All of HIS4 is not included.	DB6507....		phis4-917delta	his4-917delta URA3 amp
FB170		Winston	deleted Cla fragment from plasmid in strain FB161.	HB101.....		pFW51	Ty917Cla deletion URA3 amp
FB171	KK2186	M. Berman	single stranded vector	KK2186....		pZ150
FB172		M. Berman	single stranded vector	KK2186....		pZ152
FB173		M. Berman		KK2186....		none
FB174	c12	Jensen and Herskowitz	may not be regulated properly	?.2186....		YCp50-HO::lacZ	HO::lacZ URA3 amp
FB175		Winston	made by eviction using YIp6 (alias YIp301?)	HB101.....		pFW52	Ty917(480) URA3 amp
FB176		Fields and Herskowitz	This contains an alpha factor-lacZ fusion in YCp50	?.101.....		YCp50::fz46	alpha factor lacZ URA
FB177		Christy		HB101.....		pFW32-232	spt3-232
FB178		Christy		HB101.....			spt3-233
FB179		Christy		HB101.....			spt3-240
FB180		Christy		.B101.....			spt3-242
FB181		Chirsty		HB101.....			spt3-242
FB182		Christy		HB101.....			spt3-243
FB183		Christy		HB101.....			spt3-244
FB184		Christy		HB101.....			spt3-245
FB185		Christy		HB101.....			spt3-246
FB186			deleted	.B101.....
FB187		Christy		HB101.....			spt3-248
FB188		Christy		HB101.....			spt3-249
FB189		Christy		.B101.....			spt3-250
FB190		Christy		HB101.....			spt3-251
FB191		Christy		HB101.....			spt3-252
FB192		Christy		HB101.....			spt3-253
FB193		Christy		.B101.....			spt3-254
FB194		Christy		HB101.....			spt3-255
FB195		Christy		HB101.....			spt3-256
FB196		Christy		HB101.....			spt3-257
FB197		Christy .		HB101.....			spt3-258
FB198		Christy		HB101.....			spt3-259
FB199		Christy		HB101.....			spt3-260
FB200		Christy		HB101.....			spt3-261
FB201		Christy		HB101.....		pFW32-262	spt3-262
FB202		Christy		HB101.....		pFW32-263	spt3-263
FB203		Christy		HB101.....		pFW32-264	spt3-264
FB204		Christy		.B101.....		pFW32-265	spt3-265
FB205		Christy		HB101.....		pFW32-266	spt3-266
FB206		Christy		.B101.....		pFW32-267	spt3-267
FB207		Christy		HB101.....		pFW32-268	spt3-268
FB208		Christy		HB101.....		pFW32-269	spt3-269
FB209		Christy		HB101.....		pFW32-270	spt3-270
FB210		Christy		.B101.....		pFW32-271	spt3-271
FB211		Christy		HB101.....		pFW32-272	spt3-272
FB212		Christy		HB101.....		pFW32-273	spt3-273
FB213		Christy		HB101.....		pFW32-274	spt3-274
FB214		Christy		.B101.....		pFW32-275	spt3-275
FB215		Christy		HB101.....		pFW32-276	spt3-276
FB216		Christy		HB101.....		pFW32-277	spt3-277
FB217		Christy		HB101.....		pFW32-278	spt3-278
FB218		Christy		HB101.....		pFW32-279	spt3-279
FB219		Christy		HB101.....		pFW32-280	spt3-280
FB220		Christy		HB101.....		pFW32-281	spt3-281
FB221		Christy		HB101.....		pFW32-282	spt3-282
FB222		Christy		HB101.....		pFW32-283	spt3-283
FB223		Christy		HB101.....		pFW32-284	spt3-284
FB224		Christy		HB101.....		pFW32-285	spt3-285
FB226	pPM702	"MCB 4, 657-665; Maynard Olsen	contains an ochre suppressor	..........		pPM702	SUP4-o CEN3 amp TRP1 ARS1
FB227		"MCB 4, 657-665, Maynard Olsen	UGA suppressor	..........		pPM600	SUP4-UGA CEN3 ampR TRP1 ARS1
FB228		Patty and Fred	high copy number	HB101.....		pFW53	spt3::lacZ amp URA3
FB229		Patty and Fred	Deletion of the HpaI fragment from Ty917 from FB161 plasmid	HB101.....		pFW54	amp URA3 Ty917-HpaI deletion
FB230		Patty and Fred	Deletion of HpaI from Ty917(480)	HB101.....		pFW55	amp URA3 Ty917(480)HpaI deletion
FB231	GRF559	Giora Simchen	Partial eviction of lys2-61 to the EcoRI site	DB6507....		pGS4	amp URA3 lys2-61 (partial)
FB232	GRF560	Giora Simchen	Complete Ty61 (lys2-61) on this plasmid	DB6507....		pGS5	amp URA3 lys2-61
FB233		M. Casadaban	Plasmid originally in B60; put in HB101 for better plasmid preps	HB101.....		pMC1871	tet 'lacZ
FB234		Patty and Fred		HB101.....		pFW56	amp spt3::lacZ
FB235		Christy	subclone of LYS2 RI-H3 into YCp50	DB6507....		pCC2	amp URA3 LYS2 CEN
FB236	RR1	T.J. Koerner and A. Tzagaloff	Other markers not known; probably rec+. Host for pATH vectors for trpE fusi ons.	RR1.07....	r- m-
FB237	RR1delM15	Lothar	host for pUR290-2 plasmids	RR1delM15.	r- m- lacZalpha deletion
FB238		"J. Gorman, SmithKline	for promoter fusions to galK	E. coli...		pYSK7-54	amp TRP1 'galK CEN3
FB239		"J. Gorman, SmithKline	for protein fusions to galK	.. coli...		pYSK9-8	amp TRP1 galK CEN3
FB240		"J. Gorman, SmithKline	for protein fusions to galK	.. coli...		pYSK9-10	amp TRP1 'galK CEN3
FB241		"J. Gorman, SmithKline	for protein fusions to galK	.. coli...		pYSK9-12	amp TRP1 'galK CEN3
FB242		Patty and Fred	out of frame fusion SPT3-lacZ	RR1delM15.		pFW57	lacZ amp spt3'
FB243		Patty and Frecd	out of frame SPT3-lacZ fusion	RR1delM15.		pFW58	lacZ amp spt3'
FB244		Patty and Fred	"In frame fusion, SPT3-lacZ in pUR292 "	RR1delM15.		pFW59	amp SPT3-lacZ
FB245		Patty and Fred	"fusion to Bam linker at spt3-302, approx. 40 aa of SPT3 included "	HB101.M15.		pFW60	amp URA3 SPT3-lacZ
FB246		Patty and Fred	SPT3-lacZ with lacZ in wrong orientation	HB101.M15.		pFW61	amp URA3 'lacZ
FB247		Patty and Fred	SPT3 fusion at spt3-382; wrong orientation	HB101.M15.		pFW62	URA3 amp 'lacZ
FB248		Patty and Fred	fusion at spt3-382; lacZ in twice	HB101.M15.		pFW63	amp URA3 'lacZ SPT3'
FB249		Fred	deletion between spt3-302 and spt3-382	RR1delM15.		pFW64	URA3 amp spt3del
FB250		Fred	insert of 'lacZ into BglII of pSL42-2	RR1delM15.		pFW65	amp URA3 lys2::lacZ
FB251		Patty and Fred	URA3 in HindIII site of Ty917(480) clone (pFW52)	HB101.....		pFW66	amp Ty917(480)-URA3 URA3
FB252		Patty and Fred	subclone 'lacZ into spt3-382	HB101.....		pFW67	amp spt3-382::lacZ 2 micron
FB253		.	"EMBO 2, 1971-1794 (1983); for lacZ fusions for antibodies "	RR1delM15.	lacZdel r-	pUR290	ampR lacZ
FB254		"EMBO 2, 1791-1794 (1983)	for lacZ fusions for antibodies	RR1delM15.	lacZdel r-	pUR291	lacZ amp
FB255		"EMBO 2, 1971-1794 (1983)	for lacZ fusions for antibodies	RR1delM15.	lacZdel r-	pUR292	amp lacZ
FB256			out of frame	HB101.M15.		pFW68	amp URA3 lys2-128delta::lacZ
FB257		Fred	spt3-202 in YIp5	HB101.M15.		pFW69	amp URA3 spt3-202
FB258			RI 1.4 kb fragment contains TRP1 and ARS1 into RI site of pBR322	.B101.M15.		YRp7	TRP1 ars1 tet amp
FB259		Rose and Botstein	can be used for fusions	.B101.M15.		pRB45	LEU2 URA3 amp 2 micron 'lacZ
FB260			RI-Xho of SPT3	RR1delM15.		pFW42	amp SPT3
FB261		Fred	Fusion of Ty917 to lacZ	HB101.....		pFW70	amp URA3 Ty917-lacZ
FB262		Fred	"Ty917-lacZ, same as pFW70; different isolate "	HB101.....		pFW71	amp URA3 Ty917-lacZ
FB263		Fred	"Ty917-lacZ fusion; same as pFW70, 71 but different isolate "	HB101.....		pFW72	amp URA3 Ty917-lacZ
FB264		Fred	GAL-SPT3 fusion	HB101.....		pFW73	GAL-SPT3 URA3 2 micron
FB265		Fred	"GAL-SPT3 fusion, fused at spt3-354 (different from pFW73) "	HB101.....		pFW74	amp URA3 SPT3 GAL-SPT3
FB266		Fred	GAL-SPT3 fusion at spt3-374	HB101.....		pFW75	amp URA3 GAL-SPT3 2 micron
FB267		Fred	SPT3 is in the wrong orientation!!!	HB101.....		pFW76	amp URA3 GAL-SPT3 2 micron
FB268		BRL	a useful plasmid	JM83......	ara del(lac-pro) thi strA phi80dlacZ delM15	pUC18	amp
FB269		BRL	Another useful plasmid	JM83......	ara del(lac-pro) thi strA phi80dlacZ delM15	pUC19	amp
FB270		"Fred, Patty, and Christy	spt3-244 is an ochre mutation	HB101.....		pFW77	amp URA3 spt3-244
FB271		"Fred, Patty, and Christy	spt3-269(UGA)	HB101.....		pFW78	amp URA3 spt3-269(UGA)
FB272		"Fred, Christy, Patty	spt3-271(UAA)	HB101.....		pFW79	amp URA3 spt3-271(UAA)
FB273		? maybe Ben Hall lab	HindIII fragment of PYK1 in pBR322	HB101.....		pFR2	PYK1 amp
FB274	MM572	Martin Marinus	"dam, to make DNA cuttable by certain enzymes such as BclI "	GM237.....	dam-3 recA1 sin-1 thr-1 leuB6 proA2 metB1 thi1 deoB16 ara-14 lacY1 gal K2 rspL260 tsx33 supE44 mut4
FB275		Clare and Farabaugh	Ty-lacZ fusion	HB101.....		pJCdel44	"amp, 2 micron, Ty-lacZ
FB276		Clare and Farabaugh	Ty-lacZ fusion	HB101.....		pJCdel35	amp 2 micron Ty-lacZ
FB277		Clare and Farabaugh	Ty-lacZ fusion	HB101.....		pJCdel38	amp 2 micron URA3 Ty-lacZ fusion
FB278		Clare and Farabaugh	Ty-lacZ fusion	HB101.....		pJCdel36	amp URA3 2 micron Ty-lacZ
FB279		Clare and Farabaugh	Ty-lacZ fusion	HB101.....		pJCdel37	amp URA3 2 micron Ty-lacZ fusion
FB280		FW		FB274.....		pFW79	amp URA3 spt3-271(UAA)
FB281	JM107		streak on M9 glucose + B1 to hold F'	JM107.....	del(lac-pro) thi gyrA96 endA1 hsdR17 relA1 supE44 (F' traD36 proAB lac Iq lacZdelM15)	none
FB282	B731	Karen Durbin		FB1.7.....		YCp701	his4-912delta ura3 amp cen3 ars1
FB283	B924	Karen Durbin	AT to GC at basepair 166 of the delta; His+ at all temps	HB101.....		YCp701-1	his4-912deltaR amp ura3
FB284		R. Surosky	Beware of this plasmid. May be incorrect.	HB101.....			his4-912delta
FB285	TB1	BRL	For transformaing pUCclones into r-m+	TB1.1.....	ara del(lac pro) thi strA phi80dlacZ delM15 r-K m+K
FB286		Heffron	see notebook	RDP146....		pOX38::m-Tn3(lacZ)
FB287		Heffron	see notebook	RDP146....		pOX38::m-Tn3(URA3)
FB288		Heffron	see notebook	RDP146....		pOX38::m-Tn3(TRP1)
FB289		Heffron	see notebook	RDP146....		pOX38::m-Tn3(HIS3)
FB290		Heffron	see notebook	RDP146....		pOX38::m-Tn3(LEU2)
FB291		Heffron	see notebook	RDP146....		pOX38::m-Tn3
FB292		Heffron	see notebook	RDP146....		pOX38::M-Tn3leu2-lac
FB293		Heffron	see notebook	RDP146....		pOX38::m-Tn3his3-lac
FB294		Heffron	see notebook	RDP146....		pOX38::m-Tn3ura3-lac
FB295		Heffron	see notebook	RDP146....		pOX38::m-Tn3trp1-lac
FB296		Heffron	see notebook	RDP146....		pLB101
FB297		Heffron	see notebook	RDP145....		pHSS6
FB298		Heffron	see notebook	NS2114....
FB299		Kate	isolatae 1.2	FB285.....		pFW80	pUC18 912delta Rsa-Taq
FB300		Kate .	isolate 1.3	FB285.....		pFW80	pUC18 912 delta Rsa-Taq
FB301		Kate	isolate 1.4	FB285.....		pFW80	pUC18 912delta Rsa-Taq
FB302		Fred	lacZ in spt3-334	HB101.....		pFW81	amp URA3 spt3::lacZ
FB303		Kate	"from pJCdel44, integrating version "	FB1.1.....		pFW82	amp ura3 Ty-lacZ
FB304		Kate	"from pJCdel44, integrating version "	FB1.1.....		pFW83	amp ura3 Ty-lacZ
FB305		Kate	Ty from pJCdel35; integrating version	FB1.1.....		pFW84	amp ura3 Ty-lacZ
FB306		Kate	from pJCdel35; integrating version	FB1.1.....		pFW85	amp ura Ty-lacZ
FB307		Kate	from pJCdel37; integrating version	FB1.1.....		pFW86	amp ura3 Ty-lacZ
FB308		Kate	from pJCdel37; integrating version	FB1.1.....		pFW87	amp ura3 Ty-lacZ
FB309		Promega	see notebook	HB101.....		pGEM1
FB310		Promega	see notebook	HB101.....		pGEM2
FB311	B661	"J. Bact. 156, 625	"pBR325 derivative, leu2-d for high copy number, in G.R. Fink collection "	unknown...		pMP78-1	leu2-d amp 2 micron camR
FB315		L. Guarente	"Guarente and Ptashne, Cell (1980); for fusions "	HB101.n...		pLGdel312	amp URA3 'lacIZ 2 micron
fb312		Mark Rose	KAR1 probe; see notebook	HB101.n...		pMR95	KAR1 ampR
FB313		Susan Michaelis	MFa2 probe; published in MCB	HB101.n...		pSM29	MFa2 ampR
FB314		Susan Michaelis	MFa1 probe; published in MCB	HB101.n...		pSM39	MFa1 ampR
FB316		L. Guarente	"Guarente et al., Cell, Feb. 1984; CEN vector for fusions "	HB101.n...		pBL101	ampR URA3 'lacIZ CEN4 ARS1
FB317		D. Garfinkel	high copy number suppressor	HB101.n...		CS14	ampR; high copy SPT23
FB318	B163	R. Surosky and G.R. Fink	see notebook for exact map of fragment	?.101.n...		B163	ampR Ty S13 BglII fragment
FB319		D. Botstein	"see Ng and Abelson, PNAS 77,3912 (1980) "	..101.n...		pRB149	amp tet URA3
FB320		Jodi Hirschman	For making SPT3 in vitro; SPT3 Sma-EcoRI under SP6 control	HB101.n...	amp SP6-SPT3	pJEH1	SPT3 transcription under SP6 control in pGEM1
FB321		Jodi	spt3-101 under SP6 control	HB101.....		pJEH2	amp SP6-spt3-101
FB322		Jodi	SPT3 under T7 control	HB101.....		pJEH3	amp T7-SPT3
FB323		Jodi	spt3-101 under T7 control	HB101.....		pJEH4	amp T7-spt3-101
FB324		Jodi	His+ derivative of YCp701	HB101.....		YCp701-R5	amp his4-912dR5 URA3
FB325		Jodi	His+ derivative of YCp701	HB101.....		YCP701-R58	amp URA his4-912dR58
FB326	MC1065	M. Casadaban	can use to check yeast TRP1	MC1065....	F- galE15 galK16 dellacX74 (pro+) leuB6 trpC9830 strA(rspL) B1-
FB327	B80	R. Davis via G. Fink	"R1 fragment with Ty and delta. Cell 16, 739 (1979) "	.C1065....		S13	amp Ty
FB328	B1056	F. Nagawa and G. Fink	URA3 with XhoI ends	HB101.....		pFN35	amp URA3 with XhoI ends
FB329		Rick Gaber	Replaced tet region of YIp5 with pUC18 polylinker	HB101.....		pRG331	amp URA3 pUC polylinker
FB330		Rick Gaber	"WARNING!!- At low frequency, the plasmid loops out between the ori and polyl inker. tet region of YCp50 replaced with pUC18 polylinker "	HB101.....		pRG332	amp URA3 CEN4 ARS1 pUC18 polylinker
FB331		Jodi	His+ revertant of his4-912d	HB101.....		YCp701-R177	URA CEN4 ARS1 his4-912dR177
FB332	MC1061	Dan Weeks	good for transformations; also entered earlier in the collection	MC1061....		none
FB333		T.J. Koerner and Tzagaloff	see notebook; for fusions	HB101.....		pATH1	amp trpE
FB334		T.J. Koerner and Tzagaloff	see notebook; for fusions	HB101.....		pATH2	amp trpE
FB335		T.J. Koerner and Tzagaloff	see notebook; for fusions	HB101.....		pATH3	amp trpE
FB336		Kate and Fred	in pATH1; fusion at spt3-302 BamHI linker	MC1061....		pFW88	URA3 trpE-SPT3
FB337		Kate and Fred	fusion at spt3-302 in pATH1	MC1061....		pFW89	amp trpE-SPT3
FB338		Kate and Fred	fusion in pATH2; at spt3-302	MC1061....		pFW90	amp trpE-SPT3
FB339		Kate and Fred	fusion in pATH2 at spt3-302	MC1061....		pFW91	amp trpE-SPT3
FB340		Kate	original clone candidate	HB101.....		pFW92	amp URA CEN SPT8
FB341		Kate	original SPT8 clone candidate	HB101.....		pFW93	amp URA3 CEN4 ARS1 SPT8
FB342		Kate	original SPT8 clone candidate	HB101.....		pFW94	amp URA3 CEN4 ARS1 SPT8
FB343		Kate	original SPT8 clone candidate	HB101.....		pFW95	amp URA3 CEN4 ARS1 SPT8
FB344		Kate	original SPT8 clone candidate	HB101.....		pFW96	amp URA3 CEN4 ARS1 SPT8
FB345		Kate	pATH1 vector; in frame trpE-SPT3 fusion	RR1.......		pFW88	trpE-SPT3 amp
FB346		Kate	"pATH1 vector, trpE-SPT3 in frame fusion "	RR1.......		pFW89	trpE-SPT3
FB347		Kate	fusion in pATH2	RR1.......		pFW90	amp trpE-SPT3 out of frame
FB348		Kate	in pATH2	RR1.......		pFW91	amp trpE-SPT3 out of frame
FB349		Fred	EcoRV-BglII of lys2-128d in pUC18	TB1.......		pFW97	amp lys2-128d 5' end
FB350		Fred	EcoRV-BglII of lys2-128d in pUC19	TB1.......		pFW98	amp lys2-128d 5' end
FB351	LM1035	Connie Cepko	derivative of HB101; supposed good transforming strain	LM1035....	same as HB101
FB352	AR68	SmithKline	good for production of hybrid proteins; see notebook	AR68.5....
FB353		D. Botstein	SUC2 probe	HB101.....		pRB59	amp SUC2 internal fragment
FB354		Kate	original SPT8 clone	HB101.....		pFW99	YCp50 SPT8
FB355		Kate	original SPT8 clone	HB101.....		pFW100	YCp50 SPT8
FB356		Kate	original SPT8 clone	HB101.....		pFW101	YCp50 SPT8
FB357		Kate	original SPT8 clone	HB101.....		pFW102	YCp50 SPT8
FB358		Kate	original SPT8 clone	HB101.....		pFW103	YCp50 SPT8
FB359		Kate	original clone	HB101.....		pFW104	YCp50 SPT7
FB360		Kate	original clone	HB101.....		pFW105	YCp50 SPT7
FB361		Kate	original clone	HB101.....		pFW106	YCp50 SPT7
FB362		Kate	original clone	HB101.....		pFW107	YCp50 SPT7
FB363		Kate	original clone	HB101.....		pFW108	YCp50 SPT7
FB364		Kate	original clone	HB101.....		pFW109	YCp50 SPT7
FB365		Kate	original clone	HB101.....		pFW110	YCp50 SPT7
FB366		Rick Gaber	same as in FB330 with unknown difference	HB101.....		pRG377	YCp50 pUC18 polylinker
FB367	B987	"PNAS 82, 8557	"small deletion, Nagawa and Fink "	.B101.....		x-99	HIS4-lacZ cen ura ars1 Xho linker
FB368	B994	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-407	HIS4-lacZ amp cen4 ars1 ura3 Xho linker
FB369	B1005	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-101	HIS4-lacZ cen4 ars1 ura3 Xho linker
FB370	B1006	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-122	HIS4-lacZ cen4 ars1 ura3 Xho linker
FB371	B1012	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-7	HIS4-lacZ cen4 ura3 ars1 Xho linker
FB372	B1015	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-198	HIS4-lacZ cen4 ura3 ars1 Xho linker
FB373	B1019	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-52	HIS4-lacZ cen4 ars1 ura3 Xho linker
FB374		Kate	Cla-Sal subclone	MC1061....		pFW111	amp SPT7 YCp50
FB375		Fred	LYS2 5' probe	MC1061....		pFW112	amp LYS2 RI-BglII 5' fragment
FB376		F. del Rey and G.R. Fink	RsaI fragment of sigma plasmid into ClaI of YIp5	.C1061....		pML52	URA3 sigma
FB377		Scott Emr	vector for lacZ fusions	MC1061....		pSEY101	ura3 lacZ 2 micron amp
FB378		Scott Emr	CEN vector for lacZ fusions	MC1061....		pSEYC102	ura3 ars1 cen4 amp lacZ
FB379		Scott Emr	for lacZ fusions	FB383.....		pSEY8	amp 2 micron lacZ