MYL3 exons, primers, and amplimers

Exons, Primers, and Amplimers of cardiac Essential Myosin Light Chain

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | top

MYL3 Exon 1

CTGCTTTCTGCATTCTTCTCTCCACATCCCTCTCTGTACTTACAGCCCCC
AATGGCCCCCAAAAAGCCAGAGCCCAAGAAGGATGATGCCAAGGCAGCCC
CCAAGGCAGCTCCAGCTCCCGCACCTCCCCCTGAGCCTGAGCGCCCTAAG
GAGGTCGAGTTTGATGCTTCCAAGATCAAG

length: 180 bp

EXON	from	to
genomic DNA	1001	1180
mRNA

Primers for Exon 1
1F tttatggccctgtccctattgtgc (24 bp)

1R ctcctgctgcagcccttctgatg (23 bp)

**Primers for Exon 1**
1F	tttatggccctgtccctattgtgc	(24 bp)
1R	ctcctgctgcagcccttctgatg	(23 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 1

TTTATGGCCCTGTCCCTATTGTGCACTGCAGGGGTGGGGCTGGGGTCATG
AGGTATCCGGGCAGGATAAAGGCCTGGGTGAGGCGGCTCACCTACCCTGC
TTTCTGCATTCTTCTCTCCACATCCCTCTCTGTACTTACAGCCCCCAATG
GCCCCCAAAAAGCCAGAGCCCAAGAAGGATGATGCCAAGGCAGCCCCCAA
GGCAGCTCCAGCTCCCGCACCTCCCCCTGAGCCTGAGCGCCCTAAGGAGG
TCGAGTTTGATGCTTCCAAGATCAAGGTGGGTATGGAAGCTGGGTGGTGG
CAATAGGAGCAGGGCAAGTTAGTGGATCCCAGAGTGGACCTAGCAAGAGG
CAGGTCAGGCTCTGGAGGTGAGCACTGAGCTGACGCTGGAAGCTTCTGTG
CAGAGGTCAGACAAGCCCTGACTGCTTCAGGCATCAGAAGGGCTGCAGCA
GGAG

length: 454 bp

AMPLIMER	from	to
genomic DNA	905	1358

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | top

MYL3 Exon 2

ATTGAGTTCACACCTGAGCAGATTGAAG

length: 28 bp

EXON	from	to
genomic DNA	3455	3482
mRNA

Primers for Exon 2
2F tgctggaatgggacaggctgagac (24 bp)

2R acatccccacactgcccgtaggt (23 bp)

**Primers for Exon 2**
2F	tgctggaatgggacaggctgagac	(24 bp)
2R	acatccccacactgcccgtaggt	(23 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 2

TGCTGGAATGGGACAGGCTGAGACAGTTGACATTCTGGGCTGGCCACCGA
GTGCCCAGGAGGAGGTGTGGCCCCACCTTTTAAGCCGGGCATCTGAGTCT
TTGGGGTCTCTTTTCAGATTGAGTTCACACCTGAGCAGATTGAAGGTGAG
CAGGGGTCTTGAACCCAGGGGTTGCAGGATTGGGCTGGGGTTCCCTGGTC
TGAGGGTCCTGCCAGGGGTGTGGGGAGTGGGGTAGGCTGACCCTGGGAGT
GTCTGTGGCTCACTGCCCTCTCCTGGCAGAGTTCAAGGAAGCCTTCATGC
TGTTCGACCGCACACCCAAGTGTGAGATGAAGATCACCTACGGGCAGTGT
GGGGATGT

length: 358 bp

AMPLIMER	from	to
genomic DNA	3338	3695

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | top

MYL3 Exon 3

AGTTCAAGGAAGCCTTCATGCTGTTCGACCGCACACCCAAGTGTGAGATG
AAGATCACCTACGGGCAGTGTGGGGATGTCCTGCGGGCGCTGGGCCAGAA
CCCCACACAGGCAGAAGTGCTCCGTGTCCTGGGGAAGCCAAGACAGGAAG

length: 150 bp

EXON	from	to
genomic DNA	3617	3766
mRNA

Primers for Exon 3
3F gggctggggttccctggtctg (21 bp)

3R ccctcatcgggacaatgcgagat (23 bp)

**Primers for Exon 3**
3F	gggctggggttccctggtctg	(21 bp)
3R	ccctcatcgggacaatgcgagat	(23 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 3

GGGCTGGGGTTCCCTGGTCTGAGGGTCCTGCCAGGGGTGTGGGGAGTGGG
GTAGGCTGACCCTGGGAGTGTCTGTGGCTCACTGCCCTCTCCTGGCAGAG
TTCAAGGAAGCCTTCATGCTGTTCGACCGCACACCCAAGTGTGAGATGAA
GATCACCTACGGGCAGTGTGGGGATGTCCTGCGGGCGCTGGGCCAGAACC
CCACACAGGCAGAAGTGCTCCGTGTCCTGGGGAAGCCAAGACAGGAAGGT
AGTGCACCTGCCCGAGAGCCCCCATAGTGTTAGTCCCGGGGAGACTGGCT
GGGTGGGCTGCCATCCATCCTGCGGGATAGCACGAGAATCTTTCCTGACA
TCTCGCATTGTCCCGATGAGGG

length: 372 bp

AMPLIMER	from	to
genomic DNA	3519	3890

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | top

MYL3 Exon 4

AGCTCAATACCAAGATGATGGACTTTGAAACTTTCCTGCCTATGCTCCAG
CACATTTCCAAGAACAAGGACACAGGCACCTATGAGGACTTCGTGGAGGG
GCTGCGGGTCTTCGACAAGGAGGGCAATGGCACTGTCATGGGTGCTGAGC
TTCGCCACGTGCTGGCCACGCTGG

length: 174 bp

EXON	from	to
genomic DNA	4794	4967
mRNA

Primers for Exon 4
4F aaagtgcctcgcgatggtagtttg (24 bp)

4R gtctgccattgaggctccctaatt (24 bp)

**Primers for Exon 4**
4F	aaagtgcctcgcgatggtagtttg	(24 bp)
4R	gtctgccattgaggctccctaatt	(24 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 4

AAAGTGCCTCGCGATGGTAGTTTGCTTTTAAATGGCAAACTGGTGTAGAC
TGCTGTGAGAACTGGTGTAGACTGTTGTAGAACTGTGTGAGAGGTGGGGA
TAGCTCCTCATTATCAGGCATGGGGGAGGGGTGTGCCCACCCCAGCCTTA
GACCCTGGAACCTGGGACCATTTCTCTGCAGAGCTCAATACCAAGATGAT
GGACTTTGAAACTTTCCTGCCTATGCTCCAGCACATTTCCAAGAACAAGG
ACACAGGCACCTATGAGGACTTCGTGGAGGGGCTGCGGGTCTTCGACAAG
GAGGGCAATGGCACTGTCATGGGTGCTGAGCTTCGCCACGTGCTGGCCAC
GCTGGGTGAGGGCAGCCTCCTCCCCTACCCCAACTCCTTCCAGGGACATC
CTGGGAGAAACCACTCTGTTGCCCCCCAGGCCTGTGACCCATTGGTGAAA
TGGAGAGAGTTACATAATTAGGGAGCCTCAATGGCAGAC

length: 489 bp

AMPLIMER	from	to
genomic DNA	4613	5101

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | top

MYL3 Exon 5

GTGAGAGGCTGACAGAAGACGAAGTGGAGAAGTTGATGGCTGGGCAAGAG
GACTCCAATGGCTGCATCAACTATGAAG

length: 78 bp

EXON	from	to
genomic DNA	5981	6058
mRNA

Primers for Exon 5
5F cctggcctcagaaatggggtgatg (24 bp)

5R acctgggcacgaggtttagctggac (25 bp)

**Primers for Exon 5**
5F	cctggcctcagaaatggggtgatg	(24 bp)
5R	acctgggcacgaggtttagctggac	(25 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 5

CCTGGCCTCAGAAATGGGGTGATGGCCAAGGTCTTGTGAACAGTGCTGGT
TGTGGGAATACTGGAGAGGTTGAGCCACCATCTAGAGGGATTCAGTGGGT
GCCCCCACCCCATCATGCCTCTGCACGCACGGTGCTGACTCAGCCTCCCA
CTCCTGCCAGGTGAGAGGCTGACAGAAGACGAAGTGGAGAAGTTGATGGC
TGGGCAAGAGGACTCCAATGGCTGCATCAACTATGAAGGTGGGCTCAGCA
GGGGCAGGGGTCTTCTGGGAGGGGAGTGCTGGGACCCAGGCTCCCAGCCA
TGGCTTCCCCATGTTGCACTCCTGCCACTCACTCTCCTCTTCTTTCCAGC
ATTTGTGAAGCACATCATGTCCAGCTAAACCTCGTGCCCAGGT

length: 393 bp

AMPLIMER	from	to
genomic DNA	5821	6213

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | top

MYL3 Exon 6

CATTTGTGAAGCACATCATGTCCAGCTAAACCTCGTGCCCAG

length: 42 bp

EXON	from	to
genomic DNA	6170	6211
mRNA

Primers for Exon 6
6F gaggctgacagaagacgaagtgga (24 bp)

6R agacggaggcagcaggatgtcaag (24 bp)

**Primers for Exon 6**
6F	gaggctgacagaagacgaagtgga	(24 bp)
6R	agacggaggcagcaggatgtcaag	(24 bp)

(Annealing temp.: 62° C.)

Amplimer for Exon 6

GAGGCTGACAGAAGACGAAGTGGAGAAGTTGATGGCTGGGCAAGAGGACT
CCAATGGCTGCATCAACTATGAAGGTGGGCTCAGCAGGGGCAGGGGTCTT
CTGGGAGGGGAGTGCTGGGACCCAGGCTCCCAGCCATGGCTTCCCCATGT
TGCACTCCTGCCACTCACTCTCCTCTTCTTTCCAGCATTTGTGAAGCACA
TCATGTCCAGCTAAACCTCGTGCCCAGGTAGGCACCCACTCCCTCCTGAA
CCCGCTGCTGCCACCTGCCCACACTCCACCAGCTGCAGTGCTCACTTGAC
ATCCTGCTGCCTCCGTCT

length: 318 bp

AMPLIMER	from	to
genomic DNA	5985	6302

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | top

MYL3 Exon 7

GGAAGGCTGTGCAGGGACGTCTCATCTCCCATGTGTGATGCTGACACCAG
CGGCCTGGAGTCGTGGGAAGGAGGGGAGCCTTACCAAGACTCCTGCAAAA
ACCCTTGGACCTCTCCACGTGGTTGCCGTCTCTGGCCCCTCTCAGGCTGT
GCTTACCTGTGACAGCAGCTCTGTCCCCACCCCATGGGCCTCATGAATAA
ATGGCTTCTTGCCGGC

length: 216 bp

EXON	from	to
genomic DNA	6350	6565
mRNA

Primers for Exon 7
( bp)

( bp)

(Annealing temp.: ° C.)

Amplimer for Exon 7

length: bp

AMPLIMER	from	to
genomic DNA

Jump to exon 1 | 2 | 3 | 4 | 5 | 6 | 7 | top

citations & disclaimer | contact us

Last modified: April 24, 2006