MYL2 exons, primers, and amplimers

Exons, Primers, and Amplimers of cardiac Regulatory Myosin Light Chain

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MYL2 Exon 1

agagctgggcggagtgtggaattcttgtcgggaggcagtgctgggtcctt
tccaccatg

length: 59 bp

EXON	from	to
genomic DNA	96	154
mRNA

Primers for Exon 1
1F gtgcccgcctcacctatgact (21 bp)

1R ggggcattgattggggttcct (21 bp)

**Primers for Exon 1**
1F	gtgcccgcctcacctatgact	(21 bp)
1R	ggggcattgattggggttcct	(21 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 1

GTGCCCGCCTCACCTATGACTGCCAAAAGCGGTCATGGGGTTATTTTTAA
ACATGGGGAGGAAGTATTTATTGTTCCTGGGCTGCAGAGAGCTGGGCGGA
GTGTGGAATTCTTCTCGGGAGGCAGTGCTGGGTCCTTTCCACCATGGTGA
GTACAAGGGCTCCAGGAGGTGATGATGCCGGGTGGGCGAGGAGAGGAAGC
CACTACAAGCGAGGGACCACGGCGGAGGGGGAGGAAGGCGGCCCTCAAAG
CCTCCAGCCCCTTGTGGAAAAAGCCCCATTGACTGCGCGGGCACAGAGGA
ACCCCAATCAATGCCCC

length: 317 bp

AMPLIMER	from	to
genomic DNA	9	325

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MYL2 Exon 2

gcacctaagaaagcaaagaagagagccgggggcgccaactccaacgtgtt
ctccatgttcgaacagacccaaatccaggaatttaaggag

length: 90 bp

EXON	from	to
genomic DNA	1488	1577
mRNA

Primers for Exon 2
2F cacccagagtaggggcctgaccta (24 bp)

2R ttcaggccgaatttgggattgttt (24 bp)

**Primers for Exon 2**
2F	cacccagagtaggggcctgaccta	(24 bp)
2R	ttcaggccgaatttgggattgttt	(24 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 2

CACCCAGAGTAGGGGCCTGACCTAGTTTTTTGACTCCCAGCCTCTCACTC
TTTTAATCGATGCTTTTTTCCCCCAGGCACCTAAGAAAGCAAAGAAGAGA
GCCGGGGGCGCCAACTCCAACGTGTTCTCCATGTTCGAACAGACCCAAAT
CCAGGAATTTAAGGAGGTGGGTGCAGCTATTGAATCATCCGCCTGGATGG
AAATCCCACCCACGAGGGAGAACCTTCTTTTTGTTCCATATTCCCAGCAG
GGACCTTTCATTAGATGCCTCTATCCTCCAAACAATCCCAAATTCGGCCT
GAA

length: 303 bp

AMPLIMER	from	to
genomic DNA	1412	1714

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MYL2 Exon 3

gccttcactatcatggaccagaacagggatggcttcattgacaagaacga
tctgagagacacctttgctgcccttg

length: 76 bp

EXON	from	to
genomic DNA	4891	4966
mRNA

Primers for Exon 3
3F ggcctgacgttttgaagaaactgt (24 bp)

3R tcccatcccctgaaatttgtctta (24 bp)

**Primers for Exon 3**
3F	ggcctgacgttttgaagaaactgt	(24 bp)
3R	tcccatcccctgaaatttgtctta	(24 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 3

GGCCTGACGTTTTGAAGAAACTGTTGGATTATCTTCAATTGTTGACTTTT
AATCTGAGGTCCTACAATGATCTTTGAAGATCCCAGAATCCACTCCTGCC
AAGTCCTTGGCCCAATCTCTTGGGCAACTCTGCCAGTTTCTCCCAGGCTG
AGCTGCCAATCACTTCCCTTCCACAGGCCTTCACTATCATGGACCAGAAC
AGGGATGGCTTCATTGACAAGAACGATCTGAGAGACACCTTTGCTGCCCT
TGGTATGTCACCCTCTCTAGCCCTGCATGAGGGTCTCTTTATCTCACATC
CATGAGGAGCAGGAGGTGGGACCATCAGAAAAGCTCAAGATGGTGTCATT
TCCCAAAGAAACAACTCTGCCACAACTGGTCTTTAAGACAAATTTCAGGG
GATGGGA

length: 407 bp

AMPLIMER	from	to
genomic DNA	4715	5121

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MYL2 Exon 4

ggcgagtgaacgtgaaaaatgaagaaattgatgaaatgatcaaggaggct
ccgggtccaattaactttactgtgttcctcacaatgtttggggagaaact
taagg

length: 105 bp

EXON	from	to
genomic DNA	6391	6495
mRNA

Primers for Exon 4
4F ggagccaatggcagtgtcataatg (24 bp)

4R cgcccagccaagttgctgttt (21 bp)

**Primers for Exon 4**
4F	ggagccaatggcagtgtcataatg	(24 bp)
4R	cgcccagccaagttgctgttt	(21 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 4

GGAGCCAATGGCAGTGTCATAATGGTTCTTTCTCCCCAGCTCGGAGTCCC
TGAGTGTGTGTTTCCTACCCTAGGGCGAGTGAACGTGAAAAATGAAGAAA
TTGATGAAATGATCAAGGAGGCTCCGGGTCCAATTAACTTTACTGTGTTC
CTCACAATGTTTGGGGAGAAACTTAAGGGTAAGGTCGTGTGTGTGTGTGT
GTGTCTGTGTGTATGTGTCTATGTTTCTTCGGGGGGGCTGGCAGAAGATG
GTTAACTTTAAAAGAGATAAAGCACCATTGGAAAAAAATAATGAAAACAG
CAACTTGGCTGGGCG

length: 315 bp

AMPLIMER	from	to
genomic DNA	6318	6632

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MYL2 Exon 5

gagcggaccctgaggaaaccattctcaacgcattcaaagtgtttgaccct
gaaggcaaaggggtgctgaaggctgatta

length: 79 bp

EXON	from	to
genomic DNA	7357	7435
mRNA

Primers for Exon 5
5&6F agccttgctctggccccttcatc (23 bp)

5&6R tgtggggtcaggggtgctttagac (24 bp)

**Primers for Exon 5**
5&6F	agccttgctctggccccttcatc	(23 bp)
5&6R	tgtggggtcaggggtgctttagac	(24 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 5

AGCCTTGCTCTGGCCCCTTCATCTCTGGGGGAACTTGGGCCCTGCTGCCT
GGGGTGCCAGTGGTTCCCAGCCACCCCCAGTACATGTAAGCCCTGTGTTC
CGTTTCAGGAGCGGACCCTGAGGAAACCATTCTCAACGCATTCAAAGTGT
TTGACCCTGAAGGCAAAGGGGTGCTGAAGGCTGATTAGTGAGTGGGGTCT
GTGGGGGAGGAAGGGGGTTCCCTGCTTGCCCCCTGTCCCCCCTACACACA
CACAACTGACACCAACACCTGCTTTCCTTTCTCTGCAGCGTTCGGGAAAT
GCTGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGAGGTAATGGGGTCCT
TCAAGCACTCCCTCTAACCCAGCTCCTGGGTTCTCCTTCTGGGGGGCTCC
GTCTCCCCTCTCGTCTAAAGCACCCCTGACCCCACA

length: 436 bp

AMPLIMER	from	to
genomic DNA	7249	7684

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MYL2 Exon 6

cgttcgggaaatgctgaccacgcaggcggagaggttttccaaggaggag

length: 49 bp

EXON	from	to
genomic DNA	7537	7585
mRNA

Primers for Exon 6
5&6F agccttgctctggccccttcatc (23 bp)

5&6R tgtggggtcaggggtgctttagac (24 bp)

**Primers for Exon 6**
5&6F	agccttgctctggccccttcatc	(23 bp)
5&6R	tgtggggtcaggggtgctttagac	(24 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 6

AGCCTTGCTCTGGCCCCTTCATCTCTGGGGGAACTTGGGCCCTGCTGCCT
GGGGTGCCAGTGGTTCCCAGCCACCCCCAGTACATGTAAGCCCTGTGTTC
CGTTTCAGGAGCGGACCCTGAGGAAACCATTCTCAACGCATTCAAAGTGT
TTGACCCTGAAGGCAAAGGGGTGCTGAAGGCTGATTAGTGAGTGGGGTCT
GTGGGGGAGGAAGGGGGTTCCCTGCTTGCCCCCTGTCCCCCCTACACACA
CACAACTGACACCAACACCTGCTTTCCTTTCTCTGCAGCGTTCGGGAAAT
GCTGACCACGCAGGCGGAGAGGTTTTCCAAGGAGGAGGTAATGGGGTCCT
TCAAGCACTCCCTCTAACCCAGCTCCTGGGTTCTCCTTCTGGGGGGCTCC
GTCTCCCCTCTCGTCTAAAGCACCCCTGACCCCACA

length: 436 bp

AMPLIMER	from	to
genomic DNA	7249	7684

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MYL2 Exon 7

gttgaccagatgttcgccgccttcccccctgacgtgactggcaacttgga
ctacaagaacctggtgcacatcatcacccacggagaagagaaggactagG
AGGGGGCTCGCTGCTGCGCCCTGGGCTCGTCTTTGCAGAGTGGTCCCTGC
CCTCATCTCTCTCCCCCGAGTACCGCCTCTGTCCCTACCTTGTCTGTTAG
CCATGTGGCTGCCCCATTTATCCACCTCCATCTTCTTTGCAGCCTGGGTG
GCTATGGGTACTTCGTGGCCGCACATCCTACAGTTGGAAATCCATCCAGA
GGCCATGTTCCAATAAACAGGAGGTCGTGTATTTGGTCACGACATTTCTC
TGA

length: 353 bp

EXON	from	to
genomic DNA	9506	9858
mRNA

Primers for Exon 7
7F agatggccgtgcctttatac (20 bp)

7R gaaacagttctgagtggctg (20 bp)

**Primers for Exon 7**
7F	agatggccgtgcctttatac	(20 bp)
7R	gaaacagttctgagtggctg	(20 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 7

AGATGGCCGTGCCTTTATACCCCCACACCAATCCCATCCGCTGGCTGTGG
GCTGCCCCAGAAAGGGCATGACCTTGGGCGAGGGGGCCCTGCAGCAGCTG
GGGAAGTCCTTCCCCGGAGGACCCTCTGAGTGGCCCATGTTCCCATCCCC
CACCCCCCAGCTATCCGTCCACTCAGGCCCCGCCCACAGCCCCCCACCCT
CCGTCTCAGTTCCCCTCCCCTCGTCCTTAGCACGTGTTGCTGGCTCATTG
CAGGTTGACCAGATGTTCGCCGCCTTCCCCCCTGACGTGACTGGCAACTT
GGACTACAAGAACCTGGTGCACATCATCACCCACGGAGAAGAGAAGGACT
AGGAGGGGGCTCGCTGCTGCGCCCTGGGCTCGTCTTTGCAGAGTGGTCCC
TGCCCTCATCTCTCTCCCCCGAGTACCGCCTCTGTCCCTACCTTGTCTGT
TAGCCATGTGGCTGCCCCATTTATCCACCTCCATCTTCTTTGCAGCCTGG
GTGGCTATGGGTACTTCGTGGCCGCACATCCTACAGTTGGAAATCCATCC
AGAGGCCATGTTCCAATAAACAGGAGGTCGTGTATTTGGTCACGACATTT
CTCTGACAAACAGTGCCTTGTGTAAGGAAGGCAAAGGCAGATGAGCTAGT
GACAGCCTTGCAGTCATTATTATTGAATCTTGGTGACTCTTTATTTAAGG
TAGACCTTTGCAGCCACTCAGAACTGTTTC

length: 730 bp

AMPLIMER	from	to
genomic DNA	9253	9982

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Last modified: April 24, 2006