ACTC exons, primers, and amplimers

Exons, Primers, and Amplimers of cardiac alpha-Actin

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ACTC Exon 1

ATGTGTGACGACGAGGAGACCACCGCCCTGGTGTGCGACAACGGCTCTGG
GCTGGTGAAGGCCGGCTTTGCGGGCGATGACGCGCCCCGCGCTGTCTTCC
CGTCCATCGTGGGCCGCCCGCGGCACCAG

length: 129 bp

EXON	from	to
genomic DNA	1001	1129
mRNA

Primers for Exon 1
1F tgcagaaaccccctgaagc (19 bp)

1R gccatttcctagatcgctgga (21 bp)

**Primers for Exon 1**
1F	tgcagaaaccccctgaagc	(19 bp)
1R	gccatttcctagatcgctgga	(21 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 1

TGCAGAACCCCCTGAAGCTGTGCCAAGATGTGTGACGACGAGGAGACCAC
CGCCCTGGTGTGCGACAACGGCTCTGGGCTGGTGAAGGCCGGCTTTGCGG
GCGATGACGCGCCCCGCGCTGTCTTCCCGTCCATCGTGGGCCGCCCGCGG
CACCAGGTAAACTTCCCGCCGAGCCCCCCGTCCCACTCGGGACCCCTTCA
GTCCAGCGATCTAGGAAATGGC

length: 222 bp

AMPLIMER	from	to
genomic DNA	974	1195

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ACTC Exon 2

GGAGTTATGGTGGGTATGGGTCAGAAGGACTCCTACGTAGGTGATGAAGC
CCAGAGCAAGAGAGGCATCCTGACCCTGAAGTATCCCATCGAGCATGGTA
TCATCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTAC
AATGAGCTCCGTGTGGCTCCCGAGGAGCACCCCACCCTGCTCACAGAGGC
CCCGCTGAACCCCAAGGCCAACCGGGAGAAGATGACTCAGATCATGTTTG
AGACCTTCAATGTCCCTGCCATGTACGTGGCCATCCAGGCAGTGCTATCC
CTGTATGCTTCTGGCCGTACCACAG

length: 325 bp

EXON	from	to
genomic DNA	2240	2564
mRNA

Primers for Exon 2
2F ttcctgacatggtgagagca (20 bp)

2R tcggtgacttgggaatgtg (19 bp)

**Primers for Exon 2**
2F	ttcctgacatggtgagagca	(20 bp)
2R	tcggtgacttgggaatgtg	(19 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 2

TTCCTGACATGGTGAGAGCATGATTTTCTCATTTTTTCTTCTCATAGGGA
GTTATGGTGGGTATGGGTCAGAAGGACTCCTACGTAGGTGATGAAGCCCA
GAGCAAGAGAGGCATCCTGACCCTGAAGTATCCCATCGAGCATGGTATCA
TCACCAACTGGGACGACATGGAGAAGATCTGGCACCACACCTTCTACAAT
GAGCTCCGTGTGGCTCCCGAGGAGCACCCCACCCTGCTCACAGAGGCCCC
GCTGAACCCCAAGGCCAACCGGGAGAAGATGACTCAGATCATGTTTGAGA
CCTTCAATGTCCCTGCCATGTACGTGGCCATCCAGGCAGTGCTATCCCTG
TATGCTTCTGGCCGTACCACAGGTATGCTGGGCTCTGGGGACAGTTACTG
ATGAATCACATTCCCAAGTCACCGA

length: 425 bp

AMPLIMER	from	to
genomic DNA	2193	2617

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ACTC Exon 3

GCATTGTTCTGGACTCTGGGGATGGTGTAACTCACAATGTCCCCATCTAT
GAGGGCTACGCTTTGCCCCATGCCATCATGCGTCTGGATCTGGCTGGTCG
GGACCTCACTGACTACCTCATGAAGATCCTCACTGAGCGTGGCTACTCCT
TTGTCACCACTG

length: 162 bp

EXON	from	to
genomic DNA	3240	3401
mRNA

Primers for Exon 3
3F gagcagtggtgttgtcctcag (21 bp)

3R aagcagacccacactgtgg (19 bp)

**Primers for Exon 3**
3F	gagcagtggtgttgtcctcag	(21 bp)
3R	aagcagacccacactgtgg	(19 bp)

(Annealing temp.: 60° C.)

Amplimer for Exon 3

GAGCAGTGGTGTTGTCCTCAGGAATTTACCTTGTTCTTGTCTACTTCCCC
GGGCAGGCATTGTTCTGGACTCTGGGGATGGTGTAACTCACAATGTCCCC
ATCTATGAGGGCTACGCTTTGCCCCATGCCATCATGCGTCTGGATCTGGC
TGGTCGGGACCTCACTGACTACCTCATGAAGATCCTCACTGAGCGTGGCT
ACTCCTTTGTCACCACTGGTGAGTGTGTGTGTCTCATCTGCCACAGTGTG
GGTCTGCTT

length: 259 bp

AMPLIMER	from	to
genomic DNA	3184	3442

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ACTC Exon 4

CTGAACGTGAAATTGTCCGTGACATTAAAGAGAAGCTGTGCTATGTCGCC
CTGGATTTTGAGAATGAGATGGCCACAGCTGCCTCTTCCTCCTCCTTGGA
GAAGAGCTATGAACTGCCTGATGGCCAAGTCATCACTATTGGCAATGAGC
GCTTCCGCTGTCCTGAGACACTCTTCCAGCCCTCCTTCATTG

length: 192 bp

EXON	from	to
genomic DNA	3528	3719
mRNA

Primers for Exon 4
4F ttcttgcttcagagcatgactg (22 bp)

4R aagatttccaggaaaatcgtgc (22 bp)

**Primers for Exon 4**
4F	ttcttgcttcagagcatgactg	(22 bp)
4R	aagatttccaggaaaatcgtgc	(22 bp)

(Annealing temp.: 58° C.)

Amplimer for Exon 4

TTCTTGCTTCAGAGCATGACTGTGATACTCTTTATTTCTGTAGCTGAACG
TGAAATTGTCCGTGACATTAAAGAGAAGCTGTGCTATGTCGCCCTGGATT
TTGAGAATGAGATGGCCACAGCTGCCTCTTCCTCCTCCCTGGAGAAGAGC
TATGAACTGCCTGATGGCCAAGTCATCACTATTGGCAATGAGCGCTTCCG
CTGTCCTGAGACACTCTTCCAGCCCTCCTTCATTGGTGAGTTGTAGGGTC
TGGTGCAGAGGCACGATTTTCCCTGGAAATCTT

length: 283 bp

AMPLIMER	from	to
genomic DNA	3485	3767

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ACTC Exon 5

GTATGGAATCTGCTGGCATCCATGAAACAACTTACAATAGCATCATGAAG
TGTGACATTGATATCCGCAAGGACCTGTATGCCAACAATGTCTTATCTGG
AGGCACCACTATGTACCCTGGTATTGCTGATCGTATGCAGAAGGAAATCA
CTGCTCTGGCTCCTAGCACCATGAAGATTAAG

length: 182 bp

EXON	from	to
genomic DNA	4514	4695
mRNA

Primers for Exon 5
5F ttgacctgaatgcactgtgatg (22 bp)

5R tagaataccaagacctgcctcg (22 bp)

**Primers for Exon 5**
5F	ttgacctgaatgcactgtgatg	(22 bp)
5R	tagaataccaagacctgcctcg	(22 bp)

(Annealing temp.: 58° C.)

Amplimer for Exon 5

TTGACCTGAATGCACTGTGATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG
TGTGTGTGTGTGTGTGACTCGTTCCCAGGTATGGAATCTGCTGGCATCCA
TGAAACAACTTACAATAGCATCATGAAGTGTGACATTGATATCCGCAAGG
ACCTGTATGCCAACAATGTCTTATCTGGAGGCACCACTATGTACCCTGGT
ATTGCTGATCGTATGCAGAAGGAAATCACTGCTCTGGCTCCTAGCACCAT
GAAGATTAAGGTAAAGAACTTTTGTGAGTGGGAGATCCGAGGCAAGTCTT
GGTATTCTA

length: 309 bp

AMPLIMER	from	to
genomic DNA	4436	4744

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ACTC Exon 6

ATTATTGCTCCCCCTGAGCGTAAATACTCTGTCTGGATTGGGGGCTCCAT
CCTGGCCTCTCTGTCCACCTTCCAGCAAATGTGGATTAGCAAGCAAGAGT
ACGATGAGGCAGGCCCATCCATTGTCCACCGCAAATGCTTCTAA

length: 144 bp

EXON	from	to
genomic DNA	5254	5397
mRNA

Primers for Exon 6
6F ttggaacttcagagttcactgg (22 bp)

6R aagaagcataataccgtcatcc (22 bp)

**Primers for Exon 6**
6F	ttggaacttcagagttcactgg	(22 bp)
6R	aagaagcataataccgtcatcc	(22 bp)

(Annealing temp.: 58° C.)

Amplimer for Exon 6

TTGGAACTTCAGAGTTCACTGGAAGTTTTTGTTTTCTTTCTGCAGATTAT
TGCTCCCCCTGAGCGTAAATACTCTGTCTGGATTGGGGGCTCCATCCTGG
CCTCTCTGTCCACCTTCCAGCAAATGTGGATTAGCAAGCAAGAGTACGAT
GAGGCAGGCCCATCCATTGTCCACCGCAAATGCTTCTAAGATGCCTTCTC
TCTCCATCTACCTTCCAGTCAGGATGACGGTATTATGCTTCTT

length: 243 bp

AMPLIMER	from	to
genomic DNA	5209	5451

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Last modified: April 24, 2006