Quantitative proteomics seeks to measure the complete protein composition of a cell. Advances in mass spectrometry, and especially the ability to determine peptide sequences by tandem mass spectrometry and protein database searching, have begun to make this goal feasible. Combined with biochemical purification, mass spectrometry also has the ability to provide detailed information about the protein components of macromolecular complexes and subcellular organelles. Recent work from the Church laboratory (Jaffe, et al.) has used mass spectrometry of whole cell extracts to detect over 80% of the predicted proteins in the bacterium M. pneumoniae. We are optimizing this system for use with highly purified organelles to generate catalogs of protein localization and modification states in yeast.

In the future, this section will contain:

• protocols and software
• our results