Protocols and How-To's
My
PCR protocol
for fluorescent markers
How to
pool primer plates
with the Biomek robot
How to
do complex pipetting
(REVEAL plate to sequencing plate) with the Biomek robot
How to
make a plate record
for the ABI 3730 sequencer at the HMS Biopolymers Facility.
How to
calculate two-point lod scores
with mlinker on Salt2 (way outdated).
How to
calculate a multipoint lod score
.
What do all those numbers mean in LINKAGE
pedigree and parameter files
?
Size standard peaks
for ABI genotyping gels.
A brief
Unix primer
How to
BLAST against your own local database
using GCG.
Miscellaneous lab protocols
Nick Sparks's ImageJ cheat sheet
(Microsoft Word document).
Really old stuff
How to
pour
an ABI gel
How to
run
an ABI gel
How to
analyze
a gel using GeneScan
How to
determine genotypes
with Genotyper
Steve DePalma, depalma @ rascal.med.harvard.edu
http://genetics.med.harvard.edu/~depalma