Protocol P.2

Ponceau Staining of Nitrocellulose

This is a quick and easy way to visualize proteins transfered to nitrocellulose following SDS PAGE. It is about half as sensitive as coomasie blue staining.

Solutions

1X Ponceau Stain

0.1% Ponceau-S 0.5 g Ponceau-S (Fisher BP103-50)

1% Acetic Acid 5 ml acetic acid

up to 500 ml Q

store at room temperature

Ponceau Destain

1% acetic acid 5 ml acetic acid

up to 500 ml Q

store at room temperature

Procedure

• Following transfer to nitrocellulose quickly place the membrane in the 1X Ponceau Stain and shake for approximately 5 minutes.

• Transfer the membrane to the Ponceau Destain and shake until the bands are visible.

• The molecular weight markers can be permanently marked using a S&S Pen even when the membrane is still wet.