Bacteria

Displaying 1 - 500 of 2807

Strain Name	Strain Alias	Source Sort descending	Comments	Host	Host Markers	Plasmids	Plasmid Markers

							hml
							%hml%

FB2491	pGN965					pGN965
						pNOY436
				fb2195
						phz1
	pms4
pms4
FB2516



						pMS37
						psh17-4
FB2114
%FB2114%

fb2044
	dh5a
	dh5alpha
fb843
fb843
	dh5a

						pLBS27
						pLBS27
						pMRFW2
fb603
FB2694			Yeast 2009; 26: 185-192			pFA6-6xGLY-FLAG-KanMX	6xGLY-FLAG-KanMX
FB2695			Yeast 2009; 26: 185-192			pFA6-6xGLY-3xFLAG-KanMX	6xGLY-3xFLAG-KanMX
FB2696			Yeast 2009; 26: 185-192			pFA6-6xGLY-Myc-KanMX	6xGLY-Myc-KanMX
fb2697	CKB200		EcoRI-BamHI fragment of pCK198 ligated into BamHI-EcoRI digested pRS306. For two-step integration of GAL10 poly-A+ site mutant.	DH5a			ampr, ColE1 ori, pRS306 backbone (URA3/ARS)
FB

FB
fb
FB2702	pLP891				SIR2 HIS3	pLP891
FB2703	pLP1047				SIR3 HIS3	pLP1047
FB2704	pLP2206				SIR4 HIS3	pLP2206
FB2705	pPK128				HTA1-HTB1 HHT1-HHF1 LEU2	pPK128
FB2706				DH5a	SPO74-GFP	pRS314 SPO74-GFP
FB2707				DH5a	SPO21-GFP	pRS316 SPO21-GFP

FB2706				DH5a		pRS314	SPO74-GFP
FB2707				DH5a		pRS316	SPO21-GFP
FB2727	pCK1		Use pCK4 for biotap-xl preps	DH5a		pCK1	pFA6a + Biotin - 1x protein A
FB2728	pCK3		Use pCK4 for biotap-xl preps			pCK3	pFA6a + Biotin - 2x TEV - 2x protein A
FB2729	pCK4		Use pCK4 for biotap-xl preps			pCK4	pFA6a + 6xGLY - Biotin - 2x TEV - 2x protein A
FB2751	TG11		Stored as plasmid DNA, not in bacteria.	None		pTAL08
FB2752	TG10		Stored as plasmid DNA, not in bacteria.	None		pRS406-CMV-lacI-FLAG	CMV-lacI-3xFLAG
FB2783	pBK257		SATAY method. ADE2 is not expressed until transposon hops, so yeast transformants will be Ura+ Ade-	DH5a	AmpR		CEN ADE2 URA3 TPase(103-807)4x
FB2843	pKW26			DH5alpha	PRS414 + z3evprom-RNH1(wt)-3xV5-adh1term	pKW26
FB2844	pRS416		Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (1/4)			pRS416
FB2845	pRS416		Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (2/4)			pRS416
FB2846	pRS416		Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (3/4)			pRS416
RB2847	pRS416		Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (4/4)			pRS416
FB1775			pAB157 w/o HIII msc. fragment	?.........		pAB203	LEU2 Amp (H3d.H4)d16'
FB1776	pAB204		pAb157 w/o BspI-RI fragment both BspI site and RI site still there	..........		pAB204	LEU2 Amp (H3-H4d)d16'
FB1790	pSR52-1		See book	MH1.......		pSR52-1	MET14
FB1943	pDR1			DH5a......		pDR1	HA-Tagged SPT3 @N-terminus
FB1944	pDR76			DH5a......		pDR76	"2micron, GAL-HA1-SPT3 M188V
FB1945	pDR16			DH5a......		pDR16	"CEN, LEU2, GAL-SPT3+
FB1946	pDR18			DH5a......		pDR18	"CEN, LEU2, GAL-SPT3 M188V
FB1947	pDR20			DH5a......		pDR20	"CEN, LEU2, SPT3 Deletion DR
FB1948	pDR21			DH5a......		pDR21	"2micron, LEU2, SPT3+
FB1949	pDR23			DH5a......		pDR23	"2micron, LEU2, SPT3 M188V
FB1950	pDR25			DH5a......		pDR25	"2micron, LEU2, SPT3-D(DR)
FB1951	pDR32			DH5a......		pDR32	"CEN, LEU2, GAL-HA-SPT3
FB1952	pDR34			DH5a......		pDR34	"CEN, LEU2, NHP6a
FB1953	pDR77			DH5a......		pDR77	"URA3, SPT3-Y193C
FB1954	pDR78			DH5a......		pDR78	"URA3, SPT3-D(DR)
FB1955	pDR79			DH5a......		pDR79	"2micron, URA3, GAL-SPT3D (DR)
FB1956	pDR49			MH1.......		pDR49	"CEN, LEU2, NHP6a
FB1957	pDR73			DH5a......		pDR73	"TRP, NHP6a
FB1958	pDR60			DH5a......		pDR60	"TRP1, NHP6b
FB1959	pDR61			DH5a......		pDR61	"CEN, LEU2, NHP6b
FB1960	pDR63			DH5a......		pDR63	"2micron, LEU2, NHP6b
FB1968	pCDNA18			HB101.....		pCDNA18	"CEN, URA3
FB1969	pCDNA24			HB101.....		pCDNA24	"CEN, URA3, YNL027W
FB1970	pCDNA31			HB101.....		pCDNA31	"CEN, URA3, GAL-YLL022C
FB1971	pCDNA32			HB101.....		pCDNA32	"CEN, URA3, GAL-YDL084W
FB1972	pCDNA66			HB101.....		pCDNA66	"CEN, URA3, GAL-URA5
FB1973	pCDNA69			HB101.....		pCDNA69	"CEN, URA3, GAL-BMH2
FB1974	pCDNA79			HB101.....		pCDNA79	"CEN, URA3, GAL, TYE7
FB1975	pCDNA80			HB101.....		pCDNA80	"CEN, URA3, GAL-URA5
FB1976	pCDNA88			HB101.....		pCDNA88	"CEN, URA3, GAL-cMD1
FB1977	pCDNA94			HB101.....		pCDNA94	"CEN, URA3, DIG1
FB1978	pCDNA103			HB101.....		pCDNA103	"CEN, URA3, RAD23
FB1979	pCDNA50			HB101.....		pCDNA50	"CEN, URA3, RPS12
FB1980	pEMBL-Yex4			DH5a......		pEMBL-Yex4	"URA3, LEU2D, 2micron, CYC-Gal
FB1983	pAD1-23-6			DH5a......		pAD1-23-6	"TRP1, AmpR, his4-912dD44-lacZ(23)
FB1984	pAD1-26-8			DH5a......		pAD1-26-8	"TRP1, AmpR, his4-912dD44-lacZ(26)
FB1989				.L-1 Blue.		HDF1D::TRP1	TRP1 HDF1D
FB32	PNN49			LE392.....	met r- m+ SupE SupF	YRp14
FB33	PNN50			LE392.....	met r- m+ SupE SupF	YRp15
FB38			pAJ50 entered elsewhere	JM101.....	(lac pro)del supE B1/ F' traD36 proAB LacIq lac7M15	pAJ50
FB116	K38			K38.46....	HfrC
FB124			FB124-FB152 all derivatives of pFW32	HB101.....		pFW32-302	spt3-302
FB125				HB101.....		pFW32-306	spt3-306
FB126				HB101.....		pFW32-307	spt3-307
FB127				HB101.....		pFW32-309	spt3-309
FB157	JM103		"Find markers in most catalogs, eg NE Biolabs, BRL; M13 host "	JM103.....		F'pro
FB186			deleted	.B101.....
FB256			out of frame	HB101.M15.		pFW68	amp URA3 lys2-128delta::lacZ
FB258			RI 1.4 kb fragment contains TRP1 and ARS1 into RI site of pBR322	.B101.M15.		YRp7	TRP1 ars1 tet amp
FB260			RI-Xho of SPT3	RR1delM15.		pFW42	amp SPT3
FB281	JM107		streak on M9 glucose + B1 to hold F'	JM107.....	del(lac-pro) thi gyrA96 endA1 hsdR17 relA1 supE44 (F' traD36 proAB lac Iq lacZdelM15)	none
FB1440				.B1.1.....		pT2
FB1441				.B1.1.....		pT1-3B
FB1442				.B1.1.....		pIP5469
FB2053	pSR33			MH1.......		pSR33	GAL1pr-B42-TBP/TRP1/2micron
FB2287	p-GCN5		for making recombinant GCN5 for HAT assays	BL21(DE3).		pRSetB-GCN5	GCN5
xit
						pGH127
						pGH%
FB
						pFW217
						pRM200
						pRM%
							ura3 his3
							snf1
							ura3
							%FLAG%
FB439			DO NOT USE	.B101.....
FB440			DO NOT USE	.B101.....
FB485				.B101.....
FB579			"H3-SphI insert, around 6 kb "	..........		pFW202	pCGS42 RSP5
FB626	JM109		see notebook	JM109.....	recA1 endA1 gyr96 thi hsdR17(rk- mk+) supE44 relA1 lambda- del(lac pro AB)/ F' traD36 proAB lacIqZd	none
FB628			see notebook	HB101.....		pSS31	S. pombe ade6+ in pDB248
FB629			see notebook	HB101.....		pYK311	S. pombe leu1+ pBR322
FB774				.B101.....		pGC21
FB782				.B101.....
FB128		.		DB6507....		pFW32-318	spt3-318
FB129		.		DB6507....		pFW32-319	spt3-319
FB130		.		DB6507....		pFW32-322	spt3-322
FB253		.	"EMBO 2, 1971-1794 (1983); for lacZ fusions for antibodies "	RR1delM15.	lacZdel r-	pUR290	ampR lacZ
FB600	MC1065	"Casadaban, Struhl, Raymond	"For scoring trp, use drop out media. Also see FB326. "	MC1065*...	"X1486 M+h12 leuB600 trpC9830 pyrF::Tn5 hisB463 dellacX74 strA galU,K	none
FB482		"D. Norris, M.A. Osley	autonomous due to ars near trt1	HB101.....		pdeltrt1-URA3	del(hta1-htb1::URA3) in pUC18
FB484		"D.Norris, M.A. Osley		HB101.....		YEp24-TRT1	YEp24 HTA1-HTB1
FB586		"Daria Hekmatpanah, Fink	1.1 kb insert	HB101.....		pDH22	pUC18 HIS4 SalI-PvuII into SalI-SmaI
FB587		"Daria Hekmatpenah, Fink	1.45 kb insert derived from B701	HB101.....	pDH23	pDH23	pCU18 his4-912delta SalI-PvuII into SalI-SmaI
FB588		"Daria Hekmatpenah, Fink	1.65 kb insert from pRB153 (Botstein)	HB101.....		pDH25	pUC18 ACT1 BamHI-HindIII into same
FB254		"EMBO 2, 1791-1794 (1983)	for lacZ fusions for antibodies	RR1delM15.	lacZdel r-	pUR291	lacZ amp
FB255		"EMBO 2, 1971-1794 (1983)	for lacZ fusions for antibodies	RR1delM15.	lacZdel r-	pUR292	amp lacZ
FB118	B155	"Fink, Phil Carter	orientation of insert unknown	?.101.....		B155	Ty912 HIS4 URA3 amp
FB272		"Fred, Christy, Patty	spt3-271(UAA)	HB101.....		pFW79	amp URA3 spt3-271(UAA)
FB270		"Fred, Patty, and Christy	spt3-244 is an ochre mutation	HB101.....		pFW77	amp URA3 spt3-244
FB271		"Fred, Patty, and Christy	spt3-269(UGA)	HB101.....		pFW78	amp URA3 spt3-269(UGA)
FB311	B661	"J. Bact. 156, 625	"pBR325 derivative, leu2-d for high copy number, in G.R. Fink collection "	unknown...		pMP78-1	leu2-d amp 2 micron camR
FB238		"J. Gorman, SmithKline	for promoter fusions to galK	E. coli...		pYSK7-54	amp TRP1 'galK CEN3
FB239		"J. Gorman, SmithKline	for protein fusions to galK	.. coli...		pYSK9-8	amp TRP1 galK CEN3
FB240		"J. Gorman, SmithKline	for protein fusions to galK	.. coli...		pYSK9-10	amp TRP1 'galK CEN3
FB241		"J. Gorman, SmithKline	for protein fusions to galK	.. coli...		pYSK9-12	amp TRP1 'galK CEN3
FB914		"K.Ferguson.n, Wigler lab	see notebook	HB101		pAD5	Lerner Epitope
FB2241	YCP50-TRT2	"M.A, OSLEY		DH5A......		YCp50-TRT2	"URA3,CEN,TRT2
FB227		"MCB 4, 657-665, Maynard Olsen	UGA suppressor	..........		pPM600	SUP4-UGA CEN3 ampR TRP1 ARS1
FB226	pPM702	"MCB 4, 657-665; Maynard Olsen	contains an ochre suppressor	..........		pPM702	SUP4-o CEN3 amp TRP1 ARS1
FB859		"Parent, Kramer	PHO5 probe (?) see notebook	HB101.....		p20L	amp PHO5 pBR322
FB367	B987	"PNAS 82, 8557	"small deletion, Nagawa and Fink "	.B101.....		x-99	HIS4-lacZ cen ura ars1 Xho linker
FB368	B994	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-407	HIS4-lacZ amp cen4 ars1 ura3 Xho linker
FB369	B1005	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-101	HIS4-lacZ cen4 ars1 ura3 Xho linker
FB370	B1006	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-122	HIS4-lacZ cen4 ars1 ura3 Xho linker
FB371	B1012	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-7	HIS4-lacZ cen4 ura3 ars1 Xho linker
FB372	B1015	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-198	HIS4-lacZ cen4 ura3 ars1 Xho linker
FB373	B1019	"PNAS 82, 8557	Nagawa and Fink	.B101.....		x-52	HIS4-lacZ cen4 ars1 ura3 Xho linker
FB273		? maybe Ben Hall lab	HindIII fragment of PYK1 in pBR322	HB101.....		pFR2	PYK1 amp
FB2219	pAD19	A Dudley	see notebook for specific mutation	MH1.......		pAD19	"Ty912D44-lacZ (#19), TRP1
FB2221	pAD22	A Dudley	see notebook for specific mutation	MH1.......		pAD22	"TRP1, Ty912D44-lacZ(#22)
FB2222	pAD23	A Dudley	see notebook for specific mutation	MH1.......		pAD23	"TRP1, Ty912D44-lacZ(#23A)
FB2223	pAD24	A Dudley	see notebook for specific mutation	MH1.......		pAD24	"TRP1, Ty912D44-lacZ(#24)
FB2224	pAD27	A Dudley	see notebook for specific mutation	MH1.......		pAD27	"TRP1, Ty912D44-lacZ(clean #18)
FB2225	pAD28	A Dudley	see notebook for specific mutation	MH1.......		pAD28	"TRP1, Ty912D44-lacZ(#12/13)
FB2226	pAD29	A Dudley	see notebook for specific mutation	MH1.......		pAD29	"TRP1, Ty912D44-lacZ(#17/18)
FB2227	pAD30	A Dudley	see notebook for specific mutation	MH1.......		pAD30	"TRP1, Ty912D44-lacZ(#13/18)
FB2228	pAD31	A Dudley	see notebook for specific mutation	MH1.......		pAD31	"TRP1, Ty912D44-lacZ(#23B)
FB2229	pAD25	A Dudley	see notebook for specific mutation	MH1.......		pAD25	"URA3, Ty912D44-lacZ(#25)
FB2435	pGCYC1-402	A. Aguilera		DH5a......		pGCYC1-402	pGAL1-double G-less cassette URA3 CEN
FB2436	p416GAL1	A. Aguilera		DH5a......		p416-GAL1-lacZ	pGAL1-lacZ URA3 CEN
FB2437	pSch227	A. Aguilera		DH5a......		Sch227	"pGAL1-LYS2, URA3 CEN
FB2438	pSch247	A. Aguilera		DH5a......		pSch247	pGAL1-YAT1 URA3 CEN
FB2439	pSch202	A. Aguilera		DH5a......		pSch202	pGAL1-PHO5 URA3 CEN
FB2440	pCM184-LAu	A. Aguilera		DH5a......		pCM184-LAUR	pTet-lacZ-URA3
FB2441	pSch204	A. Aguilera		DH5a......		pSch204	leu2-lacZ-leu2 TRP1 CEN
FB1168	pAB56	A. Bortvin	BglII spt6-140 frag cloned into BamHI site of YCp50 that has BglII-SmaI frag (CEN ARS) deleted. see also FB1167.	HB101.....		pAB56	spt6-140 URA3 ampR
FB1169	pAB1	A. Bortvin	3.5kb SPT5 frag from pMS4 cloned into SmaI site of pJK1521. LexA-SPT5 fusio n= 12-1064 a.a.	HB101.....	sick	pAB1	SPT5-lexA TRP1 CEN ARS ampR
FB1170	pAB57	A. Bortvin	same as FB1169 but fusion of lexA with SPT5 starting at a.a.#632-1064.	HB101.....	sick	pAB57	SPT5-lexA TRP1 CEN ARS ampR
FB1487	pAD1	A. Dudley	1.5kb SacI-ClaI frag from pLG39 (FB1350) containing his4-912dD44-lacZ subclo ned into 4.2kb SacI/ClaI of pRS304	HB101.3...		pAD1	TRP1 ampR his4-912dD44-lacZ
FB1488	pAD11	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid sucloned into PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific mutati on seq.	HB101.3...		pAD11	his4-912dD44-lacZ-pAD11 TRP1 ampR
FB1489	pAD12	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq.	HB101.3...		pAD12	his4-192dD44-lacZ-pAD12 TRP1 ampR
FB1490	pAD13	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487). See enclosed sheets for specific mut ant seq.	HB101.3...		pAD13	his4-912dD44-lacZ-pAD13 TRP1 ampR
FB1491	pAD14	A. Dudley	PacI-BseRI fragment (320bp) from site directed mutagenesis plasmid subcloned into PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific m utant seq.	HB101.3...		pAD14	his4-912dD44-lacZ-pAD14 TRP1 ampR
FB1492	pAD15	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific mutan t seq.	HB101.3...		pAD15	his4-912dD44-lacZ-pAD15 TRP1 ampR
FB1493	pAD16	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq.	HB101.3...		pAD16	his4-912dD44-lacZ-pAD16 TRP1 ampR
FB1494	pAD17	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq.	HB101.3...		pAD17	his4-912dD44-lacZ-pAD17 TRP1 ampR
FB1495	pAD18	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq.	HB101.3...		pAD18	his4-912dD44-lacZ-pAD18 TRP1 ampR
FB1496	pAD21	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq.	HB101.3...		pAD21	his4-912dD44-lacZ-pAD21 TRP1 ampR
FB1594	pAD26	A. Dudley	See enclosed sheets for more info.	HB101.....		pAD26	his4-912dd44-lacZ TRP1 ampR
FB2220	pAD20	A. Dudley	see notebook for specific mutation	MH1.......		pAD20	"TRP1, Ty912D44-lacZ(#20)
FB1140		A. Rowley + Curt Wittenburg		HB101.....
FB2250	pGEMT-HIS1	Aaron Mitchell	for making deletions by PCR	DH5a......		pGEMT-HIS1	C.albicans HIS1
FB2251	pGEMT-URA3	Aaron Mitchell	for making deletions by PCR	DH5a......		pGEMT-URA3	C.albicans URA3
FB2252	pRS-ARG4	Aaron Mitchell	for making deletions by PCR	DH5a......		pRS-ARG4 DSpeI	C.albicans ARG4
FB786		Aaron Mitchell	see FB674 for other IME1; do not give out; see notebook	..........		pHS103	YEp24 IME1
FB2362	pAFS59	Aaron Straight	ref Current Biology 6:1599-1608 1996	Stable2...		pAFS59	"lac operator repeat, YIPlac128-LEU2
FB2363	pAFS135	Aaron Straight	ref Current Biology 6:1599-1608 1996	Stable2...		pAFS135	"HIS3p-GFP12-LacI-I12, pRS303
FB2364	pAFS144	Aaron Straight	ref Current Biology 6:1599-1608 1996	Stable2...		pAFS144	HIS3p-GFP13-LacI-I12
FB2365	pAFS91	Aaron Straight	ref Current Biology 6:1599-1608 1996	DH5a.e2...		pAFS91	HIS3p-GFP(s65t)-TUB1
FB2366	pAFS52	Aaron Straight	ref Current Biology 6:1599-1608 1996	Stable2...		pAFS52	lacO YIPlac204
FB2367	pAFS114	Aaron Straight	ref Current Biology 6:1599-1608 1996	DH5a.e2...		pAFS114	TEL4 lacO LEU2
FB2791		Aguilera Lab	contains gal inducible RNasH1; from Herrera-Moyano...Aguilera, 2014			pRS416-gal-rnh1	pRS416-gal-rnh1 trp, amp
FB2790		Aguilera Lab	contains gal inducible AID; from Garcia-Pichardo...Aguilera et al, 2017, Mol Cel			pLZAID	pSCH204-gal-aid ura, amp
FB2561	pHM27	Alain Verreault	YcP22			pHM27	HHT1-HHF1 CEN TRP1
FB2562	pHM146	Alain Verreault	YCp22			pHM146	hht1K56A-HHF1 CEN TRP1
FB2563	pHM147	Alain Verreault	YCp22			pHM147	hht1K56Q-HHF1 CEN TRP1
FB2564	pHM148	Alain Verreault	YCp22			pHM148	hht1K56R-HHF1 CEN TRP1
FB2566	pHM164	Alain Verreault	YIplac204 TRP1 cut with Bsu36I to integrate, but doesn't work with trp1D63			pHM164	hht1K56A-HHF1 TRP1
FB2567	pHM165	Alain Verreault	YIplac204 TRP1 cut with Bsu36I to integrate but doesn't work for trp1d63			pHM165	hht1K56Q-HHF1 TRP1
FB2568	pHM166	Alain Verreault	YIplac204 TRP1 cut with Bsu36I to integrate but doesn't work for trp1D63			pHM166	hht1K56R-HHF1 TRP1
FB2340	p25	Alan Goldstein	"nourseothricin resistance, Yeast 15, 1541-1553 (1999) "	DH5a......		p25	"natMX4, pFA6 vector backbone
FB2341	p29	Alan Goldstein	"Bialaphos resistance, Yeast 15, 1541-1553 (1999) "	DH5a......		p29	"patMX4, pFA6 vector backbone
FB2342	p32	Alan Goldstein	"Hygromycin B resistance, Yeast 15, 1541-1553 (1999) "	DH5a......		p32	"hphMX4, pFA6 vector backbone
FB2383	pAS249	Alan Sachs	YCp50	DH5a......		pAS249	URA3 CEN PAN1
FB672		Alani and Kleckner	"Genetics 117, 5-12 "	HB101.....		pNKY54	HIS4-URA3 TRP1
FB1774		Alex	A derivative of pAB148 looking ARS CEN sequences integrate via BstEII in LEU 2	unknown...		pAB157	LEU2 Amp
FB1826	pAB71	Alex Bortvin		HB101.....		pAB71	"CEN, ARS1, LEU2, DED1::GCN4
FB2275	pUC13D16'	Alex Bortvin		DH5a......		pUC13D16'	URA3 with flanking BamH1 sites
FB1777	pAB221	Alex.	pAB157 w/o aa 4-30 in H3	.nknown...		pAB221	LEU2 Amp (H3d4.30-H4)
FB1778	pAB224	Alex.	pAB157 w/PCR fragment from FY869 carrying bur5-1 mutation	.nknown...		pAB224	LEU2 Amp.
FB2786	ASB1014	Ameet Shetty	made by swapping NatMx cassette in FB2530 to hphMX6 from FB2543 using EcoRV and BglII	DH5a		pFA6a-hphMX6-P3nmt1	P3nmt1 hphMX6
FB2787	ASB1016	Ameet Shetty	made by swapping NatMx cassette in FB2531 to hphMX6 from FB2543 using EcoRV and BglII	DH5a		pFA6a-hphMX6-P41nmt1	P41nmt1 hphMX6
FB2788	ASB1018	Ameet Shetty	made by swapping NatMx cassette in FB2532 to hphMX6 from FB2543 using EcoRV and BglII	DH5a		pFA6a-hphMX6-P81nmt1	P81nmt1 hphMX6
FB2515	p1810-NAT	Amine		DH5a		p1810-NAT	replace kanR with NatR
FB2446	pLL65	Amine	Lefebvre and Smith 1993	DH5a......		pLL65	LEU2 SPT2-359 2micron
FB2447	pLL10	Amine	Lefebvre and Smith 1993	DH5a......		pLL10	LEU2 2micron SPT2
FB2448	pLL18	Amine	Lefebvre and Smith 1993	DH5a......		pLL18	LEU2 2micron SPT2-1
FB2456	pET-15b	Amine		DH5a		pET-15b
FB2420	pAAD3	Andrea Duina		DH5a......		pAAD3	"hht2-3-HHF2 TRP1, CEN
FB2421	pAAD5	Andrea Duina		DH5a......		pAAD5	hht2-5-HHF2 TRP1 CEN
FB2422	pAAD7	Andrea Duina		DH5a......		pAAD7	hht2-7-HHF2 TRP1 CEN
FB2423	pAAD11	Andrea Duina		DH5a......		pAAD11	hht2-11-HHF2 TRP1 CEN
FB2424	pAAD41	Andrea Duina		DH5a......		pAAD41	hht2-41-HHF2 TRP1 CEN
FB2425	pAAD44	Andrea Duina		DH5a......		pAAD44	hht2-44-HHF2 TRP1 CEN
FB2426	pAAD48	Andrea Duina		DH5a......		pAAD48	hht2-48-HHF2 TRP1 CEN
FB2427	pAAD49	Andrea Duina		DH5a......		pAAD49	hht2-48-HHF2 TRP1 CEN
FB2428	pAAD51	Andrea Duina		DH5a......		pAAD51	hht2-51-HHF2 TRP1 CEN
FB2429	pAAD56	Andrea Duina		DH5a		pAAD56	hht2-56-HHF2 TRP1 CEN
FB2430	pAAD57	Andrea Duina		DH5a......		pAAD57	hht2-57-HHF2 TRP1 CEN
FB2431	pAAD180	Andrea Duina		MH1.......		pAAD180	hht2-11-HHF2 TRP1 2micron
FB1990	pKH149	Andrew Murray's lab		HB101.....		pKH149	mad1D::HIS3
FB1991	pKH160	Andrew Murray's lab		HB101.....		pKH160	mad1D::URA3
FB2576	p433	Angelica Amon	AF mutation introduces a BsmI site in cdc28			p433	cdc28-T18A Y19F URA3
FB860		Anne Chiang	SPT9 candidate E from YCp50 library	HB101.....		pAC1	SPT9 URA3 amp
FB861		Anne Chiang	no SPT9 function; see notebook	TB1.1.....		pAC10	"SPT9 subclone, Bam deletion
FB862		Anne Chiang	probably not functional	TB1.1.....		pAC12	pRS306 SPT9 Bam-Sal
FB863		Anne Chiang	partial SPT9 function	TB1.1.....		pAC14	SPT9 Mlu deletion clone in YCp50
FB864		Anne Chiang	no SPT9 function	.B1.1.....		pAC16	YCp50 SPT9 HindIII deletion clone
FB865		Anne Chiang	no SPT9 function	.B1.1.....		pAC18	YCp50 SPT9 BglII clone
FB866		Anne Chiang	probably non-functional; should be rechecked	TB1.1.....		pAC20	pRS316 SPT9 region Bam fragment
FB867		Anne Chiang	not tested for function	.B1.1.....		pAC22	"SPT9, SacI piece reclosed
FB2269	pYSL12	Anne Dranginis	MCB V9 1/98 161-171	DH5a......		pYSL12	"pRS425, LEU2, 2micron, FLO11
FB2270	pYSL13	Anne Dranginis	MCB V9 1/98 161-171	DH5a......		pYSL13	"pRS426, URA3, 2micron, FLO11
FB511		Anne Happel	"H3-Bam fragment, no activity "	HB101.....		pRSP4-27	rsp4-27 pBR322
FB652		Anne Happel	for spt3::TRP1 null	HB101.....		pAH100	spt3-203::TRP1 pCGS42
FB2560	pET28bSPT3	Annie K.	Kan resistant	DH5a		pET28b-SPT3	SPT3
FB632		Antebe and Fink	"J. Bact. 166, 313 (1986); subclone of pTLG2 into YIp5; RI-H3 fragment "	HB101.....		pAA1	YIp5 GAL2
FB2714	JF340116	Anthony Carr	Watson et al. 2011, Gene 484 pp 75-85 Derived from pAW8 from Watson et al. 2008, Gene 407, pp 63-74	DH5a	AmpR	pAW8 ENdeI-cyEGFP
FB2715	JF340118	Anthony Carr	Watson et al. 2011, Gene 484 pp 75-85 Derived from pAW8 from Watson et al. 2008, Gene 407, pp 63-74	DH5a	AmpR	pAW8 ENdeI-C3HA
FB2716	JF340115	Anthony Carr	Watson et al. 2011, Gene 484 pp 75-85 Derived from pAW8 from Watson et al. 2008, Gene 407, pp 63-74	DH5a	AmpR	pAW8 ENdeI
FB2708	pM40-3	Apolife / Nalini Motwani		DH5a		pM40-3	HPAIx, AmpR
FB2709	PMC50-2	Apolife / Nalini Motwani		DH5a		PMC50-2	AmpR
FB2708		ApoLife, Nalini Motwani	Do not give out.	DH5a			pM40-3 HPAIX AmpR
FB2709		ApoLife, Nalini Motwani	Do not give out	DH5a			pMC50-2 AmpR
FB2717		Arndt Lab	Derived from pWZ414-F12 (Zhang et al EMBO J. 1998 June1; 17(11):3155-3167. From Shilatifard Histone Mutant Library. Obtained from Arndt Lab.	DH5a		hht2-K36A-HHF2	hht2-K36A-HHF2 TRP1 CEN3
FB2085	pSZH	Arno Greenleaf	Ref. MCB 15(10) 5716 (1995)	KC8.......		pSZH	ctk1D::HIS3
FB2180	pJYC1513	Arno Greenleaf		unknown...		pJYC1513	"CTK1, pRS316
FB1444	pHIBBB90	ATCC (#84835)	human SPT4 HindIII-NotI clone	XL1-Blue..		pHIBBB90	hSPT4 ampR
FB1811		Audy Hoyt	bub2::URA3	?.........		pTR24	ClaI SacI
FB2203	P102	B Cairns		DH5a......		p102	URA3 CEN SWP59
FB2205	pS340	B Cairns	NPL6 aka RSC7	DH5a......		pS340	Ycp50 URA3 CEN NPL6
FB2206	p138	b cairns		dh5a......		p138	"URA3,CEN,RSC6
FB2207	p147	b cairns		DH5a......		p147	"URA3,CEN,TFG3
FB2208	p149	b cairns		DH5a......		p149	URA3 CEN SWP73
FB2209	p151	b cairns	TAF130 and RSC14 separated by only 80bp	DH5a......		p151	"URA3,2micron,RSC14 w/TAF130
FB2210	p164	b cairns		DH5a......		p164	"URA3,CEN,RSC4
FB2211	p166	b cairns		DH5a......		p166	"URA3,CEN,RSC1
FB2212	p178	b cairns		DH5a......		p178	URA3 CEN RSC3
FB2213	p197	b cairns		DH5a......		p197	TRP1 CEN RSC1
FB2204	pBL50	B Laurent via B Cairns		DH5a......		pBL50	URA3 2micron STH1
FB2216	PHJM1	B OLSEN		?.5a......		PHJM1	"pBluescript, 5' end of SUPT3H cDNA
FB2217	p123089	B Olsen	pBluescript derivative	?.5a......		p123089	Est clone p123089 into pT7T30
FB2723	pAJ044	B. Atkins via JJ Heinisch	Jendretzki et al, Mol Genet. Genomics 2009	DH5a		pAJ044	IQG1 URA3 AmpR 2u
FB1260	RZ1032	B. Berg	for dut ung mutagenesis	RZ1032....	dut1 ung1 thi1 relA HfrKL16 PO/45 [lysA61-62] 2bd-279::Th10 supE44
FB1290	pL909	B. Berg (U. Chicago)	REF: Apricio et al. CELL 66: 1279-1287 (1991) contains a 3.6kb ADE2 BamHI frag in YEp13.	unknown...		pL909	ADE2 ampR
FB1291	pdADE2	B. Berg (U. Chicago)	REF: Aparicio et al. CELL 66:1279-1287(1991) Alani et al. Genetics 116:541 (1987) =ADE2::hisG URA3 disruption in bluescript derivative pV21	unknown...		pdADE2	ADE2::hisGURA3 ampR
FB1858	GHB113	B. Cairns	"Leu2 inserted in NdeI-PstI, removes most of exon II, use NotI/XhoI to libera te fragment for 1 step integrate "	MH1.......		tfg3d::LEU2	tfg3d::LEU2
FB735		B. Daignan-Fornier	This strain is for making MuLEU2 hops; see notebook	JM109.....	JM109 MuCts ZZ4 CAMR
FB736		B. Daignon-Fornier	see notebook	M8820.....
FB77		B. Errede		HB101.....		pAB25	CYC7
FB1377	pBBB2	Barbara Berg	=NdeI-BamHI frag containing SPT3 from pDE118-8 into NdeI-BamHI of pET-3C (FB 894) SEE SHEETS IN NOTEBOOK.	BL21(DE3).	see enclosed sheets for info on host. Bl21(DE3) also contains pLysS w hich is CmR.	pBBB2	T7 promoter ampR SPT3 CmR
FB1378	pBBB9	Barbara Berg	PvuII-SalI frag containing rsp5 from pFW201 subcloned into pRS316	VJS533.3).	#NAME?	pBBB9	ampR CEN ARS URA3 rsp5 PvuII-SalI frag
FB1379	pBBB10	Barbara Berg	BamHI-HindIII frag containing rsp5 from pFW201 subcloned into pRS316	VJS533.3).	#NAME?	pBBB10	ampR CEN ARS URA3 rsp5 BamHI-HindIII frag
FB1380	pBBB11	Barbara Berg	BamHI-BamHI frag containing rsp5 from pFW201 subcloned into pRS316	VJS533.3).	#NAME?	pBBB11	ampR CEN ARS URA3 rsp5 BamHI-BamHI frag
FB1381	pBBB12	Barbara Berg	SalI-HindIII frag containing rsp5 from pFW201 subcloned into pRS316. *NOT E: construct may be incorrect*	VJS533.3).	#NAME?	pBBB12	ampR CEN ARS URA3 rsp5 SalI-HindIII frag
FB1382	pBBB13	Barbara Berg	KpnI-HindIII frag containing rsp5 from pFW201 subcloned into pRS316. *NOT E: constrcuct may be incorrect!*	VJS533.3).	#NAME?	pBBB13	ampR CEN ARS URA3 rsp5 KpnI-HindIII frag
FB1383	pBBB14	Barbara Berg	BamHI-SalI frag containing rsp5 from pFW201 subcloned into pRS316	VJS533.3).	#NAME?	pBBB14	ampR CEN ARS URA3 rsp5 BamHI-SalI frag
FB2202	p101	BCairns		DH5a......		p101	"URA3,CEN,SWP61
FB2817	RGBO5	Beate Schwer					TRP1 CEN ampR CEG1
FB2818	RGBO6	Beate Schwer					TRP1 CEN ampR ceg1-3
FB2819	RGBO7	Beate Schwer					TRP1 CEN ampR ceg1-13
FB2820	RGBO8	Beate Schwer					TRP1 CEN ampR ABD1
FB2821	RGBO9	Beate Schwer					TRP1 CEN ampR abd1-5
FB2822	RGBO10	Beate Schwer					TRP1 CEN ampR abd1-8
FB2182		Beckwith Lab		DHB4......
FB2302	pBAD18	Beckwith lab	for expressing protein in bacteria see notebook	DH5a.rean.		pBAD18	"pBR322 Ori, Ara-inducible
FB2303	pBAD24	Beckwith Lab	for expressing protein in bacteria see notebook	DH5a.rean.		pBAD24	pBR322 based ori Ara-inducible
FB2304	pBAD33	Beckwith Lab	for expressing protein in bacteria see notebook	DH5a.rean.	CM res	pBAD33	"pACYC origin, Ara-inducible
FB2664	pAG25-TAP	Bell lab	Precision protease in place of TEV	DH5a		pAG25-TAP-PP	natMX6 TAP tag Precision protease
FB2082		Berger Lab		DH5a......		A/C GV	"gal4-VP16 Leu2, CEN
FB2083		Berger Lab	F to A mutant	DH5a......		A/C FA	gal4-VP16 LEU2 CEN
FB2084		Berger Lab		DH5a......		G/V	"2micron Gal4-VP16, LEU2
FB2001	pRS426	Berger lab		DH5a......		pRS426	"gal1-ADA2, URA3, 2micron
FB2002	pRS425	Berger Lab	JBC 271:9(1996)	DH5a......		pRS425	"gal1-GCN5, LEU2, 2 micron, ampR
FB820		Betsy	Alias is hcs1-101::LEU2; see notebook	HB101.....		pBM10	spt16-101::LEU2
FB821		Betsy		TB1.1.....		pBM13	pRS314 SPT16 Bam
FB822		Betsy	y	TB1.1.....		pBM15	pRS315 SPT16 Bam
FB823		Betsy		HB101.....		pBM29	YCp50 SPT4 RI-H3
FB824		Betsy	Cen plasmid; bacterial strain is very sick	HB101.....		pBM24	pSM453 SPT5 Xho-EagI
FB825		Betsy	see notebook for making null instructions	TB1.1.....		pBM18	spt5-del201::LEU2
FB826		Betsy	for making spt5 null; see notebook	TB1.1.....		pBM19	spt5-del202::LEU2
FB1098	BBM11	Betsy Malone	Sal-Xho of LEU2 in YEp13 inserted into Sal site of pBM8	HB101.....		pBM11	spt16-102::LEU2 AmpR URA3
FB1099	BBM22	Betsy Malone	Bam frag of spt16-102::LEU2 from pBM11 (FB1098) into Bam site of YEp24	HB101.....		pBM22	spt16-102::LEU2 AmpR URA3 TetS 2micron ori
FB1100	BBM23	Betsy Malone	H3-Sal frag of SPT16 (from pBM7) put into pBR322.**This frag is probably N ONfunctional---it was made as a NORTHERN PROBE**	HB101.....		pBM23	SPT16 frag AmpR TetS ori
FB1101	BBM25	Betsy Malone	ScaI-H3 frag of SPT4 into H3-NruI site of YCp50	HB101.....		pBM25	SPT4 AmpR URA3 CEN ARS
FB1102	BBM26	Betsy Malone	RI-PvuII of SPT4 into RI-SmaI of YEp355 ***makes an in frame SPT4-lacZ fusio n	HB101.....		pBM26	SPT4-lacZ AmpR URA3 2micron
FB1103	BBM27	Betsy Malone	RI-H3 frag of SPT4 into RI-H3 of pUC19	HB101.....		pBM27	SPT4 AmpR ori
FB1104	BBM33	Betsy Malone	spt16-23 Bam frag into pRS314 (= a derivative of pBM13) Yeast strain=L722 a lias BM101	HB101.....		pBM33	spt16-23 AmpR TRP1 CEN ARS ori
FB1105	BBM34	Betsy Malone	spt16-35 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L723 a lias BM102	HB101.....		pBM34	spt16-35 AmpR TRP1 CEN ARS ori
FB1106	BBM35	Betsy Malone	spt16-99 Bam Frag into pRS314 (=a derivative of pBM13) Yeast strain= L724 a lias BM103	HB101.....		pBM35	spt16-99 AmpR TRP1 CEN ARS ori
FB1107	BBM37	Betsy Malone	spt16-133 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L725 alias BM124	HB101.....		pBM37	spt16-133 AmpR TRP1 CEN ARS ori
FB1108	BBM39	Betsy Malone	spt16-146 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L726 alias BM126	HB101.....		pBM39	spt16-146 AmpR TRP1 CEN ARS ori
FB1109	BBM42	Betsy Malone	spt16-175 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L727 alias BM129	HB101.....		pBM42	spt16-175 AmpR TRP1 CEN ARS ori
FB1110	BBM44	Betsy Malone	spt16-181 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L728 alias BM131	HB101.....		pBM44	spt16-181 AmpR TRP1 CEN ARS ori
FB1111	BBM45	Betsy Malone	spt16-185 Bam frag in pRS314 (= a derivative of pBM13) Yeast strain= L729 alias BM132	HB101.....		pBM45	spt16-185 AmpR TRP1 CEN ARS ori
FB1112	BBM46	Betsy Malone	spt16-197 Bam frag in pRS314 (= a derivative of pBM13) Yeast strain= L730 a lias BM133	HB101.....		pBM46	spt16-197 AmpR TRP1 CEN ARS ori
FB1113	BBM47	Betsy Malone	SPT4 with Amino acid #7 changed fron Cys to Ser. (in vitro mutagen. of M13 clone 106 with primer BM32. H3 frag subcloned into YCp50)	HB101.....		pBM47	spt4 (Cys7 to Ser) URA3 AmpR CEN ARS
FB1114	BBM49	Betsy Malone	SPT4 with amino acid #7 changed from Cys to His---Has opp orientation as tha t of pBM48. (From in vitro mut. fo M13 clone 106 with primerBM33. H3 fra g subcloned into YCp50.)	HB101.....		pBM49	spt4 (Cys7 to His) URA3 CEN ARS AmpR
FB1115	BBM50	Betsy Malone	SPT4 with amino acid #27 changed from Cys to Ser. (from in vitro mut. of M1 3 clone 106 with primer BM34. H3 frag subcloned into YCp50.	HB101.....		pBM50	spt4 (Cys27 to Ser) AmpR URA3 CEN ARS
FB1116	BBM51	Betsy Malone	from gap repair of pJF42 in L331	HB101.....		pBM51	spt4-289 AmpR URA3 CEN ARS
FB1117	BBM52	Betsy Malone	from gap repair of pJF42 in L351	HB101.....		pBM52	spt4-3 AmpR URA3 CEN ARS
FB1118	BBM53	Betsy Malone	from gap repair of pJF42 in L374	HB101.....		pBM53	spt4-6 AmpR URA3 CEN ARS
FB1119	BBM56	Betsy Malone	NOTE: lerner fusion to the N terminus of SPT4. (from oligo dir. mut. of M13 clone 106 with BM28; H3 frag subcloned into YCp50.)	HB101.....		pBM56	lerner::SPT4 AmpR URA3 CEN ARS
FB1120	BBM57	Betsy Malone	NOTE: lerner fusion to C terminus of SPT4 (from oligo dir mut. of M13 clone 106 with BM29; H3 frag was subcloned into YCp50.	HB101.....		pBM57	SPT4::lerner AmpR URA3 CEN ARS
FB1121	BBM61	Betsy Malone	SPT4 with amino acid #27 changed from Cys to His. (from oligo dir mut. of M1 3 clone 106 with BM35; H3 frag subcloned into YCp50)	HB101.....		pBM61	spt4 (Cys27 to His) AmpR URA3 CEN ARS
FB1122	BBM65	Betsy Malone	NOTE: lerner fusion to the N terminus of SPT4 (H3 frag from mut7C cloned in to H3 site of pCGS42)	HB101 ....		pBM65	lerner::SPT4 AmpR ori URA3 2micron
FB1123	BBM66	Betsy Malone	NOTE: lerner fusion to the C terminus of SPT4 (H3 frag from mut88 cloned int o H3 site of pCGS42.)	HB101.....		pBM66	SPT4::lerner AmpR URA3 2micron ori
FB1124	BBM68	Betsy Malone	insert SPT5 (H3 to KpnI) into YIp356R (Has partial SPT5 function; blue in coli and yeast) E. coli strain is SICK.	TB1.1.....		pBM68	SPT5-lacZ (H3 to KpnI) AmpR URA3
FB1125	BBM72	Betsy Malone	NOTE: lerner fusion to the N terminus of SPT4 (H3 frag from FB1122 [pBM65] cloned into H3 sites of YEp13.)	HB101.....		pBM72	SPT4::lerner AmpR LEU2 2micron TetR
FB1126	BBM76	Betsy Malone	NOTE: =Orientation II. (H3 frag from mut13C into pCGS42)	HB101.....		pBM76	HAI::spt4 (Cys7 to Ser) URA3 2micron AmpR
FB1127	BBM77	Betsy Malone	NOTE: = Orientation I. (H3 frag from mut14C into pCGS42.)	HB101.....		pBM77	HAI::spt4 (Cys7 to His) URA3 2micron AmpR
FB1128	BBM79	Betsy Malone	NOTE: = Orientation I (H3 frag from mut15A cloned into pCGS42.)	HB101.....		pBM79	HAI::spt4 (Cys27 to Ser) URA3 2micron AmpR
FB1129	BBM80	Betsy Malone	NOTE: = Orientaion I (H3 frag from mut16B into pCGS42.)	HB101.....		pBM80	HAI::spt4 (Cys27 to His) URA3 AmpR 2micron
FB1130	BBM83	Betsy Malone	NOTE: = Orientation II (H3 frag from mut18B into pCGS42.)	HB101.....		pBM83	HAI::spt4 (Cys24 to His) URA3 AmpR 2micron
FB1131	BBM84	Betsy Malone	NOTE: =Orientation II (H3 frag from mut19B into pCGS42.)	HB101.....		pBM84	HAI::spt4 (Cys24; 27 to His) URA3 AmpR 2micron
FB1132	BBM89	Betsy Malone	NOTE: = Orientation I (H3 frag from mut17C into pCGS42.)	HB101.....		pBM89	HAI::spt4 (Cys24 to Ser) URA3 AmpR 2micron
FB1456	pBM30	Betsy Malone	RI-H3 spt4 frameshift from pBM28 cloned into RI-H3 of YCp50	HB101.n...		pBM30	spt4 frameshift URA3 CEN ARS ampR
FB2285	GC41	Bik Tye		HB101.....		GC41	"MCM1, Bluescript KS-
FB85		Bik Tye	"MboI paritals into Bam site of pCh1. Blue in haploid, white in diploid. "	HB101(?)..		pCh1-41	"amp, LEU2, lacZ, Ty
FB86		Bik Tye	see FB85	HB101?....		pCh1-51	see FB85
FB87		Bik Tye	see FB85	HB101?....		pCh1-103	see FB85
FB631		Biorad via Dana Boyd	see Biorad info (not in notebook)	.B101.....	dut- ung- thi- relA1	pCJ236	camR F' pox
FB2461	4N	Bob Kingston	for assembling a tetra-nucleosome array	?		4N
FB2460	3N	Bob Kingston	for assembling a tri-nucleosome array	?		3N
FB2459	2N	Bob Kingston	for assembling a di-nucleosome array	?		2N
FB2458	1N	Bob Kingston	For assembling a mono-nucleosome	?		1N
FB843	DH5alpha	Bob Moreland	good transformation strain	DH5alpha..	see notebook
FB846		Bob Moreland	80 amino acids	HB101.ha..		pfusA1-1	HTB2-lacZ
FB895		Bob Moreland	"see Cell 45, 537-544 "	HB101..E3)		pSKAdel8-7	HTA1-lacZ fusion
FB2301	pGN1101	Boeke Lab	SpeI/HindIII SPT10 genomic fragment in pRS415	epicurean.	ampR	pGN1101	"CEN, LEU2, SPT10
FB2806	pMAX175	Boeke Lab	Superloser		kanR URA3 URA3syn CEN ARS	pMAX175
FB1835	pRS401	Boeke lab		..........		pRS401	"ampR, Met15
FB1836	pRS411	BOEKE LAB		..........		pRS411	"ampR, MET15, CEN/ARS
FB1837	pRS421	BOEKE LAB		..........		pRS421	"ampR, Met15, 2micron
FB1838	pRS402	Boeke lab		..........		pRS402	"ampR, Ade2,
FB1839	pRS412	Boeke lab		..........		pRS412	"ampR, CEN/ARS, ADE2
FB1840	pRS422	Boeke lab		..........		pRS422	"ampR, Ade2, 2 micron
FB1841	pRS400	Boeke lab	no s.cerevisiae marker. KanMX is in opposite orientation from sequence provided by https://www.ncbi.nlm.nih.gov/nuccore/U93713.1. Confirmed by PCR using internal primers and corresponding flanking primers after integration into the genome	..........		pRS400	"ampR, Kanmx4neomycin (G418)
FB1842	pGC25	Boeke lab	no s. cerevisiae selectable marker	..........		pGC25	"ampR, CEN6/ARS4
FB1843	pGC26	Boeke lab	integrating marker	..........		pGC26	"ampR, 2 micron
FB2000	pJEF1908	Boeke lab		?.5a......		pJEF1908	"URA3, 2micron, CUP1-TY
FB758		Botstein	see notebook	.B101.....		pRB758	LEU2 polylinker
FB759		Botstein		.B101.....		pRB759	URA3 polylinker
FB1	DB6507	Botstein	Can screen for URA3 function	..........	pyrF74::Tn5 and all HB101 markers	none
FB6	RB18	Botstein		DB6656....	trp(am) lac(am) pyrF::Mu su-	YIp32	"LEU2, amp
FB7	RB19	Botstein	"Gene 8, 17-24 (1979) "	DB6656....	trp(am) lac(am) pyrF::Mu su-	YIp33	"LEU2, amp
FB8	RB20	Botstein	"Gene 8, 17-24 (1979) "	DB6656....	trp(am) lac(am) pyrF::Mu su-	YIp29	LEU2 URA3 amp
FB9	RB21	Botstein	"Gene 8, 17-24 (1979) "	DB6656....	trp(am) lac(am) su- pyrF::Mu	YIp28	LEU2 URA3 amp
FB20	DB6660	Botstein		DB6660....	trp(am) lac(am) pyrF::Mu hsr- hsm+ supF	none
DB21	RB39	Botstein	"ura3-18 on Bam fragment, LEU2 on Sal/Xho "	DB6660....	see FB20	pRB39	amp ura3-18 LEU2
FB2097	pKOSNF2L	Brad Cairns	snf2 knockout plasmid	DH5a......		pKOSNF2L	snf2D::LEU2
FB1854	MPY3xHA	Brandt Schneider	vector for HA tagging your favorite gene	DH5a......		MPY3xHA	"URA3, PUCori, ampR
FB1855	MPY3XMYC	Brant Schneider	Vector for MYC tagging your favorite gene	DH5a......		MPY3xMYC	"ampR, URA3, PUCori
FB2074	pITBL-SNF2	Brehon Laurent	SNF2 bromodomain deletion derivative	DH5a......		pITBL-SNF2	"SNF2(1-1554),URA3,2micron
FB2276	pLY50	Brehon Laurent	"(1990) MCB 10: 5616-5625, pLY50 is same as pLY14 "	DH5a......		pLY50	"4.8kb R1-BamH1 frag. from SNF5, pRS316
FB2277	pLY17	Brehon Laurent	(1990) MCB 10: 5616-5625	DH5a......		pLY17	"yIP5, snf5D2
FB2278	snf5-51ts	Brehon Laurent	single point mutation: E582K	DH5a......		snf5-51ts	"snf5-51ts, pRS306
FB2279	snf5-83b	Brehon Laurent	single point mutation D475N	DH5a......		snf5-83b	"snf5-83b, pRS306
FB2352	pLexA-SNF2	Brehon Laurent		XL1 Blue..		pLexA-SNF2	"2micron, HIS3, ADH1p-LexA-SNF2
FB2353	ADH1p-SNF2	Brehon Laurent		XL1 Blue..		ADH1p-SNF2	"2micron, LEU2, ADH1p-SNF2
FB2274	pYGFP3	Brendan Cormack	"Cormack et. al., (1997) Microbiology, 143:303-311 "	DH5a......		pYGFP3	"S65G, S72A mutations in GFP, super GFP
FB1263	BC9b	Brendan Cormack (Struhl)	*There is ANOTHER SmaI site in the pBR portion*	HB101.ha..		pBC9b	DED1 promoter GCN4 3'UT URA3 CEN ampR
FB1679	lexA-bic14	Brent Lab	Ref: Science 251:426-430	unknown...		Lex202-Bicoid-14	2micron HIS3 ampR lexA202-bicoid
FB1680	pRFHM202-C	Brent Lab	lexA(1-202)-CD12	unknown...		pRFHM202-CD12	HIS3 ampR lexA-CD12
FB1384	pSH2-1	Brent lab	REF: Cell 51:113; 1987 Cell 43:729; 1985 LexA polylinker vector	unknown.).		pSH2-1	ADH1promoter-lexA ampR 2micron HIS3
FB268		BRL	a useful plasmid	JM83......	ara del(lac-pro) thi strA phi80dlacZ delM15	pUC18	amp
FB269		BRL	Another useful plasmid	JM83......	ara del(lac-pro) thi strA phi80dlacZ delM15	pUC19	amp
FB285	TB1	BRL	For transformaing pUCclones into r-m+	TB1.1.....	ara del(lac pro) thi strA phi80dlacZ delM15 r-K m+K
FB1191	B1295	Broach Lab		unknown...		pB1295	MATa LEU2 CEN ARS ampR
FB1192	B1311	Broach lab	MATb (alpha) Hind3 cloned from LCIII 3a into YCp50	unknown...		pB1311	MATb URA3 CEN ARS ampR
FB1057	pKK5-1	Brosius .	"=plasmid for making RNA markers with E.coli RNA polymerase. Ref: Gene (1984 ) 27, p. 161-172 "	HB101.....		pKK5-1	AmpR
FB1058	pKK34-121	Brosius .	"plamid for making RNA markers wiht E. coli RNA polymerase. Ref: MGG (1985) 199, p. 55-58 "	HB101.....		pKK34-121	AmpR
FB1573	pdBE6	Brow lab	DO NOT GIVE OUT!! See enclosed sheets.	unknown...		pdBE6	snr6-dBE ampR
FB405	BD817	Bruce Duncan	"Gene 28, 211-219 (1984) "	BD817.....	ung1 nadB7 recA56 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 ga lT22 rpsL relA1(?)	none
FB406	BD1528	Bruce Duncan		BD1528....	ung1 nadB30 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 galT22 r psL relA1(?)	none
FB2086	pGAL-HO	Buratowski lab		S28.......		pGAL-HO	"URA3,LEU2,2micron,GALprom-HO
FB1289	RS426-TDS1	Buratowski.	TDS1 is a high copy suppressor of YEpGAL-IID[R105]. Vector= pRS426	unknown		pRS426-TDS1	TDS1 URA3 2micron ampR
FB2257	pACTII	C Brandl	2 hybrid	MH1.......		pACTII	"TRP1, 2 micron
FB1799	GHB98	C Kane Lab	linearized w/ PvuII-XhoI	DH5-alpha		pJD3	Amp ppr2d::HISG-URA3
FB2254	pSE1111	C. Brandl		MH1.......		pSE1111	"2micron, LEU2, SNF4
FB2255	pSE1112	C. Brandl		MH1.......		pSE1112	"2micron, TRP1, SNF1
FB2256	pAS1	C. Brandl	2-hybrid	MH1.......		pAS1	"TRP1, 2micron
FB536		C. Clark .	6kb SPT6 BglII fragment cloned into BamHI site of YEP24	HB101.....		pCC14	SPT6 (BglII frag) 2micron URA3 ampR
FB1445	p37/7	C. Connelly	see enclosed sheets	unknown...		p37/7	ctf4d3::URA3 ampR LEU2 CEN ARS
FB773		C. Denis	see notebook	HB101.....		pC1	CCR4 LEU2
FB989	pCHY23	C. Hoffman	BamHI-Nru1 of pCHY22 (lacZ) inserted into the XhoI site of pCHY21(FB748) Gen e 84:473-479 (1989)	HB101.....		pCHY23	ampR URA3
FB1796	GHB95	c. Kane lab		DH5-alpha.		pkc16(1-309)	TRP1 CRN Amp ARS PPR2
FB1797	GHB96	C. Kane Lab		DH5-alpha.		pkc13	GAL1-PPR2 2micron Amp
FB718		C. Lusty	see FB717	..........		YIp353	'lacZ
FB719		C. Lusty	see FB717	..........		YEp354	'lacZ
FB720		C. Lusty	see FB717	..........		YIp354	'lacZ
FB721		C. Lusty	see FB717	..........		YEp355	'lacZ
FB722		C. Lusty	see FB717	..........		YIp355	'lacZ URA3
FB723		C. Lusty	see FB717 and notebook	..........		YEp356	'lacZ URA3
FB724		C. Lusty	see FB717 and notebook	..........		YIp356	'lacZ URA3
FB725		C. Lusty	see FB717 and notebook	..........		YEp357	'lacZ URA3
FB726		C. Lusty	see FB717 and notebook	..........		YIp357	'lacZ URA3
FB727		C. Lusty	see FB717 and notebook	..........		YEp358	'lacZ URA3
FB728		C. Lusty	see FB717 and notebook	..........		YIp358	'lacZ URA3
FB729		C. Lusty	see FB717 and notebook	..........		YEp356R	'lacZ URA3
FB730		C. Lusty	see FB717 and notebook	..........		YIp356R	'lacZ URA3
FB731		C. Lusty	see FB717 and notebook	..........		YEp357R	'lacZ URA3
FB732		C. Lusty	see FB717 and notebook	..........		YIp357R	'lacZ URA3
FB733		C. Lusty	see FB717 and notebook	..........		YEp358R	'lacZ URA3
FB734		C. Lusty	see FB717 and notebook	..........		YIp358R	'lacZ URA3
FB1596	CP186	C. Peterson	SIN1=SPT2	unknown...		psin1dCR	sin1dCR URA3 ampR
FB1674	M105	C. Peterson	HO UAS= -1148 to -1488. Ref: Cell 68: 573 See also FB1675	unknown...		M105	URA3 CYC1-lacZ ampR HO UAS
FB1675	C769	C. Peterson	HO UAS= -769 to -1488 Ref: Cell 68: 573 See also FB1674	unknown...		C769	URA3 CYC1-lacZ ampR HO UAS
FB1687	BD1	C. Peterson	SWI1 in YEp24	DH5alpha..		BD1	SWI1 ampR
FB1075	CP32	C. Peterson	=BglII-SmaI frag of SWI3 in YIp5 (for making Southern probe). See letter in notebook.	unknown...		CP32	SWI3 URA3 AmpR
FB1076	CP34	C. Peterson	is in YEp24. SWI2 (SNF2) disruption allele was created by replacing the lg. BglII frag with HIS3 gene. See letter in notebook for information.	unknown...		CP34	swi2d::HIS3 AmpR URA3 2micron ori
FB1077	CP59	C. Peterson	see letter in notebook for information.	unknown...		CP59	swi3d::TRP1 AmpR URA3
FB1078	BD39	C. Peterson	"is in YIp5. swi1::LEU2 allele was constructed by replacing BamHI/BglII dele tion of SWI1 with LEU2. Digest with PstI, isolate ~1kb frag for one-step d isruption of SWI1. See notebook for letter. "	HB101.n...		BD39	swi1d::LEU2 AmpR URA3
FB685		Carl Falco	"Genetics 109, 21-35 (1985) "	HB101.....		pKD1-2	ILV2 YIp5
FB686		Carl Falco	"Genetics 109, 21-35 "	HB101.....		pKD3-4	YIp5 ilv2deletion
FB687		Carl Falco	see FB685	HB101.....		pBT177	ILV2 2 micron URA3
FB71		Carl Falco	Yip5+lys2 R1-H3 fragment	HB101.....		SL42-2	ura3 ampR lys2
FB72		Carl Falco	Yip5+lys2 R1-Cla1 fragment	HB101.....		SL46-1	ura3 ampR lys2
FB2495	pET28b-H2A	Carl Wu	Supercodon plus strain expressing H2A1	BL21		pET28B-H2A1	H2A1
FB2496		Carl Wu	H2B1 in supercodon plus strain	BL21		pET28b-H2B1	H2B.1
FB2497		Carl Wu	H3.2 supercodon plus strain	BL21		pET28b-H3.2	H3.2
FB2498		Carl Wu	H4.2 supercodon plus strain	BL21		pET28b-H4.2	H4.2
FB1499	pt32...	Carlson	REF: MCB 13: 3872 (1993) fig.1	unknown...		C1	msn2D3::HIS3 ampR
FB1500	pZfh45D2..	Carlson	DO NOT USE. M.CARLSON INFORMED US ON JULY 96 THAT THIS CONSTRUCT IS WRONG. REF: MCB 13:3872 (1993) FIG.4.	unknown...		D7	msn4D2::URA3 ampR
FB1501	pEB312D1..	Carlson	REF: NAR 18:6959 (1990)	unknown...		I2	msn1::URA3 ampR
FB1502	pJH104	Carlson	REF: MCB 14:1972 (1994) fig.1	unknown...		G9	msn3D2::HIS3 ampR
FB1510	pEY32H	Carlson	REF: MCB 13:3872 (1993) fig.1	unknown...		pEY32H	MSN2 ampR 2micron URA3
FB1511	pEL45	Carlson	REF: MCB 13:3872 (1993) fig. 4	unknown...		pEL45	MSN4 ampR 2micron URA3
FB1512	pJH68	Carlson	REF: MCB 14:1972 (1994) fig. 1	unknown...		pJH68	MSN3 2micron ampR URA3
FB1513	H7.5	Carlson	REF: MCB 14: 1972 (1994) page 1973	unknown...		H7.5	MTH1 2micron URA3 ampR
FB1589	pJH124	Carlson lab	for MTH1 gene knockout-- use EcoRI digest. See enclosed sheets.	HB101.lKC.		pJH124	mth1d::URA3 ampR
FB475	CAG10153	Carol Gross	This is a nonsense rpoH allele. Grow the strain at 30 or 35. Ts best seen at 42. See notebook for details.	CAG10153..	MC1061 rpoH165 zhf50::Tn10	none
FB476	CAG1578	Carol Gross	"Ts rpoD allele with linked Tn10. 25-30 is permissive, 42 is nonpermissive. Seenotebook for details. "	CAG1578...	MC1061 rpoD800 Tn10	none
FB717		Carol Lusty	"Gene 45, 299-310; also see FB717-734; FB798-815 "	..........		YEp353	'lacZ
FB798		Carol Lusty	"Gene 45, 299-310; see FB717 for URA3 versions; see notebook; see 798-815 "	HB101.....		YEp363	lacZ LEU2
FB799		Carol Lusty	see FB798	HB101.....		YIp363	lacZ LEU2
FB800		Carol Lusty	see FB798 and notebook	HB101.....		YEp364	lacZ LEU2
Fb801		Carol Lusty	see notebook and FB798	HB101.....		YIp364	lacZ LEU2
FB802		Carol Lusty	see notebook and FB798	HB101.....		YEp365	lacZ LEU2
FB803		Carol Lusty	see notebook and FB798	HB101.....		YIp365	lacZ LEU2
FB804		Carol Lusty	see notebook and FB798	HB101.....		YEp366	lacZ LEU2
FB805		Carol Lusty	see notebook and FB798	HB101.....		YIp366	lacZ LEU2
FB806		Carol Lusty	see notebook and FB798	HB101.....		YEp367	lacZ LEU2
FB807		Carol Lusty	see notebook and FB798	HB101.....		YIp367	lacZ LEU2
FB808		Carol Lusty	see notebook and FB798	HB101.....		YEp368	lacZ LEU2
FB809		Carol Lusty	see notebook and FB798	HB101.....		YIp368	lacZ LEU2
FB810		Carol Lusty	see notebook and FB798	HB101.....		YEp366R	lacZ LEU2
FB811		Carol Lusty	see notebook and FB798	HB101.....		YIp366R	lacZ LEU2
FB812		Carol Lusty	see notebook and FB798	HB101.....		YEp367R	lacZ LEU2
FB813		Carol Lusty	see notebook and FB798	HB101.....		YIp367R	lacZ LEU2