Bacteria

Displaying 1 - 500 of 2807

Strain Name	Strain Alias	Source	Comments	Host	Host Markers	Plasmids Sort descending	Plasmid Markers
				fb2195
	pms4
pms4
FB2516



FB2114
%FB2114%

fb2044
	dh5a
	dh5alpha
fb843
fb843
	dh5a
FB2691	DH10Bac	Invitrogen		DH10Bac			bacmid pMON4274-kan helper plasmid pMON7124-tet
FB2692	pFastBac1	Invitrogen		DH5a			Ampicillin-R Gentamicin-R
FB2693	FastBc1GUS	Invitrogen	pFastBac1-GUS	DH5a			Ampicillin-R Gentamicin-R

fb603
FB2687	pZM467	Struhl Lab	Struhl Lab. Yeast 2008: Volume 25; 287-292.				3xFLAG URA3 3xFLAG
FB2688	pZM473	Struhl Lab	Struhl Lab. Yeast 2008: Volume 25; 287-292.				3xHSV URA3 3xHSV
FB2689	pZM474	Struhl Lab	Struhl Lab. Yeast 2008: Volume 25; 287-292.				3xV5 URA3 3xV5
FB2690	pZM475	Struhl Lab	Struhl Lab. Yeast 2008: Volume 25; 287-292.				VSV-G URA3 VSV-G
fb2697	CKB200		EcoRI-BamHI fragment of pCK198 ligated into BamHI-EcoRI digested pRS306. For two-step integration of GAL10 poly-A+ site mutant.	DH5a			ampr, ColE1 ori, pRS306 backbone (URA3/ARS)
FB

FB
fb
FB2699	pEL183	FB2338	FB2338 except with spt6-50 corrected so that it's WT SPT6 pRS414 backbone, SPT6-FLAG, TRP1, CEN, ARS (Constructed from FB2338, pCK134)	DH5alpha	SPT6-FLAG		CEN ARS TRP1 ampR

FB2708		ApoLife, Nalini Motwani	Do not give out.	DH5a			pM40-3 HPAIX AmpR
FB2709		ApoLife, Nalini Motwani	Do not give out	DH5a			pMC50-2 AmpR
FB2730	Stbl3	Invitrogen	Life Technologies Corporation Catalog #C737303 Lot# 1649448	Stbl3
FB2746	A02V	Michael Springer Lab	Derived from pFA6-kanMX. pFA6-HO-TDH3-YFP-ADH1-pTEF-kanMX-TEF-HO		ampR		pFA6-HO-TDH3-YFP-ADH1-pTEF-kanMX-TEF-HO
FB2747	A03V	Michael Springer Lab	Derived from pFA6-kanMX		ampR		pFA6-HO-TDH3-mCherry-ADH1-pTEF-kanMX-TEF-HO
FB2748	A05V	Michael Springer Lab	Derived from pFA6-natMX		ampR		pFA6-HO-TDH3-YFP-ADH1-pTEF-natMX-TEF-HO
FB2749	A06V	Michael Springer Lab	Derived from pFA6-natMX		ampR		pFA6-HO-TDH3-mCherry-ADH1-pTEF-natMX-TEF-HO
FB2783	pBK257		SATAY method. ADE2 is not expressed until transposon hops, so yeast transformants will be Ura+ Ade-	DH5a	AmpR		CEN ADE2 URA3 TPase(103-807)4x
FB2817	RGBO5	Beate Schwer					TRP1 CEN ampR CEG1
FB2818	RGBO6	Beate Schwer					TRP1 CEN ampR ceg1-3
FB2819	RGBO7	Beate Schwer					TRP1 CEN ampR ceg1-13
FB2820	RGBO8	Beate Schwer					TRP1 CEN ampR ABD1
FB2821	RGBO9	Beate Schwer					TRP1 CEN ampR abd1-5
FB2822	RGBO10	Beate Schwer					TRP1 CEN ampR abd1-8
FB2823	CE113	Stephen Buratowski					HIS3 CEN ampR CET1
FB2824	CE333	Stephen Buratowski					HIS3 CEN ampR cet1-401
FB2825	CE339	Stephen Buratowski					HIS3 CEN ampR cet1-438
FB2797	pZiDSK2	Steve Doris		DH5a			AmpR LEU2 CEN Z3EV-iDSK2
FB1585	JM107----	S. Elledge	Full name of HOST= JM107/lambdaKC (is the host strain used for Steve Elledg e lambda library (10X/cre sys) See enclosed sheets.	JM107/lKC.	KanR lambda lysogen cre lacI
FB1611	BB778	J. Boeke	"CAUTION"" DID NOT CONFER CANAVININE RESITANCE TO can1 MUTANT CELLS"	" 9/99	unknown...		pRS319
FB1614		Golderg .	good host for expressing foreign proteins in bact.	LC137.n...	htpR
FB1615		Golberg .	Good host for expressing foreign proteins in bact.	Clp-	Clp-
FB1645	K802	Grant Hartzog		K802......	F- e14- (McrA-) lacY1 or d(lac)6 supE44 galK2 galT22 rfbD1 metB1 mcrB1 hsdR2 (r-k M+k)
FB1659	PLKF'	Y. Kohara .	"for M13 rescue of lamda zap, general M13 work. See enclosed sheet. "	PLKF'	"e14-(mcrA) mcrB1 lac recA hsdR2 (rK-,mK-) supE44 galK2 galT22 metB1 F' proAB laclqZDM15 TN10(tetR
FB1688	C600	T. Lane (Leder lab)	original C600 isolate was EcoK r+m+ McrBC+ See also BNN93 with enclosed s heet in notebook	C600......	F- e14- (McrA-) thr-1 leuB6 thi-1 lacY1 supE44 rfbD1 fhuA21 EcoK r+m+ McrBC+
FB1986	pDR14	Dave Rose		.H5a......
FB1140		A. Rowley + Curt Wittenburg		HB101.....
FB1172	MH1	Lorraine Marsh	*Good for transformation with Smash and Grab DNA* see enclosed sheet in notebook for transformation procedure.	MH1.1.....
FB1260	RZ1032	B. Berg	for dut ung mutagenesis	RZ1032....	dut1 ung1 thi1 relA HfrKL16 PO/45 [lysA61-62] 2bd-279::Th10 supE44
FB1266	GAL1-cDNA	Tony Bretscher	GAL1-cDNA library REF: Genetics 132 (1992) p. 665-673	DH10B			GAL1-cDNA library
FB10	SF1032	Froshauer	MC4100 genetic background	SF1032....	srl::Tn10 reca ara relA lac(del) strR
FB81	LG90	Guarante	"Guarante and Ptashne PNAS 78,2199-2203 "	LG90.7....	F- dellacproXIII
FB106	B621	Collaborative	"GAL UAS on 0.75 kb fragment, Xho cloning site "	?.........			GAL UAS URA3 amp
FB107	B622	Collaborative	GAL UAS with Sal cloning site	?.........			"GAL UAS, URA amp 2 micron
FB112	HB101	via Fink	B324 in GR Fink collection	.A300.....	thr leu pro B1 supE44 r- m- recA
FB113	B522	Silhavy	"PNAS 80,4432 "	MH3000....	"araD139 del(ara,leu)7697 del(lac)X74 galU galK rpsL (strR) ompR101
FB114	B523	Silhavy		TK1046....	araD139 del(argF-lac)U169 rpsL150(strR) relA1 flbB5301 deoC1 ptsF25 ma lPQ::Tn5 ompBcs1
FB116	K38			K38.46....	HfrC
FB178		Christy		HB101.....			spt3-233
FB179		Christy		HB101.....			spt3-240
FB180		Christy		.B101.....			spt3-242
FB181		Chirsty		HB101.....			spt3-242
FB182		Christy		HB101.....			spt3-243
FB183		Christy		HB101.....			spt3-244
FB184		Christy		HB101.....			spt3-245
FB185		Christy		HB101.....			spt3-246
FB186			deleted	.B101.....
FB187		Christy		HB101.....			spt3-248
FB188		Christy		HB101.....			spt3-249
FB189		Christy		.B101.....			spt3-250
FB190		Christy		HB101.....			spt3-251
FB191		Christy		HB101.....			spt3-252
FB192		Christy		HB101.....			spt3-253
FB193		Christy		.B101.....			spt3-254
FB194		Christy		HB101.....			spt3-255
FB195		Christy		HB101.....			spt3-256
FB196		Christy		HB101.....			spt3-257
FB197		Christy .		HB101.....			spt3-258
FB198		Christy		HB101.....			spt3-259
FB199		Christy		HB101.....			spt3-260
FB200		Christy		HB101.....			spt3-261
FB236	RR1	T.J. Koerner and A. Tzagaloff	Other markers not known; probably rec+. Host for pATH vectors for trpE fusi ons.	RR1.07....	r- m-
FB237	RR1delM15	Lothar	host for pUR290-2 plasmids	RR1delM15.	r- m- lacZalpha deletion
FB274	MM572	Martin Marinus	"dam, to make DNA cuttable by certain enzymes such as BclI "	GM237.....	dam-3 recA1 sin-1 thr-1 leuB6 proA2 metB1 thi1 deoB16 ara-14 lacY1 gal K2 rspL260 tsx33 supE44 mut4
FB284		R. Surosky	Beware of this plasmid. May be incorrect.	HB101.....			his4-912delta
FB285	TB1	BRL	For transformaing pUCclones into r-m+	TB1.1.....	ara del(lac pro) thi strA phi80dlacZ delM15 r-K m+K
FB298		Heffron	see notebook	NS2114....
FB326	MC1065	M. Casadaban	can use to check yeast TRP1	MC1065....	F- galE15 galK16 dellacX74 (pro+) leuB6 trpC9830 strA(rspL) B1-
FB351	LM1035	Connie Cepko	derivative of HB101; supposed good transforming strain	LM1035....	same as HB101
FB352	AR68	SmithKline	good for production of hybrid proteins; see notebook	AR68.5....
FB910		Tricia Davis		.B1 ..n...
FB911		Tricia Davis		AB1886	F- uvrA6 thr1 leu6 thi1 lacV1 galkz ara14 xyl5
FB923		Dave Deitcher		TG1.own...	"JM101, Ecok
FB1323	DH10/P3	Grant Hartzog	E. coli strain for propagating supF plasmids	DH10/P3...	"F- mcrA d(mrr-hsd RMS-mcrBC) 0 80d lacZdM15 dlacX74 deoR recA1 arad139 a(ara, leu)7697 galu galk
FB1359	XL1-Blue	Stratagene	can use for M13 helper phage preps. See enclosed sheets in notebook.	XL1-Blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F` proAB lacIq ZdM15 tn10 tet-]
FB1439	GM48	G. Duyk	contains NO dam or dcm methylases (from NEB catalogue)	GM48	F- thr leu thi lacY galK ara fhuA tsx dam dcm SUPE44
FB2130	JMB76	L.Guarent	SpTBP in pRS426 Nature 346:291-294	DH5a......			"2micron,URA3 SpTBP
FB2144	JMB210	L.Stargell/Struhl	Science 269:75-78	DH5a.DE3..			SPT15 modified
FB2145	JMB211	L.Stargell/Struhl	Science 269: 75-78	DH5a.DE3..			TOA1-SPT15
FB2146	JMB212	L. Stargell	Science 269:75-78	DH5a......			TOA2-SPT15
FB2181		David Stillman	"resistant to killing by DNA gyrase, for use with pRS416-0 "	NH975.....
FB2182		Beckwith Lab		DHB4......
FB2701		Mei Sheng/Niroshi Senaratne	Generated by Mei Sheng during her rotation by inserting URA3 into LacZ in the pRS313 Myc-SPN1 HIS3 CEN AmpR plasmid received by Erin Loeliger from the Stargell Lab. This plasmid containing URA3 was extracted from yeast by Niroshi Senaratne and transformed into E. coli, here stored as FB2701.	DH5a			HIS3 URA3 Myc-SPN1 AmpR CEN
xit
		rothstein
FB
							ura3 his3
							snf1
							ura3
							%FLAG%

		Joel
							hml
							%hml%

FB2492	BL21/DE3	Rolf Sternglanz	Super codon plus strain for protein expression	BL21/DE3	Chloramphenicol
FB432	NK5772	Nancy Kleckner	See notebook about checking markers by 2-aminopurine test.	NK5772....	dcm-6 dam-3 metB1 galK2 galT22 lacY1 tsx-78 (thi-1 tonA32 mtl-1)? prob ably an amber suppressor str
FB439			DO NOT USE	.B101.....
FB440			DO NOT USE	.B101.....
FB485				.B101.....
FB605		Stan Fields	lacZ library; see notebook	MC1066....	del(lac)X74 hsr- hsm+ strA trpC9830 leuB600 pyrF::Tn5 galU galK		fusion library to lacZ
FB606		Stan Fields	lacZ fusion library; see FB605 and notebook	MC1066....			lacZ fusion library
FB607		Stan Fields	lacZ fusion library; see FB605 and notebook	MC1066....	see FB605		lacZ fusion library
FB664		T. St. John via K. Struhl	see notebook; GAL probe	HB101.e...			pUC8 GAL UAS 4812
FB665		T. St. John via K. Struhl	"see notebook, GAL probe "	HB101.e...			pUC8 GAL fragment 4902
FB735		B. Daignan-Fornier	This strain is for making MuLEU2 hops; see notebook	JM109.....	JM109 MuCts ZZ4 CAMR
FB736		B. Daignon-Fornier	see notebook	M8820.....
FB777		M.A. Osley	Exact structure unknown	HB101.....			pBL101 H2B2-lacZ
FB782				.B101.....
FB843	DH5alpha	Bob Moreland	good transformation strain	DH5alpha..	see notebook
FB597		D. Ruden and M. Ptashne		HB101.....		"LR1del(25,25,24)	GAL; see notebook
FB596		D. Ruden and M. Ptashne		HB101.....		"LR1del(25-25,23)	GAL; see notebook
FB426		N. Kleckner	duplicate with FB425	NK5830....	see FB415	"pFW127, pNK629	see notebook
FB2458	1N	Bob Kingston	For assembling a mono-nucleosome	?		1N
FB1636	GHB54	Hartzog via Soares	from image consortium Genbank#= T80145	DH10B.ha..		24522	ampR lafmidBA potential human SPT5 cDNA
FB1234		Nasmyth	2micron LEU2 library in YEp13 (2 vials frozen)	unknown...		2micron LEU2 library	2micron LEU2 library
FB2459	2N	Bob Kingston	for assembling a di-nucleosome array	?		2N
FB1637	GHB55	Hartzog via Soares	from image consortium (nearly identical to 24522=FB1636) Genbank#= R14898	DH10B.ha..		30004	ampR lafmidBA potential human SPT5 cDNA
FB1638	GHB56	Hartzog via Soares	from image consortium Genbank#= R18289	DH10B.ha..		30813	ampR lafmidBA potential human SPT5 cDNA
FB2460	3N	Bob Kingston	for assembling a tri-nucleosome array	?		3N
FB2502	3xHA-TBP	Kevin Struhl		DH5a		3xHA-TBP	3xHA-TBP URA3 CEN
FB2461	4N	Bob Kingston	for assembling a tetra-nucleosome array	?		4N
FB2816	bJLW50	Maozed or Luger Lab via Buratowski	From Buratowski Lab "Plasmid carries 12 copies of Widom 601 positioning ssequence which can be released as 147 bp fragments with EcoRV. Note: insert is easily recombined, so grow in the STBL3 cells and keep an eye out for deletions"	STBL3		601 x 12	AmpR, 12xWidom601
FB109	B626	D. Schild via Fink	see FB108	JA300.....	see 108	?	rad52::LEU2
FB647		Christine	in frame fusion	RR1.......		A	pATH3 SPT8 Bam-Bam
FB2083		Berger Lab	F to A mutant	DH5a......		A/C FA	gal4-VP16 LEU2 CEN
FB2082		Berger Lab		DH5a......		A/C GV	"gal4-VP16 Leu2, CEN
FB2451	A1-1	Erica Larschan	Rose Library Clone	DH5a		A1-1 SRB9-YCP50	URA3 CEN SRB9
FB2452	A2-2	Erica Larschan	Rose Library Clone	DH5a		A2-2 SRB9 Ycp50	SRB9 URA3 CEN
FB1963	A2Nde	Guarente Lab		DH5a......		A2Nde	"CEN, Leu2, ADA2
FB965	AB289	Kim Arndt .	See enclosed sheet DO NOT GIVE OUT! See Science Aug87 vol.237 p.874; Nov89 vol.246 p.931	..........		AB289	URA3 ampR ori
FB966	AB328	Kim Arndt .	See enclosed sheet DO NOT GIVE OUT! See Science Aug87 vol.237 p.874; Nov.8 9 vol.246 p.931	..........		AB328	URA3 ampR ori
FB2046	ADA1KO	Guarente Lab	MCB 17:3220-8 (1997)	DH5a......		ADA1KO	"ADA1, hisG, URA3
FB2353	ADH1p-SNF2	Brehon Laurent		XL1 Blue..		ADH1p-SNF2	"2micron, LEU2, ADH1p-SNF2
FB80		J. Abraham via Bik Tye	H3 fragment of MATalpho into the H3 site of YEp13	DB6507....		alpha*7.375	MATalpha LEU2 2 micron
FB118	B155	"Fink, Phil Carter	orientation of insert unknown	?.101.....		B155	Ty912 HIS4 URA3 amp
FB92	B161	Fink	"Ty probe, internal "	?.6507....		B161	"amp, Ty 1.2 BglII fragment
FB318	B163	R. Surosky and G.R. Fink	see notebook for exact map of fragment	?.101.n...		B163	ampR Ty S13 BglII fragment
FB967	B238	Hinnebusch.	See enclosed sheet for references! DO NOT GIVE OUT!	..........		B238	URA3 CEN ARS ampR ori GCN4
FB1998	B2616	Fink Lab		DH5a......		B2616	"ampR, pBRori, URA3, STE11-4, CEN
FB649		Christine	out of frame fusion	.R1.......		B3	pATH2 SPT8 Bam-Bam
FB645		Christine	in-frame fusion	RR1.......		B4	pATH2 SPT7 Bgl2-Bam
FB968	B401	Hinnebusch.	See enclosed sheets. DO NOT GIVE OUT!	..........		B401	URA3 CEN ARS gcn4::LEU2 ampR ori
FB1687	BD1	C. Peterson	SWI1 in YEp24	DH5alpha..		BD1	SWI1 ampR
FB1374	Bd1128	G. Mikesell	= pGAL::HIS3 in YCp50 REF: Genes + Dev. 5:1183-1190	HB101.n...		Bd1128	pGAL::HIS3 ampR CEN ARS URA3
FB1078	BD39	C. Peterson	"is in YIp5. swi1::LEU2 allele was constructed by replacing BamHI/BglII dele tion of SWI1 with LEU2. Digest with PstI, isolate ~1kb frag for one-step d isruption of SWI1. See notebook for letter. "	HB101.n...		BD39	swi1d::LEU2 AmpR URA3
FB1375	Bd824	Mikesell	=pGAL::SWI4 in YCp50 REF: Genes and Dev. 5:1183-1190	HB101.....		Bd824	pGAL::SWI4 URA3 CEN ARS ampR
FB1995	BHM270	Fink Lab		?.5a......		BHM270	"ampR, ori, STE11-4, LEU2, CEN
FB1996	BHM271	Fink Lab		?.5a......		BHM271	"ampR, ori, CEN, HIS3, STE11-4
FB1997	BHM406	Fink Lab		?.5a......		BHM406	"ori, ampR, STE11-4, CEN, TRP1
FB2081	BJC33	Joan Curcio	in Cla adapter plasmid which is based on pBR327	MH1.......		BJC33	HIS3AI
FB2807	bJLW2	JLW	SPT6 cloned into Superloser plasmid	DH5a	Spn1 Plasmid from Grant Hartzog Lab	bJLW2	KanR, URA3, URA3syn, CEN/ARS
FB620		Stratagene	"Bluescript KS polylinker, see notebook "	.B101.....		Bluescript m13+	KS polylinker Bluescript
FB621		Stratagene	Bluescript	.B101.....		Bluescript m13+	"SK polylinker, Bluescript
FB622		Stratagene	"bluescript, see notebook "	.B101.....		Bluescript m13-	"KS polylinker, bluescript
FB623		Stratagene	"bluescript, see notebook "	.B101.....		Bluescript m13-	"SK polylinker, bluescript
FB2246	BM3076	Mark Johnston		DH5a......	'	BM3076	"CEN, URA3, 3XMyc::MIG1
FB2802	RGB44	Rajaraman Gopalakrishnan		DH5a		bMON14272-GST-SET2	AmpR GentR TetR GST-SET2
FB2803	RGB46	Rajaraman Gopalakrishnan		DH5a		bMON14272-GST-SET2-H366N	AmpR GentR TetR GST-SET2-H366N
FB2772	BYP6739	NBRP	pCUP1-IAA17, kamMx			BYP6739	AmpR KanR
FB2773	BYP6740	NBRP	IAA17, KanMx			BYP6740	AmpR KanR
FB2774	BYP7430	NBRP	3xminAID-KanMx			BYP7430	AmpR
FB2775	BYP7431	NBRP	3xminAID -hphNP			BYP7431	AmpR
FB2776	BYP7432	NBRP	3xminAID -His3Mx	DH5a		BYP7432	AmpR
FB2777	BYP7433	NBRP	3xminAID -natNT	DH5a		BYP7433	AmpR
FB1499	pt32...	Carlson	REF: MCB 13: 3872 (1993) fig.1	unknown...		C1	msn2D3::HIS3 ampR
FB1675	C769	C. Peterson	HO UAS= -769 to -1488 Ref: Cell 68: 573 See also FB1674	unknown...		C769	URA3 CYC1-lacZ ampR HO UAS
FB2778	C81B	Springer Lab, HMS	HO-pTDH3-mcherry-Hgh-HO			C81B	AmpR mCherry
FB963	CB100	Kim Arndt .d	See enclosed sheet DO NOT GIVE OUT! See Science Aug87 vol.237 p.874; Nov89 vol.246 p.931	unknown		CB100	BAS2 ampR ori
FB964	CB2	Kim Arndt .	BAS1 inserted into pUC118. See enclosed sheet DO NOT GIVE OUT! See Scienc e Aug87 vol.237 p.874; Nov89 vol.246 p.931	unknown		CB2	BAS1 ampR
FB1856	GHB111	Karen Arndt	"Ref. MCB 11, 2133 (1991) "	MH1.......		CB852	SSD1-V in Ycp50
FB1857	GHB112	Karen Arndt	"use BamH1/Sph1 to integrate, ref. MCB 11, 2133 (1991) "	MH1.......		CB889	ssd1d::LEU2
FB105	B620	Collaborative	GAL UAS on 0.75 kb fragment; does NOT contain the GAL +1 of transcription	.B101?....		CGE258	amp URA3 2 micron GAL UAS
FB2795	CKB112	Craig Kaplan	pRS414 backbone	DH5a		CKB112	TRP1 CEN ARS ampR SPT6-FLAG DN122
FB2794	CKB113	Craig Kaplan	pRS414 backbone	DH5a		CKB113	TRP1 CEN ARS ampR SPT6-FLAG DN205
FB2792	CKB30	Craig Kaplan	pRS414 backbone	DH5a		CKB30	TRP1 CEN ARS ampR SPT6-FLAG
FB2793	CKB84	Craig Kaplan	pRS414 backbone	DH5a		CKB84	TRP1 CEN ARS ampR SPT6-FLAG DN86
FB1075	CP32	C. Peterson	=BglII-SmaI frag of SWI3 in YIp5 (for making Southern probe). See letter in notebook.	unknown...		CP32	SWI3 URA3 AmpR
FB1076	CP34	C. Peterson	is in YEp24. SWI2 (SNF2) disruption allele was created by replacing the lg. BglII frag with HIS3 gene. See letter in notebook for information.	unknown...		CP34	swi2d::HIS3 AmpR URA3 2micron ori
FB1077	CP59	C. Peterson	see letter in notebook for information.	unknown...		CP59	swi3d::TRP1 AmpR URA3
FB317		D. Garfinkel	high copy number suppressor	HB101.n...		CS14	ampR; high copy SPT23
FB2779	D14B	Springer Lab, HMS	HO-pTDH3-BFP2-Hgh-HO			D14B	AmpR BFP2
FB1406	D16pUC13+	MaryAnn Osley	=deletion 16prime (D16prime) with a XhoI site *SalI site is gone* =TRT 1 promoter with negative element deleted (region is marked by URA3+ insert ed downstream of H2B	HB101.e...		D16pUC13+	D16prime ampR
FB646		Christine	out-of-frame fusion	RR1.......		D2	pATH1 SPT7 Bgl2-Bam
FB1500	pZfh45D2..	Carlson	DO NOT USE. M.CARLSON INFORMED US ON JULY 96 THAT THIS CONSTRUCT IS WRONG. REF: MCB 13:3872 (1993) FIG.4.	unknown...		D7	msn4D2::URA3 ampR
FB2021	DC92	MARIAN OSLEY LAB		DH5A......		DC92	WT HTA1 AND WT HTB1 YEP352 =URA3
FB752		Lenny Guarente	Deletion removes all TATAs and the first transcription initiation site. All other initiation sites remain.	HB101.....		del-178/+3	CYC1-lacZ URA3 amp
FB2106	pCK17	Craig Kaplan		?.5a......		DmSPT5-ESTD08338	pBluescript KS - Backbone
FB648		Christine	out of frame fusion	.R1.......		E2	pATH1 SPT8 Bam-Bam
FB2266	Erp50L1	Heng Zhou		DH5a......		Erp50L1	"Ura3, 2micron, Nrg1
FB651	MC1000	Dana Boyd	requires leu + ile to grow on minimal medium	MC1000....	malFdel3 phoA delphe phoR-	F'	F' lacpro lacIq camR
FB5	TR5673	J.R. Roth	grows in histidinol	TR5695....	trp B1 his (P2 eductant)	F' hisG+D+	hisG+D+
FB157	JM103		"Find markers in most catalogs, eg NE Biolabs, BRL; M13 host "	JM103.....		F'pro
FB1483	FAT-RS303b	Gottschling	see enclosed sheets	MC1066....		FAT-RS303`b'	ampR HIS3 2micron ARS leu2-d
FB2084		Berger Lab		DH5a......		G/V	"2micron Gal4-VP16, LEU2
FB1502	pJH104	Carlson	REF: MCB 14:1972 (1994) fig.1	unknown...		G9	msn3D2::HIS3 ampR
FB1806		M. Ptashne	see book	MH1.alpha.		gal11wt 2u Myc93	myc-gal11 2 micron LEU2
FB1590	GAL4-VP16	M. Carey	Ref: Chasman et al MCB 9:4746 (1989) See enclosed sheet.	XA90..lKC.		GAL4-VP16	GAL4-VP16 ampR
FB2285	GC41	Bik Tye		HB101.....		GC41	"MCM1, Bluescript KS-
FB2796	GHB346	Hartzog Lab	GHB346 with pRS316. Plasmid GHB242	DH5a		GHB346	Ura3 AmpR SPN1
FB2128	h/hp77B1	J.Madison		DH5a......		h/hp77B1	"URA3,CEN, wt hTBP in pRS316
FB2761	hH2B	genewiz	yeast optimized human H2B. Amplify with H2B-HR F and H2B-HR R for integration at URA3	Mh1		H2AFx	Kan LacZ
FB1513	H7.5	Carlson	REF: MCB 14: 1972 (1994) page 1973	unknown...		H7.5	MTH1 2micron URA3 ampR
FB2033	GHB76	Nomura Lab		DH5a......		HA3982	"amp, SUPT6H
FB1470	hbc211	Jun Takeda	*DO NOT DISTRIBUTE!!* = human SPT4 homologue REF: Human Molec. Genet ics 2(11) 1793 (1993)	SOLR......		hbc211	ampR hSPT4 homologue
FB1989				.L-1 Blue.		HDF1D::TRP1	TRP1 HDF1D
FB2717		Arndt Lab	Derived from pWZ414-F12 (Zhang et al EMBO J. 1998 June1; 17(11):3155-3167. From Shilatifard Histone Mutant Library. Obtained from Arndt Lab.	DH5a		hht2-K36A-HHF2	hht2-K36A-HHF2 TRP1 CEN3
FB2337	His3GALGFP	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		His3MX6-PGAL1-GFP	"His3MX6, GAL1prom, GFP(S65T), ADH1term
FB2334	His3GALGST	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		His3MX6-PGAL1-GST	"His3MX6, GAL1prom, GST, ADH1term
FB2760	hH2A.X	genewiz	yeast optimized human H2A.X. Amplify with H2A.X-HR F and H2A.X HR F for integration at URA3	Mh1		HIST1H2Be	Kan LacZ
FB1501	pEB312D1..	Carlson	REF: NAR 18:6959 (1990)	unknown...		I2	msn1::URA3 ampR
FB2382	JK0024	Jerry Kaplan		DH5a......		JK0024	URA3 2micron VPS41
FB68	JT503	J. Thorner	RI-H3 of MATa	DB6507....		JT503	MATa
FB2335	kanGALGFP	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		kanMX6-PGAL1-GFP	"kanMX6, GAL1prom, GFP(S65T), ADH1term
FB2332	KanGAlGST	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		kanMX6-PGAL1-GST	"kanMX6, GAL1prom, GST, ADH1term
FB1165	L15/pUN45	S. Buratowski	SPT15 with Lerner at N terminal in vector pUN45 (Elledge + Davis)	HB101.....		L15/pUN45	SPT15 with Lerner epitope at NT TRP1 CEN ARS ampR
FB1166	L15/pUN55	S. Buratowski	spt15 with Lerner at N terminal in vector pUN55	HB101.....		L15/pUN55	SPT15 with Lerner epitope at NT URA3 CEN ARS ampR
FB2771	L260	Leon Chan Berkeley (Koshland)	IAA7-3xV5, KanMx			L260	AmpR KanR
FB22	B28lam322	Ralph Isberg		B28.60....	see FB4	lambda 322	b5a5 cI857 nin Sam7 Tn903(b2)
FB1681	lex202+B42	Ptashne Lab	lexA(1-202)-acid blob B42	unknown...		lex(202+B42)	HIS3 ampR 2micron lexA-B42
FB1679	lexA-bic14	Brent Lab	Ref: Science 251:426-430	unknown...		Lex202-Bicoid-14	2micron HIS3 ampR lexA202-bicoid
FB1301	LR1d1	Roger Brent	GAL1 deleted UAS leaves XhoI site. NO LexA ops. this strain is the negati ve control for FB1302-FB1306.	DH5 (?).		LR1d1	GAL1dUAS-lacZ URA3 ampR 2micron
FB1676	LR1d1dSpe	Lena Wu	integrating version of LR1d1 (FB1301). 2micron was cut out on SpeI frag.	DH5alpha..		LR1d1dSpe	ampR URA3 GAL1-10dUAS lacZ integrating
FB594		D. Ruden and M. Ptashne		HB101.....		LR1del23	GAL; see notebook
FB595		D. Ruden and M. Ptashne		HB101.....		LR1del24	GAL; see notebook
FB625		Ruden and Ptashne	see FB592 and notebook	HB101.....		LR1del24T-	GAL lac TATA test plasmid
FB624		Ruden and Ptashne	see FB592 and notebook	HB101.....		LRadel24T+	GAL lacZ TATA test plasmid
FB592		Roger Brent	"see notebook and West et al MCB 4, 2467 "	.B101.....		LRdel20B	GAL1-lacZ with Xho site between UAS and TATA
FB1674	M105	C. Peterson	HO UAS= -1148 to -1488. Ref: Cell 68: 573 See also FB1675	unknown...		M105	URA3 CYC1-lacZ ampR HO UAS
FB2392	M1063	David Stillman		DH5a......		M1063	"HO, HIS3, pBR322
FB1807		D. Stillman	see book	MH1.alpha.		m1305	Sin+ 2micron URA3
FB1808		D. Stillman		MH1.alpha.		m1368	Sin4 TRP1 disruptor
FB1817	M1391	D. STILLMAN	high copy SIN4	DH5a......		M1391	ADH-SIN4 LEU2 2MICRON AMP
FB1812	M1889	D. Stillman		DH5a......		M1889	"lexA, SIN4, His3
FB1815	M1967	D. Stillman	GST-RGR1 IN pRS316	DH5a......		M1967	CEN GAL-GST-RGR1 URA3 AMP
FB1814	M1968	D. Stillman	GST-SIN4 in pRS316	DH5a......		M1968	cen Gal-Gst Sin4 URA3 Amp
FB1810		D. Stillman		DH5-a.pha.		m1977	"rgr1, TRP1 disruptor
FB1803		D. Stillman		DH5-alpha.		m1979	"rgr1, LEU2 disruptor
FB1813	M1981	D. Stillman		DH5a......		M1981	"lexA, RGR1, His3
FB1809		D. Stillman	see book	MH1.alpha.		m2596	"Rgr1, 2micron, URA3
FB1804		D. Stillman		DH5-alpha.		m2597	"RGR1, URA3, 2micron
FB1816	M2935	D.STILLMAN	HA1-RGR1 integrating construct	DH5a......		M2935	HA1-RGR1 URA3 AMP
FB2391	M598	David Stillman		DH5a......		M598	"HO, URA3, CEN, YCp50
FB2295	MBB81	Mary Bryk	see notebook for map and sequence	DH5a......		MBB81	"sir3D::hisGURA3hisG, pBST KS
FB1578	EBL26	Elion lab	MCM1 in YEp13 See enclosed sheet.	HB101.n...		MCM1	MCM1 ampR 2micron LEU2
FB1579	EBL27	Elion lab	MCM1 in YEp24 See enclosed sheet.	HB101.n...		MCM1	MCM1 ampR 2micron URA3
FB1572	mcm1-1	Tye lab	See enclosed sheet.	unknown...		mcm1-1	mcm1-1 ampR URA3
FB1854	MPY3xHA	Brandt Schneider	vector for HA tagging your favorite gene	DH5a......		MPY3xHA	"URA3, PUCori, ampR
FB1855	MPY3XMYC	Brant Schneider	Vector for MYC tagging your favorite gene	DH5a......		MPY3xMYC	"ampR, URA3, PUCori
FB932	7	Martha Soto		HB101.n...		mutant of pCC11	SPT6 CEN URA3
FB933	6	Martha Soto		HB101.n...		mutant of pCC11	SPT6 CEN URA3
FB934	9	Martha Soto		HB101.n...		mutant of pCC11	SPT6 CEN URA3
FB935	13	Martha Soto		HB101.n...		mutant of pCC11	SPT6 CEN URA3
FB936	15	Martha Soto		HB101.n...		mutant of pCC11	SPT6 CEN URA3
FB937	16	Martha Soto		HB101.n...		mutant of pCC11	SPT6 CEN URA3
FB938	17	Martha Soto		HB101 ....		mutant of pCC11	SPT6 CEN URA3
FB939	18	Martha Soto		HB101.....		mutant of pCC11	SPT6 CEN URA3
FB940	19	Martha Soto		HB101.....		mutant of pCC11	SPT6 CEN URA3
FB941	22	Martha Soto		HB101.....		mutant of pCC11	SPT6 CEN URA3
FB924	205	Martha Soto		HB101.n...		mutant of pDE75-2	ampR TRP1 CEN ARS SPT15
FB925	227	Martha Soto		HB101.n...		mutant of pDE75-2	ampR TRP1 CEN ARS SPT15
FB926	257	Martha Soto		HB101.n...		mutant of pDE75-2	ampR TRP1 CEN ARS SPT15
FB927	264	Martha Soto		HB101.n...		mutant of pDE75-2	ampR TRP1 CEN ARS SPT15
FB928	283	Martha Soto		HB101.n...		mutant of pDE75-2	ampR TRP1 CEN ARS SPT15
FB929	308	Martha Soto		HB101.n...		mutant of pDE75-2	ampR TRP1 CEN ARS SPT15
FB930	311	Martha Soto		HB101.n...		mutant of pDE75-2	ampR TRP1 CEN ARS SPT15
FB931	315	Martha Soto		HB101.n...		mutant of pDE75-2	ampR TRP1 CEN ARS SPT15
FB887		F.W. Studier	see FB885	BL21.(DE3)		none
FB888		F.W. Studier	see FB885	BL21 (DE3)		none
FB889		F.W. Studier	see FB885	HMS174.E3)		none
FB1	DB6507	Botstein	Can screen for URA3 function	..........	pyrF74::Tn5 and all HB101 markers	none
FB4	B28/TR5695	J.R. Roth via G.R. Fink		TR5695....	trp B1 his (P2 eductant)	none
FB18	MC1061	Casadaban	There are two entries of this strain in the FB collection	MC1061....	"araD139 del(ara,leu)7697 dellacX74 galE galK hsr- hsm+ strA	none
FB19	M8820F1	Casadaban		M8820F1...	"araD139 del(ara,leu)7697 del(proABlacIPOZYA)X111 strA ph80 ph80dF1arat rplac	none
FB20	DB6660	Botstein		DB6660....	trp(am) lac(am) pyrF::Mu hsr- hsm+ supF	none
FB115	?	D. Hanahan	high frequency transformer	?.1046....	?	none
FB117	JM101	via Fink	This is strain B234 in the G.R. Fink collection	JM101.....	del(lac-pro) supE thi/ F'traD36 proAB lacIq	none
FB120	ES942	via Colin Manoil		ES942.....	mutD5 metE lacZ trpA F-	none
FB121	LE30	via Colin Manoil	mutD mutagenesis increased by TdR (usually enough in LB)	LE30......	mutD5 rpsL (strR) azi galU95 F-	none
FB173		M. Berman		KK2186....		none
FB281	JM107		streak on M9 glucose + B1 to hold F'	JM107.....	del(lac-pro) thi gyrA96 endA1 hsdR17 relA1 supE44 (F' traD36 proAB lac Iq lacZdelM15)	none
FB332	MC1061	Dan Weeks	good for transformations; also entered earlier in the collection	MC1061....		none
FB383	SE10	Scott Emr	published in an article in JCB	SE10......		none
FB405	BD817	Bruce Duncan	"Gene 28, 211-219 (1984) "	BD817.....	ung1 nadB7 recA56 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 ga lT22 rpsL relA1(?)	none
FB406	BD1528	Bruce Duncan		BD1528....	ung1 nadB30 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 galT22 r psL relA1(?)	none
FB413	NK5012	Nancy Kleckner	strain Q1 from Ethan Signer	NK5012....	C600 TL- B1+ lac- suII T1R isR phi80R	none
FB414	NK7147	Nancy Kleckner	spontaneous lambdaR from NK5360	NK7147....	"gal1,2 su- strA547 lacZ- r-m+ delhis rec+ lambdaR	none
FB415	NK5830	Nancy Kleckner	FB413-FB415 are for lambda1224 (Tn10LUK hops)	NK5830....	F'lacproiQc8/arg dellacproX111 nalA rifR recA su0 ara	none
FB475	CAG10153	Carol Gross	This is a nonsense rpoH allele. Grow the strain at 30 or 35. Ts best seen at 42. See notebook for details.	CAG10153..	MC1061 rpoH165 zhf50::Tn10	none
FB476	CAG1578	Carol Gross	"Ts rpoD allele with linked Tn10. 25-30 is permissive, 42 is nonpermissive. Seenotebook for details. "	CAG1578...	MC1061 rpoD800 Tn10	none
FB507	LE392	Leder lab	originally made by Lynn Enquist	LE392.....	F- hsdR514 (r-K m-K) supE44 supF58 lacY1 or del(lacIZY)6 galK2 galT22 metB1 trpR55 lambda-	none
FB517		K. Struhl		KC8.1.....	leuB600 trpC983 hisB463 pyrF74::Tn5 dellacX74 strA galUK	none
FB520	NK8018	Nancy Kleckner		NK8018....	malB (lambda R) leu2 trp31 his1 argG str64 tonA tsx supE44 xyl7 ntl2 p ro+ lacdelr1 (z-y+) metb	none
FB600	MC1065	"Casadaban, Struhl, Raymond	"For scoring trp, use drop out media. Also see FB326. "	MC1065*...	"X1486 M+h12 leuB600 trpC9830 pyrF::Tn5 hisB463 dellacX74 strA galU,K	none
FB626	JM109		see notebook	JM109.....	recA1 endA1 gyr96 thi hsdR17(rk- mk+) supE44 relA1 lambda- del(lac pro AB)/ F' traD36 proAB lacIqZd	none
FB1505	BMB304	Magasanik Lab	Sau3A partial of yeast DNA cloned into BamHI site of YEp13 REF: MCB vol. 11 :822 (feb 91) Coschigano+Magasanik	HB101.ha..		p1	URE2 ampR LEU2 2micron
FB2202	p101	BCairns		DH5a......		p101	"URA3,CEN,SWP61
FB2203	P102	B Cairns		DH5a......		p102	URA3 CEN SWP59
FB1370	p1107	Roger Brent	derived from pLG670Z (FB1373)	HB101.n...		p1107	LexAop CYC1-TATA-LacZ fusion URA3 ampR 2micron
FB2284	p1229	Chris Brandl	vector pT27	DH5a......		p1229	tra1::hisG-URA3-hisG
FB2217	p123089	B Olsen	pBluescript derivative	?.5a......		p123089	Est clone p123089 into pT7T30
FB1196	p125-3	D. Eisenmann	~6.3kb Xho-Xho piece from original SPT15 clone-- located 3' to the SPT15 gen e and cloned into pRS316	HB101.n...		p125-3	URA3 CEN ARS ampR
FB1206	p1259-1	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 and strain FW1259 into pRS 316	HB101.n...		p1259-1	spt15-21 URA3 CEN ARS ampR
FB850		Dave	gap repair of pDE38-9	HB101.....		p1259-1	spt15-122 pRS316
FB666		Hieter	for transplacement	HB101.....		p13	YRp14 trp1del1
FB2206	p138	b cairns		dh5a......		p138	"URA3,CEN,RSC6
FB2207	p147	b cairns		DH5a......		p147	"URA3,CEN,TFG3
FB2208	p149	b cairns		DH5a......		p149	URA3 CEN SWP73
FB2209	p151	b cairns	TAF130 and RSC14 separated by only 80bp	DH5a......		p151	"URA3,2micron,RSC14 w/TAF130
FB2210	p164	b cairns		DH5a......		p164	"URA3,CEN,RSC4
FB2211	p166	b cairns		DH5a......		p166	"URA3,CEN,RSC1
FB2212	p178	b cairns		DH5a......		p178	URA3 CEN RSC3
FB1449	p180	Hieter	HindIII clone that contains CEN10 in YRp14/ARS (centromere selection vector)	HB101.n...		p180	CEN10 SUP11 URA3 ampR ARS1
FB1450	p181	Hieter	HindIII clone that contains CEN1 in YEp14/ARS (centromere selection vector)	HB101.n...		p181	CEN1 SUP11 URA3 ampR ARS1
FB2515	p1810-NAT	Amine		DH5a		p1810-NAT	replace kanR with NatR
FB2213	p197	b cairns		DH5a......		p197	TRP1 CEN RSC1
FB1506	BMB319	Magasanik Lab	URE2 in YCp50 REF: MCB vol.11:822 (feb91)	HB101.ha..		p1C-CS	URE2 ampR URA3 CEN ARS
FB1507	BMB347	Magasanik Lab	REF: MCB vol.11:822 (feb91)	XL1.1.ha..		p1C-CSDH0	ure2D12::URA3 ampR CEN ARS
FB1451	p200	Hieter	HindIII clone that contains CEN7 in YRp14/ARS (centromere selection vector)	HB101.n...		p200	CEN7 SUP11 URA3 ampR ARS1
FB1452	p201	Hieter	HindIII clone that contains CEN11 in YRp14/ARS (centromere selection vector)	HB101.n...		p201	CEN11 SUP11 URA3 ampR ARS1
FB1175	Struhl#201	Struhl	TATA mut. in this plasmid is AATAAA. REF= MCB dec89 p. 5298-5304 Harbury + Struhl. **SEE NOTEBOOK**	HB101.....		p201	URA3 ampR his3-201 integrating plasmid
FB1176	Struhl#202	Struhl	TATA mut.= CATAAA. REF= MCB dec89 p. 5298-5304 Harbury + Struhl *SEE N OTEBOOK*	HB101.....		p202	URA3 ampR his3-202 integrating plasmid
FB1453	p202	Hieter	HindIII clone containing CEN15 in YRp14/ARS (centromere selection vector)	HB101.n...		p202	CEN15 SUP11 URA3 ampR ARS1
FB1454	p203	Hieter	HindIII clone containing CEN14 in YRp14/ARS (centromere selection vector)	HB101.n...		p203	CEN14 SUP11 URA3 ampR ARS1
FB1177	Struhl#203	Struhl	TATA mut.= GATAAA. REF= MCB dec89 p. 5298-5304 Harbury + Struhl *SEE N OTEBOOK*	HB101.....		p203	URA3 ampR his3-203 integrating plasmid
FB1178	Struhl#204	Struhl	TATA mut.= TCTAAA REF= MCB dec89 p.5298-5304 Harbury + Struhl *SEE NO TEBOOK*	HB101.....		p204	URA3 ampR his3-204 integrating plasmid
FB1179	Struhl#205	Struhl	mutant TATA seq= TGTAAA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p205	ampR URA3 his3-205 integrating plasmid
FB1180	Struhl#206	Struhl	mutant TATA seq= TTTAAA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p206	ampR URA3 his3-206 integrating plasmid
FB1181	Struhl#208	Struhl	mutant TATA seq= TACAAA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p208	ampR URA3 his3-208 integrating plasmid
FB1182	Struhl#209	Struhl	mutant TATA seq= TAGAAA REF: MCB dec89 p. 5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p209	ampR URA3 his3-209 integrating plasmid
FB859		"Parent, Kramer	PHO5 probe (?) see notebook	HB101.....		p20L	amp PHO5 pBR322
FB1183	Struhl#210	Struhl	mutant TATA seq= TATCAA REF: MCB dec89 p. 5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p210	ampR URA3 his3-210 integrating plasmid
FB1184	Struhl#212	Struhl	mutant TATA seq= TATTAA REF: MCB dec89 p.5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p212	ampR URA3 his3-212 integrating plasmid
FB1185	Struhl#213	Struhl	mutant TATA seq= TATACA REF: MCB dec89 p.5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p213	ampR URA3 his3-213 integrating plasmid
FB1186	Struhl#214	Struhl	mutant TATA seq= TATAGA REF: MCB dec89 p.5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p214	ampR URA3 his3-214 integrating plasmid
FB1187	Struhl#215	Struhl	mutant TATA seq= TATATA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p215	ampR URA3 his3-215 integrating plasmid
FB1190	Struhldoub	Struhl	mutant TATA seq= TATATAC REF: MCB dec89 p.5298-5304 Harbury and Struhl * SEE NOTEBOOK!*	HB101.....		p215/238	"ampR URA3 his3-215,238 integrating plasmid
FB1239	Struhl#216	Ricupero .	mutant TATA seq= TATAAC. REF= MCB dec89 p.5298-5304 Harbury and Struhl **S EE ALSO FB1174-1190)	HB101.n...		p216	URA3 ampR integrating plasmid his3-216
FB1188	Struhl#217	Struhl	mutant TATA seq= TATAAG REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p217	ampR URA3 his3-217 integrating plasmid
FB1189	Struhl#218	Struhl	mutant TATA seq= TATAAT REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p218	ampR URA3 his3-218 integrating plasmid
FB2340	p25	Alan Goldstein	"nourseothricin resistance, Yeast 15, 1541-1553 (1999) "	DH5a......		p25	"natMX4, pFA6 vector backbone
FB2341	p29	Alan Goldstein	"Bialaphos resistance, Yeast 15, 1541-1553 (1999) "	DH5a......		p29	"patMX4, pFA6 vector backbone
FB522		Phil Hieter	see notebook; for generating chromosome fragmentation	HB101.....		p299 = YCF3	URA3 SUP11
FB1220	p3-1	D. Eisenmann	spt3-1 rescued from FW240 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-1	spt3-1 URA3 CEN ARS ampR
FB1218	p3-401	D. Eisenmann	original plasmid from gapped rescue using pDE105-6 cut with MluI and XhoI in to spt15-21 revertant strain L805. p3-401 is in pMR79(=YCp50 with XhoI sit e knocked out)	HB101.n...		p3-401	spt3-401 URA3 CEN ARS ampR
FB1223	p3-417	D. Eisenmann	spt3-417 rescued from FY647 into pMR79 (=YCp50 with Xho site knocked out).	HB101.....		p3-417	spt3-417 URA3 CEN ARS ampR
FB1221	p3-426	D. Eisenmann	spt3-426 rescued from FY648 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-426	spt3-426 URA3 CEN ARS ampR
FB1222	p3-429	D. Eisenmann	spt3-429 rescued from FY649 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-429	spt3-429 URA3 CEN ARS ampR
FB1224	p3-441	D. Eisenmann	spt3-441 rescued from FY650 into pMR79 (=YCp50 with Xho site knocked out).	HB101.....		p3-441	spt3-441 URA3 CEN ARS ampR
FB1225	p3-443	D. Eisenmann	spt3-443 rescued from FY651 into pMR79 (=YCp50 with Xho site knocked out).	HB101.....		p3-443	spt3-443 URA3 CEN ARS ampR
FB1219	p3-445	D. Eisenmann	spt3-445 rescued from FY652 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-445	spt3-445 URA3 CEN ARS ampR
FB523		Phil Hieter	see notebook; for chromosome fragmentation	HB101.....		p300 = YCF4	URA3 SUP11
FB667		Hieter	for leu2 transplacement; see notebook	.B101.....		p307	YRp15 leu2del1
FB2342	p32	Alan Goldstein	"Hygromycin B resistance, Yeast 15, 1541-1553 (1999) "	DH5a......		p32	"hphMX4, pFA6 vector backbone
FB2512	pKS-ura4	DH5a		DH5a		p358	pBluescript Sp. ura4
FB1004	35BK	Patty Minehart	"GLN3 disrupted w/ LEU2 (same as in FB1003). AatII frag with Bam linkers on each end, then subcloned into Bam of KS+ plasmid (Bluescript). "	HB101.....		p35BK	LEU2 ampR ori
FB1795		Dave Rose .	genmic clone from Hieter library that contains CHD1 and PAB1	MH1		p36-1	"CEN, ARS, LEU2, AmpR, CHD1, PAB1
FB2443	L90	Doug Koshland	"18S, 5.8S and part of 25S BglII fragment see notebook "	HB101.....		p362	rDNA FISH probe
FB2444	L92	Doug Koshland	"25S and part of 5S BglII fragment, see notebook "	HB101.....		p363	rDNA fish probe
FB1445	p37/7	C. Connelly	see enclosed sheets	unknown...		p37/7	ctf4d3::URA3 ampR LEU2 CEN ARS
FB2147	p39	M.Snyder		DH5a......		p39	KNS1
FB1534	p413-GAL-L	Hieter Lab	See sheets in notebook!	DH5alpha..		p413-GAL-L	GAL-L promoter CYC1 term HIS3 ampR CEN ARS
FB1542	p413-GAL-S	Hieter Lab	See sheets in notebook!	DH5alpha..		p413-GAL-S	GAL-S promoter CYC1 term HIS3 ampR CEN ARS
FB1526	p413-GAL1	Hieter lab	See sheets in notebook!	DH5alpha..		p413-GAL1	GAL1 promoter CYC1 term HIS3 ampR CEN ARS
FB1518	p413-MET25	Hieter lab	See sheets in notebook!	DH5alpha..		p413-MET25	MET25 promoter CYC1 term HIS3 ampR CEN ARS
FB1535	p414-GAL-L	Hieter Lab	See sheets in notebook!	DH5alpha..		p414-GAL-L	GAL-L promoter CYC1 term TRP1 ampR CEN ARS
FB1543	p414-GAL-S	Hieter Lab	See sheets in notebook!	DH5alpha..		p414-GAL-S	GAL-S promoter CYC1 term TRP1 ampR CEN ARS
FB1527	p414-GAL1	Hieter lab	See sheets in notebook!	DH5alpha..		p414-GAL1	GAL1 promoter CYC1 term TRP1 ampR CEN ARS
FB1519	p414-MET25	Hieter lab	See sheets in notebook!	DH5alpha..		p414-MET25	MET25 promoter CYC1 term TRP1 ampR CEN ARS
FB1536	p415-GAL-L	Hieter Lab	See sheets in notebook!	DH5alpha..		p415-GAL-L	GAL-L promoter CYC1 term LEU2 ampR CEN ARS
FB1544	p415-GAL-S	Hieter Lab	See sheets in notebook!	DH5alpha..		p415-GAL-S	GAL-S promoter CYC1 term LEU2 ampR CEN ARS
FB1528	p415-GAL1	Hieter Lab	See sheets in notebook!	DH5alpha..		p415-GAL1	GAL1 promoter CYC1 term LEU2 ampR CEN ARS
FB1520	p415-MET25	Hieter lab	See sheets in notebook!	DH5alpha..		p415-MET25	MET25 promoter CYC1 term LEU2 ampR CEN ARS
FB1537	p416-GAL-L	Hieter Lab	See sheets in notebook!	DH5alpha..		p416-GAL-L	GAL-L promoter CYC1 term URA3 ampR CEN ARS
FB1545	p416-GAL-S	Hieter Lab	See sheets in notebook!	DH5alpha..		p416-GAL-S	GAL-S promoter CYC1 term URA3 ampR CEN ARS
FB1529	p416-GAL1	Hieter Lab	See sheets in notebook!	DH5alpha..		p416-GAL1	GAL1 promoter CYC1 term URA3 ampR CEN ARS
FB2436	p416GAL1	A. Aguilera		DH5a......		p416-GAL1-lacZ	pGAL1-lacZ URA3 CEN
FB1521	p416-MET25	Hieter lab	See sheets in notebook!	DH5alpha..		p416-MET25	MET25 promoter CYC1 term URA3 ampR CEN ARS
FB1147	p419	Reed via Elion	YEp13 with a 3kb EcoRI frag containing most of CLN1 with a TRP1 insertion	unknown...		p419	cln1::TRP1 (weak trp+) LEU2 2micron ampR
FB1538	p423-GAL-L	Hieter Lab	See sheets in notebook!	DH5alpha..		p423-GAL-L	GAL-L promoter CYC1 term HIS3 2micron ampR
FB1546	p423-GAL-S	Hieter Lab	See sheets in notebook!	DH5alpha..		p423-GAL-S	GAL-S promoter CYC1 term HIS3 2micron ampR
FB1530	p423-GAL1	Hieter Lab	See sheets in notebook!	DH5alpha..		p423-GAL1	GAL1 promoter CYC1 term HIS3 2micron ampR
FB1522	p423-MET25	Hieter lab	See sheets in notebook!	DH5alpha..		p423-MET25	MET25 promoter CYC1 term HIS3 2micron ampR
FB1539	p424-GAL-L	Hieter Lab	See sheets in notebook!	DH5alpha..		p424-GAL-L	GAL-L promoter CYC1 term TRP1 2micron ampR
FB1547	p424-GAL-S	Hieter Lab	See sheets in notebook!	DH5alpha..		p424-GAL-S	GAL-S promoter CYC1 term TRP1 2micron ampR
FB1531	p424-GAL1	Hieter Lab	See sheets in notebook!	DH5alpha..		p424-GAL1	GAL1 promoter CYC1 term TRP1 2micron ampR
FB1523	p424-MET25	Hieter lab	See sheets in notebook!	DH5alpha..		p424-MET25	MET25 promoter CYC1 term TRP1 2micron ampR
FB1540	p425-GAL-L	Hieter Lab	See sheets in notebook!	DH5alpha..		p425-GAL-L	GAL-L promoter CYC1 term LEU2 2micron ampR
FB1548	p425-GAL-S	Hieter Lab	See sheets in notebook!	DH5alpha..		p425-GAL-S	GAL-S promoter CYC1 term LEU2 2micron ampR
FB1532	p425-GAL1	Hieter Lab	See sheets in notebook!	DH5alpha..		p425-GAL1	GAL1 promoter CYC1 term LEU2 2micron ampR
FB1524	p425-MET25	Hieter lab	See sheets in notebook!	DH5alpha..		p425-MET25	MET25 promoter CYC1 term LEU2 2micron ampR
FB1541	p426-GAL-L	Hieter Lab	See sheets in notebook!	DH5alpha..		p426-GAL-L	GAL-L promoter CYC1 term URA3 2micron ampR
FB1549	p426-GAL-S	Hieter Lab	See sheets in notebook!	DH5alpha..		p426-GAL-S	GAL-S promoter CYC1 term URA3 2micron ampR
FB1533	p426-GAL1	Hieter Lab	See sheets in notebook!	DH5alpha..		p426-GAL1	GAL1 promoter CYC1 term URA3 2micron ampR
FB1525	p426-MET25	Hieter lab	See enclosed sheets in notebook!	DH5alpha..		p426-MET25	MET25 promoter CYC1 term URA3 2micron ampR
FB668		Hieter	for trp1 transplacement; see notebook	XL1-13....		p430	YRp14 trp1del63
FB2576	p433	Angelica Amon	AF mutation introduces a BsmI site in cdc28			p433	cdc28-T18A Y19F URA3
FB2141	p49	M.Snyder		DH5a.DE3..		p49	KNS1
FB2711	p4E-GST	Curt Hagedorn	PCR product of the human cap binding protein gene eIF4EK119A (aa residues 28-217) cloned into the Bam HI site of pGEX-4T-3	DH5a		p4E-GST	AmpR
FB2711		Curt Hagedorn	pGEX-4T-3 backbone	DH5a		p4E-GST	AmpR
FB669		M. Fasullo via P. Hieter	for his3 transplacement	RR1.13....		p510	YRp14 his3del200
FB2142	p54	M.Snyder		DH5a.DE3..		p54	KNS1
FB2143	p55	M.Snyder		DH5a.DE3..		p55	"KNS1,p55
FB2249	p5921	William Fonzi	for making C.albicans deletions	DH5a......		p5921	"pUC18, hisG-URA3-hisG
FB1852	p6/50	Stan Field via Elaine Elion		?.........		P6/50	"ampR, URA3, CEN, STE12
FB2176	p64 pYK116	Giora Simchen		DH5a......		p64 pYK116	"yEp24, CDC40
FB158	CY37	Kurjan & Herskowitz	Sau3A partial into YEp13; contains MFalpha1 (alpha factor)	MH1.3.....	araD139 lac-delX74 galU galK hsr hsm+ strA	p69A	YEp13 MFalpha1 (alpha factor)
FB920		Christine Chapon	"SPT8 Bam-Bam in pBM272, same as p7.4 in FB919 "	unknown...		p7.14(757-14)	SPT8
FB922		Christine Chapon	"SPT8 Bam-Bam in pBM272, same as p7.5 in FB921 "	unknown...		p7.15(757-15)	SPT8
FB919		Christine Chapon	SPT8 Bam-Bam in pBM272	unknown...		p7.4(757-4)	SPT8
FB921		Christine Chapon	"SPT8 Bam-Bam in pBM272, opposite orientation from p7.4 in FB919 "	unknown...		p7.5(757-5)	SPT8
FB2414	p75	Nick Proudfoot	ref EMBO V.17 No.16 p.4771-4779	DH5a......		p75	GAL10 3'UTR deletion CEN TRP1
FB1267	771	Roger Brent	EcoRV-RsaI frag containing cyc1-512 into StuI site of pRB486 (see enclosed s heet)	HB101.n...		p771	cyc1-512 terminator ampR
FB849		Dave	spt15-135 rescued by gap repair	HB101.....		p771-5	pRS316 spt15-135 Cla-Sac
FB2129	p77B1	J.Madison		DH5a......		p77B1	"URA3,CEN,mutant TBP in pRS316
FB1268	798	Roger Brent	EcoRV-RsaI containing CYC1 into StuI site of pRB486 (see enclosed sheet).	HB101.n...		p798	CYC1 terminator ampR
FB1146	p810	Reed via Elion	YRp7 with a HindIII-SalI frag (4kb) containing the rest of CLN2 disrupted by LEU2	unknown...		p810	cln2::LEU2 ampR TRP1 ARS1
FB1204	p969-1	D. Eisenmann	original gap rescued plasmid containing spt15-122 in pRS316. pDE38-9 was us ed to rescue this allele from FW969.	HB101.n...		p969-1	spt15-122 URA3 CEN ARS ampR
FB848		Dave		HB101.ha..		p969-1	spt15-122 on SPT15 Cla-Sac in pRS316
FB1205	p971-5	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 from strain FW971 into pRS 316	HB101.n...		p971-5	spt15-135 URA3 CEN ARS ampR
FB1962	pA3P2L	Guarente Lab		DH5a......		pA3P2L	"CEN, LEU2, ADA3
FB632		Antebe and Fink	"J. Bact. 166, 313 (1986); subclone of pTLG2 into YIp5; RI-H3 fragment "	HB101.....		pAA1	YIp5 GAL2
FB2553	pAAD	K.Takegawa	Yeast 2005;22:193-202	Dh5a		pAAD	ade7+ pombe ARS
FB2423	pAAD11	Andrea Duina		DH5a......		pAAD11	hht2-11-HHF2 TRP1 CEN
FB2431	pAAD180	Andrea Duina		MH1.......		pAAD180	hht2-11-HHF2 TRP1 2micron
FB2420	pAAD3	Andrea Duina		DH5a......		pAAD3	"hht2-3-HHF2 TRP1, CEN
FB2424	pAAD41	Andrea Duina		DH5a......		pAAD41	hht2-41-HHF2 TRP1 CEN
FB2425	pAAD44	Andrea Duina		DH5a......		pAAD44	hht2-44-HHF2 TRP1 CEN
FB2426	pAAD48	Andrea Duina		DH5a......		pAAD48	hht2-48-HHF2 TRP1 CEN
FB2427	pAAD49	Andrea Duina		DH5a......		pAAD49	hht2-48-HHF2 TRP1 CEN
FB2421	pAAD5	Andrea Duina		DH5a......		pAAD5	hht2-5-HHF2 TRP1 CEN
FB2428	pAAD51	Andrea Duina		DH5a......		pAAD51	hht2-51-HHF2 TRP1 CEN
FB2429	pAAD56	Andrea Duina		DH5a		pAAD56	hht2-56-HHF2 TRP1 CEN
FB2430	pAAD57	Andrea Duina		DH5a......		pAAD57	hht2-57-HHF2 TRP1 CEN
FB2422	pAAD7	Andrea Duina		DH5a......		pAAD7	hht2-7-HHF2 TRP1 CEN
FB1369	pAAH5	Roger Brent		HB101.n...		pAAH5	PADH1vector 2micron LEU2 ampR
FB1169	pAB1	A. Bortvin	3.5kb SPT5 frag from pMS4 cloned into SmaI site of pJK1521. LexA-SPT5 fusio n= 12-1064 a.a.	HB101.....	sick	pAB1	SPT5-lexA TRP1 CEN ARS ampR
FB1774		Alex	A derivative of pAB148 looking ARS CEN sequences integrate via BstEII in LEU 2	unknown...		pAB157	LEU2 Amp
FB1775			pAB157 w/o HIII msc. fragment	?.........		pAB203	LEU2 Amp (H3d.H4)d16'
FB1776	pAB204		pAb157 w/o BspI-RI fragment both BspI site and RI site still there	..........		pAB204	LEU2 Amp (H3-H4d)d16'
FB1777	pAB221	Alex.	pAB157 w/o aa 4-30 in H3	.nknown...		pAB221	LEU2 Amp (H3d4.30-H4)
FB1778	pAB224	Alex.	pAB157 w/PCR fragment from FY869 carrying bur5-1 mutation	.nknown...		pAB224	LEU2 Amp.
FB1286	pAB24	Chiron Corp. (T. Brake)	*K. lactis library* REF: NAR vol.19 No.19: 5345-5350. See Yeast Library notebook	DH5.own...		pAB24	K. lactis library URA3 ampR
FB77		B. Errede		HB101.....		pAB25	CYC7
FB533	CY786	George Sprague		RR1.1.....		pAB510	pBR322 STE2
FB1168	pAB56	A. Bortvin	BglII spt6-140 frag cloned into BamHI site of YCp50 that has BglII-SmaI frag (CEN ARS) deleted. see also FB1167.	HB101.....		pAB56	spt6-140 URA3 ampR
FB1170	pAB57	A. Bortvin	same as FB1169 but fusion of lexA with SPT5 starting at a.a.#632-1064.	HB101.....	sick	pAB57	SPT5-lexA TRP1 CEN ARS ampR
FB527		Dave Hill	do not give out	HB101.....		pAB701	GPDH vector
FB1826	pAB71	Alex Bortvin		HB101.....		pAB71	"CEN, ARS1, LEU2, DED1::GCN4
FB860		Anne Chiang	SPT9 candidate E from YCp50 library	HB101.....		pAC1	SPT9 URA3 amp
FB861		Anne Chiang	no SPT9 function; see notebook	TB1.1.....		pAC10	"SPT9 subclone, Bam deletion
FB862		Anne Chiang	probably not functional	TB1.1.....		pAC12	pRS306 SPT9 Bam-Sal
FB863		Anne Chiang	partial SPT9 function	TB1.1.....		pAC14	SPT9 Mlu deletion clone in YCp50
FB1938	pAC153	Marian Carlson's lab	ref. Abrams et al. MCB (1986) 6(11) 3643-3651	..1.......		pAC153	"2micron, URA3, SNF5, amp
FB864		Anne Chiang	no SPT9 function	.B1.1.....		pAC16	YCp50 SPT9 HindIII deletion clone
FB865		Anne Chiang	no SPT9 function	.B1.1.....		pAC18	YCp50 SPT9 BglII clone
FB715		M. Carlson	"for transplacement of snf5 null; MCB 6, 3647 "	HB101.....		pAC186	amp snf5::URA3
FB866		Anne Chiang	probably non-functional; should be rechecked	TB1.1.....		pAC20	pRS316 SPT9 region Bam fragment
FB867		Anne Chiang	not tested for function	.B1.1.....		pAC22	"SPT9, SacI piece reclosed
FB714		M. Carlson	"MCB 6, 3647; for snf2 transplacements "	HB101.....		pAC98	amp snf2::HIS3
FB1299	pACR1	Roger Brent		DH5 (?)..		pACR1	ACR1 ampR URA3
FB2257	pACTII	C Brandl	2 hybrid	MH1.......		pACTII	"TRP1, 2 micron
FB1487	pAD1	A. Dudley	1.5kb SacI-ClaI frag from pLG39 (FB1350) containing his4-912dD44-lacZ subclo ned into 4.2kb SacI/ClaI of pRS304	HB101.3...		pAD1	TRP1 ampR his4-912dD44-lacZ
FB1983	pAD1-23-6			DH5a......		pAD1-23-6	"TRP1, AmpR, his4-912dD44-lacZ(23)
FB1984	pAD1-26-8			DH5a......		pAD1-26-8	"TRP1, AmpR, his4-912dD44-lacZ(26)
FB1488	pAD11	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid sucloned into PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific mutati on seq.	HB101.3...		pAD11	his4-912dD44-lacZ-pAD11 TRP1 ampR
FB1489	pAD12	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487) See enclosed sheets for specific muta nt seq.	HB101.3...		pAD12	his4-192dD44-lacZ-pAD12 TRP1 ampR
FB1490	pAD13	A. Dudley	PacI-BseRI frag (320bp) from site directed mutagenesis plasmid subcloned int o PacI-BseRI sites of pAD1 (FB1487). See enclosed sheets for specific mut ant seq.	HB101.3...		pAD13	his4-912dD44-lacZ-pAD13 TRP1 ampR