Bacteria

Displaying 1 - 500 of 2807

Strain Name	Strain Alias	Source	Comments	Host	Host Markers	Plasmids	Plasmid Markers Sort descending
						pGH127
						pGH%
FB2456	pET-15b	Amine		DH5a		pET-15b
FB
FB2461	4N	Bob Kingston	for assembling a tetra-nucleosome array	?		4N
FB2460	3N	Bob Kingston	for assembling a tri-nucleosome array	?		3N
FB2459	2N	Bob Kingston	for assembling a di-nucleosome array	?		2N
FB2458	1N	Bob Kingston	For assembling a mono-nucleosome	?		1N
						pFW217
						pRM200
						pRM%

		Joel

FB2491	pGN965					pGN965
FB2492	BL21/DE3	Rolf Sternglanz	Super codon plus strain for protein expression	BL21/DE3	Chloramphenicol
						pNOY436
				fb2195
						phz1
	pms4
pms4
FB2516



						pMS37
						psh17-4
FB2114
%FB2114%

fb2044
	dh5a
	dh5alpha
fb843
fb843
	dh5a

						pLBS27
						pLBS27
						pMRFW2
fb603
FB

FB
fb
FB2702	pLP891				SIR2 HIS3	pLP891
FB2703	pLP1047				SIR3 HIS3	pLP1047
FB2704	pLP2206				SIR4 HIS3	pLP2206
FB2705	pPK128				HTA1-HTB1 HHT1-HHF1 LEU2	pPK128
FB2706				DH5a	SPO74-GFP	pRS314 SPO74-GFP
FB2707				DH5a	SPO21-GFP	pRS316 SPO21-GFP
FB2710	pCM225	Euroscarf		DH5a	AmpR KanR	pCM225

FB2714	JF340116	Anthony Carr	Watson et al. 2011, Gene 484 pp 75-85 Derived from pAW8 from Watson et al. 2008, Gene 407, pp 63-74	DH5a	AmpR	pAW8 ENdeI-cyEGFP
FB2715	JF340118	Anthony Carr	Watson et al. 2011, Gene 484 pp 75-85 Derived from pAW8 from Watson et al. 2008, Gene 407, pp 63-74	DH5a	AmpR	pAW8 ENdeI-C3HA
FB2716	JF340115	Anthony Carr	Watson et al. 2011, Gene 484 pp 75-85 Derived from pAW8 from Watson et al. 2008, Gene 407, pp 63-74	DH5a	AmpR	pAW8 ENdeI
FB2730	Stbl3	Invitrogen	Life Technologies Corporation Catalog #C737303 Lot# 1649448	Stbl3
FB2750		WeissmanLab/Stirling Churchman	for making ligase for NetSeq. Protein runs around 30 kDA	DH5a	ampR	pT4RNL2(1-249) K227Q
FB2751	TG11		Stored as plasmid DNA, not in bacteria.	None		pTAL08
FB2753	pGZ110	Rolf Sternglanz	For crispr-mediated mutagenesis	DH5a	ampR	pRS425-Cas9-2xSapI
FB2754	pGZ110-Kan	Rolf Sternglanz	For crispr-mediated mutagenesis	DH5a	ampR	pRS425-Cas9-kanMX
FB2806	pMAX175	Boeke Lab	Superloser		kanR URA3 URA3syn CEN ARS	pMAX175
FB2843	pKW26			DH5alpha	PRS414 + z3evprom-RNH1(wt)-3xV5-adh1term	pKW26
FB2844	pRS416		Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (1/4)			pRS416
FB2845	pRS416		Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (2/4)			pRS416
FB2846	pRS416		Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (3/4)			pRS416
RB2847	pRS416		Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (4/4)			pRS416
FB1439	GM48	G. Duyk	contains NO dam or dcm methylases (from NEB catalogue)	GM48	F- thr leu thi lacY galK ara fhuA tsx dam dcm SUPE44
FB1440				.B1.1.....		pT2
FB1441				.B1.1.....		pT1-3B
FB1442				.B1.1.....		pIP5469
FB1585	JM107----	S. Elledge	Full name of HOST= JM107/lambdaKC (is the host strain used for Steve Elledg e lambda library (10X/cre sys) See enclosed sheets.	JM107/lKC.	KanR lambda lysogen cre lacI
FB1614		Golderg .	good host for expressing foreign proteins in bact.	LC137.n...	htpR
FB1615		Golberg .	Good host for expressing foreign proteins in bact.	Clp-	Clp-
FB1645	K802	Grant Hartzog		K802......	F- e14- (McrA-) lacY1 or d(lac)6 supE44 galK2 galT22 rfbD1 metB1 mcrB1 hsdR2 (r-k M+k)
FB1659	PLKF'	Y. Kohara .	"for M13 rescue of lamda zap, general M13 work. See enclosed sheet. "	PLKF'	"e14-(mcrA) mcrB1 lac recA hsdR2 (rK-,mK-) supE44 galK2 galT22 metB1 F' proAB laclqZDM15 TN10(tetR
FB1688	C600	T. Lane (Leder lab)	original C600 isolate was EcoK r+m+ McrBC+ See also BNN93 with enclosed s heet in notebook	C600......	F- e14- (McrA-) thr-1 leuB6 thi-1 lacY1 supE44 rfbD1 fhuA21 EcoK r+m+ McrBC+
FB1986	pDR14	Dave Rose		.H5a......
FB1140		A. Rowley + Curt Wittenburg		HB101.....
FB1172	MH1	Lorraine Marsh	*Good for transformation with Smash and Grab DNA* see enclosed sheet in notebook for transformation procedure.	MH1.1.....
FB1260	RZ1032	B. Berg	for dut ung mutagenesis	RZ1032....	dut1 ung1 thi1 relA HfrKL16 PO/45 [lysA61-62] 2bd-279::Th10 supE44
FB1	DB6507	Botstein	Can screen for URA3 function	..........	pyrF74::Tn5 and all HB101 markers	none
FB4	B28/TR5695	J.R. Roth via G.R. Fink		TR5695....	trp B1 his (P2 eductant)	none
FB10	SF1032	Froshauer	MC4100 genetic background	SF1032....	srl::Tn10 reca ara relA lac(del) strR
FB18	MC1061	Casadaban	There are two entries of this strain in the FB collection	MC1061....	"araD139 del(ara,leu)7697 dellacX74 galE galK hsr- hsm+ strA	none
FB19	M8820F1	Casadaban		M8820F1...	"araD139 del(ara,leu)7697 del(proABlacIPOZYA)X111 strA ph80 ph80dF1arat rplac	none
FB20	DB6660	Botstein		DB6660....	trp(am) lac(am) pyrF::Mu hsr- hsm+ supF	none
FB32	PNN49			LE392.....	met r- m+ SupE SupF	YRp14
FB33	PNN50			LE392.....	met r- m+ SupE SupF	YRp15
FB38			pAJ50 entered elsewhere	JM101.....	(lac pro)del supE B1/ F' traD36 proAB LacIq lac7M15	pAJ50
FB81	LG90	Guarante	"Guarante and Ptashne PNAS 78,2199-2203 "	LG90.7....	F- dellacproXIII
FB90	B6	via Fink		?.6507....		pBR322
FB101	B524	Weinstock et al	"see Weinstock et al, PNAS 80, 4432, 4436 "	MH1000....	ompB101	pORF1
FB111	B638	D. Schild via Fink	see Current Genetics 7:85-92	JA300.....	see FB108	pSM13
FB112	HB101	via Fink	B324 in GR Fink collection	.A300.....	thr leu pro B1 supE44 r- m- recA
FB113	B522	Silhavy	"PNAS 80,4432 "	MH3000....	"araD139 del(ara,leu)7697 del(lac)X74 galU galK rpsL (strR) ompR101
FB114	B523	Silhavy		TK1046....	araD139 del(argF-lac)U169 rpsL150(strR) relA1 flbB5301 deoC1 ptsF25 ma lPQ::Tn5 ompBcs1
FB115	?	D. Hanahan	high frequency transformer	?.1046....	?	none
FB116	K38			K38.46....	HfrC
FB117	JM101	via Fink	This is strain B234 in the G.R. Fink collection	JM101.....	del(lac-pro) supE thi/ F'traD36 proAB lacIq	none
FB120	ES942	via Colin Manoil		ES942.....	mutD5 metE lacZ trpA F-	none
FB121	LE30	via Colin Manoil	mutD mutagenesis increased by TdR (usually enough in LB)	LE30......	mutD5 rpsL (strR) azi galU95 F-	none
FB157	JM103		"Find markers in most catalogs, eg NE Biolabs, BRL; M13 host "	JM103.....		F'pro
FB171	KK2186	M. Berman	single stranded vector	KK2186....		pZ150
FB172		M. Berman	single stranded vector	KK2186....		pZ152
FB173		M. Berman		KK2186....		none
FB186			deleted	.B101.....
FB236	RR1	T.J. Koerner and A. Tzagaloff	Other markers not known; probably rec+. Host for pATH vectors for trpE fusi ons.	RR1.07....	r- m-
FB237	RR1delM15	Lothar	host for pUR290-2 plasmids	RR1delM15.	r- m- lacZalpha deletion
FB274	MM572	Martin Marinus	"dam, to make DNA cuttable by certain enzymes such as BclI "	GM237.....	dam-3 recA1 sin-1 thr-1 leuB6 proA2 metB1 thi1 deoB16 ara-14 lacY1 gal K2 rspL260 tsx33 supE44 mut4
FB281	JM107		streak on M9 glucose + B1 to hold F'	JM107.....	del(lac-pro) thi gyrA96 endA1 hsdR17 relA1 supE44 (F' traD36 proAB lac Iq lacZdelM15)	none
FB285	TB1	BRL	For transformaing pUCclones into r-m+	TB1.1.....	ara del(lac pro) thi strA phi80dlacZ delM15 r-K m+K
FB286		Heffron	see notebook	RDP146....		pOX38::m-Tn3(lacZ)
FB287		Heffron	see notebook	RDP146....		pOX38::m-Tn3(URA3)
FB288		Heffron	see notebook	RDP146....		pOX38::m-Tn3(TRP1)
FB289		Heffron	see notebook	RDP146....		pOX38::m-Tn3(HIS3)
FB290		Heffron	see notebook	RDP146....		pOX38::m-Tn3(LEU2)
FB291		Heffron	see notebook	RDP146....		pOX38::m-Tn3
FB292		Heffron	see notebook	RDP146....		pOX38::M-Tn3leu2-lac
FB293		Heffron	see notebook	RDP146....		pOX38::m-Tn3his3-lac
FB294		Heffron	see notebook	RDP146....		pOX38::m-Tn3ura3-lac
FB295		Heffron	see notebook	RDP146....		pOX38::m-Tn3trp1-lac
FB296		Heffron	see notebook	RDP146....		pLB101
FB297		Heffron	see notebook	RDP145....		pHSS6
FB298		Heffron	see notebook	NS2114....
FB309		Promega	see notebook	HB101.....		pGEM1
FB310		Promega	see notebook	HB101.....		pGEM2
FB326	MC1065	M. Casadaban	can use to check yeast TRP1	MC1065....	F- galE15 galK16 dellacX74 (pro+) leuB6 trpC9830 strA(rspL) B1-
FB332	MC1061	Dan Weeks	good for transformations; also entered earlier in the collection	MC1061....		none
FB351	LM1035	Connie Cepko	derivative of HB101; supposed good transforming strain	LM1035....	same as HB101
FB352	AR68	SmithKline	good for production of hybrid proteins; see notebook	AR68.5....
FB843	DH5alpha	Bob Moreland	good transformation strain	DH5alpha..	see notebook
FB885		F.W. Studier	FB885-894 are for expression via T7 RNA polymerse. See Fred or Greg for the article describing all of these vectors and strains.	BL21 (DE3)		pLysS
FB886		F.W. Studier	see FB885	HB21 (DE3)		pLysE
FB887		F.W. Studier	see FB885	BL21.(DE3)		none
FB888		F.W. Studier	see FB885	BL21 (DE3)		none
FB889		F.W. Studier	see FB885	HMS174.E3)		none
FB890		F.W. Studier	see FB885	HMS174.E3)		pET3
FB891		F.W. Studier	see FB885	HMS174.E3)		pET-39
FB892		F.W. Studier	see FB885	HMS174.E3)		pET-36
FB893		F.W. Studier	see FB885	HMS174.E3)		pET-8c
FB894		F.W. Studier	see FB885	HMS174.E3)		pET-3c
FB910		Tricia Davis		.B1 ..n...
FB911		Tricia Davis		AB1886	F- uvrA6 thr1 leu6 thi1 lacV1 galkz ara14 xyl5
FB923		Dave Deitcher		TG1.own...	"JM101, Ecok
FB1323	DH10/P3	Grant Hartzog	E. coli strain for propagating supF plasmids	DH10/P3...	"F- mcrA d(mrr-hsd RMS-mcrBC) 0 80d lacZdM15 dlacX74 deoR recA1 arad139 a(ara, leu)7697 galu galk
FB1359	XL1-Blue	Stratagene	can use for M13 helper phage preps. See enclosed sheets in notebook.	XL1-Blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F` proAB lacIq ZdM15 tn10 tet-]
FB2181		David Stillman	"resistant to killing by DNA gyrase, for use with pRS416-0 "	NH975.....
FB2182		Beckwith Lab		DHB4......
FB2196	pRSETb	Grace Gill		DH5a......		pRSETb
FB2197	pRSETc	Grace Gill		DH5a......		pRSETc
FB2198	pGEX4T-1	Grace Gill	from Pharmacia	DH5a......		pGEX4T-1
FB2199	pGEX4T-3	Grace Gill	from Pharmacia	DH5a......		pGEX4T-3
FB2200	pGEX5X-1	Grace Gill	from Pharmacia	DH5a......		pGEX5X-1
FB2201	pRSETa	GRace Gill		DH5a......		pRSETa
FB2214	pRSQ2-URA3	Snyder lab	for use with the Tn3-LEU2-LacZ library	?.5a......		pRSQ2-URA3
xit
		rothstein
FB383	SE10	Scott Emr	published in an article in JCB	SE10......		none
FB405	BD817	Bruce Duncan	"Gene 28, 211-219 (1984) "	BD817.....	ung1 nadB7 recA56 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 ga lT22 rpsL relA1(?)	none
FB406	BD1528	Bruce Duncan		BD1528....	ung1 nadB30 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 galT22 r psL relA1(?)	none
FB413	NK5012	Nancy Kleckner	strain Q1 from Ethan Signer	NK5012....	C600 TL- B1+ lac- suII T1R isR phi80R	none
FB414	NK7147	Nancy Kleckner	spontaneous lambdaR from NK5360	NK7147....	"gal1,2 su- strA547 lacZ- r-m+ delhis rec+ lambdaR	none
FB415	NK5830	Nancy Kleckner	FB413-FB415 are for lambda1224 (Tn10LUK hops)	NK5830....	F'lacproiQc8/arg dellacproX111 nalA rifR recA su0 ara	none
FB432	NK5772	Nancy Kleckner	See notebook about checking markers by 2-aminopurine test.	NK5772....	dcm-6 dam-3 metB1 galK2 galT22 lacY1 tsx-78 (thi-1 tonA32 mtl-1)? prob ably an amber suppressor str
FB439			DO NOT USE	.B101.....
FB440			DO NOT USE	.B101.....
FB474		Kate		FB415.....		pNK629 pFW120
FB475	CAG10153	Carol Gross	This is a nonsense rpoH allele. Grow the strain at 30 or 35. Ts best seen at 42. See notebook for details.	CAG10153..	MC1061 rpoH165 zhf50::Tn10	none
FB476	CAG1578	Carol Gross	"Ts rpoD allele with linked Tn10. 25-30 is permissive, 42 is nonpermissive. Seenotebook for details. "	CAG1578...	MC1061 rpoD800 Tn10	none
FB485				.B101.....
FB507	LE392	Leder lab	originally made by Lynn Enquist	LE392.....	F- hsdR514 (r-K m-K) supE44 supF58 lacY1 or del(lacIZY)6 galK2 galT22 metB1 trpR55 lambda-	none
FB517		K. Struhl		KC8.1.....	leuB600 trpC983 hisB463 pyrF74::Tn5 dellacX74 strA galUK	none
FB520	NK8018	Nancy Kleckner		NK8018....	malB (lambda R) leu2 trp31 his1 argG str64 tonA tsx supE44 xyl7 ntl2 p ro+ lacdelr1 (z-y+) metb	none
FB552		Jan		HB101.....		pJF74
FB553		Jan		HB101.....		pJF75
FB555		Jan		MC1061....		pJF178
FB556		Jan Fassler		MC1061....		pJF179
FB557		Jan Fassler		.C1061....		pJF182
FB558		Jan Fassler		MC1061....		pJF195
FB559		Jan Fassler		MC1061....		pJF203
FB600	MC1065	"Casadaban, Struhl, Raymond	"For scoring trp, use drop out media. Also see FB326. "	MC1065*...	"X1486 M+h12 leuB600 trpC9830 pyrF::Tn5 hisB463 dellacX74 strA galU,K	none
FB626	JM109		see notebook	JM109.....	recA1 endA1 gyr96 thi hsdR17(rk- mk+) supE44 relA1 lambda- del(lac pro AB)/ F' traD36 proAB lacIqZd	none
FB688		Kate	for Tn10LUK hops into SPT10	FB413.....		pNK629 pFW217
FB689		Kate	for Tn10LUK hops into SPT15	FB413.....		pNK629 pFW219
FB692		Kate		FB413.....		pNK629 pFW213
FB735		B. Daignan-Fornier	This strain is for making MuLEU2 hops; see notebook	JM109.....	JM109 MuCts ZZ4 CAMR
FB736		B. Daignon-Fornier	see notebook	M8820.....
FB774				.B101.....		pGC21
FB782				.B101.....
FB2322	13Myc-His3	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-13Myc-His3MX6	"13Myc, ADH1term, His3MX6
FB2320	13Myc-kan	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-13Myc-kanMX6	"13Myc, ADH1term, kanMX6
FB2321	13Myc-TRP1	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-13Myc-TRP1	"13Myc, ADH1term, TRP1
FB1939	pEY111	Marian Carlson lab	"ref. Estruch & Carlson, MCB 1986 10(6) 2544-2553 "	..1.......		pEY111	"2 micron URA3, SNF6
FB906		Maynard Olsen	"Gene 27, 239 "	unknown...		pPM256	"2 micron, LEU2, SUP53-a
FB2011	jmb3	j madison	see notebook	dh5a......		pJM3	"2 micron, URA3, KlSPT3
FB2012	JMB4	J Madison	see notebook	DH5a......		pJM4	"2 micron, URA3, KlSPT3
FB2084		Berger Lab		DH5a......		G/V	"2micron Gal4-VP16, LEU2
FB1944	pDR76			DH5a......		pDR76	"2micron, GAL-HA1-SPT3 M188V
FB2352	pLexA-SNF2	Brehon Laurent		XL1 Blue..		pLexA-SNF2	"2micron, HIS3, ADH1p-LexA-SNF2
FB2309	pJAM65	Joe Martens		MH1.66....	AmpR	pJAM65	"2micron, HIS3, ADH1prom, hCYCLIN-T1, ADH1term
FB2353	ADH1p-SNF2	Brehon Laurent		XL1 Blue..		ADH1p-SNF2	"2micron, LEU2, ADH1p-SNF2
FB2036	pMB284	Munrice Basrai		?.5a......		pMB284	"2micron, LEU2, AmpR, ADH1p-HA1-SPT4
FB2253	Gal1-FLP1	Snyder lab	to cure endogenous 2micron from yeast strain	?.........		YEp351-Gal-FLP1	"2micron, LEU2, Gal1p-FLP1
FB1960	pDR63			DH5a......		pDR63	"2micron, LEU2, NHP6b
FB2254	pSE1111	C. Brandl		MH1.......		pSE1111	"2micron, LEU2, SNF4
FB1949	pDR23			DH5a......		pDR23	"2micron, LEU2, SPT3 M188V
FB1948	pDR21			DH5a......		pDR21	"2micron, LEU2, SPT3+
FB1950	pDR25			DH5a......		pDR25	"2micron, LEU2, SPT3-D(DR)
FB2255	pSE1112	C. Brandl		MH1.......		pSE1112	"2micron, TRP1, SNF1
FB1999	pJEF2017	Jeff Boeke		?.5a......		pJEF2017	"2micron, TRP1, URA3, CUP1-TY
FB2094	GHB133	Greg Prelich		DH5a......		pGP211	"2micron, URA3 D213A-bur1
FB2010	JMB1	J Madison	see notebook	DH5a......		pJM1	"2micron, URA3, coSPT3
FB1955	pDR79			DH5a......		pDR79	"2micron, URA3, GAL-SPT3D (DR)
FB2093	GHB129	Greg Prelich		DH5a......		PGP194	"2micron, URA3, HA1-D213A-BUR1
FB2092	GHB132	Greg Prelich		DH5a......		pGP210	"2micron, URA3, HA232-BUR1
FB2031	pGH85	G. Hartzog		MH1.......		pGH85	"2micron, URA3, Leu2-D, GST-SPT6, +1ori
FB2013	JM68	J. Madison	PCR fragment of Spombe SPT3	dh5a......		pJM5	"2micron, URA3, pRS426, SpSPT3 partial
FB1937	pLN138-4	Marian Carlson lab	"ref. Abrams et al, MCB (1986) 6(11) 3643-3651 "	?.1.......		pLN138-4	"2micron, URA3, SNF2, amp
FB1938	pAC153	Marian Carlson's lab	ref. Abrams et al. MCB (1986) 6(11) 3643-3651	..1.......		pAC153	"2micron, URA3, SNF5, amp
FB2015	JMB18	J Madison	see notebook	HB101.....		pJM15	"2micron, URA3, SpSPT3
FB2096	GHB131	Greg Prelich		DH5a......		pMS4	"2micron, URA3,ARSH4,3XHA-BUR1+
FB2307	pJAM63	Joe Martens		MH1.66....	AmpR	pJAM63	"2micron,TRP1,ADH1prom,hCDK9,ADH1term
FB2130	JMB76	L.Guarent	SpTBP in pRS426 Nature 346:291-294	DH5a......			"2micron,URA3 SpTBP
FB2127	pJM15	J.Madison	in pRS426	HB101.....		pJM15	"2micron,URA3, Spombe SPT3
FB2122	pJM1	J. Madison	R1 fragment of Clarispora opuntiae in pRS426	DH5a......		pJM1	"2micron,URA3,CoSPT3
FB2123	pJM2	J.Madison	Xho/H3 subclone of FB2122 in pRS426	DH5a......		pJM2	"2micron,URA3,CoSPT3
FB2139	pJM126	J.Madison		DH5a.DE3..		pJM126	"2micron,URA3,GALprom-GST-SPT3
FB2124	pJM3	J.Madison	Kluyueromyces lactis R1 fragment in pRS426 comp. spt3D203	DH5a......		pJM3	"2micron,URA3,KlSPT3
FB2125	pJM4	J.Madison	subclone of FB2125 in pRS426	DH5a......		pJM4	"2micron,URA3,KlSPT3
FB2294	pJR104	Danesh Moazed	yEp24 based plasmid	DH5a......		pJR104	"2micron,URA3,SIR3
FB2319	3HA-HIS3	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-3HA-His3MX6	"3HA, ADH1term, His3MX6
FB2317	3HA-kanMX6	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-3HA-kanMX6	"3HA, ADH1term, kanMX6
FB2318	3HA-TRP1	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-3HA-TRP1	"3HA,ADH1term, TRP1
FB516		Christy		HB101.....		pBR322 SPT16 BamHI	"4.36 BamHI fragment class I""
FB2276	pLY50	Brehon Laurent	"(1990) MCB 10: 5616-5625, pLY50 is same as pLY14 "	DH5a......		pLY50	"4.8kb R1-BamH1 frag. from SNF5, pRS316
FB2259	pEV374	Eric Verdin	same as pEV229 except HIV enhancer replaced by 5 Gal4 binding sites	HB101.....		pEV374	"5 Gal4 b.s., HIV-LTR, pGL2-Basic
FB2298	pIP49	Ines Pinto	pRS425	DH5a......		pIP49	"ACT3,2micron,LEU2
FB2046	ADA1KO	Guarente Lab	MCB 17:3220-8 (1997)	DH5a......		ADA1KO	"ADA1, hisG, URA3
FB2045	YcP50-ADA1	Guarente Lab	MCB 17:3220-8(1997)	DH5a......		YcP50-ADA1	"ADA1, URA3, CEN
FB1831	pPS794	P. Silver lab	pRS425 with ADE3 cloned in Spe1/BamH1 sites	MH1.......		pPS794	"ADE3, LEU2, 2micron
FB1650	pPS793	P. Silver lab	ADE3 in pRS424 Spe1/BamH1 sites see pPS794 for unique sites used for secto ring assays.**	DH5-alpha		pPS793	"ADE3, TRP1, 2micron, ampR
FB2059	pSR86	SR		MH1.......		pSR86	"ADE3,URA3,2micron,SPT20
FB2037	pDM363	David McNabb		DH5a......		pDM363	"ade3DhisG, URA3
FB2183	pJT10	David Amberg	based on YEplac195	?.B4......		pJT10	"ADE8, URA3
FB2008		struhl lab	RPD3 wild type in YCplac111	DH5a......		RPD3 wt	"ADH prom. RPD3, CEN, LEU2
FB2041	GHB117	Munira Basrat	"SUPT4H PCR cloned into pAD5, MCB 16(6) 2848 "	MH1.......		pMB299	"ADH1 prom HA-SUPT4H, LEU2, 2micron, AmpR
FB2400	pPC97	Steve Buratowski		DH5a......		pPC97	"ADH1p, Gal4 binding domain, LEU2, CEN
FB275		Clare and Farabaugh	Ty-lacZ fusion	HB101.....		pJCdel44	"amp, 2 micron, Ty-lacZ
FB905		Suzanne Sandmeyer	"Ty3 probe; MCB 8, 5245 "	HB101.....		pTy3-2	"amp, F1 ori, pBR322 ori, T7 promoter, Ty3-2
FB31		Winston	"same as pFW11, but opposite lacZ orientation "	DB6507....		pFW12	"amp, HIS4, URA3, lacZ
FB1798	GHB97	CKane Lab		DH5-Alpha.		pJD5	"Amp, LEU2, CRN, ARS, pp22
FB85		Bik Tye	"MboI paritals into Bam site of pCh1. Blue in haploid, white in diploid. "	HB101(?)..		pCh1-41	"amp, LEU2, lacZ, Ty
FB123		Winston	partial evictant of lys2-173R2	HB101.....		pFW48	"amp, part of lys2-173R2
FB1825	B3259	Haoping Liu & Gerry Fink	$.8 kb insert containing FLO8 isolated from HL's genomivc library in pRS316	DH10B.....		pHL1	"amp, puc orig, f1 ori, URA3, FLO8
FB1824	pTW031	Ted Weinert		HB101.....		pTW031	"amp, rad9d::Leu2
FB29		Johnson and Abelson	"SUP6 is an Alu2 fragment with Bam linkers, 750 bp long, tRNAtyr "	DB6507....		pYSUP6-Alu2	"amp, SUP6
FB2033	GHB76	Nomura Lab		DH5a......		HA3982	"amp, SUPT6H
FB92	B161	Fink	"Ty probe, internal "	?.6507....		B161	"amp, Ty 1.2 BglII fragment
FB1002	SL96-33	Patty Minehart	SalI-Hinf frag of GLN1 cloned into pSLFd178k (394bp)	HB101.....		pSL96-33	"ampR GLN1 promoter (UAS only, noTATA)
FB1190	Struhldoub	Struhl	mutant TATA seq= TATATAC REF: MCB dec89 p.5298-5304 Harbury and Struhl * SEE NOTEBOOK!*	HB101.....		p215/238	"ampR URA3 his3-215,238 integrating plasmid
FB1039	pKA20	Karen Arndt	spt15-307= L205F (spt15-122) and F207L. F207L was introduced into the spt15 -122 gene to make a double mut.; the mutagenized XbaI-BamHI frag from spt1 5-122 was then subcloned into pKA12.	HB101.....		pKA20	"AmpR URA3 spt15-307 (L205F, F207L)
FB1041	pKA22	Karen Arndt	spt15-308= L114F and L205F. The spt15-122 (L205F) XbaI-BamHI frag was subcl oned into pKA14 (contains spt15-301=L114F) to replace its XbaI-BamHI sec. pKA22 carries double L to F mutations in the repeats of the SPT15 gene.	HB101.....		pKA22	"AmpR URA3 spt15-308 (L114F, L205F)
FB1468	pGH81	Hovasse	spt4 with Cys24 to His and Cys27 to His double mutation made by PCR from BM construct (FB1131) vector= pRS406 (confirmed by seq.)	HB101.....		pGH81	"ampR URA3 spt4-C24H,C27H
FB1843	pGC26	Boeke lab	integrating marker	..........		pGC26	"ampR, 2 micron
FB1838	pRS402	Boeke lab		..........		pRS402	"ampR, Ade2,
FB1840	pRS422	Boeke lab		..........		pRS422	"ampR, Ade2, 2 micron
FB1829	pSR134	SR		MH1.......		pSR134	"ampR, B42, -gal11
FB1828	pSR133	SR		MH1.......		pSR133	"ampR, B42, -Sin4, TRP1, 2 micron
FB1830	pSR135	SR		MH1.......		pSR135	"ampR, B42, -Snf2
FB1827	pSR132	SR		MH1.......		pSR132	"ampR, B42, RSR1
FB1839	pRS412	Boeke lab		..........		pRS412	"ampR, CEN/ARS, ADE2
FB1842	pGC25	Boeke lab	no s. cerevisiae selectable marker	..........		pGC25	"ampR, CEN6/ARS4
FB1832	pSR78	SR		MH1.......		pSR78	"ampR, gst-Spt20/Ura3/2 micron/leu2d
FB1834	pSR138	SR		MH1.......		pSR138	"ampR, HA1-Spt3/ARG4/cen
FB1841	pRS400	Boeke lab	no s.cerevisiae marker. KanMX is in opposite orientation from sequence provided by https://www.ncbi.nlm.nih.gov/nuccore/U93713.1. Confirmed by PCR using internal primers and corresponding flanking primers after integration into the genome	..........		pRS400	"ampR, Kanmx4neomycin (G418)
FB2042	GHB119	Snyder Lab vis Fink Lab	for rescue of Snyder library URA+ insertions	..........		pRSQLEU2	"ampR, LEU2
FB1794		Dave Rose .	MunI-HIII fragment from p36-1 blunted and cloned into pRS415/SMA1	MH1		pYEZ4-17	"AmpR, LEU2, CEN, ARS, CHD1
FB1835	pRS401	Boeke lab		..........		pRS401	"ampR, Met15
FB1837	pRS421	BOEKE LAB		..........		pRS421	"ampR, Met15, 2micron
FB1836	pRS411	BOEKE LAB		..........		pRS411	"ampR, MET15, CEN/ARS
FB1996	BHM271	Fink Lab		?.5a......		BHM271	"ampR, ori, CEN, HIS3, STE11-4
FB1995	BHM270	Fink Lab		?.5a......		BHM270	"ampR, ori, STE11-4, LEU2, CEN
FB1998	B2616	Fink Lab		DH5a......		B2616	"ampR, pBRori, URA3, STE11-4, CEN
FB1851	pcI-13	E. Dubois	see reference in book	?.........		pcI-13	"ampR, tec1::URA3
FB1853	pNKY1009	N. Kleckner lab	ref. Genetics 116:541-545(1987)	DH5a......		pNKY1009	"ampR, Trp1::hisG-URA3-hisG, ARS1,
FB1793		Grant Hartzog	derivation: pYEZ4-17/Bam/HIII into pRS426/Bam/HIII	MH1.......		pGH187	"AmpR, URA3, 2micron, CHD1
FB1852	p6/50	Stan Field via Elaine Elion		?.........		P6/50	"ampR, URA3, CEN, STE12
FB1848	pIL35	E. Dubois	see reference in book	?.........		pIL35	"ampR, URA3, CEN4/ARS1, Ty Region A, TY2 PvuII-Sau3, TDH3 DUAS-la cZ
FB1849	pIL37	E. Dubois	see reference in book	?.........		pIL37	"ampR, URA3, CEN4/ARS1, Ty Region A- TDH3 DUAS-lacZ, Ty2=PAE1-6, PvuII-Sau3
FB1850	pIL40	E. Dubois	see reference in book	?.........		pIL40	"ampR, URA3, CEN4/ARS1, Ty REgion-A, PvuII-Sau3 Ty1 +PAE2-6, TDH3 DUAS-lacZ
FB1847	pIL30	E.Dubois	see refrence in book	?.........		pIL30	"ampR, URA3, CEN4/ARS1, Ty RegionA-, TDH3 DUAS-lacZ, PvuII-SAU3
FB1855	MPY3XMYC	Brant Schneider	Vector for MYC tagging your favorite gene	DH5a......		MPY3xMYC	"ampR, URA3, PUCori
FB3	B44/YIp5	G.R. Fink	"plasmid very unstable in yeast, even when selected "	DB6507....	HB101 pyrF74::Tn5	YRp10	"ARS1, URA3, amp
FB2091	GHB127	Greg Prelich		DH5a......		pGP111	"BUR1,CEN,URA3
FB2090	GHB128	Greg Prelich		DH5a......		pGP112	"BUR1,URA3, 2micron
FB2369	pGP258	Greg Prelich		DH5a......		pGP258	"BUR6, CEN, URA3
FB2368	pGP256	Greg Prelich		DH5a......		pGP256	"BUR6, URA3, YCP50
FB2370	pGP273	Greg Prelich		DH5a......		pGP273	"bur6D::TRP1, CEN, URA3
FB1982		Irina Derkatch		DH5a......		pEMBL-D3ATG	"CEN, 2micron, SUP35D3ATG
FB1795		Dave Rose .	genmic clone from Hieter library that contains CHD1 and PAB1	MH1		p36-1	"CEN, ARS, LEU2, AmpR, CHD1, PAB1
FB1826	pAB71	Alex Bortvin		HB101.....		pAB71	"CEN, ARS1, LEU2, DED1::GCN4
FB2310	pJAM66	Joe Martens		MH1.66....	AmpR	pJAM66	"CEN, HIS3, ADH1prom, hCYCLIN-T1, ADH1term
FB1963	A2Nde	Guarente Lab		DH5a......		A2Nde	"CEN, Leu2, ADA2
FB1962	pA3P2L	Guarente Lab		DH5a......		pA3P2L	"CEN, LEU2, ADA3
FB1951	pDR32			DH5a......		pDR32	"CEN, LEU2, GAL-HA-SPT3
FB1946	pDR18			DH5a......		pDR18	"CEN, LEU2, GAL-SPT3 M188V
FB1945	pDR16			DH5a......		pDR16	"CEN, LEU2, GAL-SPT3+
FB2160	pJM228	JMadison		DH5a......		pJM228	"CEN, LEU2, Glu-Glu tagged MOT3
FB2159	pJM227	JMadison		DH5a......		pJM227	"CEN, LEU2, Glu-Glu tagged MOT3 (pRS315)
FB1952	pDR34			DH5a......		pDR34	"CEN, LEU2, NHP6a
FB1956	pDR49			MH1.......		pDR49	"CEN, LEU2, NHP6a
FB1959	pDR61			DH5a......		pDR61	"CEN, LEU2, NHP6b
FB2293		Danesh Moazed		DH5a......		pRS315-SIR3	"CEN, LEU2, SIR3
FB2301	pGN1101	Boeke Lab	SpeI/HindIII SPT10 genomic fragment in pRS415	epicurean.	ampR	pGN1101	"CEN, LEU2, SPT10
FB1947	pDR20			DH5a......		pDR20	"CEN, LEU2, SPT3 Deletion DR
FB2262	pRS-SWI2	S.Biggar	"ref Peterson NAR 1996, V.24 No.19 3685-3692 "	DH5a......		pRS-SWI2	"CEN, LEU2, SWI2
FB2263	pRS6-3	S. Biggar	Embo V18 no8 2254-2264	DH5a......		pRS6-3	"CEN, LEU2, Swi2 mts in ATPase domain T909A, P943L, E1077G
FB2308	pJAM64	Joe Martens		MH1.66....	AmpR	pJAM64	"CEN, TRP1, ADH1prom, hCDK9, ADH1 term
FB2028	pRM430	Grunstein Lab	"Mann & Grunstein, EMBO 11:9,3297 "	..........		pRM430	"CEN, TRP1, HHT2 D4-30
FB2027	pRM435	Grunstein Lab	"Mann & Grunstein, EMBO 11:9,3297 "	..........		pRM435	"CEN, TRP1, HHT2 D4-35
FB2025	pRM440	Grunstein Lab	"Mann & Grunstein, EMBO 11:9,3297 "	..........		pRM440	"CEN, TRP1, hht2 D4-40
FB2023	pRM200	Grunstein lab	"Mann & Grunstein, EMBO 11:9,3297 "	..........		pRM200	"CEN, TRP1, HHT2, HHF2
FB2264	pHZ5	Heng Zhou		DH5a......		pHZ5	"CEN, URA3
FB1968	pCDNA18			HB101.....		pCDNA18	"CEN, URA3
FB2246	BM3076	Mark Johnston		DH5a......	'	BM3076	"CEN, URA3, 3XMyc::MIG1
FB1977	pCDNA94			HB101.....		pCDNA94	"CEN, URA3, DIG1
FB1974	pCDNA79			HB101.....		pCDNA79	"CEN, URA3, GAL, TYE7
FB1973	pCDNA69			HB101.....		pCDNA69	"CEN, URA3, GAL-BMH2
FB1976	pCDNA88			HB101.....		pCDNA88	"CEN, URA3, GAL-cMD1
FB1972	pCDNA66			HB101.....		pCDNA66	"CEN, URA3, GAL-URA5
FB1975	pCDNA80			HB101.....		pCDNA80	"CEN, URA3, GAL-URA5
FB1971	pCDNA32			HB101.....		pCDNA32	"CEN, URA3, GAL-YDL084W
FB1970	pCDNA31			HB101.....		pCDNA31	"CEN, URA3, GAL-YLL022C
FB1978	pCDNA103			HB101.....		pCDNA103	"CEN, URA3, RAD23
FB1979	pCDNA50			HB101.....		pCDNA50	"CEN, URA3, RPS12
FB2055	pSR51	SR		MH1.......		pSR51	"CEN, URA3, SPT20
FB1969	pCDNA24			HB101.....		pCDNA24	"CEN, URA3, YNL027W
FB2286	pGY107	Jan Fassler		?.101.....		pGY107	"CEN,URA3,MYC-MCM1
FB2157	pJM190	JMadison		DH5a......		pJM190	"CKS1, CEN, URA3 (pRS316)
FB2164	pJM191	JMadison		DH5a......		pJM191	"CKS1, pRS316, URA3 CEN
FB2017	JMB169	J Madison	see notebook	DH5a......		pJM137	"CoSPT3, pMET25, LEU2, 2 micron,
FB2180	pJYC1513	Arno Greenleaf		unknown...		pJYC1513	"CTK1, pRS316
FB2292	RC4	Jef Boeke	Cup1 promoter plasmid	DH5a......		RC4	"Cup1 prom, galK, Cyc1 term, pBR322
FB2007		struhl lab	gcn5 null	DH5a......		YCplac111Dgcn5	"Dgcn5::LEU2,
FB2006		Struhl Lab	UME6 null	DH5a......		pUC19-DUME6::LEU2	"DUME6,LEU2
FB11	none	Winston	insert is 5.3 kb	B28.32....	trp B1 his (P2 eductant)	pFW1	"E. coli hisOGD H3 in YIp5, amp
FB12	none	Winston	opposite orientation of pFW1	B28.32....	trp B1 his (P2 eductant)	pFW2	"E. coli hisOGD in YIp5, amp, weak tet
FB748		Charlie	polylinker at +272 for transcription	HB101.....		pCHY21	"fbp1', pombe ars, amp, URA3
FB2186	pHL11	G. Fink	"cut with BglII to integrate, See notebook for ref "	DH5a......		pHL11	"FLO8, URA3, Yip pRS316
FB107	B622	Collaborative	GAL UAS with Sal cloning site	?.........			"GAL UAS, URA amp 2 micron
FB2060	pSR90	SR		MH1.......		pSR90	"gal-SPT20,2micron, LEU2d FAT vector
FB2001	pRS426	Berger lab		DH5a......		pRS426	"gal1-ADA2, URA3, 2micron
FB2002	pRS425	Berger Lab	JBC 271:9(1996)	DH5a......		pRS425	"gal1-GCN5, LEU2, 2 micron, ampR
FB2078	pSR129	Shannon Roberts		MH1.......		pSR129	"GAL11, LEU2(pRS305)
FB2082		Berger Lab		DH5a......		A/C GV	"gal4-VP16 Leu2, CEN
FB62		R. Zitomer		E. coli...		YRpBCR6	"galK, uses galK ATG
FB2316	GFPHIS3MX6	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-GFP-HIS3MX6	"GFP(S65T),ADH1term,HIS3MX6
FB2314	GFP-kanMX6	Mark Longtine	see notebook for more information	DH5a......		pFA6a-GFP-kanMX6	"GFP(S65T),ADH1term,kanMX6
FB2315	GFP-TRP1	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6A-GFP-TRP1	"GFP(S65T),ADH1term,TRP1
FB2325	GST-His3	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-GST-His3MX6	"GST, ADH1term, His3MX6
FB2323	GST-kanMX6	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-GST-kanMX6	"GST, ADH1term, kanMX6
FB2324	GST-TRP1	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-GST-TRP1	"GST, ADH1term, TRP1
FB2346	CKB36	Craig Kaplan	BglII-BglII frag from LD10265	DH5a......		pCK36	"GST-DmSPT5, pGEX4T-1
FB2347	CKB37	Craig Kaplan	BglII-BamHI fragment from LD10265	DH5a......		pCK37	"GST-DmSPT5, pGEX4T1
FB2349	CKB43	Craig Kaplan	BglII-XhoI fragment from LD10265	DH5a......		pCK43	"GST-DmSPT5, pGEX5X-1
FB2350	CKB44	Craig Kaplan	BglII-XhoI fragment from LD10265	DH5a......		pCK44	"GST-DmSPT5, pGEX5X-1
FB2344	CKB34	Craig Kaplan	BglII-PvuII DmSPT6 from LD17780	MH1.......		pCK34	"GST-DmSPT6, pGEX1
FB2345	CKB35	Craig Kaplan	BglII-EcoR1 frag from LD17780	MH1.......		pCK35	"GST-DmSPT6, pGEX1
FB2348	CKB38	Craig Kaplan	EcoR1-PvuII fragment from LD02327	DH5a......		pCK38	"GST-DmSPT6, pGEX5X-1
FB1845	pSR72	SR	Bacterial GST-Spt20 expression vector N-terminal	Tm........		pSR72	"GST-Spt20, AA 15-166
FB1846	pSR73	SR	"Bacterial GST-Spt20 expression vector, C-terminus "	BL21......		pSR73	"GST-Spt20, AA437-604
FB2154	pJM224	J.Madison		DH5a......		pJM224	"HA-Human SPT3, pBabe pwo
FB481		Mitchell Smith	see notebook	HB101.....		pMS202	"HHF1, histone H4
FB480		Mitchell Smith	see notebook	HB101.....		pMS201	"HHT1, histone H3
FB2420	pAAD3	Andrea Duina		DH5a......		pAAD3	"hht2-3-HHF2 TRP1, CEN
FB2415	pNOY436	Nomura Lab	ref PNAS v.94 pp.13458-13462 1997	DH5a......		pNOY436	"HHT2-mycHHF2, TRP1, CEN
FB2328	His3-PGAL1	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-His3MX6-PGAL1	"His3MX6, GAL1 promoter
FB2331	His3GALHA	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-HIS3-PGAL1-3HA	"His3MX6, GAL1prom, 3HA, ADH1term
FB2337	His3GALGFP	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		His3MX6-PGAL1-GFP	"His3MX6, GAL1prom, GFP(S65T), ADH1term
FB2334	His3GALGST	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		His3MX6-PGAL1-GST	"His3MX6, GAL1prom, GST, ADH1term
FB2363	pAFS135	Aaron Straight	ref Current Biology 6:1599-1608 1996	Stable2...		pAFS135	"HIS3p-GFP12-LacI-I12, pRS303
FB1348	pLG40	Lisa Gansheroff	E. coli is SICK! See notebook.	HB101.....		pLG40	"his4-912d::URA3, lacZ ampR
FB1349	pLG41	Lisa Gansheroff	E. coli is SICK! See notebook.	HB101.....		pLG41	"his4-912d::URA3, lacZ ampR
FB2076	pPK102	Paul Kaufman	ko construct for CAC1	DH5a......		pPK102	"hisG-URA3-kanR-hisG, ampR
FB479		M. Smith .th/U.Va.	"from Mitchell Smith, see notebook "	HB101.....		pMS191	"histones H3 and H4, copy 1, HHT1 HHF1
FB2258	pEV229	Eric Verdin	vector from Promega	HB101.....		pEV229	"HIV-LTR, pGL2-Basic
FB2392	M1063	David Stillman		DH5a......		M1063	"HO, HIS3, pBR322
FB2391	M598	David Stillman		DH5a......		M598	"HO, URA3, CEN, YCp50
FB2342	p32	Alan Goldstein	"Hygromycin B resistance, Yeast 15, 1541-1553 (1999) "	DH5a......		p32	"hphMX4, pFA6 vector backbone
FB1743	pJH138	J. Hirschhorn	"=pJH79 (FB1703) MunI-SpeI insert into pJH106 (FB1727) MunI-SpeI vector. hta 1-200,1140= amino acid changes S20F and H114Q in HTA1. "	HB101.....		pJH138	"hta1-200,1140 HTB1 HIS3 CEN ampR
FB1754	pJH151	J. Hirschhorn	"=pJH109 (FB1729) SacII-SpeI insert into pRS423 SacII-SpeI vector. hta1-200, 1170= amino acid changes S20F and L117S in HTA1. "	DH5alpha..		pJH151	"hta1-200,1170 HIS3 2micron ampR
FB1729	pJH109	J. Hirschhorn	"=pJH79 (FB1703) MunI-SpeI insert into pJH67 (FB1692) MunI-SpeI vector. hta1 -200,1170= amino acid changes S20F and L117S in HTA1. "	HB101.....		pJH109	"hta1-200,1170 HTB1 HIS3 CEN ampR
FB1742	pJH137	J. Hirschhorn	"=pJH79 (FB1703) MunI-SpeI insert into pJH69 (FB1694) MunI-SpeI vector. hta1 -200,1210= amino acid changes S20F and K121stop in HTA1. "	HB101.....		pJH137	"hta1-200,1210 HTB1 HIS3 CEN ampR
FB1761	pJH158	J. Hirschhorn	"=pJH157 (FB1760) SacII-SpeI insert into pRS423 SacII-SpeI vector. hta1-200, 300= amino acid changes S20F and G30D in HTA1. "	DH5alpha..		pJH158	"hta1-200,300 HIS3 2micron ampR
FB1760	pJH157	J. Hirschhorn	"=pJH142 (FB1747) MunI-XbaI insert into pJH55 (FB1689) MunI-XbaI vector. hta 1-200,300= amino acid changes S20F and G30D in HTA1. "	DH5alpha..		pJH157	"hta1-200,300 HTB1 HIS3 CEN ampR
FB1747	pJH142	J. Hirschhorn	"=pJH75 (FB1699)-- S20F by site directed mutagenesis (dut ung). hta1-200,300 = amino acid changes S20F and G30D in HTA1. "	HB101.....		pJH142	"hta1-200,300 HTB1 URA3 CEN ampR
FB1748	pJH143	J. Hirschhorn	"=pJH139 (FB1744) BamHI-SacII insert into pRS313 BamHI-SacII vector. hta1-20 0,500= amino acid changes S20F and P50L in HTA1. "	DH5alpha..		pJH143	"hta1-200,500 HTB1 HIS3 CEN ampR
FB1744	pJH139	J. Hirschhorn	"=pJH79 (FB1703) BstXI-SpeI insert into pJH133 (FB1738) BstXI-SpeI vector. h ta1-200,500= amino acid changes S20F and P50L in HTA1. "	HB101.....		pJH139	"hta1-200,500 HTB1 URA3 CEN ampR
FB1749	pJH144	J. Hirschhorn	"=pJH140 (FB1745) BamHI-SacII insert into pRS313 BamHI-SacII vector. hta1-20 0,540= amino acid changes S20F and T54N in HTA1. "	DH5alpha..		pJH144	"hta1-200,540 HTB1 HIS3 CEN ampR
FB1745	pJH140	J. Hirschhorn	"=pJH79 (FB1703) BstXI-SpeI insert into pJH74 (FB1698) BstXi-SpeI vector. ht a1-200,540= amino acid changes S20F and T54N in HTA1. "	HB101.....		pJH140	"hta1-200,540 HTB1 URA3 CEN ampR
FB1750	pJH145	J. Hirschhorn	"=pJH141 (FB1746) BamHI-SacII insert into pRS313 BamHI-SacII vector. hta1-20 0,750,760= amino acid changes S20F, N75S and K76E in HTA1. "	DH5alpha..		pJH145	"hta1-200,750,760 HTB1 HIS3 CEN ampR
FB1746	pJH141	J. Hirschhorn	"=pJH79 (FB1703) BstXI-SpeI insert into pJH73 (FB1697) BstXI-SpeI vector. ht a1-200,750,760= amino acid changes S20F, N75S and K76E in HTA1. "	HB101.....		pJH141	"hta1-200,750,760 HTB1 URA3 CEN ampR
FB1758	pJH155	J. Hirschhorn	"=pJH113 (FB1733) SacII-SpeI insert into pRS423 SacII-SpeI vector. hta1-200, 790= amino acid changes S20F and R79G in HTA1. "	DH5alpha..		pJH155	"hta1-200,790 HIS3 2micron ampR
FB1737	pJH126	J. Hirschhorn	"=pJH113 (FB1733) BamHI-SacII insert into pRS313 BamHI-SacII vector. hta1-20 0,790= amino acid changes S20F and R79G in HTA1. "	DH5alpha..		pJH126	"hta1-200,790 HTB1 HIS3 CEN ampR
FB1733	pJH113	J. Hirschhorn	"=pJH79 (FB1703) BstXI-SpeI insert into pJH72 (FB1696) BstXI-SpeI vector. ht a1-200,790= amino acid changes S20F and R79G in HTA1. "	DH5alpha..		pJH113	"hta1-200,790 HTB1 URA3 CEN ampR
FB1755	pJH152	J. Hirschhorn	"=pJH110 (FB1730)SacII-SpeI insert into pRS423 SacII-SpeI vector. hta1-200,9 50= amino acid changes S20F and L95S in HTA1. "	DH5alpha..		pJH152	"hta1-200,950 HIS3 2micron ampR
FB1730	pJH110	J. Hirschhorn	"=pJH79 (FB1703) MunI-SpeI insert into pJH77 (FB1701) MunI-SpeI vector. hta1 -200,950= amino acid changes S20F and L95S in HTA1. "	HB101.....		pJH110	"hta1-200,950 HTB1 HIS3 CEN ampR
FB1759	pJH156	J. Hirschhorn	"=pJH136 (FB1741) SacII-SpeI insert into pRS423 SacII-SpeI vector. hta1-201, 1170= amino acid changes S20P and L117S in HTA1. "	DH5alpha..		pJH156	"hta1-201,1170 HIS3 2micron ampR
FB1741	pJH136	J. Hirschhorn	"=pJH91 (FB1714) MunI-SpeI insert into pJH67 (FB1692) MunI-SpeI vector. hta1 -201,1170= amino acid changes S20P and L117S in HTA1. Note: hta1-201 reve als an XbaI site by altering dam site--- see also FB1708. "	HB101.ha..		pJH136	"hta1-201,1170 HTB1 HIS3 CEN ampR
FB1757	pJH154	J. Hirschhorn	"=pJH112 (FB1732) SacII-SpeI insert into pRS423 SacII-SpeI vector. hta1-300, 790= amino acid changes G30D and R79G in HTA1. "	DH5alpha..		pJH154	"hta1-300,790 HIS3 2micron ampR
FB1736	pJH125	J. Hirschhorn	"=pJH112 (FB1732) BamHI-SacII insert into pRS313 BamHI-SacII vector. hta1-30 0,790= amino acid changes G30D and R79G in HTA1. "	DH5alpha..		pJH125	"hta1-300,790 HTB1 HIS3 CEN ampR
FB1732	pJH112	J. Hirschhorn	"=pJH81 (FB1705) MunI-BstXI insert into pJH75 (FB1699) MunI-BstXI vector. ht a1-300,790= amino acid changes G30D and R79G in HTA1. "	HB101.....		pJH112	"hta1-300,790 HTB1 URA3 CEN ampR
FB1719	pJH98	J. Hirschhorn	"=pJH82 (FB1706) SacII-SpeI frag into pRS423 SacII-SpeI. hta1-750,760= amino acid changes N75S and K76E in HTA1. "	TB1.1.....		pJH98	"hta1-750,760 HIS3 2micron ampR
FB1739	pJH134	J. Hirschhorn	"=pJH82 (FB1706) BamHI-SacII insert into pRS313 BamHI-SacII vector. hta1-750 ,760= amino acid changes N75S and K76E in HTA1. "	DH5alpha..		pJH134	"hta1-750,760 HTB1 HIS3 CEN ampR
FB1706	pJH82	J. Hirschhorn	"=pJH73 (FB1697) BamHI-SacII frag into pRS306 BamHI-SacII. hta1-750,760= ami no acid changes N75S anD K76E in HTA1. "	TB1.own...		pJH82	"hta1-750,760 HTB1 URA3 ampR
FB1697	pJH73	Hirschhorn.rn	"hta1-750,760= amino acid changes N75S and K76E in HTA1 "	HB101.....		pJH73	"hta1-750,760 HTB1 URA3 CEN ampR
FB1728	pJH107	J. Hirschhorn	"=pJH81 (FB1705) XbaI-MunI insert into pJH67 (FB1692) XbaI-MunI vector. hta1 -790,1170= amino acid changes R79G and L117S in HTA1. "	HB101.....		pJH107	"hta1-790,1170 HTB1 HIS3 CEN ampR
FB1756	pJH153	J. Hirschhorn	"=pJH111 (FB1731) SacII-SpeI insert into pRS423 SacII-SpeI vector. hta1-790, 950=amino acid changes R79G and L95S in HTA1. "	DH5alpha..		pJH153	"hta1-790,950 HIS3 2micron ampR
FB1731	pJH111	J. Hirschhorn	"=pJH81 (FB1705) MunI-XbaI insert into pJH77 (FB1701) MunI-XbaI vector. hta1 -790,950= amino acid changes R79G and L95S in HTA1. "	HB101.....		pJH111	"hta1-790,950 HTB1 HIS3 CEN ampR
FB2099	pKR28	Kenneth Robzyk		DH5a......		pKR28	"hta2-htb2-Ub HIS3, CEN
FB2098	pKR27	Kenneth Robzyk		DH5a......		pKR27	"hta2-htb2-Ub, HIS3, 2micron
FB2281	pBM2887	Mark Johnston	"see notebook, made by Karl-Dieter Entian "	DH5a......		pBM2887	"hxk2D::LEU2,
FB17	M182pMC593	Casadaban	9.6 kb	M183.7....	del(lacIPOZY)X74 galK galU strA	pMC593	"kan, amp lac
FB2311	kanMX6	Mark Longtine	see notebook for more info.	DH5a......		pFA6a-kanMX6	"kanMX6,
FB2329	kanGalHA	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998)" pFA6a-kanMX6-PGAL1-3HA	DH5a......		pFA6a-KanMX-3HA	"kanMX6, GAl1 prom, 3HA, ADH1term
FB2326	kan-PGAL1	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		pFA6a-kanMX6-PGAL1	"kanMX6, Gal1 promoter
FB2335	kanGALGFP	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		kanMX6-PGAL1-GFP	"kanMX6, GAL1prom, GFP(S65T), ADH1term
FB2332	KanGAlGST	Mark Longtine	"see notebook for more information, Yeast 14, 953-961 (1998) "	DH5a......		kanMX6-PGAL1-GST	"kanMX6, GAL1prom, GST, ADH1term
FB122		G. Walker	pKM101 derivative from Graham Walker	JC2926....	recA13 plus many others; look in strain notebook	pGW1514	"kanR, pKM101 derivative
FB2143	p55	M.Snyder		DH5a.DE3..		p55	"KNS1,p55
FB622		Stratagene	"bluescript, see notebook "	.B101.....		Bluescript m13-	"KS polylinker, bluescript
FB2362	pAFS59	Aaron Straight	ref Current Biology 6:1599-1608 1996	Stable2...		pAFS59	"lac operator repeat, YIPlac128-LEU2
FB2239	YIplac128	Gietz lab		DH5a......		YIplac128	"LacZ, LEU2
FB2034		W. Bandlow lab		?.5a......		puc19-leo::URA3	"leo1D::URA3, puc19 bckbone
FB2032	pAD54	Dr. Wigler's lab		?.1.......		pAD54	"LEU2, 2micron, ADH1-p, ADH1-T
FB1792		Grant Hartzog	derivation:pYEZ417/Bam/HIII into pRS425/Bam/HIII	MH1.......		pGH186	"Leu2, 2micron, CHD1, ampR
FB2236	YEplac181	Gietz lab		DH5a......		YEplac181	"LEU2, 2micron, LacZ
FB2019	JMB172	J Madison	see notebook	DH5a......		pJM140	"LEU2, 2micron, pMET25, SpSPT3
FB2018	JMB168	J Madison	see notebook	DH5a......		pJM136	"LEU2, 2micron, pMET25,KlSPT3
FB6	RB18	Botstein		DB6656....	trp(am) lac(am) pyrF::Mu su-	YIp32	"LEU2, amp
FB7	RB19	Botstein	"Gene 8, 17-24 (1979) "	DB6656....	trp(am) lac(am) pyrF::Mu su-	YIp33	"LEU2, amp
FB1961	pG5B	Guarente Lab		DH5a......		pG5B	"LEU2, CEN, GCN5
FB2044	pY2435	Young Lab		..........		pY2435	"LEU2, CEN, HA1-RPB3
FB2356		Natalie Kuldell		XL1.......		pRS415-TFG2	"LEU2, CEN, TFG2
FB2360	c3.10	Natalie K		DH5a......		pRS415-tfg2c3.10	"LEU2, CEN, tfg2-c3.10
FB2361	c4.5	Natalie K		DH5a......		pRS415-tfg2c4.5	"LEU2, CEN, tfg2-c4.5
FB2358	M1.1	Natalie K		DH5a......		pRS415-tfg2M1.1	"LEU2, CEN, tfg2-M1.1
FB2359	M2.4	Natalie K		DH5a......		pRS415-tfg2M2.4	"LEU2, CEN, tfg2-M2.4
FB2043	pY2431	Young Lab		..........		pY2431	"LEU2, CEN,RPB3
FB2234	YCplac111	Gietz lab		DH5a......		YCplac111	"LEU2, CEN4, LacZ
FB2247	pJM142	Jon Madison		MH1.......		pJM142	"LEU2, MOT3, 2micron
FB2108	pPWDSGS1	Ed Louis	disruption plasmid	?.........		pPWDSGS1	"LEU2, SGS1
FB2030	PSR89	S ROBERTS		MH1.......		PSR89	"LEU2, SPT20
FB2119	pJM206	J. Madison	human yeast SPT3 chimera in p425MET25	DH5a......		pJM206	"LEU2,2micron, Sc1-103aas human 113-317
FB2120	pJM195	J. Madison	human yeast SPT3 chimera in p425MET25	DH5a......		pJM195	"LEU2,2micron,Sc 1-194 aas human 182-317 aas
FB2121	pJM29	J. Madison	human yeast SPT3 chimera in p425MET25	DH5a......		pJM209	"LEU2,2micron,Sc64-337 aa's human 1-79 aa's
FB2140	pJM165	J.Madison		DH5a.DE3..		pJM165	"LEU2,2micron,SPT3 prom HIS6-SPT3
FB2111	pJM196	J Madison	human yeast SPT3 chimera	.H5a......		pJM196	"LEU2,2u,Sc1-258,human 242-317
FB2087	pLP3	L Pacella	SPT16 in pRS305	DH5a......		pLP3	"LEU2,SPT16
FB1813	M1981	D. Stillman		DH5a......		M1981	"lexA, RGR1, His3
FB1812	M1889	D. Stillman		DH5a......		M1889	"lexA, SIN4, His3
FB613		Jodi	see notebook	HB101.....		pJEH24	"LR1del(25-25,24) 912delta 68mer GAL
FB2288	pRS317	Joe Martens		MH1.......		pRS317	"LYS2, CEN, ARS
FB2285	GC41	Bik Tye		HB101.....		GC41	"MCM1, Bluescript KS-
FB2009	pJM65	J. Madison		DH5a......		pJM65	"MOT1, cen, ARS, URA3
FB2340	p25	Alan Goldstein	"nourseothricin resistance, Yeast 15, 1541-1553 (1999) "	DH5a......		p25	"natMX4, pFA6 vector backbone
FB2265	pHZ52	Heng Zhou		MH1.......		pHZ52	"NRG1, URA3, 2micron
FB1993	pJK91	Pam Silver's lab		.B101.....		pJK91	"nuf2::GFP, TRP1
FB1992	pJK67	Pam Silver's lab		.B101.....		pJK67	"nuf2::GFP, URA3
FB1997	BHM406	Fink Lab		?.5a......		BHM406	"ori, ampR, STE11-4, CEN, TRP1
FB2171	pJM232	JMadison		DH5a......		pJM232	"p425MET25, Glu-Glu-MOT3
FB2149	pJM183	J.Madison		DH5a......		pJM183	"p425MET25, HA-ScSPT31-337
FB2168	pJM184	JMadison		DH5a......		pJM184	"p425MET25, HA1-C.OpmtialSPT3
FB2151	pJM186	J.Madison		DH5a......		pJM186	"p425MET25, HA1-K.lactis SPT3
FB2167	pJM198	JMadison		DH5a......		pJM198	"p425MET25, HA1-ScSPT3 1-315aa
FB2150	pJM185	J.Madison		DH5a......		pJM185	"p425MET25, Ha1-SpombeSPT3
FB2166	pJM187	J.Madison		DH5a......		pJM187	"p425MET25, MOT3
FB2117	pJM204	J Madison	human yeast SPT3 chimera	DH5a......		pJM204	"p425MET25, SPT3 Sc1-24 human 43-317aa's
FB2109	pJM131	J. Madison		DH5a......		pJM131	"p425MET25,HsSPT3, LEU2, 2micron
FB2112	pJM197	J. Madison	human yeast chimera	DH5a......		pJM197	"p425MET25,Sc1-315,human293-317
FB2114	pJM210	J. MADISON	human yeast chimera	DH5a......		pJM210	"P425MET25,Sc143-337,human 1-143
FB2115	pJM189	J. MADison	human yeast chimera	DH5a......		pJM238	"p425MET25,Sc194-337,human1-179
FB2116	pJM203	J Madison	human yeast SPT3 chimera	DH5a......		pJM203	"p425MET25,Sc306-337, human 1-289
FB2113	pJM202	J. Madison	HUMAN YEAST CHIMERA	.H5a......		pJM202	"p425MET25,Sc99-337,human1-109
FB2110	pJM208	J. Madison		DH5a......		pJM208	"p425MET25,ScSPT3,2u,LEU2
FB2118	pJM205	J Madison	yeast human chimera	DH5a......		pJM205	"p425MET25,SPT3 Sc1-61 human 80-317aa's
FB2304	pBAD33	Beckwith Lab	for expressing protein in bacteria see notebook	DH5a.rean.	CM res	pBAD33	"pACYC origin, Ara-inducible
FB2194	pDB20LADA1	David McNabb Guarente lab		DH5a......		pDB20LADA1	"Padh, ADA1, LEU2, 2micron
FB2341	p29	Alan Goldstein	"Bialaphos resistance, Yeast 15, 1541-1553 (1999) "	DH5a......		p29	"patMX4, pFA6 vector backbone
FB2283	pPS4	Priya Sudarsanam		DH5a......		pPS4	"pBKSII, Super GFP with SUC2 flanking region
FB2216	PHJM1	B OLSEN		?.5a......		PHJM1	"pBluescript, 5' end of SUPT3H cDNA
FB2302	pBAD18	Beckwith lab	for expressing protein in bacteria see notebook	DH5a.rean.		pBAD18	"pBR322 Ori, Ara-inducible
FB2339	RC4-2	Lisa Pacella	made from FB2292	DH5a......		RC4-2	"pBR322, CUP1promoter, CYC1terminator
FB2296	pCK22	Craig Kaplan	Spt5cDNA clone from BDGP via Genome Systems	?.........		pCK22	"pBSIIK+ LD10265, Amp
FB2158	pJM225	JMadison		DH5a......		pJM225	"pBSK+, Glu-Glu tagged MOT3
FB2163	pJM186	JMadison		DH5a......		pJM186	"pBSK+, MOT3
FB2390	pLBS39	R. Sternglanz		DH5a......		pLBS39	"pet17b, SPT21, Amp
FB2386	pLBS41	R. Sternglanz		DH5a......		pLBS41	"pet28, SPT10-HIS, SPT21, Kan
FB2387	pLBS42	R. Sternglanz		DH5a......		pLBS42	"pet28a, His-SPT10(aa15-641) SPT21 Kan