Bacteria

Displaying 1 - 500 of 2807

Strain Name Sort descending	Strain Alias	Source	Comments	Host	Host Markers	Plasmids	Plasmid Markers
	pms4



						pMS37
						psh17-4

	dh5a
	dh5alpha
	dh5a

						pLBS27
						pLBS27
						pMRFW2


		rothstein
						pGH127
						pGH%
						pFW217
						pRM200
						pRM%
							ura3 his3
							snf1
							ura3
							%FLAG%

		Joel
							hml
							%hml%

						pNOY436
				fb2195
						phz1
%FB2114%
DB21	RB39	Botstein	"ura3-18 on Bam fragment, LEU2 on Sal/Xho "	DB6660....	see FB20	pRB39	amp ura3-18 LEU2
FB
FB
fb
FB
FB1	DB6507	Botstein	Can screen for URA3 function	..........	pyrF74::Tn5 and all HB101 markers	none
FB10	SF1032	Froshauer	MC4100 genetic background	SF1032....	srl::Tn10 reca ara relA lac(del) strR
FB100	B506	Jef Boeke	912 evictant with G418R	HB101.....		pJEF177	G418R (kanR) URA3 amp
FB1000	PB148	Patty Minehart	SalI-RI frag of GLN1 cloned into SmaI of pSLFd178k (480bp)	XL1.1.....		pPM72	ampR GLN1 promoter (UAS and TATA(2)) CYC1::lacZ
FB1001	PB149	Patty Minehart	"same SalI-RI frag as FB1000, but in reverse orientation "	XL1.1.....		pPM73	ampR GLN1 promoter (UAS and TATA) CYC1::lacZ
FB1002	SL96-33	Patty Minehart	SalI-Hinf frag of GLN1 cloned into pSLFd178k (394bp)	HB101.....		pSL96-33	"ampR GLN1 promoter (UAS only, noTATA)
FB1003	pPM11	Patty Minehart	GLN3 disrupted with LEU2 in pBR322. Note: cutting with AatII gives 2 frag o f approx. the same size.	HB101.....		pPM11	LEU2 ampR ori
FB1004	35BK	Patty Minehart	"GLN3 disrupted w/ LEU2 (same as in FB1003). AatII frag with Bam linkers on each end, then subcloned into Bam of KS+ plasmid (Bluescript). "	HB101.....		p35BK	LEU2 ampR ori
FB1005	pLGd312s	Cindy Keleher	see enclosed sheets	TB1.1.....		pLGd312s	see enclosed sheets
FB1006	JK1621	Cindy Keleher	see enclosed sheets at FB1005	TB1.1.....		pJK1621	see enclosed sheets at FB1005
FB1007	pLGd312-1	Cindy Keleher	see enclosed sheets at FB1005	TB1.1.....		pLGd312-1	see enclosed sheets at FB1005
FB1008	CK26	Cindy Keleher	See enclosed sheets at FB1005	TB1.1.....		pCK26	see enclosed sheets at FB1005
FB1009	CK30	Cindy Keleher	See enclosed sheets at FB1005	TB1.1.....		pCK30	see enclosed sheets at FB1005
FB101	B524	Weinstock et al	"see Weinstock et al, PNAS 80, 4432, 4436 "	MH1000....	ompB101	pORF1
FB1010	CK24	Cindy Keleher	See enclosed sheets at FB1005	TB1.1.....		pCK24	see enclosed sheets at FB1005
FB1011	AJ101	Cindy Keleher	See enclosed sheets at FB1005	TB1.1.....		pAJ101	see enclosed sheets at FB1005
FB1012	JK1521	Cindy Keleher	See enclosed sheets at FB1005	TB1.1.....		pJK1521	see enclosed sheets at FB1005
FB1013	CK22	Cindy Keleher	See enclosed sheets at FB1005	TB1.1.....		pCK22	see enclosed sheets at FB1005
FB1014	CK25	Cindy Keleher	See enclosed sheets at FB1005	TB1.1.....		pCK25	see enclosed sheets at FB1005
FB1015	pL50	Cindy Keleher	See enclosed sheets at FB1005	TB1.1.....		pL50	see enclosed sheets at FB1005
FB1016	CK23	Cindy Keleher	See enclosed sheets at FB1005	TB1.1.....		pCK23	see enclosed sheets at FB1005
FB1017	B1497	Truehart	pBL101 with BglII(blunt)-BamHI(-542) replacing CYC1 sequences Sma-Bam	HB101.....		pSB231	ampR URA3 FUS1:lacZ CEN ARS ori
FB1018	B1498	Truehart	= FB1017 (pSB231) with 2u HindIII frag from YEp24 replacing cen/ars HindIII frag	HB101.....		pSB234	ampR URA3 2u FUS1:lacZ
FB1019	pKA1	Karen Arndt	SalI frag from YCp701 inserted at SalI site of pUC118. Orientation of inser t is not known.	HB101.....		pKA1	ampR his4-912d
FB102	B525	Weinstock	see above	MH1000....	ompB101	pORF2	"see Weinstock et al PNAS 80, 4432-4436
FB1020	pKA2	Karen Arndt	SalI frag from YCp701-R5 inserted at SalI site of pUC118. Orientation of in sert is not known.	HB101.....		pKA2	ampR his4-912dR5
FB1021	pKA3	Karen Arndt	SalI frag from YCp701-R58 was inserted at the SalI site of pUC118. Orientat ion of insert is not known.	HB101.....		pKA3	ampR his4-912dR58
FB1022	pKA5	Karen Arndt	SalI site was inserted 18bp upstream of 15-21 ATG by in vitro mutagenesis. T he 1164bp SalI-BamHI frag from mutagenized 2.4kb EcoRI- BamHI frag of 15-2 1 was subcloned into SalI/BamHI cut pSP64	HB101.....		pKA5	ampR spt15-21
FB1023	pKA6	Karen Arndt	EcoRI-BamHI frag rescued from FW969-1 containing the spt15-122 allele was su jected to oligo directed mut. to introduce a SalI site 18bp upstream of th e 15-122 ATG. The SalI-BamHI frag from the mut. M13 clone was then insert ed into pSP64.	HB101.....		pKA6	ampR spt15-122
FB1024	pKA7	Karen Arndt	SpeI-BamHI frag (1245bp) of pDE32-1 was inserted into pSP64 that had been cu t with XbaI and BamHI.	HB101.....		pKA7	ampR SPT15+
FB1025	pKA9	Karen Arndt	"using oligo-directed mut., an NdeI site was placed at the SPT15 ATG. The 11 43bp NdeI-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid.* DO NOT GIVE OUT* "	BL21(DE3).	pLysS (is a plasmid carrying the T7 lysozyme gene =it is CmR). BL21(DE 3) is a lamda lysogen contai	pKA9	ampR SPT15+
FB1026	pKA9	Karen Arndt	"Using oligo directed mut., an NdeI site was placed at the SPT15 ATG. The 11 43bp NdeI-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid. SEE FB1025!** "	HB101.E3).		pKA9	ampR SPT15+
FB1027	pKA10/pet	Karen Arndt	"using oligo-directed mut, an NdeI site was placed at the spt15 ATG. The 114 3bp NdeI-BamHI frag. was inserted into pET3b, a T7 overexpressor plasmid. SEE FB1025! "	BL21(DE3).	pLysS (a plasmid carrying the T7 lysozyme gene; it is CmR) BL21(DE3) is a lmabda lysogen containi	pKA10	AmpR spt15-122 CmR
FB1028	pKA10	Karen Arndt	"using oligo-directed mut. an NdeI site was placed at the spt15 ATG. The 114 3bp frag was inserted into pET3b, a T7 overexpressor plasmid. SEE FB1027 ! "	HB101.E3).		pKA10	AmpR spt15-122
FB1029	pKA11	Karen Arndt	"using oligo-directed mut. an NdeI site was placed at the spt15 ATG. The 114 3bp NdeI-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid. SEE FB1025! "	BL21(DE3).	pLysS (a plasmid carrying the T7 lysozyme gene; it is CmR) BL21(DE3) is a lambda lysogen containi	pKA11	AmpR spt15-21 CmR
FB103	B570	Giora Simchen	RI partial evictant oflys2-173 with pSL42-2	HB101?....		pGS49	amp URA3 lys2-173
FB1030	pKA11	Karen Arndt	"using oligo-directed mut. an NdeI site was placed at the spt15 ATG. The 114 3bp Nde-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid. * SEE FB1029!* "	HB101.E3).		pKA11	AmpR spt15-21
FB1031	pKA12	Karen Arndt	The 2.4kb EcoRI-BamHI frag from pDE73-21 was cloned into pFW4 cut with EcoRI and BamHI	HB101.E3).		pKA12	AmpR URA3 SPT15
FB1032	pKA13	Karen Arndt	~6.6kb ClaI-SacI insert rescued from FW969 was cloned into pRS306. The ClaI -SacI insert contains the spt15-122 allele.	HB101.E3).		pKA13	AmpR URA3 spt15-122 (integrating)
FB1033	pKA14	Karen Arndt	"spt15-301= L114F, it was introduced by site-directed mut. into the SalI-XbaI frag of pKA4, which was then introduced into pKA12/SalI-XbaI. "	HB101.....		pKA14	AmpR URA3 spt15-301 (L114F)
FB1034	pKA15	Karen Arndt	"spt15-302= G119V, it was introduced by oligo-directed mut into pKA4 SalI-Xba I frag, which was subcloned into pKA12. "	HB101.....		pKA15	AmpR URA3 spt15-302 (G119V)
FB1035	pKA16	Karen Arndt	"spt15-303= L205A, it was subcloned into pKA12 as XbaI-BamHI frag "	HB101.....		pKA16	AmpR URA3 spt15-303 (L205A)
FB1036	pKA17	Karen Arndt	"spt15-304= L205I, it was created by oligo-directed mut. and was introduced i nto pKA12 as XbaI-BamHI frag. "	HB101.....		pKA17	AmpR URA3 spt15-304 (L205I)
FB1037	pKA18	Karen Arndt	"spt15-305= L205K, it was made by oligo-directed mut. in M13 and was subclone d into pKA12 as XbaI-BamHI frag. "	HB101.....		pKA18	AmpR URA3 spt15-305 (L205K)
FB1038	pKA19	Karen Arndt	spt15-306= G210V is subcloned into pKA12 as XbaI-BamHI frag.	HB101.....		pKA19	AmpR URA3 spt15-306 (G210V)
FB1039	pKA20	Karen Arndt	spt15-307= L205F (spt15-122) and F207L. F207L was introduced into the spt15 -122 gene to make a double mut.; the mutagenized XbaI-BamHI frag from spt1 5-122 was then subcloned into pKA12.	HB101.....		pKA20	"AmpR URA3 spt15-307 (L205F, F207L)
FB104	B577	Giora Simchen	eviction of lys2-128delta R3	HB101?....		pGS64	amp URA3 lys2-128deltaR3
FB1040	pKA21	Karen Arndt	XbaI-BamHI frag fron spt15-122 was subcloned into pKA12 tp replace the wild type XbaI-BamHI frag. spt15-122= L205F.	HB101.....		pKA21	AmpR URA3 spt15-122
FB1041	pKA22	Karen Arndt	spt15-308= L114F and L205F. The spt15-122 (L205F) XbaI-BamHI frag was subcl oned into pKA14 (contains spt15-301=L114F) to replace its XbaI-BamHI sec. pKA22 carries double L to F mutations in the repeats of the SPT15 gene.	HB101.....		pKA22	"AmpR URA3 spt15-308 (L114F, L205F)
FB1042	pKA23	Karen Arndt	2.2kb XbaI-HindIII frag containing LEU2 from pDE48-1 was inserted into pKA12 SpeI-HindIII to replace almost the entire SPT15 reading frame with LEU2.( =spt15 null).	HB101.....		pKA23	AmpR URA3 spt15-102dLEU2
FB1043	pKA24	Ricupero/Arndt	"spt15-309= L114A, it was made by oligo directed mut of SalI-XbaI frag of pKA 4, it was then inserted into pKA12 as the SalI-XbaI frag. "	HB101.....		pKA24	AmpR URA3 spt15-309 (L114A)
FB1044	pKA25	Ricupero/Arndt	"spt15-310= L114A, it was introduced by oligo-directed mut. into SalI-XbaI fr ag of pKA4; SalI-XbaI frag was then cloned into pKA12 to replace wt sequen ces. "	HB101.....		pKA25	AmpR URA3 spt15-310 (L114A)
FB1045	pKA26	Ricupero/Arndt	spt15-311= L114K; it was made by oligo-directed mut. of pKA4 SalI-XbaI frag in M13MP18; the mutagenized SalI-XbaI frag was then cloned into pKA12 to r eplace wt seq.	HB101.....		pKA26	AmpR URA3 spt15-311 (L114K)
FB1046	pKA27	Ricupero/Arndt	spt15-312= G119P; was made by oligo-directed mut. of pKA4 SalI-XbaI frag in M13mp18; the mutagenized frag was inserted as a SalI-XbaI piece into pKA12 to replace wt seq.	HB101.....		pKA27	AmpR URA3 spt15-312 (G119P)
FB1047	pKA28	Ricupero/Arndt	spt15-313= K127A; it was made by oligo-directed mut. of pKA4 SalI-XbaI frag in M13mp18;the mutagenized SalI-XbaI frag was then subcloned into pKA12 to replace wt sequences.	HB101.....		pKA28	AmpR URA3 spt15-313 (K127A)
FB1048	pKA29	Ricupero/Arndt	spt15-314= K127R; it was made by oligo-directed mut of pKA4 SalI-XbaI frag i n M13mp18; the mutagenized SalI-XbaI frag was then subcloned into pKA12 to replace the wt seq.	HB101.....		pKA29	AmpR URA3 spt15-314 (K127R)
FB1049	pKA30	Ricupero/Arndt	"spt15-315= L205D; it was made by oligo-directed mut. of XbaI-BamHI frag of p KA4, it was then inserted into pKA12 as the XbaI-BamHI frag to replace wt seq. "	HB101.....		pKA30	AmpR URA3 spt15-315 (L205D)
FB105	B620	Collaborative	GAL UAS on 0.75 kb fragment; does NOT contain the GAL +1 of transcription	.B101?....		CGE258	amp URA3 2 micron GAL UAS
FB1050	pKA31	Ricupero/Arndt	spt15-316= G210P; it was made by oligo-directed mut. of pKA4 XbaI-BamHI frag in M13mp18; the mutagenized frag was then inserted as the XbaI-BamHIfrag of pKA12 to replace wt seq.	HB101.....		pKA31	AmpR URA3 spt15-316 (G210P)
FB1051	pKA32	Ricupero/Arndt	spt15-317= K218A; it was made by oligo-directed mut of pKA4 XbaI-BamHI frag in M13mp18; the mutagenized frag was then cloned onto pKA12 as the XbaI-Ba mHI frag to replace wt seq.	HB101.....		pKA32	AmpR URA3 spt15-317 (K218A)
FB1052	pKA33	Ricupero/Arndt	spt15-318= K218R; it was made by oligo-directed mut of pKA4 Xba-BamHI frag i n M13mp18; the mutagenized frag was then subcloned into pKA12 to replace w t seq.	HB101.....		pKA33	AmpR URA3 spt15-318 (K218R)
FB1053	pCC11	Christy	~6kb Bgl-Bgl frag from pCC27 cloned into Bam site of YCp50. SPT6+ is in ori entation I.	HB101.....		pCC11	AmpR CEN ARS URA3 SPT6+
FB1054	pSIN1	W. Kruger	pSIN1 has SIN1/SPT2 on a 2.7kb RI-SphI fraf cloned into pMR366. (pMR366 is identical to YCp50 except the pBR322 origin has been replaced with pSC101' s thereby making DNA yeilds low.	MH6.......		pSIN1	SIN1/SPT2 AmpR URA3 CEN ARS
FB1055	pSZ26	Szostak/Wu	REF: Plasmid 2:536-554 Szostak and Wu	HB101.....		pSZ26	AmpR rDNA-BglII-A
FB1056	pSPORT1	Gibco/BRL	Clonig vector	HB101.....		pSPORT1	AmpR see insert
FB1057	pKK5-1	Brosius .	"=plasmid for making RNA markers with E.coli RNA polymerase. Ref: Gene (1984 ) 27, p. 161-172 "	HB101.....		pKK5-1	AmpR
FB1058	pKK34-121	Brosius .	"plamid for making RNA markers wiht E. coli RNA polymerase. Ref: MGG (1985) 199, p. 55-58 "	HB101.....		pKK34-121	AmpR
FB1059	pGAL4CG-	Lue	plasmid for yeast in vitro transcription. REF: Science 246 : 661 (1989) Lue et al.	HB101.....		pGAL4CG-	AmpR
FB106	B621	Collaborative	"GAL UAS on 0.75 kb fragment, Xho cloning site "	?.........			GAL UAS URA3 amp
FB1060	pJR201	J. Rooney	see notebook.	HB101.....		pJR201	spt15-201 AmpR CEN ARS TRP1
FB1061	pJR202	J. Rooney	see notebook.	HB101.....		pJR202	spt15-202 AmpR TRP1 CEN ARS
FB1062	pJR203	J. Rooney	see notebook.	HB101.....		pJR203	spt15-203 AmpR TRP1 CEN ARS
FB1063	pJR204	J. Rooney	see notebook.	HB101.....		pJR204	spt15-204 AmpR TRP1 CEN ARS
FB1064	pJR205	J. Rooney	SEE NOTEBOOK	HB101.....		pJR205	spt15-205 AMPR CEN ARS TRP1
FB1065	pJR206	J. Rooney	SEE NOTEBOOK	HB101.....		pJR206	spt15-206 AmpR TRP1 CEN ARS
FB1066	pJR207	J. Rooney	SEE NOTEBOOK	HB101.....		pJR207	spt15-207 AmpR TRP1 CEN ARS
FB1067	pJR208	J. Rooney	SEE NOTEBOOK	HB101.....		pJR208	spt15-208 AmpR TRP1 CEN ARS
FB1068	pJR209	J. Rooney	SEE NOTEBOOK	HB101.....		pJR209	spt15-209 AmpR TRP1 CEN ARS
FB1069	pJR210	J. Rooney	SEE NOTEBOOK	HB101.....		pJR210	spt15-210 AmpR TRP1 CEN ARS
FB107	B622	Collaborative	GAL UAS with Sal cloning site	?.........			"GAL UAS, URA amp 2 micron
FB1070	pJR211	J. Rooney	SEE NOTEBOOK	HB101.....		pJR211	spt15-211 AmpR TRP1 CEN ARS
FB1071	pJR212	J. Rooney	SEE NOTEBOOK	HB101.....		pJR212	spt15-212 AmpR TRP1 CEN ARS
FB1072	pJR213	J. Rooney	SEE NOTEBOOK	HB101.....		pJR213	spt15-213 AmpR TRP1 CEN ARS
FB1073	pRS104	R. Simpson	See notebook for information in insert.	HB101.....		pRS104	ARS AmpR
FB1074	pRS109	R. Simpson	see notebook for information in insert.	HB101.....		pRS109	REP3 AmpR
FB1075	CP32	C. Peterson	=BglII-SmaI frag of SWI3 in YIp5 (for making Southern probe). See letter in notebook.	unknown...		CP32	SWI3 URA3 AmpR
FB1076	CP34	C. Peterson	is in YEp24. SWI2 (SNF2) disruption allele was created by replacing the lg. BglII frag with HIS3 gene. See letter in notebook for information.	unknown...		CP34	swi2d::HIS3 AmpR URA3 2micron ori
FB1077	CP59	C. Peterson	see letter in notebook for information.	unknown...		CP59	swi3d::TRP1 AmpR URA3
FB1078	BD39	C. Peterson	"is in YIp5. swi1::LEU2 allele was constructed by replacing BamHI/BglII dele tion of SWI1 with LEU2. Digest with PstI, isolate ~1kb frag for one-step d isruption of SWI1. See notebook for letter. "	HB101.n...		BD39	swi1d::LEU2 AmpR URA3
FB1079	pDAD1	Pellman/Fink	See notebook for more info. and polylinker sequence	HB101.....		pDAD1	AmpR URA3 2micron Gal10' promoter
FB108	B625	D. Schild via Fink	Current Genetics 7: 85-92	JA300.....	thr leuB6 thi thyA trpC117 shmK- hsrK- strR	pSM21	rad52::TRP1 amp
FB1080	pDAD2	Pellman/Fink	See notebook for more info. and polylinker sequence.	HB101.....		pDAD2	AmpR URA3 2micron GAL10' promoter
FB1081	pLR1	L. Rosen	"from FB759 (pRB759), URA3 SacI frag cloned into SacI site of FB659 (pRS314) that had its PpuMI site filled in. See FB1082 (pLR2). "	HB101.....		pLR1	AmpR TRP1 URA3 CEN ARS
FB1082	pLR2	L. Rosen	#NAME?	HB101.....		pLR2	AmpR TRP1 CEN ARS
FB1083	pGBT9	Stan Fields	see notebook	HB101.....		pGBT9	TRP1 AmpR Gal4 (1-147)
FB1084	pMFH1	D. Gottschling	REF: MCB vol 5 No 4M Apr85 p.610-618. See notebook	HB101.....		pMFH1	LEU2 URA3 AmpR 2micron DAM
FB1085	pDP6+DAM	D. Gottschling	REF: MCB vol5 No4 Hoekstra + Malone 1985 p.610-618 MCB vol6 No10 Hoekstra + Malone 1986 p.3555-3558. See sheets in notebook.	HB101.....		pDP6+DAM	LYS2 DAM AmpR
FB1086	pRR46	D. Gottschling	REF: MCB 7:1012	HB101.....		pRR46	rad1::LEU2
FB1087	BM1541	Mark Johnston	to cut out GAL4 use either BamHI + HindIII or BamHI + EcoRI; the entire gen e including its promoter is in these frags. See sheets in notebook.	unknown...		pBM1541	AmpR TRP1 CEN ARS GAL4
FB1088	pJR303	J. Rooney	RI-Bam frag of spt15-203 colned into YIp5 vector as its Bam/RI frag.	HB101.n...		pJR303	URA3 AmpR spt15-203
FB1089	pJR305	J. Rooney	Bam/RI frag containing spt15-205 cloned into YIp5 vector as its Bam/RI frag.	HB101.n...		pJR305	AmpR URA3 spt15-205
FB109	B626	D. Schild via Fink	see FB108	JA300.....	see 108	?	rad52::LEU2
FB1090	pJR307	J. Rooney	Bam/RI frag containing spt15-207 was cloned into YIp5 vector as its Bam/RI f rag.	HB101.n...		pJR307	AmpR URA3 spt15-207
FB1091	pJR309	J. Rooney	Bam/RI frag containing spt15-209 was cloned into YIp5 vector as its Bam/RI f rag/	HB101.n...		pJR309	AmpR URA3 spt15-209
FB1092	pJR311	J. Rooney	Bam/RI frag containing spt15-211 was cloned into YIp5 vector as its Bam/RI f rag.	HB101.n...		pJR311	AmpR URA3 spt15-211
FB1093	pJR312	J. Rooney	Bam/RI frag containing spt15-212 was cloned into YIp5 vector as its Bam/RI f rag.	HB101.n...		pJR312	AmpR URA3 spt15-212
FB1094	BDE115	D. Eisenmann	=BDE115 in Dave's collection.	HB101.....		pDE40-4	SPT15 AmpR TRP1 CEN ARS
FB1095	WB101	Craig Peterson	plasmid is in a YCp50 vector	unknown...		pWB101	URA3 AmpR HHT2 HHF2 CEN ARS
FB1096	pM883	David Stillman	*DO NOT GIVE OUT!!!* (see sheet) pM883= SIN3 in YCp50.	HB101.n...		pM883	SIN3 URA3 AmpR CEN ARS
FB1097	pM1387	David Stillman	*DO NOT GIVE OUT!!!* (see enclosed sheet) pM1387= SIN4 in pRS316	HB101.n...		pM1387	SIN4 URA3 AmpR CEN ARS
FB1098	BBM11	Betsy Malone	Sal-Xho of LEU2 in YEp13 inserted into Sal site of pBM8	HB101.....		pBM11	spt16-102::LEU2 AmpR URA3
FB1099	BBM22	Betsy Malone	Bam frag of spt16-102::LEU2 from pBM11 (FB1098) into Bam site of YEp24	HB101.....		pBM22	spt16-102::LEU2 AmpR URA3 TetS 2micron ori
FB11	none	Winston	insert is 5.3 kb	B28.32....	trp B1 his (P2 eductant)	pFW1	"E. coli hisOGD H3 in YIp5, amp
FB110	B636	D. Schild via Fink	see Current Genetics 7:85-92.	HB101.....		YEp13-RAD52	RAD52 LEU2 2 micron amp
FB1100	BBM23	Betsy Malone	H3-Sal frag of SPT16 (from pBM7) put into pBR322.**This frag is probably N ONfunctional---it was made as a NORTHERN PROBE**	HB101.....		pBM23	SPT16 frag AmpR TetS ori
FB1101	BBM25	Betsy Malone	ScaI-H3 frag of SPT4 into H3-NruI site of YCp50	HB101.....		pBM25	SPT4 AmpR URA3 CEN ARS
FB1102	BBM26	Betsy Malone	RI-PvuII of SPT4 into RI-SmaI of YEp355 ***makes an in frame SPT4-lacZ fusio n	HB101.....		pBM26	SPT4-lacZ AmpR URA3 2micron
FB1103	BBM27	Betsy Malone	RI-H3 frag of SPT4 into RI-H3 of pUC19	HB101.....		pBM27	SPT4 AmpR ori
FB1104	BBM33	Betsy Malone	spt16-23 Bam frag into pRS314 (= a derivative of pBM13) Yeast strain=L722 a lias BM101	HB101.....		pBM33	spt16-23 AmpR TRP1 CEN ARS ori
FB1105	BBM34	Betsy Malone	spt16-35 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L723 a lias BM102	HB101.....		pBM34	spt16-35 AmpR TRP1 CEN ARS ori
FB1106	BBM35	Betsy Malone	spt16-99 Bam Frag into pRS314 (=a derivative of pBM13) Yeast strain= L724 a lias BM103	HB101.....		pBM35	spt16-99 AmpR TRP1 CEN ARS ori
FB1107	BBM37	Betsy Malone	spt16-133 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L725 alias BM124	HB101.....		pBM37	spt16-133 AmpR TRP1 CEN ARS ori
FB1108	BBM39	Betsy Malone	spt16-146 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L726 alias BM126	HB101.....		pBM39	spt16-146 AmpR TRP1 CEN ARS ori
FB1109	BBM42	Betsy Malone	spt16-175 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L727 alias BM129	HB101.....		pBM42	spt16-175 AmpR TRP1 CEN ARS ori
FB111	B638	D. Schild via Fink	see Current Genetics 7:85-92	JA300.....	see FB108	pSM13
FB1110	BBM44	Betsy Malone	spt16-181 Bam frag into pRS314 (=a derivative of pBM13) Yeast strain= L728 alias BM131	HB101.....		pBM44	spt16-181 AmpR TRP1 CEN ARS ori
FB1111	BBM45	Betsy Malone	spt16-185 Bam frag in pRS314 (= a derivative of pBM13) Yeast strain= L729 alias BM132	HB101.....		pBM45	spt16-185 AmpR TRP1 CEN ARS ori
FB1112	BBM46	Betsy Malone	spt16-197 Bam frag in pRS314 (= a derivative of pBM13) Yeast strain= L730 a lias BM133	HB101.....		pBM46	spt16-197 AmpR TRP1 CEN ARS ori
FB1113	BBM47	Betsy Malone	SPT4 with Amino acid #7 changed fron Cys to Ser. (in vitro mutagen. of M13 clone 106 with primer BM32. H3 frag subcloned into YCp50)	HB101.....		pBM47	spt4 (Cys7 to Ser) URA3 AmpR CEN ARS
FB1114	BBM49	Betsy Malone	SPT4 with amino acid #7 changed from Cys to His---Has opp orientation as tha t of pBM48. (From in vitro mut. fo M13 clone 106 with primerBM33. H3 fra g subcloned into YCp50.)	HB101.....		pBM49	spt4 (Cys7 to His) URA3 CEN ARS AmpR
FB1115	BBM50	Betsy Malone	SPT4 with amino acid #27 changed from Cys to Ser. (from in vitro mut. of M1 3 clone 106 with primer BM34. H3 frag subcloned into YCp50.	HB101.....		pBM50	spt4 (Cys27 to Ser) AmpR URA3 CEN ARS
FB1116	BBM51	Betsy Malone	from gap repair of pJF42 in L331	HB101.....		pBM51	spt4-289 AmpR URA3 CEN ARS
FB1117	BBM52	Betsy Malone	from gap repair of pJF42 in L351	HB101.....		pBM52	spt4-3 AmpR URA3 CEN ARS
FB1118	BBM53	Betsy Malone	from gap repair of pJF42 in L374	HB101.....		pBM53	spt4-6 AmpR URA3 CEN ARS
FB1119	BBM56	Betsy Malone	NOTE: lerner fusion to the N terminus of SPT4. (from oligo dir. mut. of M13 clone 106 with BM28; H3 frag subcloned into YCp50.)	HB101.....		pBM56	lerner::SPT4 AmpR URA3 CEN ARS
FB112	HB101	via Fink	B324 in GR Fink collection	.A300.....	thr leu pro B1 supE44 r- m- recA
FB1120	BBM57	Betsy Malone	NOTE: lerner fusion to C terminus of SPT4 (from oligo dir mut. of M13 clone 106 with BM29; H3 frag was subcloned into YCp50.	HB101.....		pBM57	SPT4::lerner AmpR URA3 CEN ARS
FB1121	BBM61	Betsy Malone	SPT4 with amino acid #27 changed from Cys to His. (from oligo dir mut. of M1 3 clone 106 with BM35; H3 frag subcloned into YCp50)	HB101.....		pBM61	spt4 (Cys27 to His) AmpR URA3 CEN ARS
FB1122	BBM65	Betsy Malone	NOTE: lerner fusion to the N terminus of SPT4 (H3 frag from mut7C cloned in to H3 site of pCGS42)	HB101 ....		pBM65	lerner::SPT4 AmpR ori URA3 2micron
FB1123	BBM66	Betsy Malone	NOTE: lerner fusion to the C terminus of SPT4 (H3 frag from mut88 cloned int o H3 site of pCGS42.)	HB101.....		pBM66	SPT4::lerner AmpR URA3 2micron ori
FB1124	BBM68	Betsy Malone	insert SPT5 (H3 to KpnI) into YIp356R (Has partial SPT5 function; blue in coli and yeast) E. coli strain is SICK.	TB1.1.....		pBM68	SPT5-lacZ (H3 to KpnI) AmpR URA3
FB1125	BBM72	Betsy Malone	NOTE: lerner fusion to the N terminus of SPT4 (H3 frag from FB1122 [pBM65] cloned into H3 sites of YEp13.)	HB101.....		pBM72	SPT4::lerner AmpR LEU2 2micron TetR
FB1126	BBM76	Betsy Malone	NOTE: =Orientation II. (H3 frag from mut13C into pCGS42)	HB101.....		pBM76	HAI::spt4 (Cys7 to Ser) URA3 2micron AmpR
FB1127	BBM77	Betsy Malone	NOTE: = Orientation I. (H3 frag from mut14C into pCGS42.)	HB101.....		pBM77	HAI::spt4 (Cys7 to His) URA3 2micron AmpR
FB1128	BBM79	Betsy Malone	NOTE: = Orientation I (H3 frag from mut15A cloned into pCGS42.)	HB101.....		pBM79	HAI::spt4 (Cys27 to Ser) URA3 2micron AmpR
FB1129	BBM80	Betsy Malone	NOTE: = Orientaion I (H3 frag from mut16B into pCGS42.)	HB101.....		pBM80	HAI::spt4 (Cys27 to His) URA3 AmpR 2micron
FB113	B522	Silhavy	"PNAS 80,4432 "	MH3000....	"araD139 del(ara,leu)7697 del(lac)X74 galU galK rpsL (strR) ompR101
FB1130	BBM83	Betsy Malone	NOTE: = Orientation II (H3 frag from mut18B into pCGS42.)	HB101.....		pBM83	HAI::spt4 (Cys24 to His) URA3 AmpR 2micron
FB1131	BBM84	Betsy Malone	NOTE: =Orientation II (H3 frag from mut19B into pCGS42.)	HB101.....		pBM84	HAI::spt4 (Cys24; 27 to His) URA3 AmpR 2micron
FB1132	BBM89	Betsy Malone	NOTE: = Orientation I (H3 frag from mut17C into pCGS42.)	HB101.....		pBM89	HAI::spt4 (Cys24 to Ser) URA3 AmpR 2micron
FB1133	pHB51	Henry Baker U. Fla	TPI1= triose phosphate isomerase. H3-H3 frag with TPI1 (~4.4kb) in pUC18.	HB101.....		pHB51	AmpR TPI1
FB1134	pHB59	Henry Baker U. Fla.	TPI1= triose phosphate isomerase. H3-SphI frag of TPI1 into pUC18.	HB101.....		pHB59	AmpR TPI1
FB1135	pMS39	Michele Swanson	from a 7kb H3-EagI frag containing HAI-SPT6 ligated to pRS315 at H3+EagI	TB1.......		pMS39	HAI-SPT6 LEU2 CEN AmpR ARS
FB1136	EB153	M. Tyers + E. Elion	GAL1-CLN3-1 (dominant mutation) fusion	HB101.....		pCGS-CLN3-1	AmpR URA3 2u GAL1::CLN3-1 fusion
FB1137	EB154	M. Tyers + E. Elion	GAL-CLN3 fusion	HB101.....		pCGS-CLN3	AmpR URA3 2u GAL1::CLN3 fusion
FB1138	EB142	E. Elion	fus3d6::LEU2= XmnI deletion XhoI deletion with LEU2 in Xho site. To integra te cut with NheI and HindIII	unknown...		pYEE98	AmpR fus3d6::LEU2
FB1139		Chris Clark	4.3kb BamHI frag containing SPT16 cloned into BamHI site of YEp24	HB101.....		pCC48	SPT16 2u URA3 ampR tetS
FB114	B523	Silhavy		TK1046....	araD139 del(argF-lac)U169 rpsL150(strR) relA1 flbB5301 deoC1 ptsF25 ma lPQ::Tn5 ompBcs1
FB1140		A. Rowley + Curt Wittenburg		HB101.....
FB1141	pMAL-c	NEB	for making fusions to MBP (maltose binding protein) encoded by malE to ove rexpress proteins in E. coli	HB101.....		pMAL-c	AmpR
FB1142	pMAL-p	NEB	for making fusions to MBP (maltose binding protein) encoded by malE to o verexpress proteins in E. coli	HB101.....		pMAL-p	AmpR
FB1143	pMAL-cRI	NEB	for making fusions to MBP (maltose binding protein) encoded by malE To ove rexpress protein in E. coli	HB101.....		pMAL-cRI	AmpR
FB1144	pGN801	G. Natsoulis + J. Boeke	See Genetics 123:269	unknown...		pGN801-ura3-153d	ura3-153d TRP1 CEN AmpR
FB1145	pFC101.1	Cross via Elion	contains 6kb of yeast DNA that includes DAF1-1. It was in YCp50- but Fred C ross deleted CEN.	unknown...		pFC101.1	DAF1-1 (=CLN3?) URA3 ampR
FB1146	p810	Reed via Elion	YRp7 with a HindIII-SalI frag (4kb) containing the rest of CLN2 disrupted by LEU2	unknown...		p810	cln2::LEU2 ampR TRP1 ARS1
FB1147	p419	Reed via Elion	YEp13 with a 3kb EcoRI frag containing most of CLN1 with a TRP1 insertion	unknown...		p419	cln1::TRP1 (weak trp+) LEU2 2micron ampR
FB1148	pPT1	Patrick Tan	*Tagged SPT7*	TB1.......		pPT1	SPT7-HAI LEU2 CEN ARS ampR
FB1149	pPT4	Patrick Tan	Same as FB1148 but 56a.a. repeat sequence deleted by oligo directed mut.	TB1.......		pPT4	SPT7 LEU2 CEN ARS ampR
FB115	?	D. Hanahan	high frequency transformer	?.1046....	?	none
FB1150	pPT3	Patrick Tan	*UNtagged SPT7*	TB1.......		pPT3	SPT7 LEU2 CEN ARS ampR
FB1151	pPT2	Patrick Tan	*tagged SPT7 in dam- strain* see also FB1148	MM572.....	dam- See FB274	pPT2	SPT7-HAI LEU2 CEN ARS ampR
FB1152	pCC58	Chris Clark	4.6kb SPT16 BamHI frag cloned into BamHI site of YCp50 (orientation A)	HB101.....		pCC58	SPT16 URA3 ampR tetS
FB1153	pCC61	Chris Clark	5.7kb SalI frag containing HTA1-HTB1 cloned into SalI site of YEp24.	HB101.....		pCC61	HTA1-HTB1 URA3 ampR 2micron
FB1154	pCC64	Chris Clark	~6.7kb HindIII frag containing HHT1-HHF1 cloned into HindIII site of pCGS42.	HB101.....		pCC64	HHT1-HHF1 2micron URA3 ampR
FB1155	pCC66	Chris Clark	2.6kb HindIII frag w/ HHT2 + 1.48 HindIII frag w/ HHF2 both cloned into Hind III site of pCGS42.	HB101.....		pCC66	HHT2-HHF2 URA3 2micron ampR tetR
FB1156	pCC67	Chris Clark	6.4kb HTA1-HTB1 Bam frag + 5.4kb HHT1-HHF1 Bam-H3 frag were cloned into the H3 and Bam sites of pCGS42.	HB101.....		pCC67	HHT1-HHF1 HTA1-HTB1 2micron URA3 ampR
FB1157	pDE92-4	D. Eisenmann	= high copy spt15-21 with SalI site	HB101.....		pDE92-4	spt15-21 2micron TRP1 ampR
FB1158	pDE93-6	D. Eisenmann	#NAME?	HB101.....		pDE93-6	SPT15 2micron TRP1 ampR
FB1159	pDE105-6	D. Eisenmann	used for gapped rescue of SPT3 alleles by cutting with MluI and XhoI	HB101.....		pDE105-6	SPT3 URA3 CEN ARS ampR
FB116	K38			K38.46....	HfrC
FB1160	pDE118-4	D. Eisenmann	SPT3 cloned into M13 + mut to insert an NdeI site at the ATG. This frag (RI -H3) was cloned into pRS426. has SPT3+ activity	HB101.....		pDE118-4	SPT3 with NdeI site at ATG 2micron URA3 ampR
FB1161	pDE119-1	D. Eisenmann	SPT3 mut in M13 to attach 9 a.a. Lerner epitope at SPT3 N terminal. An RI-H 3 frag containing this was cloned into pRS426. has SPT3+ activity + epit ope tag IN VIVO	HB101.....		pDE119-1	SPT3 with Lerner epitope at NT URA3 2micron ampR
FB1162	pDE120-4	D. Eisenmann	see FB1161 * Lerner tagged SPT3 C terminal* has SPT3+ activity but tag is not seen by Western	HB101.....		pDE120-4	SPT3 with Lerner epitope at CT 2micron URA3 ampR
FB1163	pDE133-12	D. Eisenmann	*Lerner tagged spt3-401*	HB101.....		pDE133-12	spt3-401 with Lerner epitope at NT 2micron URA3 ampR
FB1164	pMR79	Mark Rose .n	#NAME?	HB101.....		pMR79	URA3 CEN ARS ampR
FB1165	L15/pUN45	S. Buratowski	SPT15 with Lerner at N terminal in vector pUN45 (Elledge + Davis)	HB101.....		L15/pUN45	SPT15 with Lerner epitope at NT TRP1 CEN ARS ampR
FB1166	L15/pUN55	S. Buratowski	spt15 with Lerner at N terminal in vector pUN55	HB101.....		L15/pUN55	SPT15 with Lerner epitope at NT URA3 CEN ARS ampR
FB1167	pGP26	Greg Prelich	BglII spt6-140 genomic frag in BamHI site of YCp50.	HB101.....		pGP26	spt6-140 ampR URA3 CEN ARS
FB1168	pAB56	A. Bortvin	BglII spt6-140 frag cloned into BamHI site of YCp50 that has BglII-SmaI frag (CEN ARS) deleted. see also FB1167.	HB101.....		pAB56	spt6-140 URA3 ampR
FB1169	pAB1	A. Bortvin	3.5kb SPT5 frag from pMS4 cloned into SmaI site of pJK1521. LexA-SPT5 fusio n= 12-1064 a.a.	HB101.....	sick	pAB1	SPT5-lexA TRP1 CEN ARS ampR
FB117	JM101	via Fink	This is strain B234 in the G.R. Fink collection	JM101.....	del(lac-pro) supE thi/ F'traD36 proAB lacIq	none
FB1170	pAB57	A. Bortvin	same as FB1169 but fusion of lexA with SPT5 starting at a.a.#632-1064.	HB101.....	sick	pAB57	SPT5-lexA TRP1 CEN ARS ampR
FB1171	pCC74	Chris Clark	URA3 HindIII frag cloned into the trt1 deletion site in pCC72. Note: URA3 o rientation #1.	FB1.1.....	pyrf-	pCC74	(hta1-htb1)d::URA3 ampR
FB1172	MH1	Lorraine Marsh	*Good for transformation with Smash and Grab DNA* see enclosed sheet in notebook for transformation procedure.	MH1.1.....
FB1173	pCC45	Chris Clark	5.5kb Bgl frag of SPT6 cloned into BamHI site of YEp13. (orientation I) H IGH COPY SUPPRESSION	HB101.....		pCC45	SPT6 LEU2 2micron ampR
FB1174	Struhl#wt	Struhl	"TATA mut. in this plasmid= WT, but with RI and Sac sites flanking the HIS3 T ATA.REF= MCB dec89 p.5298-5304 Harbury + Struhl *SEE NOTEBOOK* "	HB101.....		pwt	URA3 ampR his3-RIS integrating plasmid
FB1175	Struhl#201	Struhl	TATA mut. in this plasmid is AATAAA. REF= MCB dec89 p. 5298-5304 Harbury + Struhl. **SEE NOTEBOOK**	HB101.....		p201	URA3 ampR his3-201 integrating plasmid
FB1176	Struhl#202	Struhl	TATA mut.= CATAAA. REF= MCB dec89 p. 5298-5304 Harbury + Struhl *SEE N OTEBOOK*	HB101.....		p202	URA3 ampR his3-202 integrating plasmid
FB1177	Struhl#203	Struhl	TATA mut.= GATAAA. REF= MCB dec89 p. 5298-5304 Harbury + Struhl *SEE N OTEBOOK*	HB101.....		p203	URA3 ampR his3-203 integrating plasmid
FB1178	Struhl#204	Struhl	TATA mut.= TCTAAA REF= MCB dec89 p.5298-5304 Harbury + Struhl *SEE NO TEBOOK*	HB101.....		p204	URA3 ampR his3-204 integrating plasmid
FB1179	Struhl#205	Struhl	mutant TATA seq= TGTAAA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p205	ampR URA3 his3-205 integrating plasmid
FB118	B155	"Fink, Phil Carter	orientation of insert unknown	?.101.....		B155	Ty912 HIS4 URA3 amp
FB1180	Struhl#206	Struhl	mutant TATA seq= TTTAAA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p206	ampR URA3 his3-206 integrating plasmid
FB1181	Struhl#208	Struhl	mutant TATA seq= TACAAA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p208	ampR URA3 his3-208 integrating plasmid
FB1182	Struhl#209	Struhl	mutant TATA seq= TAGAAA REF: MCB dec89 p. 5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p209	ampR URA3 his3-209 integrating plasmid
FB1183	Struhl#210	Struhl	mutant TATA seq= TATCAA REF: MCB dec89 p. 5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p210	ampR URA3 his3-210 integrating plasmid
FB1184	Struhl#212	Struhl	mutant TATA seq= TATTAA REF: MCB dec89 p.5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p212	ampR URA3 his3-212 integrating plasmid
FB1185	Struhl#213	Struhl	mutant TATA seq= TATACA REF: MCB dec89 p.5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p213	ampR URA3 his3-213 integrating plasmid
FB1186	Struhl#214	Struhl	mutant TATA seq= TATAGA REF: MCB dec89 p.5298-5304 Harbury and Struhl S EE NOTEBOOK!	HB101.....		p214	ampR URA3 his3-214 integrating plasmid
FB1187	Struhl#215	Struhl	mutant TATA seq= TATATA REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p215	ampR URA3 his3-215 integrating plasmid
FB1188	Struhl#217	Struhl	mutant TATA seq= TATAAG REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p217	ampR URA3 his3-217 integrating plasmid
FB1189	Struhl#218	Struhl	mutant TATA seq= TATAAT REF: MCB dec89 p.5298-5304 Harbury and Struhl SEE NOTEBOOK!	HB101.....		p218	ampR URA3 his3-218 integrating plasmid
FB119		Winston	Bgl2-Xho internal LYS2 fragment in pBR322; LYS2 probe	HB101.....		pFW47	LYS2 amp
FB1190	Struhldoub	Struhl	mutant TATA seq= TATATAC REF: MCB dec89 p.5298-5304 Harbury and Struhl * SEE NOTEBOOK!*	HB101.....		p215/238	"ampR URA3 his3-215,238 integrating plasmid
FB1191	B1295	Broach Lab		unknown...		pB1295	MATa LEU2 CEN ARS ampR
FB1192	B1311	Broach lab	MATb (alpha) Hind3 cloned from LCIII 3a into YCp50	unknown...		pB1311	MATb URA3 CEN ARS ampR
FB1193	pMS23	M. Swanson	~4kb Hind3-Kpn of pMS4 cloned into Hind3-Kpn of pRS316. SPT5 is truncated in the repeat region; partially comp. spt5-. REF: MCB 11: 3009-3019. 1991	TB1.own...		pMS23	SPT5 URA3 ampR bluescript
FB1194	pJN22	Hans Ronne	#NAME?	HB101.n...		pJN22	mig1-82::LEU2 ampR
FB1195	pJN41	Hans Ronne	#NAME?	HB101.n...		pJN41	mig1-82::URA3 ampR
FB1196	p125-3	D. Eisenmann	~6.3kb Xho-Xho piece from original SPT15 clone-- located 3' to the SPT15 gen e and cloned into pRS316	HB101.n...		p125-3	URA3 CEN ARS ampR
FB1197	pDE18-1	D. Eisenmann	~5.5kb Cla-Bam subclone with SPT15+ activity cloned into Cla-Bam of pRS316 ( see sheet inserted with FB1196)	HB101.n...		pDE18-1	SPT15 URA3 CEN ARS ampR
FB1198	pDE19-1	D. Eisenmann	3.6kb Bam-Cla frag located 3' of SPT15 gene cloned into Bam-Cla of pRS316 (s ee sheet inserted with FB1196)	HB101.n...		pDE19-1	URA3 CEN ARS ampR
FB1199	pDE23-8	D. Eisenmann	~5kb Hind3 piece containing part of SPT15 gene into pRS316 Hind3 site. do es NOT have 15+ activity because the last 13aa are not contained in this f rag. (see sheet inserted with FB1196)	HB101.n...		pDE23-8	URA3 CEN ARS ampR
FB12	none	Winston	opposite orientation of pFW1	B28.32....	trp B1 his (P2 eductant)	pFW2	"E. coli hisOGD in YIp5, amp, weak tet
FB120	ES942	via Colin Manoil		ES942.....	mutD5 metE lacZ trpA F-	none
FB1200	pDE24-8	D. Eisenmann	~4.4kb Hind3 frag located 3' to SPT15 gene (including CT of SPT15) cloned in to pRS316. (see sheet inserted with FB1196)	HB101.n...		pDE24-8	URA3 CEN ARS ampR
FB1201	pDE26-1	D. Eisenmann	2.4kb EcoRI-Bam piece containing SPT15 in YCp50. = original subclone of SPT 15 (see sheet inserted with FB1196)	HB101.n...		pDE26-1	SPT15 URA3 CEN ARS ampR
FB1202	pDE27-3	D. Eisenmann	Cla-Xba piece containing truncated SPT15 gene in pRS316 [derived by Xba dige stion of pDE18-1= FB1197] see sheet inserted with FB1196	HB101.n...		pDE27-3	URA3 CEN ARS ampR
FB1203	pDE69-1	D. Eisenmann	2.4kb RI-Bam piece containing spt15-21 in pRS316 (spt15-21 gene contains Sal I site upstream of the ATG put in by K. Arndt)	HB101.n...		pDE69-1	spt15-21 URA3 CEN ARS ampR
FB1204	p969-1	D. Eisenmann	original gap rescued plasmid containing spt15-122 in pRS316. pDE38-9 was us ed to rescue this allele from FW969.	HB101.n...		p969-1	spt15-122 URA3 CEN ARS ampR
FB1205	p971-5	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 from strain FW971 into pRS 316	HB101.n...		p971-5	spt15-135 URA3 CEN ARS ampR
FB1206	p1259-1	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 and strain FW1259 into pRS 316	HB101.n...		p1259-1	spt15-21 URA3 CEN ARS ampR
FB1207	pFM23-1	D. Eisenmann	"original plasmid from gapped rescue using pDE38-9 from FY183 (containing spt 15-23) into pRS316. Although this plasmid fails to complement 15-21 for h is+ phen., neither DME nor SLR could find a base change in the coding regi on. "	HB101.n...		pFM23-1	spt15-23 URA3 CEN ARS ampR
FB1208	pHCL17	D. Eisenmann	"HCS= high copy suppressor. = plasmid from YEp24 High Copy Library that suppr esses lys- phen. of spt15-21, 15-122, spt3d (strains become his+ lys+/-) see also FB 1209, 1210. "	HB101.n...		pHCL17	2 micron URA3 ampR HCS of spt15
FB1209	pHCL26	D. Eisenmann	"HCS= high copy suppressor. plasmid from YEp24 High Copy Library that suppre sses lys- phen. of spt15-21, 15-122 spt3d. (strains become his+ lys+/-) S ee also FB1208,1210. "	HB101.n...		pHCL26	2 micron URA3 ampR HCS of spt15
FB121	LE30	via Colin Manoil	mutD mutagenesis increased by TdR (usually enough in LB)	LE30......	mutD5 rpsL (strR) azi galU95 F-	none
FB1210	pHCL30	D. Eisenmann	"HCS= High Copy Suppressor. =plasmid from YEp24 High Copy Library that suppre sses lys- phen. of spt15-21, 15-122, spt3d (strains become his+ lys+/-) See also FB1208, 1209. "	HB101.n...		pHCL30	2 micron URA3 ampR HCS of spt15
FB1211	pSR7-1	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 from spt15-21 intragenic r evertant strain SR7 (into pRS316). AA change = F237V and G174E see also FB1212.	HB101.n...		pSR7-1	spt15-21R7 URA3 CEN ARS ampR
FB1212	pSR31-1	D. Eisenmann	original plasmid from gapped rescue using pDE38-9 from spt15-21 intragenic r evertant strain SR31 (into pRS316). AA change=F237V and G174E same as fo r FB1211's SR7.	HB101.n...		pSR31-1	spt15-21R31 URA3 CEN ARS ampR
FB1213	pSR47-1	D. Eisenmann	"original plasmid from gapped rescue using pDE38-9 from spt15-21 intragenic r evertant strain SR47 (into pRS316). AA change= F237V and G174E See also FB1211, 1212. "	HB101.n...		pSR47-1	spt15-21R47 URA3 CEN ARS ampR
FB1214	pDE79-3	D. Eisenmann	spt15-237 (aa change= F237V; intragenic rev) in wild type background. (done by 3way ligation) It complements 15-21 and has no spt- phen. whem shuffled over the null.	HB101.n...		pDE79-3	spt15-237 TRP1 CEN ARS ampR
FB1215	pDE103-1	D. Eisenmann	~2.5kb RI-BglII piece from pFW27 containing SPT3 into pUC118 RI-Bam.	HB101.n...		pDE103-1	SPT3 ampR
FB1216	pDE121-6	D. Eisenmann	SPT3 from plasmid pDE105-6 using primers ODE36 and ODE38. These put BamHI s ites at NT and CT of SPT3 open reading frame. Bam cut product was subclone d into pRS316 Bam.	HB101.n...		pDE121-6	SPT3 URA3 CEN ARS ampR
FB1217	pDE121-8	D. Eisenmann	see FB1216 for comments (pDE121-8 is a diff. isolate).	HB101.n...		pDE121-8	SPT3 CEN ARS URA3 ampR
FB1218	p3-401	D. Eisenmann	original plasmid from gapped rescue using pDE105-6 cut with MluI and XhoI in to spt15-21 revertant strain L805. p3-401 is in pMR79(=YCp50 with XhoI sit e knocked out)	HB101.n...		p3-401	spt3-401 URA3 CEN ARS ampR
FB1219	p3-445	D. Eisenmann	spt3-445 rescued from FY652 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-445	spt3-445 URA3 CEN ARS ampR
FB122		G. Walker	pKM101 derivative from Graham Walker	JC2926....	recA13 plus many others; look in strain notebook	pGW1514	"kanR, pKM101 derivative
FB1220	p3-1	D. Eisenmann	spt3-1 rescued from FW240 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-1	spt3-1 URA3 CEN ARS ampR
FB1221	p3-426	D. Eisenmann	spt3-426 rescued from FY648 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-426	spt3-426 URA3 CEN ARS ampR
FB1222	p3-429	D. Eisenmann	spt3-429 rescued from FY649 (into pMR79 =YCp50 with Xho site knocked out).	HB101.n...		p3-429	spt3-429 URA3 CEN ARS ampR
FB1223	p3-417	D. Eisenmann	spt3-417 rescued from FY647 into pMR79 (=YCp50 with Xho site knocked out).	HB101.....		p3-417	spt3-417 URA3 CEN ARS ampR
FB1224	p3-441	D. Eisenmann	spt3-441 rescued from FY650 into pMR79 (=YCp50 with Xho site knocked out).	HB101.....		p3-441	spt3-441 URA3 CEN ARS ampR
FB1225	p3-443	D. Eisenmann	spt3-443 rescued from FY651 into pMR79 (=YCp50 with Xho site knocked out).	HB101.....		p3-443	spt3-443 URA3 CEN ARS ampR
FB1226	pDE124-1	D. Eisenmann	~2.3kb RI-Xho frag from FB1218 (p3-401) into RI-Xho of pRS306 (for integrat ion of spt3-401).	HB101.....		pDE124-1	spt3-401 URA3 ampR
FB1227	pDE126-1	D. Eisenmann	~2.3kb RI-Xho frag containing spt3-429 from FB1222 (p3-429) into pRS306 RI-X ho (for integration of spt3-429)	HB101.....		pDE126-1	spt3-429 URA3 ampR
FB1228	pDE128-1	D. Eisenmann	~2.3kb RI-Xho frag containing spt3-445 from FB1219 (p3-445) into RI-Xho of p RS306. (for integration of spt3-445)	HB101.....		pDE128-1	spt3-445 URA3 ampR
FB1229	pDE132-23	D. Eisenmann	SPT3 with Bam ends derived by PCR (see FB1216) cloned into pMA424 to create an in frame fusion of GAL4 1-147 to all of SPT3. (has SPT3+ activity)	HB101.....		pDE132-23	GAL4-SPT3 fusion HIS3 2micron ampR
FB123		Winston	partial evictant of lys2-173R2	HB101.....		pFW48	"amp, part of lys2-173R2
FB1230	pMG1	M. Gandhi .	SalI-SpeI frag of SPT8 cloned into pRS316.	TB1.1.....		pMG1	SPT8 URA3 CEN ARS ampR
FB1231	pGAD10	S. Fields	see enclosed sheet.	HB101.....		pGAD10	GAL4 LEU2 ampR
FB1232	pKA67	Ricupero/Arndt	RI-Bam frag containing spt15-122 from pKA21 subcloned into RI-Bam of pRS316. (SalI site is present at spt15-122 ATG)	HB101.....		pKA67	spt15-122 URA3 CEN ARS ampR
FB1233	pKA68	Ricupero/Arndt	~2.4kb RI-Bam frag containing spt15-301 from pKA14 subcloned into RI-Bam of pRS316. (SalI site is present at spt15-301 ATG)	HB101.....		pKA68	spt15-301 URA3 CEN ARS ampR
FB1234		Nasmyth	2micron LEU2 library in YEp13 (2 vials frozen)	unknown...		2micron LEU2 library	2micron LEU2 library
FB1235	pKA71	S. Ricupero	HA mutagenized spt15-3 (FB998) to get intragenic suppressors of spt15-122 (s elected Lys+). This isolate= #13a A.A. change= P191S (CCT to TCT) + L205F (15-122) see also FB1236-1238.	HB101.n...		pKA71	spt15-319 ampR CEN ARS TRP1
FB1236	pKA72	S. Ricupero	pKA71= intragenic sup candidate #13b. SEE FB1235	HB101.n...		pKA72	spt15-319 ampR CEN ARS TRP1
FB1237	pKA73	S. Ricupero	PKA73= intragenic sup candidate #19. SEE FB1235	HB101.n...		pKA73	spt15-319 ampR CEN ARS TRP1
FB1238	pKA74	S. Ricupero	pKA74= intragenic sup candidate# 33. SEE FB1235	HB101.n...		pKA74	spt15-319 ampR CEN ARS TRP1
FB1239	Struhl#216	Ricupero .	mutant TATA seq= TATAAC. REF= MCB dec89 p.5298-5304 Harbury and Struhl **S EE ALSO FB1174-1190)	HB101.n...		p216	URA3 ampR integrating plasmid his3-216
FB124			FB124-FB152 all derivatives of pFW32	HB101.....		pFW32-302	spt3-302
FB1240	pCC10	Christy	~6kb XhoI-Hind3 frag from pCC27 into SalI-Hind3 sites of YCp50.	HB101.n...		pCC10	spt6 subclone ampR URA3 CEN ARS
FB1241	pCC19	Christy	Pvu-Sal 3.8kb frag containing SPT6 from pCC11 subcloned into YCp50 Pvu-Sal.	HB101.n...		pCC19	spt6 subclone URA3 CEN ARS ampR
FB1242	pCC32	Christy	Hind3-Hind3 spt6 subclone (~1.5kb) cloned into YCp50	HB101.n...		pCC32	spt6 subclone URA3 CEN ARS ampR
FB1243	pGEX-1	Glutagene (Cepko lab)	see sheet inserted in notebook.	HB101.n...		pGEX-1	for fusions with glutathione-S-tranferase
FB1244	pGEX-2T	Glutagene (Cepko lab)	see sheet inserted in notebook.	HB101.n...		pGEX-2T	for glutathione-S-transferase fusions
FB1245	pGEX-3X	Glutagene (Cepko lab)	see sheet inserted in notebook.	HB101.n...		pGEX-3X	for glutathione-S-tranferase fusions
FB1246	pPYKc/g	R. Kornberg	see sheet inserted in notebook.	HB101.n...		pPYKc/g	PYKI promoter ampR
FB1247	pCC60	Chris Clark	5.7kb SalI frag from HTA1-HTB1 cloned into the SalI site of YCp50	HB101.....		pCC60	HTA1-HTB1 URA3 tetS ampR
FB1248	pCC72	Chris Clark	1.4kb HTA1-HTB1 Hind3 frag was cloned into hind3 sites of pUC13-d16b (FB584= arsd of HTA1-HTB1) which has lost the 1.1kb H3 promoter frag.	HB101.....		pCC72	HTA1-HTB1d ampR
FB1249	pJH20	Hirschhorn	1.8kb StyI frag of Yip5 TRT2 into StyI site of pBR322	HB101.....		pJH20	HTA2-HTB2 truncated ampR TcR
FB125				HB101.....		pFW32-306	spt3-306
FB1250	pJH21	Hirschhorn	TRP1+ Bam-Bgl frag from pBM453 (FB451) into BglII of pJH20 (FB1249)	HB101.....		pJH21	(hta2-htb2)d::TRP1 ampR TcR
FB1251	pJH23	Hirschhorn	Bam-SacII 2.6kb frag from pCC60 (FB1247) into Bam- SacII of pRS313	unknown...		pJH23	HTA1-HTB1 HIS3 CEN ampR
FB1252	pJH24	Hirschhorn	RI-Bam HTA2-HTB2 frag from Yip5 into RI-Bam of pRS316	unknown...		pJH24	HTA2-HTB2 URA3 CEN ampR
FB1253	pJH28	Hirschhorn	1.1kb Hind3 frag from pRB58 (FB997) into Hind3 of pRS316	TB1.own...		pJH28	SUC2 promoter URA3 CEN ampR
FB1254	pJH29	Hirschhorn	~160bp RI-Hind3 frag from pJH28 (FB1253) --upstream of SUC2-- into RI-Hind3 sites of pUC19	TB1.own...		pJH29	SUC2 upstream ampR
FB1255	pJH26	Hirschhorn	Bam-SalI frag from pJH24 (FB1252) into Bam-SalI of pRS424	TB1.......		pJH26	HTA2-HTB2 TRP1 2micron ampR
FB1256	pJH31	Hirschhorn	Bam-SalI frag fron pJH24 (FB1252) into Bam-SalI of pRS425	TB1.......		pJH31	HTA2-HTB2 LEU2 2micron ampR
FB1257	pJH35	Hirschhorn	H3-H3 frag from pJH28 (FB1253) into H3 of pUC19	TB1.......		pJH35	SUC2 promoter ampR
FB1258	pJH38	Hirschhorn	pJH35 (FB1257) MluI NcoI filled in and YEp24 H3 (URA3 frag) filled in	FB600.....	pyrf	pJH38	suc2-101::URA3 ampR
FB1259	pJH42	Hirschhorn	1. RI-H3 from pJH28 (FB1253) into RI-H3 of mp19. 2. dut ung mut. with S2Tk (TATAAA to GGTACC). 3. RI-H3 frag into RI-H3 of pUC19.	TB1.0.....		pJH42	suc2-104 ampR
FB126				HB101.....		pFW32-307	spt3-307
FB1260	RZ1032	B. Berg	for dut ung mutagenesis	RZ1032....	dut1 ung1 thi1 relA HfrKL16 PO/45 [lysA61-62] 2bd-279::Th10 supE44
FB1261	pCT1	Rick Young	*DO NOT GIVE OUT!!*	DH5alpha..		pCT1	srb2-1 URA3 CEN ARS ampR
FB1262	pCT19	Rick Young	*DO NOT GIVE OUT!!*	DH5alpha..		pCT19	SRB2 URA3 CEN ARS ampR
FB1263	BC9b	Brendan Cormack (Struhl)	*There is ANOTHER SmaI site in the pBR portion*	HB101.ha..		pBC9b	DED1 promoter GCN4 3'UT URA3 CEN ampR
FB1264	GN1561-1	Georges Natsoulis	MNase engineered so that coding region is flanked by BamHI and SalI sites-- then cloned into pUC19. *ONLY mature protein coding seq.--NO ATG!!* REF: Nature 352:632-5 for 5' Bam site.	HB101.ha..		pGN1561-1	Micrococcal Nuclease (staphylococcal Nuc.) ampR
FB1265	pCC27	Chris Clark	SPT6 in YCp50.	unknown...		pCC27	SPT6 URA3 CEN ARS ampR
FB1266	GAL1-cDNA	Tony Bretscher	GAL1-cDNA library REF: Genetics 132 (1992) p. 665-673	DH10B			GAL1-cDNA library
FB1267	771	Roger Brent	EcoRV-RsaI frag containing cyc1-512 into StuI site of pRB486 (see enclosed s heet)	HB101.n...		p771	cyc1-512 terminator ampR
FB1268	798	Roger Brent	EcoRV-RsaI containing CYC1 into StuI site of pRB486 (see enclosed sheet).	HB101.n...		p798	CYC1 terminator ampR
FB1269	pPK606	M. Grunstein	REF: CELL 55:27-39	MH1.......		pPK606	hhf2d(4-23) CEN URA3 ampR
FB127				HB101.....		pFW32-309	spt3-309
FB1270	pPK617	M. Grunstein	REF: CELL 55:27-39	MH1.......		pPK617	hhf2d(4-14) CEN URA3 ampR
FB1271	pPK618	M. Grunstein	REF: CELL 55:27-39	MH1.......		pPK618	hhf2d(4-19) CEN URA3 ampR
FB1272	pUK499	M. Grunstein	REF: EMBO 7:2211-2219	HB101.....		pUK499	HHF2 CEN URA3 ampR
FB1273	pUK192	M. Grunstein	REF: EMBO 11:3297-3306	HB101.....		pUK192	(hht1-hhf1)d::LEU2 ampR
FB1274	pUK431	M. Grunstein	REF: EMBO 11:3297-3306	HB101.....		pUK431	(hht2-hhf2)d::HIS3 ampR
FB1275	pTaq-DNAP	G. Hartzog	Taq Polymerase expression vector REF: Anal Biochem 191: 396-400	HB101.....		pTaq-DNAP	ampR Taq. Pol. vector
FB1276	pJH359	R. Young	REF: Nature 347: 493 contains 205bp MaeII DNA frag containing the INO1 U AS inserted at the XhoI site of UAS-less CYC1-lacZ fusion vector pJH304	unknown...		pJH359	CYC1 TATA-lacZ INO1UAS ampR 2u
FB1277	pLGSD5	R. Young	REF: Nature 347: 493 contains the GAL10 UAS inserted at the XhoI site to r eplace the CYC1 UAS in the CYC1-lacZ fusion vector pLGd2925	unknown		pLGSD5	CYC1 TATA-lacZ GAL10-UAS ampR 2u
FB1278	pHYC3	R. Young	REF: Nature 347: 493 contains HIS4 UAS inserted at the XhoI site to repla ce the CYC1 UAS in the CYC1-lacZ fusion vector pLG669-2	unknown...		pHYC3	CYC1 TATA-lacZ HIS4-UAS ampR 2u
FB1279	pCT3	R. Young	#NAME?	unknown...		pCT3	"URA3 ampR CEN ARS Bluescript KS polylinker T3,T7 promoters
FB128		.		DB6507....		pFW32-318	spt3-318
FB1280	pCT15	R. Young	=2.5kb frag containing SRB4 into ploylinker of pCT3 see FB1279	unknown...		pCT15	SRB4 URA3 CEN ARS ampR
FB1281	pCT48	R. Young	"2.5kb frag containing srb4-1 into pCT3 see also FB1280, FB1279 "	unknown...		pCT48	srb4-1 URA3 CEN ARS ampR
FB1282	pCT39	R. Young	1.9kb insert --- obtained by gap repair of SacI/XhoI of pCT32 (FB1283)	unknown...		pCT39	SRB5 URA3 CEN ARS ampR
FB1283	pCT32	R. Young	"see also FB1282, FB1279 "	unknown...		pCT32	srb5-1 URA3 CEN ARS ampR
FB1284	pCT40	R. Young	pCT40 (SRB6) obtained by gap repair of BalI/SphI in pCT29 (FB1285)	unknown...		pCT40	SRB6 URA3 CEN ARS ampR
FB1285	pCT29	R. Young	"see also FB1284, FB1279 "	unknown...		pCT29	srb6-1 URA3 CEN ARS ampR
FB1286	pAB24	Chiron Corp. (T. Brake)	*K. lactis library* REF: NAR vol.19 No.19: 5345-5350. See Yeast Library notebook	DH5.own...		pAB24	K. lactis library URA3 ampR
FB1287	pJH310	S. Henry .	"REF: Hirsch + Henry (1986) MCB 6, 3320-3328 see also FB1288 and sheets in n otebook. "	unknown		pJH310	INO1 ampR SP6 promoter
FB1288	pJH311	S. Henry .	"REF: Hirsch + Henry (1986) MCB 6, 3320-3328. See also FB1287 AND SHEETS IN NOTEBOOK "	Unknown		pJH311	INO1 ampR SP6 promoter
FB1289	RS426-TDS1	Buratowski.	TDS1 is a high copy suppressor of YEpGAL-IID[R105]. Vector= pRS426	unknown		pRS426-TDS1	TDS1 URA3 2micron ampR
FB129		.		DB6507....		pFW32-319	spt3-319
FB1290	pL909	B. Berg (U. Chicago)	REF: Apricio et al. CELL 66: 1279-1287 (1991) contains a 3.6kb ADE2 BamHI frag in YEp13.	unknown...		pL909	ADE2 ampR
FB1291	pdADE2	B. Berg (U. Chicago)	REF: Aparicio et al. CELL 66:1279-1287(1991) Alani et al. Genetics 116:541 (1987) =ADE2::hisG URA3 disruption in bluescript derivative pV21	unknown...		pdADE2	ADE2::hisGURA3 ampR
FB1292	pHB25	Henry Baker	= 7.4kb SalI frag of the GCR region into which the 3.5kb BclI frag was repla ced by 3.5kb BglII LEU2 frag (in pBR322)	unknown...		pHB25	gcr::LEU2 ampR
FB1293	pHB45	Henry Baker	BclI frag of GCR replaced with BamHI HIS3 frag into SalI of pBR322. See als o FB1292.	unknown...		pHB45	gcr::HIS3 ampR
FB1294	pLG36	L. Gansheroff	FB422 plasmid cut with XbaI and SacI; insert LEU2 on Xba/Sac frag cut out of FB942	HB101.n...		pLG36	ste12d::LEU2 URA3 ampR
FB1295	pBM290	Johnston .	"REF: Yocum + Johnston (1984) Gene 32,75-82. =GAL80 disruption plasmid. GAL 80 gene is NOT present on this plasmid. See enclosed sheets. "	unknown		pBM290	URA3 SUP11 GAL80 flanking sequences ampR
FB1296	pBM1974	Johnston .	"REF; Griggs + Johnston (1993) MCB 13, 4999-5009 See enclosed sheets. "	unknown		pBM1974	GAL4-CAT fusion URA3 ampR
FB1297	pmlc28ARG4	Szostak via C. Epstein	ARG4 3.2kbp Pst frag in pmlc28 See enclosed sheets	MC1061....		pmlc28Pst6ARG4	ARG4 CmR
FB1298	pmlc12ARG4	Szostak via C. Epstein	Full plasmid name= pmlc12PstARG4BglIIFill (BamHI URA3) BglII site in ARG4 mutated to create ClaI site (in pmlc12) see enclosed sheets	MC1061....		pmlc12PstARG4BglII	arg4 URA3 CmR
FB1299	pACR1	Roger Brent		DH5 (?)..		pACR1	ACR1 ampR URA3
FB13	B44/pFW3	Winston	YIp5 with the HindIII site destroyed by filling in with reverse transcriptas e	DB6507....	HB101 pyrF74::Tn5	pFW3	URA3 amp
FB130		.		DB6507....		pFW32-322	spt3-322
FB1300	pdACR1	Roger Brent		DH5 (?)		pdACR1	acr1::URA3 ampR LEU2
FB1301	LR1d1	Roger Brent	GAL1 deleted UAS leaves XhoI site. NO LexA ops. this strain is the negati ve control for FB1302-FB1306.	DH5 (?).		LR1d1	GAL1dUAS-lacZ URA3 ampR 2micron
FB1302	pSH18-18	Roger Brent	"see also FB1301, FB1303-1306. "	DH5 (?)..		pSH18-18	GAL1dUAS-lacZ URA3 2u ampR LexA ops. (6)
FB1303	pRB1840	Roger Brent	"see also FB1301-1302, FB1304-1306 "	DH5 (?)..		pRB1840	GAL1dUAS-lacZ URA3 2u ampR LexA op.
FB1304	pSH18-34	Roger Brent	"see also FB1301-1303, FB1305-1306 "	DH5 (?)..		pSH18-34	GAL1dUAS-lacZ URA3 2u ampR LexA ops (8)
FB1305	pSH18-8	Roger Brent	"see also FB1301-1304, FB1306 "	DH5 (?)..		pSH18-8	GAL1dUAS-lacZ URA3 ampR 2u LexA ops. (4)
FB1306	SH1834dspe	Roger Brent	see also FB1301-1305	DH5 (?)..		pSH18-34dspe	GAL1dUAS-lacZ URA3 ampR integrating LexA ops.
FB1307	pSH17-4	Roger Brent		unknown...		pSH17-4	HIS3 LexA-GAL4 ampR 2u
FB1308	pCY40	Tom Stevens	REF: MCB 10:3737 Use 4.3kb HindIII frag for disruption.	MC1066....		pCY40	vat2d1::LEU2 ampR
FB1309	pGH2	Grant Hartzog		MC1061....		pGH2	TRP1 ARS1 ampR (in SP72)
FB131		Winston		DB6507....		pFW32-326	spt3-326
FB1310	pGH4	Grant Hartzog		MC1061....		pGH4	SPT6 CEN ARS URA3 ampR
FB1311	pGH5	Grant Hartzog	3.33kb Bam-H3 frag from FB1087 into Bam-H3 cut pRS426	MH1.61....		pGH5	GAL4 ampR URA3 2u
FB1312	pGH6	Grant Hartzog	3.33kb Bam-H3 frag containing GAL4 into Bam-H3 cut pRS425	MH1.61....		pGH6	GAL4 ampR LEU2 2u
FB1313	pGH7	Grant Hartzog		MC1061....		pGH7	SPT5 HIS3 ADH promoter ampR 2u
FB1314	pGH8	Grant Hartzog		MH1.61....		pGH8	SPT5 TRP1 ampR 2u GAL1 promoter
FB1315	pGH9	Grant Hartzog	"see also FB1313, FB1314 "	MH1.61....		pGH9	SPT5 coding ampR
FB1316	pJK101	Roger Brent	LacZ reporter with upstream UAS and intervening lexA operator. For Blockin g Assay. *DO NOT DISTRIBUTE!!*	MC1061		pJK101	URA3 ampR GAL1-lacZ LexA binding downst. UAS
FB1317	pJG4-4	Roger Brent	= version of pJG4-5 (FB1319) that lacks ATG-Nuc. loc. epitope tag-acidic act ivator. For two hybrid system. *DO NOT DISTRIBUTE!!*	MH1		pJG4-4	TRP1 ampR 2u ori Gal1 promoter ADH terminator
FB1318	pJG4-6	Roger Brent	= version of pJG4-5 (FB1319) that has ATG-epitope tag but lacks the nuclear localization signal and acidic activation domain. For two hybrid system. *DO NOT DISTRIBUTE!!*	MH1		pJG4-6	ampR TRP1 2u ori Gal1 promoter ADH terminator
FB1319	pJG4-5	Roger Brent	See enclosed sheets for more info. *DO NOT DISTRIBUTE!!*	MC1061		pJG4-5	ampR TRP1 2u ori Gal1 promoter ADH terminator
FB132		Winston		DB6507....		pFW32-327	spt3-327
FB1320	pJG4-5s	Roger Brent	See enclosed sheets for more info *DO NOT DISTRIBUTE!!*	MH1		pJG4-5s	ampR TRP1 2u ori Gal1 promoter ADH terminator
FB1321	pRFHM1	Roger Brent	REF: Nature 350:250 *DO NOT DISTRIBUTE!!*	MC1061....		pRFHM1	HIS3 ampR 2u deriv LexA 202+PL
FB1322	pEG202	Roger Brent	= deriv. of LexA202+Pl see enclosed sheets for more info *DO NOT DISTRIB UTE!!*	MC1061		pEG202	HIS3 2u ampR LexA
FB1323	DH10/P3	Grant Hartzog	E. coli strain for propagating supF plasmids	DH10/P3...	"F- mcrA d(mrr-hsd RMS-mcrBC) 0 80d lacZdM15 dlacX74 deoR recA1 arad139 a(ara, leu)7697 galu galk
FB1324	pSR1-2	Shannon Roberts	7.3kb Cla-RI frag from pFW127 inserted into Cla-RI of pRS404	DH5 alpha.		pSR1-2	SPT7 ampR TRP1
FB1325	pSR2-1	Shannon Roberts	3.1kb Sal-Spe frag cont. SPT8 from pFW120 inserted in Sal-Spe polylinker of pRS405	MH1.alpha.		pSR2-1	SPT8 LEU2 ampR
FB1326	pSR3	Shannon Roberts	#NAME?	DH5 alpha.		pSR3	URA3 2u ampR
FB1327	pSR4-5	Shannon Roberts	constructed by cutting out 3kb Sal-Spe SPT8 frag from pMG1 and inserting it into Sal-Spe of pSR3 (FB1326) *for epitope tagging SPT8*	DH5 alpha.		pSR4-5	SPT8 URA3 2u ampR
FB1328	pSR5	Shannon Roberts	3kb SPT8 Sal-Spe frag into Sal-Spe of pRS426	DH5 alpha.		pSR5	SPT8 URA3 2u ampR
FB1329	pSR6-3	Shannon Roberts	SPT8 tagged at N term.	DH5 alpha.		pSR6-3	SPT8-myc epitope URA3 ampR 2u
FB133		Winston		DB6507....		pFW32-328	spt3-328
FB1330	pSR7-3	Shannon Roberts	3.1kb Sal-Spe SPT8-N term myc epitope tagged prot. from pSR6-3 (FB1329) into Sal-Spe of pRS424	DH5 alpha.		pSR7-3	SPT8-myc epitope TRP1 ampR 2u
FB1331	EB191	Elaine Elion	STE12 tagged at C term.	HB101.pha.		pNC252	2u URA3 ampR GAL1 STE12-myc epitope
FB1332	pJS90	Marian Carlson	REF: MCB 10:4744	HB101.....		pJS90	ssn6d9URA3 ampR
FB1333	pLN113-3	Marian Carlson	REF: MCB 7:3637	HB101.....		pLN113-3	SSN6 2micron ampR URA3+
FB1334	pLexASNF2	Marian Carlson	REF: PNAS 88:2687	HB101.....		pLexASNF2	LexA-SNF2 2micron HIS3 ampR
FB1335	pLexASNF5	Marian Carlson	REF: MCB 10:5616	HB101.....		pLexASNF5	LexA-SNF5 2micron HIS3 ampR
FB1336	pLexASNF6	Marian Carlson	REF: Genes + Dev. 6:1707 NOTE: this is LexA 1-202 + SNF6 according to B. L aurent.	HB101.....		pLexASNF6	LexA-SNF6 2micron HIS3 ampR
FB1337	pGP59	Greg Prelich	= original BUR1+ clone from YCp50 Bam/Sau3A library. (Complements bur1-1 and bur1-2	HB101.....		pGP59	ampR URA3 CEN ARS BUR1
FB1338	pGP73	Greg Prelich	= original clone from YCp50 CEN library (Complements bur5-1)	HB101.....		pGP73	ampR URA3 CEN ARS copyII (HHT2-HHF2)
FB1339	pGP74	Greg Prelich	= an original bur5-1 complementing plasmid isolated from YCp50 CEN library.	HB101.....		pGP74	ampR CEN ARS URA3 BUR5 (copyI=HHT1-HHF1)
FB134		Winston		DB6507		pFW32-330	spt3-330
FB1340	pGP89	Greg Prelich	a 4.5kb EcoRI frag from pGP59 (FB1337) containing BUR1+ subcloned into EcoRI site of YIp5	HB101.....		pGP89	ampR tetR URA3 BUR1frag.
FB1341	pGP103	Greg Prelich	~2250bp ClaI frag from pGP91 containing BUR1+ was put into pRS316 ClaI vecto r (=partial BUR1 clone---complements bur1-)	HB101.....		pGP103	ampR CEN URA3 BUR1
FB1342	pGP105	Greg Prelich	a 1227bp TRP1 Bam/Bgl frag of YRp7 was put in place of the 170bp BglII frag in pGP103 (FB1341) to create a bur1d marked by TRP	TB1		pGP105	ampR CEN URA3 bur1d1::TRP1
FB1343	pGP147	Greg Prelich	a 6kb SphI frag of pGP74 (FB1339) containing BUR5 was subcloned into the Sph I site of YCp50 (Complements bur5-1)	TB1.1.....		pGP147	ampR CEN ARS URA3 HHT1-HHF1
FB1344	pGP150	Greg Prelich	a 3kb KpnI-PvuII frag from pGP147 (FB1343) was cloned into the KpnI-SmaI sit es of pRS416	TB1.1.....		pGP150	ampR CEN6 ARS1+4 URA3 HHT1
FB1345	pGP153	Greg Prelich	1.6kb SphI-ClaI frag from pGP147 (FB1343) was put into the SphI-ClaI sites o f YCp50.	TB1.1.....		pGP153	ampR CEN4 ARS1 URA3 HHT1-HHF1
FB1346	pGP154	Greg Prelich	a 1.2kb EagI-KpnI frag from pGP147 (FB1343) was put into the EagI-KpnI sites of pRS416.	TB1.1.....		pGP154	ampR CEN6 ARS URA3 HHF1
FB1347	pGP156	Greg Prelich	"a 5.4kb frag of pMS191 was put into the Bam-Hind3 sites of pRS305 to allow i ntegration at copyI, marked by LEU2, after SmaI linearization. "	TB1.1.....		pGP156	ampR LEU2 HHT1-HHF1
FB1348	pLG40	Lisa Gansheroff	E. coli is SICK! See notebook.	HB101.....		pLG40	"his4-912d::URA3, lacZ ampR
FB1349	pLG41	Lisa Gansheroff	E. coli is SICK! See notebook.	HB101.....		pLG41	"his4-912d::URA3, lacZ ampR
FB135		Winston		DB6507....		pFW32-332	spt3-332
FB1350	pLG39	Lisa Gansheroff	E. coli is SICK! See notebook.	HB101.....		pLG39	Ty912d44-lacZ fusion frag URA3 ampR
FB1351	pLG56	Lisa Gansheroff	E. coli is SICK! See notebook.	HB101.....		pLG56	Ty912d44-lacZ fusion frag URA3 ampR
FB1352	pSAB3	Steve Brown	SalI-SalI frag containing HTA1-HTB1 (5.7kb) into pRS316	TB1.1.....		pSAB3	URA3 ampR CEN ARS HTA1-HTB1
FB1353	pSB17	Steve Brown	= a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-46: dhH=G46D) See enclosed sheets in notebook.	HB101.....		pSB17	URA3 ampR CEN ARS HTA1-46
FB1354	pSB6	Steve Brown	= a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-48: ddhA=G48D) se e enclosed sheets in notebook.	HB101.....		pSB6	URA3 ampR CEN ARS HTA1-48
FB1355	pSB23	Steve Brown	=a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-61: dhV=A61T) see e nclosed sheets in notebook.	HB101.....		pSB23	URA3 CEN ARS ampR HTA1-61
FB1356	pSB7	Steve Brown	=a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-90: ddhC=R90I) se e enclosed sheets in notebook.	HB101.....		pSB7	URA3 CEN ARS ampR HTA1-90
FB1357	pSB19	Steve Brown	=a derivative of pSAB3 (FB1352) mutagenized by HA (HTA1-107: dhN=G107D) s ee enclosed sheets in notebook.	HB101.....		pSB19	URA3 CEN ARS ampR HTA1-107
FB1358	pSB9	Steve Brown	=a derivative of pSAB3 (FB1352) mutagenized by HA (HTB1-105: ddHE=A105D) see enclosed sheets in notebook.	HB101.....		pSB9	URA3 CEN ARS ampR HTB1-105
FB1359	XL1-Blue	Stratagene	can use for M13 helper phage preps. See enclosed sheets in notebook.	XL1-Blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F` proAB lacIq ZdM15 tn10 tet-]
FB136		Winston		DB6507....		pFW32-334	spt3-334
FB1360	pJL202	Schiestl .b	replaces URA3 with a 1.1kb copy of HIS3 (complete deletion of URA3)	MH1.Blue..		pJL202	ura3::HIS3 ampR
FB1361	pREP3X	Enoch	#NAME?	MH1.Blue..		pREP3X	nmt-1 promoter LEU2 ampR
FB1362	pM1329	Stillman	1.2kb PstI-RI subclone of CTS1 into pUC13 *Northern Probe* see sheets in notebook.	unknown...		pM1329	CTS1 ampR
FB1363	pM1591	Stillman	contains 1.3kb upsteam of CTS1. See enclosed sheets.	unknown...		pM1591	CTS1-lacZ URA3 ampR
FB1364	pHIR1::URA	Osley	1.4 BglII frag of HIR1 replaced with 1.1kb URA3 frag into YIp5. REF: Sherwo od et al MCB 1993 vol.13 no.1 p.28-38.	HB101.n...		pHIR1::URA3	HIR1::URA3 ampR URA3
FB1365	pHIR1::LEU	Osley	1.4kb BglII frag of HIR1 replaced with 3kb LEU2 BglII frag REF: Sherwood e t al MCB 1993 vol.13 no.1 p.28-38.	HB101.n...		pHIR1::LEU2	HIR1::LEU2 ampR URA3
FB1366	pGEM150-	Howley lab	Bam-H3 frag of E6-AP in pGEM1 REF: Huibreqtse et al MCB 13:775-784 1993	HB101.n...		pGEM150-120	E6-AP ampR
FB1367	pFW272	Stephanie Ricupero	Bam-H3 frag containing E6-AP from FB1366 cloned into BglII-H3 of FB1080 (pDA D2)	HB101.n...		pFW272	ampR URA3 2micron E6-AP GAL10 promoter
FB1368	pRB1027	Roger Brent	REF: Cell 43:729-736	HB101.n...		pRB1027	PADH1-LexA-GAL4 fusion 2micron LEU2 ampR
FB1369	pAAH5	Roger Brent		HB101.n...		pAAH5	PADH1vector 2micron LEU2 ampR
FB137		Winston		DB6507....		pFW32-335	spt3-335
FB1370	p1107	Roger Brent	derived from pLG670Z (FB1373)	HB101.n...		p1107	LexAop CYC1-TATA-LacZ fusion URA3 ampR 2micron
FB1371	pRB1345	Roger Brent	vector= pAAH5 (FB1369)	HB101.n...		pRB1345	PADH1-LexA-GCN4 fusion 2micron ampR LEU2
FB1372	pJLb-17b3	Roger Brent		HB101.n...		pJLb-17b3	GAL4 binding site CYC1-TATA-LacZ URA3 2micron ampR
FB1373	pLG670Z	Roger Brent	* NO UAS * REF: PNAS 78 2199-2203 (1981)	HB101.n...		pLG670Z	CYC1-TATA-LacZ URA3 ampR 2micron
FB1374	Bd1128	G. Mikesell	= pGAL::HIS3 in YCp50 REF: Genes + Dev. 5:1183-1190	HB101.n...		Bd1128	pGAL::HIS3 ampR CEN ARS URA3
FB1375	Bd824	Mikesell	=pGAL::SWI4 in YCp50 REF: Genes and Dev. 5:1183-1190	HB101.....		Bd824	pGAL::SWI4 URA3 CEN ARS ampR
FB1376	pSR9.15	S. Roberts		MH1.1.....		pSR9.15	SPT8 CEN URA3 ampR
FB1377	pBBB2	Barbara Berg	=NdeI-BamHI frag containing SPT3 from pDE118-8 into NdeI-BamHI of pET-3C (FB 894) SEE SHEETS IN NOTEBOOK.	BL21(DE3).	see enclosed sheets for info on host. Bl21(DE3) also contains pLysS w hich is CmR.	pBBB2	T7 promoter ampR SPT3 CmR
FB1378	pBBB9	Barbara Berg	PvuII-SalI frag containing rsp5 from pFW201 subcloned into pRS316	VJS533.3).	#NAME?	pBBB9	ampR CEN ARS URA3 rsp5 PvuII-SalI frag
FB1379	pBBB10	Barbara Berg	BamHI-HindIII frag containing rsp5 from pFW201 subcloned into pRS316	VJS533.3).	#NAME?	pBBB10	ampR CEN ARS URA3 rsp5 BamHI-HindIII frag
FB138		Winston		DB6507....		pFW32-336	spt3-336
FB1380	pBBB11	Barbara Berg	BamHI-BamHI frag containing rsp5 from pFW201 subcloned into pRS316	VJS533.3).	#NAME?	pBBB11	ampR CEN ARS URA3 rsp5 BamHI-BamHI frag
FB1381	pBBB12	Barbara Berg	SalI-HindIII frag containing rsp5 from pFW201 subcloned into pRS316. *NOT E: construct may be incorrect*	VJS533.3).	#NAME?	pBBB12	ampR CEN ARS URA3 rsp5 SalI-HindIII frag
FB1382	pBBB13	Barbara Berg	KpnI-HindIII frag containing rsp5 from pFW201 subcloned into pRS316. *NOT E: constrcuct may be incorrect!*	VJS533.3).	#NAME?	pBBB13	ampR CEN ARS URA3 rsp5 KpnI-HindIII frag
FB1383	pBBB14	Barbara Berg	BamHI-SalI frag containing rsp5 from pFW201 subcloned into pRS316	VJS533.3).	#NAME?	pBBB14	ampR CEN ARS URA3 rsp5 BamHI-SalI frag
FB1384	pSH2-1	Brent lab	REF: Cell 51:113; 1987 Cell 43:729; 1985 LexA polylinker vector	unknown.).		pSH2-1	ADH1promoter-lexA ampR 2micron HIS3
FB1385	KB125	Ricupero/Arndt	~1.9kb SpeI frag containing 2micron origin was excised from p1107 (FB1370) a nd plasmid was religated.	HB101.....		pKA113	LexA Op CYC1-TATA-lacZ URA3 ampR integrating
FB1386	pAS1-CHY2	Steve Ellidge	for 2 hybrid experiments	MH1.1.....		pAS1-CHY2	ampR 2micron CYH2 TRP1 ADHp-GAL4-HAI fusion
FB1387	YEpCHY1	Elion lab plasmid	~5.4kb of cyh2R gene in YEp24 cycloheximide resistant	MH1.1.....		YEpCHY1	URA3 ampR 2micron cyh2R
FB1388	pSR10-1	Shannon Roberts	NOTE: used for interaction trap. See enclosed sheets.	MH1.1.....		pSR10-1	ampR HIS3 2micron LexA(1-202)-SPT8(35-602)
FB1389	pSR11-8	Shannon Roberts	NOTE: used for interaction trap. See enclosed sheets.	MH1.1.....		pSR11-8	ampR TRP1 2micron B42-SPT8(35-602)
FB139		Winston		DB6507....		pFW32-339	spt3-339
FB1390	pJM124myc	Eun-Chung Park	REF: Embo vol.11 No.6 p.2087-2093 (1992)	unknown...		pJM124myc	NAT-myc LEU2 ampR 2micron
FB1391	ARDmyc	Eun-Chung Park	REF: Embo Vol.11 No.6 p.2087-2093 (1992)	unknown...		YEp24ARDmyc	URA3 ARDmyc ampR 2micron
FB1392	pmcm1-1	M. Harris	3.3kb SphI-BamHI frag containing mcm1-1 inserted into Yip5.	HB101.n...		pmcm1-1	mcm1-1 ampR URA3
FB1393	pJH662	Jim Haber	see sheets.	BJH5..n...		pJH662	ampR MATalpha tsm1::LEU2
FB1394	pJH569	Jim Haber	see sheets.	BJH2..n...		pJH569	URA3 ampR TSM1 MATa inc.
FB1395	pSV14	Roger Brent	GAL4 17mer is 178bp upstream of CYC1 TATA	HB101.n...		pSV14	GAL4 17mer CYC1-TATA-lacZ fusion URA3 ampR 2micron
FB1396	pRR330	Louis Prakash	=plasmid for RAD9 knockout. REF: MCB 1989 Vol.9 No.5 p.1882	unknown...		pRR330	RAD9::URA3 ampR
FB1397	pGN1262	Jef Boeke	for 1-step deletion-insertion of SPT21 (cut with XhoI and transform)	XL-Blue...		pGN1262	URA3 ampR SPT21
FB1398	pLG72	Lisa Gansheroff	insert= HindIII frag from pDBE6 containing unstable derivative of U6 gene v ector= pRS306 cut with HindIII *DO NOT GIVE OUT--SEE SHEET!*	HB101.e...		pLG72	U6 unstable derivative ampR URA3
FB1399	pLG73	Lisa Gansheroff	same construct as pLG72 (FB1398) but insert is in opposite direction. *DO NOT GIVE OUT--SEE SHEET!*	HB101.e...		pLG73	U6 unstable derivative ampR URA3
FB14	B44/pFW4	Winston	SalI site destroyed by filling in with reverse transcriptase	DB6507....	HB101 pyrF74::Tn5	pFW4	"URA3, amp
FB140		Winston		DB6507....		pFW32-343	spt3-343
FB1400	pLG74	Lisa Gansheroff	insert= HindIII frag from pDBE6 containing an unstable (mRNA) derivative of U6 gene. vector= pRS316 cut with HindIII **DO NOT GIVE OUT--SEE SHEET! **	HB101.e...		pLG74	U6 unstable derivative ampR URA3 CEN ARS
FB1401	pLG75	Lisa Gansheroff	same construct as pLG74 (FB1400) but insert is in the opposite orientation. *DO NOT GIVE OUT--SEE SHEET!*	HB101.e...		pLG75	U6 unstable derivative ampR URA3 CEN ARS
FB1402	pLG76	Lisa Gansheroff	insert= EagI-SalI frag from FB1148 containing N terminal half of SPT7 vect or= plasmid FB374 (C term. half of SPT7 in YCp50) cut with EagI and SalI.	HB101.e...		pLG76	SPT7 CEN URA3 ampR
FB1403	pLG77	Lisa Gansheroff	insert= EagI-SalI frag from FB1149 containing N term. half of SPT7 with brom odomain deleted vector= plasmid of FB374 (C term. half of SPT7 in YCp50) cut with EagI and SalI.	HB101.e...		pLG77	spt7 bromodomain deletion CEN URA3 ampR
FB1404	pLG78	Lisa Gansheroff	insert= EagI-SalI frag from FB1148 containing N term. half of SPT7 vector = plasmid of FB384 (C term. half of SPT7 in YIp5) cut with EagI and SalI	HB101.e...		pLG78	ampR URA3 SPT7
FB1405	pLG79	Lisa Gansheroff	insert= EagI-SalI frag from FB1149 containing N term. half of SPT7 with Brom odomain deleted vector= plasmid of FB384 (C term. half of SPT7 in YIp5) cut with EagI and SalI	HB101.e...		pLG79	spt7 bromodomain deletion ampR URA3
FB1406	D16pUC13+	MaryAnn Osley	=deletion 16prime (D16prime) with a XhoI site *SalI site is gone* =TRT 1 promoter with negative element deleted (region is marked by URA3+ insert ed downstream of H2B	HB101.e...		D16pUC13+	D16prime ampR
FB1407	pMS18	Michelle Swanson	pRS316 + 3.2kb NruI frag from pMS4 containing SPT5. REF: Swanson et al MCB vol.11 no.6 June 91 p.3009-3019.NOT FUNCTIONAL COPY OF SPT5	unknown...		pMS18	URA3 ampR CEN ARS SPT5
FB1408	pMS37	Michelle Swanson	YCp50 cut HindIII and NruI + pMS4 HindIII-PvuII frag. REF: Swanson et al M CB vol.11 no.6 June 91 p.3009-3019 NOT A FUNCTIONAL COPY OF SPT5	unknown...		pMS37	URA3 ampR SPT5
FB1409	MB81	Michelle Swanson	2.6kb SalI frag containing SacB cloned into SalI site of pSELECT (now known as pALTER) Strain is Amp SENSITIVE!	JM109.n...		pMB81	SacB ori TetR ampS SP6 T7promoter
FB141		Winston		DB6507....		pFW32-343	spt3-343
FB1410	MB88	Michelle Swanson	= in vitro mutagenesis of PMB81 (FB1409).	JM109.n...		pMB88	SacB-BamHI mutant ampR TetR ori Sp6 T7promoter
FB1411	pRS12	Louis Prakash	REF: MCB 1989 vol.9 no.5 p.1882-1896	unknown...		pRS12	RAD9 2micron LEU2 ampR