Winston Lab Strain Finder
Department of Genetics

Bacteria


Displaying 1 - 500 of 2807
Contains
Strain Name Strain Alias Source Comments Host Host Markers Plasmids Plasmid Markers
Joel
hml
%hml%
FB2491 pGN965 pGN965
FB2491 pGN965 Jeff Boeke Ref: The New Biologist V.3 No.12 p1249-1259 (1991)
YEp13 		pGN965 ura3-153 LEU2 2micron
FB2493 pET28B Rolf Sternglanz for protein expression Novagen pET28B Kanamycin
pNOY436
phz1
pms4
pms4
FB2516
pMS37
FB2561 pHM27 Alain Verreault YcP22 pHM27 HHT1-HHF1 CEN TRP1
FB2562 pHM146 Alain Verreault YCp22 pHM146 hht1K56A-HHF1 CEN TRP1
FB2563 pHM147 Alain Verreault YCp22 pHM147 hht1K56Q-HHF1 CEN TRP1
FB2564 pHM148 Alain Verreault YCp22 pHM148 hht1K56R-HHF1 CEN TRP1
FB2565 pHM169 Hiroshi Masumoto YIplac204, cut with Bsu36I to integrate
will not work in a trp1D63 strain 		pHM169 HHT1-HHF1 TRP1
FB2566 pHM164 Alain Verreault YIplac204 TRP1 cut with Bsu36I to integrate, but doesn't work with trp1D63 pHM164 hht1K56A-HHF1 TRP1
FB2567 pHM165 Alain Verreault YIplac204 TRP1 cut with Bsu36I to integrate but doesn't work for trp1d63 pHM165 hht1K56Q-HHF1 TRP1
FB2568 pHM166 Alain Verreault YIplac204 TRP1 cut with Bsu36I to integrate but doesn't work for trp1D63 pHM166 hht1K56R-HHF1 TRP1
FB2569 CB107 Charlie Hoffman self-ligated Invitrogen pNMT1-TOPO vector
full strength nmt1 promoter 		pNMT1-TOPO nmt1 promoter S.c. LEU2
FB2570 CB109 Charlie Hoffman self ligated Invitrogen pNMT41-TOPO vector mid strength nmt1 promoter pNMT41-TOPO nmt41 promoter S.C. LEU2
FB2571 CB111 Charlie Hoffman self ligated Invitrogen vector pNMT81-TOPO low strength nmt1 promoter pNMT81-TOPO nmt81 promoter S.c. LEU2
psh17-4
FB2576 p433 Angelica Amon AF mutation introduces a BsmI site in cdc28 p433 cdc28-T18A Y19F URA3
FB2114
%FB2114%
fb2044
dh5a
dh5alpha
fb843
fb843
dh5a
pLBS27
pLBS27
pMRFW2
fb603
FB2694 Yeast 2009; 26: 185-192 pFA6-6xGLY-FLAG-KanMX 6xGLY-FLAG-KanMX
FB2695 Yeast 2009; 26: 185-192 pFA6-6xGLY-3xFLAG-KanMX 6xGLY-3xFLAG-KanMX
FB2696 Yeast 2009; 26: 185-192 pFA6-6xGLY-Myc-KanMX 6xGLY-Myc-KanMX
FB2687 pZM467 Struhl Lab Struhl Lab. Yeast 2008: Volume 25; 287-292. 3xFLAG URA3 3xFLAG
FB2688 pZM473 Struhl Lab Struhl Lab. Yeast 2008: Volume 25; 287-292. 3xHSV URA3 3xHSV
FB2689 pZM474 Struhl Lab Struhl Lab. Yeast 2008: Volume 25; 287-292. 3xV5 URA3 3xV5
FB2690 pZM475 Struhl Lab Struhl Lab. Yeast 2008: Volume 25; 287-292. VSV-G URA3 VSV-G
FB
FB
fb
FB2702 pLP891 SIR2 HIS3 pLP891
FB2703 pLP1047 SIR3 HIS3 pLP1047
FB2704 pLP2206 SIR4 HIS3 pLP2206
FB2705 pPK128 HTA1-HTB1 HHT1-HHF1 LEU2 pPK128
FB2702 Jenny Chang pLP891 SIR2 HIS3
FB2703 Jenny Chang 2u pLP1047 SIR3 HIS3
FB2704 Jenny Chang pLP2206 SIR4 HIS3
FB2705 Jenny Chang pPK128 HTA1-HTB1 HHT1-HHF1 LEU2
FB2712 pLP2018 Lauren Clark, Pillus Lab pLP2018 AmpR NAB3 URA3
FB2713 pLP2054 Lauren Clark, Pillus Lab pLP2054 AmpR NRD1 URA3
FB2718 Zx03 Haber Lab Zx03 AmpR URA3 GAL-PMA1
FB2719 Pjh792 Haber Lab PMA1 under 2 copies of hsp promoter Pjh792 AmpR LEU2 2xHsp-PMA1
FB2720 Pjh746 Haber Lab Pjh746 AmpR URA3 PMA1
FB2728 pCK3 Use pCK4 for biotap-xl preps pCK3 pFA6a + Biotin - 2x TEV - 2x protein A
FB2729 pCK4 Use pCK4 for biotap-xl preps pCK4 pFA6a + 6xGLY - Biotin - 2x TEV - 2x protein A
FB2746 A02V Michael Springer Lab Derived from pFA6-kanMX. pFA6-HO-TDH3-YFP-ADH1-pTEF-kanMX-TEF-HO ampR pFA6-HO-TDH3-YFP-ADH1-pTEF-kanMX-TEF-HO
FB2747 A03V Michael Springer Lab Derived from pFA6-kanMX ampR pFA6-HO-TDH3-mCherry-ADH1-pTEF-kanMX-TEF-HO
FB2748 A05V Michael Springer Lab Derived from pFA6-natMX ampR pFA6-HO-TDH3-YFP-ADH1-pTEF-natMX-TEF-HO
FB2749 A06V Michael Springer Lab Derived from pFA6-natMX ampR pFA6-HO-TDH3-mCherry-ADH1-pTEF-natMX-TEF-HO
FB2755 Murray Lab pol3 mutator strain
Mol Cell Biol (2005)
vol. 25 (1) pp. 461-71 		PY24D pol3-L523D-URA3
FB2766 ASP1636 From Annika Guse, Stanford Human histone H1.4 ATCC #10701266. Digest with ECORI to remove form TOPO pASP1636 Kan
FB2768 pNHK53 Leon Chan Berkeley (Koshland) pADH1-osTIR1, URA3 integrating pNHK53 URA3 AmpR
FB2769 pTIR3 Leon Chan Berkeley (Koshland) pADH1-osTIR1, Leu2 replacing. PmeI digest pTIR3 pTIR3-LEU2 AmpR
FB2771 L260 Leon Chan Berkeley (Koshland) IAA7-3xV5, KanMx L260 AmpR KanR
FB2772 BYP6739 NBRP pCUP1-IAA17, kamMx BYP6739 AmpR KanR
FB2773 BYP6740 NBRP IAA17, KanMx BYP6740 AmpR KanR
FB2774 BYP7430 NBRP 3xminAID-KanMx BYP7430 AmpR
FB2775 BYP7431 NBRP 3xminAID -hphNP BYP7431 AmpR
FB2778 C81B Springer Lab, HMS HO-pTDH3-mcherry-Hgh-HO C81B AmpR mCherry
FB2779 D14B Springer Lab, HMS HO-pTDH3-BFP2-Hgh-HO D14B AmpR BFP2
FB2791 Aguilera Lab contains gal inducible RNasH1; from Herrera-Moyano...Aguilera, 2014 pRS416-gal-rnh1 pRS416-gal-rnh1 trp, amp
FB2790 Aguilera Lab contains gal inducible AID; from Garcia-Pichardo...Aguilera et al, 2017, Mol Cel pLZAID pSCH204-gal-aid ura, amp
FB2806 pMAX175 Boeke Lab Superloser kanR URA3 URA3syn CEN ARS pMAX175
FB2808 bJLW4 Hao Wu Lab His-MBP protein expression vector, N-terminal tag can be removed by 3C protease cleavage
 pDB-HisMBP-3C KanR
FB2809 bJLW5 Hao Wu Lab His-Smt3 protein expression vector, N-terminal tag can be removed by Ulp1 cleavage
 pSMT3 KanR
FB2817 RGBO5 Beate Schwer TRP1 CEN ampR CEG1
FB2818 RGBO6 Beate Schwer TRP1 CEN ampR ceg1-3
FB2819 RGBO7 Beate Schwer TRP1 CEN ampR ceg1-13
FB2820 RGBO8 Beate Schwer TRP1 CEN ampR ABD1
FB2821 RGBO9 Beate Schwer TRP1 CEN ampR abd1-5
FB2822 RGBO10 Beate Schwer TRP1 CEN ampR abd1-8
FB2823 CE113 Stephen Buratowski HIS3 CEN ampR CET1
FB2824 CE333 Stephen Buratowski HIS3 CEN ampR cet1-401
FB2825 CE339 Stephen Buratowski HIS3 CEN ampR cet1-438
FB2804 ESD422 Schwob lab GPD-TK (for BrdU incorp) in URA3 integrating plasmid
 pJJ242 URA3, ampR]
FB2844 pRS416 Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (1/4) pRS416
FB2845 pRS416 Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (2/4) pRS416
FB2846 pRS416 Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (3/4) pRS416
RB2847 pRS416 Remade pRS416, after the old glycerol stock in the -70 freezer started to fail to successfully yield colonies (4/4) pRS416
xit
rothstein
pGH127
pGH%
FB
FB2458 pBKS-1N Kingston Lab 5S rRNA nucleosomal positioning sequence
for mono-nucleosome assembly 		pBKS-1N 5S rRNA
FB2459 pBKS-2N Kingston Lab 5S rRNA nucleosomal positioning sequence
for di-nucleosome assembly 		pBKS-2N 5S rRNA
FB2460 pBKS-3N Kingston Lab 5S rRNA nucleosomal positioning sequence
for tri-nucleosome assembly 		pBKS-3N 5S rRNA
FB2461 pBKS-4N Kingston Lab 5S rRNA nucleosomal positioning sequence
for tetra-nucleosome assembly 		pBKS-4N 5S rRNA
pFW217
pRM200
pRM%
ura3 his3
snf1
ura3
%FLAG%
FB1611 BB778 J. Boeke "CAUTION"" DID NOT CONFER CANAVININE RESITANCE TO can1 MUTANT CELLS" " 9/99 unknown... pRS319
FB239 "J. Gorman, SmithKline for protein fusions to galK .. coli... pYSK9-8 amp TRP1 galK CEN3
FB240 "J. Gorman, SmithKline for protein fusions to galK .. coli... pYSK9-10 amp TRP1 'galK CEN3
FB241 "J. Gorman, SmithKline for protein fusions to galK .. coli... pYSK9-12 amp TRP1 'galK CEN3
FB1 DB6507 Botstein Can screen for URA3 function .......... pyrF74::Tn5 and all HB101 markers none
FB226 pPM702 "MCB 4, 657-665; Maynard Olsen contains an ochre suppressor .......... pPM702 SUP4-o CEN3 amp TRP1 ARS1
FB227 "MCB 4, 657-665, Maynard Olsen UGA suppressor .......... pPM600 SUP4-UGA CEN3 ampR TRP1 ARS1
FB1776 pAB204 pAb157 w/o BspI-RI fragment both BspI site and RI site still there .......... pAB204 LEU2 Amp (H3-H4d)d16'
FB1835 pRS401 Boeke lab .......... pRS401 "ampR, Met15
FB1836 pRS411 BOEKE LAB .......... pRS411 "ampR, MET15, CEN/ARS
FB1837 pRS421 BOEKE LAB .......... pRS421 "ampR, Met15, 2micron
FB1838 pRS402 Boeke lab .......... pRS402 "ampR, Ade2,
FB1839 pRS412 Boeke lab .......... pRS412 "ampR, CEN/ARS, ADE2
FB1840 pRS422 Boeke lab .......... pRS422 "ampR, Ade2, 2 micron
FB1841 pRS400 Boeke lab no s.cerevisiae marker.

KanMX is in opposite orientation from sequence provided by https://www.ncbi.nlm.nih.gov/nuccore/U93713.1. Confirmed by PCR using internal primers and corresponding flanking primers after integration into the genome 		.......... pRS400 "ampR, Kanmx4neomycin (G418)
FB1842 pGC25 Boeke lab no s. cerevisiae selectable marker .......... pGC25 "ampR, CEN6/ARS4
FB1843 pGC26 Boeke lab integrating marker .......... pGC26 "ampR, 2 micron
FB2023 pRM200 Grunstein lab "Mann & Grunstein, EMBO 11:9,3297 " .......... pRM200 "CEN, TRP1, HHT2, HHF2
FB2028 pRM430 Grunstein Lab "Mann & Grunstein, EMBO 11:9,3297 " .......... pRM430 "CEN, TRP1, HHT2 D4-30
FB2027 pRM435 Grunstein Lab "Mann & Grunstein, EMBO 11:9,3297 " .......... pRM435 "CEN, TRP1, HHT2 D4-35
FB2025 pRM440 Grunstein Lab "Mann & Grunstein, EMBO 11:9,3297 " .......... pRM440 "CEN, TRP1, hht2 D4-40
FB2024 pRM250 Grunstein Lab .......... pRM250 hht2 G9 G14 G18 G23
FB2026 pRM253 Grunstein Lab "Mann & Grunstein, EMBO 11:9,3297 " .......... pRM253 hht2 R9 R14 R18 R23
FB401 Himmelfarb and Friesen dominant suppressor of rpb2-ts mutation .......... pJH143 amp ura3 SRP1
FB402 Himmelfarb and Friesen dominant suppressor of rpb2-ts .......... pJH157 amp ura3 SRP1
FB403 Himmelfarb and Friesen wild type srp1 .......... pJH153 amp ura srp1+
FB576 Fred "H3-SphI insert, around 6 kb " .......... pFW199 pBR322 RSP5
FB577 Fred "H3-SphI insert, around 6 kb " .......... pFW200 YIp5 RSP5
FB578 Fred "H3-SphI insert, around 6 kb " .......... pFW201 YCp50 RSP5
FB579 "H3-SphI insert, around 6 kb " .......... pFW202 pCGS42 RSP5
FB636 M. Grunstein "see Cell 45, 445; Cell 35, 711 " .......... pJC102 HTA1 CEN3 URA3 ARS1
FB637 Grunstein "Cell 45, 445 " .......... pTS2 HTA1(d4-20) CEN ARS URA
FB638 Grunstein "Cell 45, 445 " .......... pTS9 HTA1d(124-131) CEN ARS URA3
FB639 Grunstein "Cell 45, 445 " .......... pTS10 HTA1d(4-29) CEN ARS URA3
FB640 Grunstein "Cell 45, 445 " .......... pMH12 HTA1(d114-131) CEN ARS URA3
FB641 Grunstein "Cell 45, 445 " .......... pMH13 HTA1(d30-45) CEN ARS URA3
FB717 Carol Lusty "Gene 45, 299-310; also see FB717-734; FB798-815 " .......... YEp353 'lacZ
FB718 C. Lusty see FB717 .......... YIp353 'lacZ
FB719 C. Lusty see FB717 .......... YEp354 'lacZ
FB720 C. Lusty see FB717 .......... YIp354 'lacZ
FB721 C. Lusty see FB717 .......... YEp355 'lacZ
FB722 C. Lusty see FB717 .......... YIp355 'lacZ URA3
FB723 C. Lusty see FB717 and notebook .......... YEp356 'lacZ URA3
FB724 C. Lusty see FB717 and notebook .......... YIp356 'lacZ URA3
FB725 C. Lusty see FB717 and notebook .......... YEp357 'lacZ URA3
FB726 C. Lusty see FB717 and notebook .......... YIp357 'lacZ URA3
FB727 C. Lusty see FB717 and notebook .......... YEp358 'lacZ URA3
FB728 C. Lusty see FB717 and notebook .......... YIp358 'lacZ URA3
FB729 C. Lusty see FB717 and notebook .......... YEp356R 'lacZ URA3
FB730 C. Lusty see FB717 and notebook .......... YIp356R 'lacZ URA3
FB731 C. Lusty see FB717 and notebook .......... YEp357R 'lacZ URA3
FB732 C. Lusty see FB717 and notebook .......... YIp357R 'lacZ URA3
FB733 C. Lusty see FB717 and notebook .......... YEp358R 'lacZ URA3
FB734 C. Lusty see FB717 and notebook .......... YIp358R 'lacZ URA3
FB766 L. Prakash overexpresses RAD6 in yeast .......... pSCW242 ADC-RAD6 2 micron TRP1 amp
FB767 L. Prakash "Genes and Dev. 2, 1476 " .......... pR619 ADC-rad6-149 2 micron TRP1 amp
FB768 L. Prakash "MCB 8, 1179 " .......... pR67 YCp50 RAD6
FB769 L. Prakash "MCB8, 1179 " .......... pR615 YCp50 rad6-149
FB770 L. Prakash "MCB 8, 1179 " .......... pR616 YCp50 rad6-153
FB771 L. Prakash "MCB 8, 1179 " .......... pR617 YCp50 rad6-164
FB786 Aaron Mitchell see FB674 for other IME1; do not give out; see notebook .......... pHS103 YEp24 IME1
FB787 Clyde Denis see notebook .......... pHH20 ADHI disruption
FB788 Clyde Denis .......... pHH21 ADHI disruption; see notebook
FB2042 GHB119 Snyder Lab vis Fink Lab for rescue of Snyder library URA+ insertions .......... pRSQLEU2 "ampR, LEU2
FB2043 pY2431 Young Lab .......... pY2431 "LEU2, CEN,RPB3
FB2044 pY2435 Young Lab .......... pY2435 "LEU2, CEN, HA1-RPB3
FB965 AB289 Kim Arndt . See enclosed sheet DO NOT GIVE OUT! See Science Aug87 vol.237 p.874; Nov89 vol.246 p.931 .......... AB289 URA3 ampR ori
FB966 AB328 Kim Arndt . See enclosed sheet DO NOT GIVE OUT! See Science Aug87 vol.237 p.874; Nov.8 9 vol.246 p.931 .......... AB328 URA3 ampR ori
FB967 B238 Hinnebusch. See enclosed sheet for references! DO NOT GIVE OUT! .......... B238 URA3 CEN ARS ampR ori GCN4
FB968 B401 Hinnebusch. See enclosed sheets. DO NOT GIVE OUT! .......... B401 URA3 CEN ARS gcn4::LEU2 ampR ori
FB1938 pAC153 Marian Carlson's lab ref. Abrams et al. MCB (1986) 6(11) 3643-3651 ..1....... pAC153 "2micron, URA3, SNF5, amp
FB1939 pEY111 Marian Carlson lab "ref. Estruch & Carlson, MCB 1986 10(6) 2544-2553 " ..1....... pEY111 "2 micron URA3, SNF6
FB319 D. Botstein "see Ng and Abelson, PNAS 77,3912 (1980) " ..101.n... pRB149 amp tet URA3
FB94 B255 Fink ..6507.... YEp24 amp tet (5 u/ml) URA3 2 micron
FB112 HB101 via Fink B324 in GR Fink collection .A300..... thr leu pro B1 supE44 r- m- recA
FB910 Tricia Davis .B1 ..n...
FB864 Anne Chiang no SPT9 function .B1.1..... pAC16 YCp50 SPT9 HindIII deletion clone
FB865 Anne Chiang no SPT9 function .B1.1..... pAC18 YCp50 SPT9 BglII clone
FB867 Anne Chiang not tested for function .B1.1..... pAC22 "SPT9, SacI piece reclosed
FB1440 .B1.1..... pT2
FB1441 .B1.1..... pT1-3B
FB1442 .B1.1..... pIP5469
FB180 Christy .B101..... spt3-242
FB186 deleted .B101.....
FB189 Christy .B101..... spt3-250
FB193 Christy .B101..... spt3-254
FB204 Christy .B101..... pFW32-265 spt3-265
FB206 Christy .B101..... pFW32-267 spt3-267
FB210 Christy .B101..... pFW32-271 spt3-271
FB214 Christy .B101..... pFW32-275 spt3-275
FB1992 pJK67 Pam Silver's lab .B101..... pJK67 "nuf2::GFP, URA3
FB1993 pJK91 Pam Silver's lab .B101..... pJK91 "nuf2::GFP, TRP1
FB367 B987 "PNAS 82, 8557 "small deletion, Nagawa and Fink " .B101..... x-99 HIS4-lacZ cen ura ars1 Xho linker
FB368 B994 "PNAS 82, 8557 Nagawa and Fink .B101..... x-407 HIS4-lacZ amp cen4 ars1 ura3 Xho linker
FB369 B1005 "PNAS 82, 8557 Nagawa and Fink .B101..... x-101 HIS4-lacZ cen4 ars1 ura3 Xho linker
FB370 B1006 "PNAS 82, 8557 Nagawa and Fink .B101..... x-122 HIS4-lacZ cen4 ars1 ura3 Xho linker
FB371 B1012 "PNAS 82, 8557 Nagawa and Fink .B101..... x-7 HIS4-lacZ cen4 ura3 ars1 Xho linker
FB372 B1015 "PNAS 82, 8557 Nagawa and Fink .B101..... x-198 HIS4-lacZ cen4 ura3 ars1 Xho linker
FB373 B1019 "PNAS 82, 8557 Nagawa and Fink .B101..... x-52 HIS4-lacZ cen4 ars1 ura3 Xho linker
FB439 DO NOT USE .B101.....
FB440 DO NOT USE .B101.....
FB485 .B101.....
FB528 Dave Hill do not give out .B101..... pS220 GPDH vector
FB529 Culbertson .B101..... pRG15-1 YIp5 SUF16-3
FB530 Culbertson .B101..... pRG17-1 YIp5 SUF16
FB531 Culbertson .B101..... pRG28 YIp5 SUF16-4
FB541 B89 G.R. Fink see map in notebook .B101..... pHIS4(Pst) pBR322 HIS4 Pst fragment
FB592 Roger Brent "see notebook and West et al MCB 4, 2467 " .B101..... LRdel20B GAL1-lacZ with Xho site between UAS and TATA
FB620 Stratagene "Bluescript KS polylinker, see notebook " .B101..... Bluescript m13+ KS polylinker Bluescript
FB621 Stratagene Bluescript .B101..... Bluescript m13+ "SK polylinker, Bluescript
FB622 Stratagene "bluescript, see notebook " .B101..... Bluescript m13- "KS polylinker, bluescript
FB623 Stratagene "bluescript, see notebook " .B101..... Bluescript m13- "SK polylinker, bluescript
FB631 Biorad via Dana Boyd see Biorad info (not in notebook) .B101..... dut- ung- thi- relA1 pCJ236 camR F' pox
FB667 Hieter for leu2 transplacement; see notebook .B101..... p307 YRp15 leu2del1
FB758 Botstein see notebook .B101..... pRB758 LEU2 polylinker
FB759 Botstein .B101..... pRB759 URA3 polylinker
FB761 M.A. Osley Beware - may be wrong - check it out first .B101..... pBL102 CYC1-lacZ
FB762 M.A. Osley Normalization probe for S1; see notebook .B101..... pHS2 RP51A
FB774 .B101..... pGC21
FB781 Kate Integrating version; in HB101 .B101..... pFW247 HTB1-lacZ amp
FB782 .B101.....
FB790 V. Zakian for overexpression; see notebook .B101..... YEpFAT10 leu2d
FB791 V. Zakian for overexpression; see notebook .B101..... YEpFAT4 leu2d
FB792 Stan Fields for Stan Fields interaction experiment; see notebook .B101..... pNY7 pUC18 GAL4 activation domain II
FB793 Stan Fields For Stan Fields experiment; see notebook .B101..... pMA424 HIS3 ADH1-GAL4(1-147) amp polylinker
FB836 Mark Johnston see notebook .B101..... pBM262 GAL1-HIS3 fusion URA3
FB258 RI 1.4 kb fragment contains TRP1 and ARS1 into RI site of pBR322 .B101.M15. YRp7 TRP1 ars1 tet amp
FB259 Rose and Botstein can be used for fusions .B101.M15. pRB45 LEU2 URA3 amp 2 micron 'lacZ
FB105 B620 Collaborative GAL UAS on 0.75 kb fragment; does NOT contain the GAL +1 of transcription .B101?.... CGE258 amp URA3 2 micron GAL UAS
FB142 DB6507 .B6507.... pFW32-349 spt3-349
FB376 F. del Rey and G.R. Fink RsaI fragment of sigma plasmid into ClaI of YIp5 .C1061.... pML52 URA3 sigma
FB557 Jan Fassler .C1061.... pJF182
FB327 B80 R. Davis via G. Fink "R1 fragment with Ty and delta. Cell 16, 739 (1979) " .C1065.... S13 amp Ty
FB1986 pDR14 Dave Rose .H5a......
FB2111 pJM196 J Madison human yeast SPT3 chimera .H5a...... pJM196 "LEU2,2u,Sc1-258,human 242-317
FB2113 pJM202 J. Madison HUMAN YEAST CHIMERA .H5a...... pJM202 "p425MET25,Sc99-337,human1-109
FB521 Gaber and Culbertson 0.55 kb insert .K8018.... RG2-2 YIp5 suf16+ HindIII-SalI
FB1989 .L-1 Blue. HDF1D::TRP1 TRP1 HDF1D
FB1777 pAB221 Alex. pAB157 w/o aa 4-30 in H3 .nknown... pAB221 LEU2 Amp (H3d4.30-H4)
FB1778 pAB224 Alex. pAB157 w/PCR fragment from FY869 carrying bur5-1 mutation .nknown... pAB224 LEU2 Amp.
FB648 Christine out of frame fusion .R1....... E2 pATH1 SPT8 Bam-Bam
FB649 Christine out of frame fusion .R1....... B3 pATH2 SPT8 Bam-Bam
FB834 Steve Hahn from Steve Hahn; do not give out; do not use .R1.1..... pSH229 GAL-SPT15
FB739 Celenza and Carlson for making snf1 null; see notebook .R9.0..... pCN106 snf1 deletion
FB740 Celenza and Carlson SNF1 probe; see notebook .R9.0..... pCN8 SNF1
FB741 Celenza and Carlson see FB739 in notebook for information; for making snf4 null .R9.0..... pFE27-32b snf4 deletion
FB742 Celenza and Carlson "SNF4 probe, see FB739 in notebook for more information " .R9.0..... pC8 SNF4
FB2461 4N Bob Kingston for assembling a tetra-nucleosome array ? 4N
FB2460 3N Bob Kingston for assembling a tri-nucleosome array ? 3N
FB2459 2N Bob Kingston for assembling a di-nucleosome array ? 2N
FB2458 1N Bob Kingston For assembling a mono-nucleosome ? 1N
FB2079 pBM1530 M Johnston "integrating plasmid, YEAST 13:127-137(1997) " ?......... pBM1530 "URA3, UAS-LEU2(-125 to-405), HIS3(from -79)
FB2080 pBM3068 M Johnston Lab Yeast13:127-137(1997) ?......... pBM3068 "URA3,2micron,+306 to-79HIS3-LacZ,-784to-135SUC2prom
FB2108 pPWDSGS1 Ed Louis disruption plasmid ?......... pPWDSGS1 "LEU2, SGS1
FB2244 pGN1622 Jeff Boeke ?......... pGN1622 "Spt10-C387S, LEU2, pRS304
FB2245 pGN961 Jeff Boeke ?......... pGN961 "SPT21, CEN, LEU2, YCp50
FB2253 Gal1-FLP1 Snyder lab to cure endogenous 2micron from yeast strain ?......... YEp351-Gal-FLP1 "2micron, LEU2, Gal1p-FLP1
FB2296 pCK22 Craig Kaplan Spt5cDNA clone from BDGP via Genome Systems ?......... pCK22 "pBSIIK+ LD10265, Amp
FB2297 pCK151 Craig Kaplan Spt4cDNA BDGP via Research Genetics ?......... chloramphenicol pCK151 pOT2a LD44495 Spt4cDNA
FB2401 pLS21-5 Karen Arndt pRS314 based ?......... pLS21-5 HA-RTF1 CEN TRP1
FB1848 pIL35 E. Dubois see reference in book ?......... pIL35 "ampR, URA3, CEN4/ARS1, Ty Region A, TY2 PvuII-Sau3, TDH3 DUAS-la cZ
FB1849 pIL37 E. Dubois see reference in book ?......... pIL37 "ampR, URA3, CEN4/ARS1, Ty Region A- TDH3 DUAS-lacZ, Ty2=PAE1-6, PvuII-Sau3
FB1850 pIL40 E. Dubois see reference in book ?......... pIL40 "ampR, URA3, CEN4/ARS1, Ty REgion-A, PvuII-Sau3 Ty1 +PAE2-6, TDH3 DUAS-lacZ
FB1851 pcI-13 E. Dubois see reference in book ?......... pcI-13 "ampR, tec1::URA3
FB1852 p6/50 Stan Field via Elaine Elion ?......... P6/50 "ampR, URA3, CEN, STE12
FB96 B292 Collaborative same as pCGS42 except 2 micron fragment in opposite orientation ?......... pCGS44 URA3 2 micron amp tet
FB106 B621 Collaborative "GAL UAS on 0.75 kb fragment, Xho cloning site " ?......... GAL UAS URA3 amp
FB107 B622 Collaborative GAL UAS with Sal cloning site ?......... "GAL UAS, URA amp 2 micron
FB1775 pAB157 w/o HIII msc. fragment ?......... pAB203 LEU2 Amp (H3d.H4)d16'
FB1811 Audy Hoyt bub2::URA3 ?......... pTR24 ClaI SacI
FB1847 pIL30 E.Dubois see refrence in book ?......... pIL30 "ampR, URA3, CEN4/ARS1, Ty RegionA-, TDH3 DUAS-lacZ, PvuII-SAU3
FB1937 pLN138-4 Marian Carlson lab "ref. Abrams et al, MCB (1986) 6(11) 3643-3651 " ?.1....... pLN138-4 "2micron, URA3, SNF2, amp
FB2032 pAD54 Dr. Wigler's lab ?.1....... pAD54 "LEU2, 2micron, ADH1-p, ADH1-T
FB160 B90 Fink 5' Sal fragment of HIS4 in YIp5 ?.1.3..... YIp301 URA3 HIS4A
FB161 B131 Fink RI eviction of his4-917; see map in strain book ?.1.3..... phis4-917 URA3 his4-917
FB118 B155 "Fink, Phil Carter orientation of insert unknown ?.101..... B155 Ty912 HIS4 URA3 amp
FB176 Fields and Herskowitz This contains an alpha factor-lacZ fusion in YCp50 ?.101..... YCp50::fz46 alpha factor lacZ URA
FB2231 RY2973 Nancy Woychik ?.101..... RY2973 "URA3, SRB10, CEN
FB2286 pGY107 Jan Fassler ?.101..... pGY107 "CEN,URA3,MYC-MCM1
FB318 B163 R. Surosky and G.R. Fink see notebook for exact map of fragment ?.101.n... B163 ampR Ty S13 BglII fragment
FB115 ? D. Hanahan high frequency transformer ?.1046.... ? none
FB174 c12 Jensen and Herskowitz may not be regulated properly ?.2186.... YCp50-HO::lacZ HO::lacZ URA3 amp
FB1800 GHB99 S. Buratowski ?.5-alpha YEpPPR2 URA3 2micron PPR2
FB1805 M. Ptashne see book ?.5-alpha. S023 gal11 CEN LEU2
FB1995 BHM270 Fink Lab ?.5a...... BHM270 "ampR, ori, STE11-4, LEU2, CEN
FB1996 BHM271 Fink Lab ?.5a...... BHM271 "ampR, ori, CEN, HIS3, STE11-4
FB1997 BHM406 Fink Lab ?.5a...... BHM406 "ori, ampR, STE11-4, CEN, TRP1
FB1999 pJEF2017 Jeff Boeke ?.5a...... pJEF2017 "2micron, TRP1, URA3, CUP1-TY
FB2000 pJEF1908 Boeke lab ?.5a...... pJEF1908 "URA3, 2micron, CUP1-TY
FB2034 W. Bandlow lab ?.5a...... puc19-leo::URA3 "leo1D::URA3, puc19 bckbone
FB2035 W. Bandlow lab ?.5a...... YEP352-LEO1 LEO1 in YEP352
FB2036 pMB284 Munrice Basrai ?.5a...... pMB284 "2micron, LEU2, AmpR, ADH1p-HA1-SPT4
FB2106 pCK17 Craig Kaplan ?.5a...... DmSPT5-ESTD08338 pBluescript KS - Backbone
FB2214 pRSQ2-URA3 Snyder lab for use with the Tn3-LEU2-LacZ library ?.5a...... pRSQ2-URA3
FB2216 PHJM1 B OLSEN ?.5a...... PHJM1 "pBluescript, 5' end of SUPT3H cDNA
FB2217 p123089 B Olsen pBluescript derivative ?.5a...... p123089 Est clone p123089 into pT7T30
FB2242 pRP82 N Woyzhick ?.5A...... pRP82 "RPB8, pBSK2(-)
FB90 B6 via Fink ?.6507.... pBR322
FB91 B46 via Fink ?.6507.... YEp13 LEU2 amp 2 micron
FB92 B161 Fink "Ty probe, internal " ?.6507.... B161 "amp, Ty 1.2 BglII fragment
FB93 B205 Fink Ty2 probe ?.6507.... Ty917 Cla amp cam Ty917 Cla fragment
FB95 B291 Collaborative 2 micron DNA contains an XBA site ?.6507.... pCGS42 URA3 tet amp 2 micron
FB2183 pJT10 David Amberg based on YEplac195 ?.B4...... pJT10 "ADE8, URA3
FB911 Tricia Davis AB1886 F- uvrA6 thr1 leu6 thi1 lacV1 galkz ara14 xyl5
FB912 Tricia Davis SEE FB911 and enclosed information in notebook AB1886.... pGW249 pKM10bla::Tri5 kanR
FB352 AR68 SmithKline good for production of hybrid proteins; see notebook AR68.5....
FB11 none Winston insert is 5.3 kb B28.32.... trp B1 his (P2 eductant) pFW1 "E. coli hisOGD H3 in YIp5, amp
FB12 none Winston opposite orientation of pFW1 B28.32.... trp B1 his (P2 eductant) pFW2 "E. coli hisOGD in YIp5, amp, weak tet
FB22 B28lam322 Ralph Isberg B28.60.... see FB4 lambda 322 b5a5 cI857 nin Sam7 Tn903(b2)
FB406 BD1528 Bruce Duncan BD1528.... ung1 nadB30 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 galT22 r psL relA1(?) none
FB405 BD817 Bruce Duncan "Gene 28, 211-219 (1984) " BD817..... ung1 nadB7 recA56 thi1 metB1 trpR55 hsdR514 supE44 supF58 lac galK2 ga lT22 rpsL relA1(?) none
FB1394 pJH569 Jim Haber see sheets. BJH2..n... pJH569 URA3 ampR TSM1 MATa inc.
FB1393 pJH662 Jim Haber see sheets. BJH5..n... pJH662 ampR MATalpha tsm1::LEU2
FB2152 pJM182 J.Madison BL21 pJM182 6HisNterm-MOT3 pet vector
FB2495 pET28b-H2A Carl Wu Supercodon plus strain expressing H2A1 BL21 pET28B-H2A1 H2A1
FB2496 Carl Wu H2B1 in supercodon plus strain BL21 pET28b-H2B1 H2B.1
FB2497 Carl Wu H3.2 supercodon plus strain BL21 pET28b-H3.2 H3.2
FB2498 Carl Wu H4.2 supercodon plus strain BL21 pET28b-H4.2 H4.2
FB885 F.W. Studier FB885-894 are for expression via T7 RNA polymerse. See Fred or Greg for the article describing all of these vectors and strains. BL21 (DE3) pLysS
FB888 F.W. Studier see FB885 BL21 (DE3) none
FB1025 pKA9 Karen Arndt "using oligo-directed mut., an NdeI site was placed at the SPT15 ATG. The 11 43bp NdeI-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid.* *DO NOT GIVE OUT** " BL21(DE3). pLysS (is a plasmid carrying the T7 lysozyme gene =it is CmR). BL21(DE 3) is a lamda lysogen contai pKA9 ampR SPT15+
FB1027 pKA10/pet Karen Arndt "using oligo-directed mut, an NdeI site was placed at the spt15 ATG. The 114 3bp NdeI-BamHI frag. was inserted into pET3b, a T7 overexpressor plasmid. **SEE FB1025!** " BL21(DE3). pLysS (a plasmid carrying the T7 lysozyme gene; it is CmR) BL21(DE3) is a lmabda lysogen containi pKA10 AmpR spt15-122 CmR
FB1029 pKA11 Karen Arndt "using oligo-directed mut. an NdeI site was placed at the spt15 ATG. The 114 3bp NdeI-BamHI frag was inserted into pET3b, a T7 overexpressor plasmid. **SEE FB1025!** " BL21(DE3). pLysS (a plasmid carrying the T7 lysozyme gene; it is CmR) BL21(DE3) is a lambda lysogen containi pKA11 AmpR spt15-21 CmR
FB1377 pBBB2 Barbara Berg =NdeI-BamHI frag containing SPT3 from pDE118-8 into NdeI-BamHI of pET-3C (FB 894) SEE SHEETS IN NOTEBOOK. BL21(DE3). see enclosed sheets for info on host. Bl21(DE3) also contains pLysS w hich is CmR. pBBB2 T7 promoter ampR SPT3 CmR
FB2287 p-GCN5 for making recombinant GCN5 for HAT assays BL21(DE3). pRSetB-GCN5 GCN5
FB887 F.W. Studier see FB885 BL21.(DE3) none
FB1846 pSR73 SR "Bacterial GST-Spt20 expression vector, C-terminus " BL21...... pSR73 "GST-Spt20, AA437-604
FB2351 CyclinT David Price via Craig for affinity purifying Cyclin T antibodies BL21...... pCK150 "T7-GST-DmCyclinT, pET vector
FB2492 BL21/DE3 Rolf Sternglanz Super codon plus strain for protein expression BL21/DE3 Chloramphenicol
FB2134 pJM111 J.Madison BL21/DE3.. pJM111 T7prom. His6-HA-SPT3
FB1497 5309 Lindquist Lab see enclosed sheet. BSJ-72.... pYABU11 ampR hsp104::URA3 lacZ
FB1498 5310 Lindquist Lab see enclosed sheet BSJ-72.... pYABL5 ampR hsp104::LEU2 lacZ
FB1688 C600 T. Lane (Leder lab) original C600 isolate was EcoK r+m+ McrBC+ **See also BNN93 with enclosed s heet in notebook** C600...... F- e14- (McrA-) thr-1 leuB6 thi-1 lacY1 supE44 rfbD1 fhuA21 EcoK r+m+ McrBC+
FB475 CAG10153 Carol Gross This is a nonsense rpoH allele. Grow the strain at 30 or 35. Ts best seen at 42. See notebook for details. CAG10153.. MC1061 rpoH165 zhf50::Tn10 none
FB476 CAG1578 Carol Gross "Ts rpoD allele with linked Tn10. 25-30 is permissive, 42 is nonpermissive. Seenotebook for details. " CAG1578... MC1061 rpoD800 Tn10 none
FB1615 Golberg . Good host for expressing foreign proteins in bact. Clp- Clp-
FB2826 pML104 Laughery et al Yeast 2015 via K. Struhl Cas9 and gRNA expressing plasmid for CRISPR editing in yeast
 dam- pML104 URA3, AmpR, 2u
FB2827 pML107 Laughery et al Yeast 2015 via K. Struhl Cas9 and gRNA expressing plasmid for CRISPR editing in yeast
 dam- pML107 LEU2, AmpR, 2u
FB134 Winston DB6507 pFW32-330 spt3-330
FB2 B44/YIp5 Fink DB6507.... HB101 pyrF-74::Tn5 YIp5 amp tet URA3
FB3 B44/YIp5 G.R. Fink "plasmid very unstable in yeast, even when selected " DB6507.... HB101 pyrF74::Tn5 YRp10 "ARS1, URA3, amp
FB13 B44/pFW3 Winston YIp5 with the HindIII site destroyed by filling in with reverse transcriptas e DB6507.... HB101 pyrF74::Tn5 pFW3 URA3 amp
FB14 B44/pFW4 Winston SalI site destroyed by filling in with reverse transcriptase DB6507.... HB101 pyrF74::Tn5 pFW4 "URA3, amp
FB15 B44/pFW5 Winston orientation 1 DB6507.... HB101 pyrF74::Tn5 pFW5 "URA3, amp, HIS4 EcoRI fragment
FB16 B44/pFW6 Winston orientation 2 DB6507.... HB101 pyrF74::Tn5 pFW6 "URA3, amp, HIS4 EcoRI fragment
FB25 B44/pFW9 Winston The his fragment is RI-Pst. The plasmid is ampS tetR. DB6507.... pFW9 URA tet Salmonella hisOGD
FB26 Dickson Check Fred's notes; this is the b-gal gene from K. lactic DB6507.... pKl6 K. lactis b-gal
FB28 Winston DB6507.... pFW10 "tet, URA3, Salmonella hisOGD, ars1
FB29 Johnson and Abelson "SUP6 is an Alu2 fragment with Bam linkers, 750 bp long, tRNAtyr " DB6507.... pYSUP6-Alu2 "amp, SUP6
FB30 Winston HIS4 Sal fragment in YIp5; lacZ Xho fragment in Xho site in HIS4; lacZ fragm ent is from pMC593 DB6507.... pFW11 amp URA3 lacZ HIS4
FB31 Winston "same as pFW11, but opposite lacZ orientation " DB6507.... pFW12 "amp, HIS4, URA3, lacZ
FB34 Winston SUP6 Bam fragment in YEp13 DB6507.... pFW13 LEU2 SUP6 2micron
FB35 Winston "derived from YIp5 and pYeCEN3-41; see Nature 287, 504 " DB6507.... pFW14 ars1 CEN3 URA3 amp
FB36 Winston YIp5 with the HIS4 5' RI fragment; URA3 is inserted in the HIS4 HindIII site DB6507.... pFW7 URA3 HIS4
FB37 Winston same as pFW7 except opposite URA3 orientation; in this plasmid it is in the sameorientation as HIS4 DB6507.... pFW8 URA3 HIS4 amp
FB39 Winston used for 3' experiment DB6507.... pFW15 amp HIS4 241 bp H3-RI fragment
FB40 Winston used for 3' experiment DB6507.... pFW16 amp HIS4 279 bp H3-RI
FB41 Winston Original SPT3 clone candidate 1.6 DB6507.... pFW17 URA3 2 micron SPT3
FB42 Winston Original SPT3 clone 2.37(3-4) DB6507.... pFW18 URA3 SPT3 2 micron amp
FB43 Winston Original SPT3 clone 3.1 DB6507.... pFW19 URA3 SPT3 2 micron amp
FB44 Winston DB6507.... pFW21 YRp10 with SPT3 1.7 Sal fragment
FB45 Winston . DB6507.... pFW20 YRp10 with 1.2 kb RI fragment from SPT3
FB46 Winston This clone has SPT3 function DB6507.... pFW23 pCGS142 with SPT3 H3-BglII fragment
FB47 Winston DB6507.... pFW24 pBR322 with SPT3 H3-BglII fragment
FB48 Winston pFW27= pFW4 with 8kb HindIII frag containing SPT3 DB6507.... pFW27 SPT3 URA3 ampR
FB49 Winston DB6507.... pFW22 pFW19 with 2 small Sal fragments deleted
FB50 Winston DB6507.... pFW28 pFW27 with 1.7 kb Sal fragment deleted
FB52 Winston DB6507.... pFW29 SPT3 H3-BglII URA3 amp
FB53 Winston SPT3 fragment in pFW4 DB6507.... pFW30 SPT3 H3-BglII URA3 amp
FB54 Winston SPT3 H3-BglII in pCGS42 DB6507.... pFW31 SPT3 H3-Xho URA3 amp 2 micron
FB55 Winston DB6507.... pFW32 SPT3 RI-BglII URA3 2 micron amp
FB63 Winston derived from pFW30 DB6507.... pFW33 amp URA spt3-101
FB64 Winston spt3 deletion that goes outside the gene DB6507.... pFW34 amp URA spt3-201
FB65 Winston RI-Xho of SPT3 in RI-Sal of pBR322 DB6507.... pFW42 amp SPT3
FB67 Winston "WRONG, DO NOT USE " DB6507.... pFW44 "WRONG, DO NOT USE
FB66 Winston RI-Xho of SPT3 in YIp5 DB6507.... pFW43 amp URA SPT3
FB68 JT503 J. Thorner RI-H3 of MATa DB6507.... JT503 MATa
FB69 Winston pFW14 plus HIS4 5' Sal fragment in undetermined orientation DB6507.... pFW38 HIS4 5' amp
FB70 Winston same as pFW38 except has the 912delta Sal fragment DB6507.... pFW39 his4-912delta URA amp
FB74 Winston see FB70 DB6507.... pFW41 same as pFW39 but 2 Sal fragments inserted
FB78 Rose and Winston DB6507.... pMRFW2 ura3-52 amp URA3
FB79 Jensen and Herskowitz DB6507.... YCp50-HO HO URA3 CEN4 ARS1
FB80 J. Abraham via Bik Tye H3 fragment of MATalpho into the H3 site of YEp13 DB6507.... alpha*7.375 MATalpha LEU2 2 micron
FB83 Winston HIS4 Bgl-Sal (1.2 kb) into pBR322 Bam-Sal DB6507.... pFW45 amp HIS4
FB84 Collaborative 0.85 kb GAL fragment; for PROTEIN fusions DB6507.... pCGS286 GAL UAS 'lacZ URA3 2 micron
FB88 G.S. Roeder DB6507.... R338 amp Ty1-17 URA3
FB89 Winston deletion of Xho fragment in R338 DB6507.... pFW46 amp URA3 1-17delta
FB128 . DB6507.... pFW32-318 spt3-318
FB129 . DB6507.... pFW32-319 spt3-319
FB130 . DB6507.... pFW32-322 spt3-322
FB131 Winston DB6507.... pFW32-326 spt3-326
FB132 Winston DB6507.... pFW32-327 spt3-327
FB133 Winston DB6507.... pFW32-328 spt3-328
FB135 Winston DB6507.... pFW32-332 spt3-332
FB136 Winston DB6507.... pFW32-334 spt3-334
FB137 Winston DB6507.... pFW32-335 spt3-335
FB138 Winston DB6507.... pFW32-336 spt3-336
FB139 Winston DB6507.... pFW32-339 spt3-339
FB140 Winston DB6507.... pFW32-343 spt3-343
FB141 Winston DB6507.... pFW32-343 spt3-343
FB143 Winston DB6507.... pFW32-354 spt3-354
FB144 Winston DB6507.... pFW32-355 spt3-355
FB145 Winston DB6507.... pFW32-360 spt3-360
FB146 Winston DB6507.... pFW32-364 spt3-364
FB147 Winston DB6507.... pFW32-367 spt3-367
FB148 Winston DB6507.... pFW32-370 spt3-370
FB149 Winston DB6507.... pFW32-374 spt3-374
FB150 Winston DB6507.... pFW32-377 spt3-377
FB151 Winston DB6507.... pFW32-379 spt3-379
FB152 Winston DB6507.... pFW32-382 spt3-382
FB169 B231 Fink HIS4 region from RI site 5' to H3 site 3'. All of HIS4 is not included. DB6507.... phis4-917delta his4-917delta URA3 amp
FB231 GRF559 Giora Simchen Partial eviction of lys2-61 to the EcoRI site DB6507.... pGS4 amp URA3 lys2-61 (partial)
FB232 GRF560 Giora Simchen Complete Ty61 (lys2-61) on this plasmid DB6507.... pGS5 amp URA3 lys2-61
FB235 Christy subclone of LYS2 RI-H3 into YCp50 DB6507.... pCC2 amp URA3 LYS2 CEN
FB978 B835 Mark Rose this plasmid is YCp50 with the SalI site filled in creating a NEW PvuI site DB6507.... pMR78 URA3 ampR tetS
FB979 B836 Mark Rose this plasmid is YCp50 with the XhoI site filled in creating a NEW PvuI site. CEN function is not affected. DB6507.... pMR79 URA3 ampR tetR
FB6 RB18 Botstein DB6656.... trp(am) lac(am) pyrF::Mu su- YIp32 "LEU2, amp
FB7 RB19 Botstein "Gene 8, 17-24 (1979) " DB6656.... trp(am) lac(am) pyrF::Mu su- YIp33 "LEU2, amp
FB8 RB20 Botstein "Gene 8, 17-24 (1979) " DB6656.... trp(am) lac(am) pyrF::Mu su- YIp29 LEU2 URA3 amp
FB9 RB21 Botstein "Gene 8, 17-24 (1979) " DB6656.... trp(am) lac(am) su- pyrF::Mu YIp28 LEU2 URA3 amp
FB20 DB6660 Botstein DB6660.... trp(am) lac(am) pyrF::Mu hsr- hsm+ supF none
DB21 RB39 Botstein "ura3-18 on Bam fragment, LEU2 on Sal/Xho " DB6660.... see FB20 pRB39 amp ura3-18 LEU2
FB27 Dickson "see Fred's notes, contains K. lactis b-gal " DB6660.... pKl6 K. lactis b-gal
FB153 Christy DB6660.... trp(am) lac(am) pyrF::Mu r- m+ supF pCC1 SPT3 on RI-XhoI fragment in YCp50
FB534 Jules O'Rear Plasmid is probably WRONG. DH1....... pFW194 spt3-202::URA3-2 amp URA3
FB535 Jules O'Rear "BEWARE, plasmid may not be correct " DH1....... pFW195 spt3-202::URA3-10 amp URA3
FB1323 DH10/P3 Grant Hartzog E. coli strain for propagating supF plasmids DH10/P3... "F- mcrA d(mrr-hsd RMS-mcrBC) 0 80d lacZdM15 dlacX74 deoR recA1 arad139 a(ara, leu)7697 galu galk
FB1485 pGH95 Hartzog / Hovasse RI-XhoI frag from pGH77 (FB1463) containing hSPT4 inserted into RI/XhoI cut pTAG DH10/P3... tetR pGH95 hSPT4 SupF ampR tetR
FB1486 pGH96 Hovasse / Hartzog RI-XhoI frag from pGH78 (FB1464) containing SPT4 into RI/XhoI cut pTAG DH10/P3... tetR pGH96 SPT4 SupF ampR tetR
FB1266 GAL1-cDNA Tony Bretscher GAL1-cDNA library REF: Genetics 132 (1992) p. 665-673 DH10B GAL1-cDNA library
FB1825 B3259 Haoping Liu & Gerry Fink $.8 kb insert containing FLO8 isolated from HL's genomivc library in pRS316 DH10B..... pHL1 "amp, puc orig, f1 ori, URA3, FLO8
FB1636 GHB54 Hartzog via Soares from image consortium Genbank#= T80145 DH10B.ha.. 24522 ampR lafmidBA potential human SPT5 cDNA
FB1637 GHB55 Hartzog via Soares from image consortium (nearly identical to 24522=FB1636) Genbank#= R14898 DH10B.ha.. 30004 ampR lafmidBA potential human SPT5 cDNA
FB1638 GHB56 Hartzog via Soares from image consortium Genbank#= R18289 DH10B.ha.. 30813 ampR lafmidBA potential human SPT5 cDNA
FB2691 DH10Bac Invitrogen DH10Bac bacmid pMON4274-kan helper plasmid pMON7124-tet
FB1301 LR1d1 Roger Brent GAL1 deleted UAS leaves XhoI site. NO LexA ops. this strain is the negati ve control for FB1302-FB1306. DH5 (?). LR1d1 GAL1dUAS-lacZ URA3 ampR 2micron
FB1300 pdACR1 Roger Brent DH5 (?) pdACR1 acr1::URA3 ampR LEU2
FB1299 pACR1 Roger Brent DH5 (?).. pACR1 ACR1 ampR URA3
FB1302 pSH18-18 Roger Brent "see also FB1301, FB1303-1306. " DH5 (?).. pSH18-18 GAL1dUAS-lacZ URA3 2u ampR LexA ops. (6)
FB1303 pRB1840 Roger Brent "see also FB1301-1302, FB1304-1306 " DH5 (?).. pRB1840 GAL1dUAS-lacZ URA3 2u ampR LexA op.
FB1304 pSH18-34 Roger Brent "see also FB1301-1303, FB1305-1306 " DH5 (?).. pSH18-34 GAL1dUAS-lacZ URA3 2u ampR LexA ops (8)
FB1305 pSH18-8 Roger Brent "see also FB1301-1304, FB1306 " DH5 (?).. pSH18-8 GAL1dUAS-lacZ URA3 ampR 2u LexA ops. (4)
FB1306 SH1834dspe Roger Brent see also FB1301-1305 DH5 (?).. pSH18-34dspe GAL1dUAS-lacZ URA3 ampR integrating LexA ops.
FB1324 pSR1-2 Shannon Roberts 7.3kb Cla-RI frag from pFW127 inserted into Cla-RI of pRS404 DH5 alpha. pSR1-2 SPT7 ampR TRP1
FB1326 pSR3 Shannon Roberts #NAME? DH5 alpha. pSR3 URA3 2u ampR
FB1327 pSR4-5 Shannon Roberts constructed by cutting out 3kb Sal-Spe SPT8 frag from pMG1 and inserting it into Sal-Spe of pSR3 (FB1326) ***for epitope tagging SPT8*** DH5 alpha. pSR4-5 SPT8 URA3 2u ampR
FB1328 pSR5 Shannon Roberts 3kb SPT8 Sal-Spe frag into Sal-Spe of pRS426 DH5 alpha. pSR5 SPT8 URA3 2u ampR
FB1329 pSR6-3 Shannon Roberts SPT8 tagged at N term. DH5 alpha. pSR6-3 SPT8-myc epitope URA3 ampR 2u
FB1330 pSR7-3 Shannon Roberts 3.1kb Sal-Spe SPT8-N term myc epitope tagged prot. from pSR6-3 (FB1329) into Sal-Spe of pRS424 DH5 alpha. pSR7-3 SPT8-myc epitope TRP1 ampR 2u
FB1810 D. Stillman DH5-a.pha. m1977 "rgr1, TRP1 disruptor
FB1650 pPS793 P. Silver lab ADE3 in pRS424 Spe1/BamH1 sites see pPS794 for unique sites **used for secto ring assays.**** DH5-alpha pPS793 "ADE3, TRP1, 2micron, ampR
FB1799 GHB98 C Kane Lab linearized w/ PvuII-XhoI DH5-alpha pJD3 Amp ppr2d::HISG-URA3
FB1796 GHB95 c. Kane lab DH5-alpha. pkc16(1-309) TRP1 CRN Amp ARS PPR2
FB1797 GHB96 C. Kane Lab DH5-alpha. pkc13 GAL1-PPR2 2micron Amp
FB1798 GHB97 CKane Lab DH5-Alpha. pJD5 "Amp, LEU2, CRN, ARS, pp22
FB1803 D. Stillman DH5-alpha. m1979 "rgr1, LEU2 disruptor
FB1804 D. Stillman DH5-alpha. m2597 "RGR1, URA3, 2micron
FB1286 pAB24 Chiron Corp. (T. Brake) ***K. lactis library*** REF: NAR vol.19 No.19: 5345-5350. See Yeast Library notebook DH5.own... pAB24 K. lactis library URA3 ampR
FB1981 pEMBLSup35 Irina Derkatch DH5a pEMBL-SUP35 "URA3, LEU2-D 2micron, SUP35
FB2486 pYCF4 Hieter lab CHL1 in centric vector pYCF4 DH5a pYCF4 CHL1 ARS3 SUP11 CEN4 TEL cassette
FB2499 CKB25 Craig Kaplan SPT6 with amino terminal FLAG tag cloned into YCp50 DH5a pCK25 SPT6-FLAG URA3 CEN
FB2500 CKB40 Craig Kaplan amino terminal FLAG tagged SPT6 in pRS306 DH5a pCK40 SPT6-FLAG URA3
FB2501 CKB125 Craig Kaplan amino terminal FLAG tagged spt6-1004 in pRS306 DH5a pCK25 spt6-1004-FLAG URA3
FB2502 3xHA-TBP Kevin Struhl DH5a 3xHA-TBP 3xHA-TBP URA3 CEN
FB2503 pDM776 D. Moazed DH5a pFA6a-kanMX6-p3nmt1 kanMX6 nmt1 promoter
FB2504 pDM777 D. Moazed DH5a pFA6a-kanMX6-P41nmt1 KanMx6 attenuated nmt1 promoter
FB2505 pDM778 D. Moazed DH5a pFA6a-kanMX6-P81nmt1 kanMX6 attenuated nmt1 promoter
FB2506 pDM745 D. Moazed DH5a pFA6akanMX6P3nmt13HA kanMX6 3HA nmt1 promoter
FB2507 pDM746 D. Moazed DH5a pFA6akanMX6P41nmt13H kanMX6 P41nmt1 3HA
FB2508 pDM747 D. Moazed DH5a pFA6akanMX6P81nmt13H kanMX6 P81nmt1 3HA
FB2509 pKG1991 D. Moazed DH5a pREP1-NT LEU2 Pnmt1 Tnmt1
FB2510 pKG1992 D. Moazed same as FB2509 except extended polylinker/multiple cloning site DH5a pREP41-NT LEU2 Pnmt1 Tnmt1
FB2511 pKG1993 D. Moazed DH5a pREP81-NT pREP81-Pnmt1 Tnmt1 LEU2
FB2512 pKS-ura4 DH5a DH5a p358 pBluescript Sp. ura4
FB2513 p1810 K.Gould DH5a pFA6a-kanMX6-CTAP2 kanMX6 2xproteinA TAP tag
FB2514 p2021 K. Gould DH5a pFA6a-KanMX6-CTAP4 kanMX6 4xproteinA TAP tag
FB2515 p1810-NAT Amine DH5a p1810-NAT replace kanR with NatR
FB2516 p2845 N. Rhind p2021 / BamH1-Pac1 DH5a pFS209 3xHA 5' TAP tag
FB2517 p30412 Euroscarf Yeast 2005;22:1061-1068 DH5a pFA6a-3HA-natMX6 3xHA natMX6

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