Winston Lab Strain Finder
Department of Genetics

Bacteria


Displaying 1 - 500 of 2807
Contains
Strain Name Strain Alias Source Comments Host Host Markers Plasmids Plasmid Markers
FB2516
pMS37
psh17-4
FB2114
%FB2114%
fb2044
fb843
fb843
pLBS27
pLBS27
pMRFW2
fb603
FB2694 Yeast 2009; 26: 185-192 pFA6-6xGLY-FLAG-KanMX 6xGLY-FLAG-KanMX
FB2695 Yeast 2009; 26: 185-192 pFA6-6xGLY-3xFLAG-KanMX 6xGLY-3xFLAG-KanMX
FB2696 Yeast 2009; 26: 185-192 pFA6-6xGLY-Myc-KanMX 6xGLY-Myc-KanMX
FB
FB
fb
FB2706 DH5a SPO74-GFP pRS314 SPO74-GFP
FB2707 DH5a SPO21-GFP pRS316 SPO21-GFP
FB2702 Jenny Chang pLP891 SIR2 HIS3
FB2703 Jenny Chang 2u pLP1047 SIR3 HIS3
FB2704 Jenny Chang pLP2206 SIR4 HIS3
FB2705 Jenny Chang pPK128 HTA1-HTB1 HHT1-HHF1 LEU2
FB2706 DH5a pRS314 SPO74-GFP
FB2707 DH5a pRS316 SPO21-GFP
FB2708 ApoLife, Nalini Motwani Do not give out. DH5a pM40-3 HPAIX AmpR
FB2709 ApoLife, Nalini Motwani Do not give out DH5a pMC50-2 AmpR
FB2710 Euroscarf for tet regulation DH5a pCM225 AmpR KanR
FB2711 Curt Hagedorn pGEX-4T-3 backbone DH5a p4E-GST AmpR
FB2717 Arndt Lab Derived from pWZ414-F12 (Zhang et al EMBO J. 1998 June1; 17(11):3155-3167. From Shilatifard Histone Mutant Library.

Obtained from Arndt Lab. 		DH5a hht2-K36A-HHF2 hht2-K36A-HHF2 TRP1 CEN3
FB2731 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx313 tRNA HIS3
FB2732 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx313 tRNA/3'ST HIS3
FB2733 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx313 RPR/3'ST HIS3
FB2734 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214. HB101 ampR pIIIEx423 tRNA HIS3
FB2735 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx423 RPR HIS3
FB2736 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx314 tRNA TRP1
FB2737 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx314 RPR TRP1
FB2738 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx424 tRNA TRP1
FB2739 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx424 RPR TRP1
FB2740 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx316 tRNA URA3
FB2741 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx316 tRNA/3'ST URA3
FB2742 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx316 RPR URA3
FB2743 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx316 RPR/3'ST URA3
FB2744 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx426 tRNA URA3
FB2745 David Eugelke (U Michigan) see Gene, 151 (1994) 209-214 HB101 ampR pIIIEx426 RPR URA3
FB2750 WeissmanLab/Stirling Churchman for making ligase for NetSeq. Protein runs around 30 kDA DH5a ampR pT4RNL2(1-249) K227Q
FB2755 Murray Lab pol3 mutator strain
Mol Cell Biol (2005)
vol. 25 (1) pp. 461-71 		PY24D pol3-L523D-URA3
FB2791 Aguilera Lab contains gal inducible RNasH1; from Herrera-Moyano...Aguilera, 2014 pRS416-gal-rnh1 pRS416-gal-rnh1 trp, amp
FB2790 Aguilera Lab contains gal inducible AID; from Garcia-Pichardo...Aguilera et al, 2017, Mol Cel pLZAID pSCH204-gal-aid ura, amp
FB1614 Golderg . good host for expressing foreign proteins in bact. LC137.n... htpR
FB1615 Golberg . Good host for expressing foreign proteins in bact. Clp- Clp-
FB1775 pAB157 w/o HIII msc. fragment ?......... pAB203 LEU2 Amp (H3d.H4)d16'
FB1792 Grant Hartzog derivation:pYEZ417/Bam/HIII into pRS425/Bam/HIII MH1....... pGH186 "Leu2, 2micron, CHD1, ampR
FB1793 Grant Hartzog derivation: pYEZ4-17/Bam/HIII into pRS426/Bam/HIII MH1....... pGH187 "AmpR, URA3, 2micron, CHD1
FB1774 Alex A derivative of pAB148 looking ARS CEN sequences integrate via BstEII in LEU 2 unknown... pAB157 LEU2 Amp
FB1794 Dave Rose . MunI-HIII fragment from p36-1 blunted and cloned into pRS415/SMA1 MH1 pYEZ4-17 "AmpR, LEU2, CEN, ARS, CHD1
FB1795 Dave Rose . genmic clone from Hieter library that contains CHD1 and PAB1 MH1 p36-1 "CEN, ARS, LEU2, AmpR, CHD1, PAB1
FB1801 J. Fassler GalII is Ycp50(LEU2) MH1.alpha pJF765 galII/SPT13 LEU2 CEN Amp
FB1802 M. Ptashne MH1.alpha pHH239 galII TRP1 disruptor
FB1803 D. Stillman DH5-alpha. m1979 "rgr1, LEU2 disruptor
FB1804 D. Stillman DH5-alpha. m2597 "RGR1, URA3, 2micron
FB1805 M. Ptashne see book ?.5-alpha. S023 gal11 CEN LEU2
FB1806 M. Ptashne see book MH1.alpha. gal11wt 2u Myc93 myc-gal11 2 micron LEU2
FB1807 D. Stillman see book MH1.alpha. m1305 Sin+ 2micron URA3
FB1808 D. Stillman MH1.alpha. m1368 Sin4 TRP1 disruptor
FB1809 D. Stillman see book MH1.alpha. m2596 "Rgr1, 2micron, URA3
FB1810 D. Stillman DH5-a.pha. m1977 "rgr1, TRP1 disruptor
FB1811 Audy Hoyt bub2::URA3 ?......... pTR24 ClaI SacI
FB1982 Irina Derkatch DH5a...... pEMBL-D3ATG "CEN, 2micron, SUP35D3ATG
FB1989 .L-1 Blue. HDF1D::TRP1 TRP1 HDF1D
FB2005 Struhl Lab RPD3 null DH5a...... pUC18-Drpd3::HIS3 "RPD3, HIS3
FB2006 Struhl Lab UME6 null DH5a...... pUC19-DUME6::LEU2 "DUME6,LEU2
FB2007 struhl lab gcn5 null DH5a...... YCplac111Dgcn5 "Dgcn5::LEU2,
FB2008 struhl lab RPD3 wild type in YCplac111 DH5a...... RPD3 wt "ADH prom. RPD3, CEN, LEU2
FB2022 MARIAN OSLEY LAB DH5A...... YEPhtb1D3-32 HTA1 "wt HTA1 htb1D3-32, URA3
FB2034 W. Bandlow lab ?.5a...... puc19-leo::URA3 "leo1D::URA3, puc19 bckbone
FB2035 W. Bandlow lab ?.5a...... YEP352-LEO1 LEO1 in YEP352
FB1139 Chris Clark 4.3kb BamHI frag containing SPT16 cloned into BamHI site of YEp24 HB101..... pCC48 SPT16 2u URA3 ampR tetS
FB1140 A. Rowley + Curt Wittenburg HB101.....
FB1234 Nasmyth 2micron LEU2 library in YEp13 (2 vials frozen) unknown... 2micron LEU2 library 2micron LEU2 library
FB71 Carl Falco Yip5+lys2 R1-H3 fragment HB101..... SL42-2 ura3 ampR lys2
FB72 Carl Falco Yip5+lys2 R1-Cla1 fragment HB101..... SL46-1 ura3 ampR lys2
FB26 Dickson Check Fred's notes; this is the b-gal gene from K. lactic DB6507.... pKl6 K. lactis b-gal
FB27 Dickson "see Fred's notes, contains K. lactis b-gal " DB6660.... pKl6 K. lactis b-gal
FB28 Winston DB6507.... pFW10 "tet, URA3, Salmonella hisOGD, ars1
FB29 Johnson and Abelson "SUP6 is an Alu2 fragment with Bam linkers, 750 bp long, tRNAtyr " DB6507.... pYSUP6-Alu2 "amp, SUP6
FB30 Winston HIS4 Sal fragment in YIp5; lacZ Xho fragment in Xho site in HIS4; lacZ fragm ent is from pMC593 DB6507.... pFW11 amp URA3 lacZ HIS4
FB31 Winston "same as pFW11, but opposite lacZ orientation " DB6507.... pFW12 "amp, HIS4, URA3, lacZ
FB34 Winston SUP6 Bam fragment in YEp13 DB6507.... pFW13 LEU2 SUP6 2micron
FB35 Winston "derived from YIp5 and pYeCEN3-41; see Nature 287, 504 " DB6507.... pFW14 ars1 CEN3 URA3 amp
FB36 Winston YIp5 with the HIS4 5' RI fragment; URA3 is inserted in the HIS4 HindIII site DB6507.... pFW7 URA3 HIS4
FB37 Winston same as pFW7 except opposite URA3 orientation; in this plasmid it is in the sameorientation as HIS4 DB6507.... pFW8 URA3 HIS4 amp
FB38 pAJ50 entered elsewhere JM101..... (lac pro)del supE B1/ F' traD36 proAB LacIq lac7M15 pAJ50
FB39 Winston used for 3' experiment DB6507.... pFW15 amp HIS4 241 bp H3-RI fragment
FB40 Winston used for 3' experiment DB6507.... pFW16 amp HIS4 279 bp H3-RI
FB41 Winston Original SPT3 clone candidate 1.6 DB6507.... pFW17 URA3 2 micron SPT3
FB42 Winston Original SPT3 clone 2.37(3-4) DB6507.... pFW18 URA3 SPT3 2 micron amp
FB43 Winston Original SPT3 clone 3.1 DB6507.... pFW19 URA3 SPT3 2 micron amp
FB44 Winston DB6507.... pFW21 YRp10 with SPT3 1.7 Sal fragment
FB45 Winston . DB6507.... pFW20 YRp10 with 1.2 kb RI fragment from SPT3
FB46 Winston This clone has SPT3 function DB6507.... pFW23 pCGS142 with SPT3 H3-BglII fragment
FB47 Winston DB6507.... pFW24 pBR322 with SPT3 H3-BglII fragment
FB48 Winston pFW27= pFW4 with 8kb HindIII frag containing SPT3 DB6507.... pFW27 SPT3 URA3 ampR
FB49 Winston DB6507.... pFW22 pFW19 with 2 small Sal fragments deleted
FB50 Winston DB6507.... pFW28 pFW27 with 1.7 kb Sal fragment deleted
FB51 G. Rubin and A. Spradling E. coli... pPi25.1 pBR322 with SalI fragment from Drosophila containing a P element
FB52 Winston DB6507.... pFW29 SPT3 H3-BglII URA3 amp
FB53 Winston SPT3 fragment in pFW4 DB6507.... pFW30 SPT3 H3-BglII URA3 amp
FB54 Winston SPT3 H3-BglII in pCGS42 DB6507.... pFW31 SPT3 H3-Xho URA3 amp 2 micron
FB55 Winston DB6507.... pFW32 SPT3 RI-BglII URA3 2 micron amp
FB56 Winston kanR from Tn903 on PvuII fragment in pBR PvuII site HB101..... pFW35 amp kan tet
FB57 Winston "same as FB56, but isolate #4, orientations unknown " HB101..... pFW35 amp kan tet
FB58 Winston Contains P element with kanR in it; candidate #10 HB101..... pFW36 pFW36 kan amp
FB59 Winston isolate #12 HB101..... pFW36 same as FB58
FB60 Casadaban via Mike Hall This plasmid in HB101 in strain FB233 M182...... dellacX74 galK galU strA pMC1871 tet lacZ with polylinker
FB61 R. Zitomer. E. coli... YRpBCRC1 galK; protein fusions of CYC1 to galK
FB62 R. Zitomer E. coli... YRpBCR6 "galK, uses galK ATG
FB63 Winston derived from pFW30 DB6507.... pFW33 amp URA spt3-101
FB64 Winston spt3 deletion that goes outside the gene DB6507.... pFW34 amp URA spt3-201
FB65 Winston RI-Xho of SPT3 in RI-Sal of pBR322 DB6507.... pFW42 amp SPT3
FB67 Winston "WRONG, DO NOT USE " DB6507.... pFW44 "WRONG, DO NOT USE
FB66 Winston RI-Xho of SPT3 in YIp5 DB6507.... pFW43 amp URA SPT3
FB69 Winston pFW14 plus HIS4 5' Sal fragment in undetermined orientation DB6507.... pFW38 HIS4 5' amp
FB70 Winston same as pFW38 except has the 912delta Sal fragment DB6507.... pFW39 his4-912delta URA amp
FB74 Winston see FB70 DB6507.... pFW41 same as pFW39 but 2 Sal fragments inserted
FB77 B. Errede HB101..... pAB25 CYC7
FB78 Rose and Winston DB6507.... pMRFW2 ura3-52 amp URA3
FB79 Jensen and Herskowitz DB6507.... YCp50-HO HO URA3 CEN4 ARS1
FB80 J. Abraham via Bik Tye H3 fragment of MATalpho into the H3 site of YEp13 DB6507.... alpha*7.375 MATalpha LEU2 2 micron
FB82 Guarante "Gray et al PNAS 79, 6598; for making lacZ fusions in E. coli " LG90.7.... see FB81 pMR100 lacZ amp
FB83 Winston HIS4 Bgl-Sal (1.2 kb) into pBR322 Bam-Sal DB6507.... pFW45 amp HIS4
FB84 Collaborative 0.85 kb GAL fragment; for PROTEIN fusions DB6507.... pCGS286 GAL UAS 'lacZ URA3 2 micron
FB85 Bik Tye "MboI paritals into Bam site of pCh1. Blue in haploid, white in diploid. " HB101(?).. pCh1-41 "amp, LEU2, lacZ, Ty
FB86 Bik Tye see FB85 HB101?.... pCh1-51 see FB85
FB87 Bik Tye see FB85 HB101?.... pCh1-103 see FB85
FB88 G.S. Roeder DB6507.... R338 amp Ty1-17 URA3
FB89 Winston deletion of Xho fragment in R338 DB6507.... pFW46 amp URA3 1-17delta
FB119 Winston Bgl2-Xho internal LYS2 fragment in pBR322; LYS2 probe HB101..... pFW47 LYS2 amp
FB122 G. Walker pKM101 derivative from Graham Walker JC2926.... recA13 plus many others; look in strain notebook pGW1514 "kanR, pKM101 derivative
FB123 Winston partial evictant of lys2-173R2 HB101..... pFW48 "amp, part of lys2-173R2
FB124 FB124-FB152 all derivatives of pFW32 HB101..... pFW32-302 spt3-302
FB125 HB101..... pFW32-306 spt3-306
FB126 HB101..... pFW32-307 spt3-307
FB127 HB101..... pFW32-309 spt3-309
FB128 . DB6507.... pFW32-318 spt3-318
FB129 . DB6507.... pFW32-319 spt3-319
FB130 . DB6507.... pFW32-322 spt3-322
FB131 Winston DB6507.... pFW32-326 spt3-326
FB132 Winston DB6507.... pFW32-327 spt3-327
FB133 Winston DB6507.... pFW32-328 spt3-328
FB134 Winston DB6507 pFW32-330 spt3-330
FB135 Winston DB6507.... pFW32-332 spt3-332
FB136 Winston DB6507.... pFW32-334 spt3-334
FB137 Winston DB6507.... pFW32-335 spt3-335
FB138 Winston DB6507.... pFW32-336 spt3-336
FB139 Winston DB6507.... pFW32-339 spt3-339
FB140 Winston DB6507.... pFW32-343 spt3-343
FB141 Winston DB6507.... pFW32-343 spt3-343
FB142 DB6507 .B6507.... pFW32-349 spt3-349
FB143 Winston DB6507.... pFW32-354 spt3-354
FB144 Winston DB6507.... pFW32-355 spt3-355
FB145 Winston DB6507.... pFW32-360 spt3-360
FB146 Winston DB6507.... pFW32-364 spt3-364
FB147 Winston DB6507.... pFW32-367 spt3-367
FB148 Winston DB6507.... pFW32-370 spt3-370
FB149 Winston DB6507.... pFW32-374 spt3-374
FB150 Winston DB6507.... pFW32-377 spt3-377
FB151 Winston DB6507.... pFW32-379 spt3-379
FB152 Winston DB6507.... pFW32-382 spt3-382
FB153 Christy DB6660.... trp(am) lac(am) pyrF::Mu r- m+ supF pCC1 SPT3 on RI-XhoI fragment in YCp50
FB154 Winston lys2-173R2 cloned out using pSL42-2 and RI partial HB101..... pFW49 lys2-173R2 URA3
FB155 Winston same as FB154 except a second isolate (b6) HB101..... pFW49 lys2-173R2
FB156 Winston Bam-Sal fragment from lFB128 into Bam-Sal of pBR322. Fragment is internal t o SPT3 and is approx. 0.55 kb long. HB101..... pFW50 internal fragment of SPT3 in pBR322
FB162 Christy mutD generated HB101..... pFW32-218 spt3-218
FB163 Christy mutD generated HB101..... pFW32-219 spt3-219
FB164 Christy mutD generated HB101..... pFW32-220 spt3-220
FB165 Christy mutD generated HB101..... pFW32-221 spt3-221
FB166 Christy mutD generated HB101..... pFW32-224 spt3-224
FB167 Christy mutD generated HB101..... pFW32-226 spt3-226
FB168 Christy mutD generated HB101..... pFW32-228 spt3-228
FB170 Winston deleted Cla fragment from plasmid in strain FB161. HB101..... pFW51 Ty917Cla deletion URA3 amp
FB172 M. Berman single stranded vector KK2186.... pZ152
FB173 M. Berman KK2186.... none
FB175 Winston made by eviction using YIp6 (alias YIp301?) HB101..... pFW52 Ty917(480) URA3 amp
FB176 Fields and Herskowitz This contains an alpha factor-lacZ fusion in YCp50 ?.101..... YCp50::fz46 alpha factor lacZ URA
FB177 Christy HB101..... pFW32-232 spt3-232
FB178 Christy HB101..... spt3-233
FB179 Christy HB101..... spt3-240
FB180 Christy .B101..... spt3-242
FB181 Chirsty HB101..... spt3-242
FB182 Christy HB101..... spt3-243
FB183 Christy HB101..... spt3-244
FB184 Christy HB101..... spt3-245
FB185 Christy HB101..... spt3-246
FB186 deleted .B101.....
FB187 Christy HB101..... spt3-248
FB188 Christy HB101..... spt3-249
FB189 Christy .B101..... spt3-250
FB190 Christy HB101..... spt3-251
FB191 Christy HB101..... spt3-252
FB192 Christy HB101..... spt3-253
FB193 Christy .B101..... spt3-254
FB194 Christy HB101..... spt3-255
FB195 Christy HB101..... spt3-256
FB196 Christy HB101..... spt3-257
FB197 Christy . HB101..... spt3-258
FB198 Christy HB101..... spt3-259
FB199 Christy HB101..... spt3-260
FB200 Christy HB101..... spt3-261
FB201 Christy HB101..... pFW32-262 spt3-262
FB202 Christy HB101..... pFW32-263 spt3-263
FB203 Christy HB101..... pFW32-264 spt3-264
FB204 Christy .B101..... pFW32-265 spt3-265
FB205 Christy HB101..... pFW32-266 spt3-266
FB206 Christy .B101..... pFW32-267 spt3-267
FB207 Christy HB101..... pFW32-268 spt3-268
FB208 Christy HB101..... pFW32-269 spt3-269
FB209 Christy HB101..... pFW32-270 spt3-270
FB210 Christy .B101..... pFW32-271 spt3-271
FB211 Christy HB101..... pFW32-272 spt3-272
FB212 Christy HB101..... pFW32-273 spt3-273
FB213 Christy HB101..... pFW32-274 spt3-274
FB214 Christy .B101..... pFW32-275 spt3-275
FB215 Christy HB101..... pFW32-276 spt3-276
FB216 Christy HB101..... pFW32-277 spt3-277
FB217 Christy HB101..... pFW32-278 spt3-278
FB218 Christy HB101..... pFW32-279 spt3-279
FB219 Christy HB101..... pFW32-280 spt3-280
FB220 Christy HB101..... pFW32-281 spt3-281
FB221 Christy HB101..... pFW32-282 spt3-282
FB222 Christy HB101..... pFW32-283 spt3-283
FB223 Christy HB101..... pFW32-284 spt3-284
FB224 Christy HB101..... pFW32-285 spt3-285
FB227 "MCB 4, 657-665, Maynard Olsen UGA suppressor .......... pPM600 SUP4-UGA CEN3 ampR TRP1 ARS1
FB228 Patty and Fred high copy number HB101..... pFW53 spt3::lacZ amp URA3
FB229 Patty and Fred Deletion of the HpaI fragment from Ty917 from FB161 plasmid HB101..... pFW54 amp URA3 Ty917-HpaI deletion
FB230 Patty and Fred Deletion of HpaI from Ty917(480) HB101..... pFW55 amp URA3 Ty917(480)HpaI deletion
FB233 M. Casadaban Plasmid originally in B60; put in HB101 for better plasmid preps HB101..... pMC1871 tet 'lacZ
FB234 Patty and Fred HB101..... pFW56 amp spt3::lacZ
FB235 Christy subclone of LYS2 RI-H3 into YCp50 DB6507.... pCC2 amp URA3 LYS2 CEN
FB238 "J. Gorman, SmithKline for promoter fusions to galK E. coli... pYSK7-54 amp TRP1 'galK CEN3
FB239 "J. Gorman, SmithKline for protein fusions to galK .. coli... pYSK9-8 amp TRP1 galK CEN3
FB240 "J. Gorman, SmithKline for protein fusions to galK .. coli... pYSK9-10 amp TRP1 'galK CEN3
FB241 "J. Gorman, SmithKline for protein fusions to galK .. coli... pYSK9-12 amp TRP1 'galK CEN3
FB242 Patty and Fred out of frame fusion SPT3-lacZ RR1delM15. pFW57 lacZ amp spt3'
FB243 Patty and Frecd out of frame SPT3-lacZ fusion RR1delM15. pFW58 lacZ amp spt3'
FB244 Patty and Fred "In frame fusion, SPT3-lacZ in pUR292 " RR1delM15. pFW59 amp SPT3-lacZ
FB245 Patty and Fred "fusion to Bam linker at spt3-302, approx. 40 aa of SPT3 included " HB101.M15. pFW60 amp URA3 SPT3-lacZ
FB246 Patty and Fred SPT3-lacZ with lacZ in wrong orientation HB101.M15. pFW61 amp URA3 'lacZ
FB247 Patty and Fred SPT3 fusion at spt3-382; wrong orientation HB101.M15. pFW62 URA3 amp 'lacZ
FB248 Patty and Fred fusion at spt3-382; lacZ in twice HB101.M15. pFW63 amp URA3 'lacZ SPT3'
FB249 Fred deletion between spt3-302 and spt3-382 RR1delM15. pFW64 URA3 amp spt3del
FB250 Fred insert of 'lacZ into BglII of pSL42-2 RR1delM15. pFW65 amp URA3 lys2::lacZ
FB251 Patty and Fred URA3 in HindIII site of Ty917(480) clone (pFW52) HB101..... pFW66 amp Ty917(480)-URA3 URA3
FB252 Patty and Fred subclone 'lacZ into spt3-382 HB101..... pFW67 amp spt3-382::lacZ 2 micron
FB253 . "EMBO 2, 1971-1794 (1983); for lacZ fusions for antibodies " RR1delM15. lacZdel r- pUR290 ampR lacZ
FB254 "EMBO 2, 1791-1794 (1983) for lacZ fusions for antibodies RR1delM15. lacZdel r- pUR291 lacZ amp
FB255 "EMBO 2, 1971-1794 (1983) for lacZ fusions for antibodies RR1delM15. lacZdel r- pUR292 amp lacZ
FB256 out of frame HB101.M15. pFW68 amp URA3 lys2-128delta::lacZ
FB257 Fred spt3-202 in YIp5 HB101.M15. pFW69 amp URA3 spt3-202
FB258 RI 1.4 kb fragment contains TRP1 and ARS1 into RI site of pBR322 .B101.M15. YRp7 TRP1 ars1 tet amp
FB259 Rose and Botstein can be used for fusions .B101.M15. pRB45 LEU2 URA3 amp 2 micron 'lacZ
FB260 RI-Xho of SPT3 RR1delM15. pFW42 amp SPT3
FB261 Fred Fusion of Ty917 to lacZ HB101..... pFW70 amp URA3 Ty917-lacZ
FB262 Fred "Ty917-lacZ, same as pFW70; different isolate " HB101..... pFW71 amp URA3 Ty917-lacZ
FB263 Fred "Ty917-lacZ fusion; same as pFW70, 71 but different isolate " HB101..... pFW72 amp URA3 Ty917-lacZ
FB264 Fred GAL-SPT3 fusion HB101..... pFW73 GAL-SPT3 URA3 2 micron
FB265 Fred "GAL-SPT3 fusion, fused at spt3-354 (different from pFW73) " HB101..... pFW74 amp URA3 SPT3 GAL-SPT3
FB266 Fred GAL-SPT3 fusion at spt3-374 HB101..... pFW75 amp URA3 GAL-SPT3 2 micron
FB267 Fred SPT3 is in the wrong orientation!!! HB101..... pFW76 amp URA3 GAL-SPT3 2 micron
FB268 BRL a useful plasmid JM83...... ara del(lac-pro) thi strA phi80dlacZ delM15 pUC18 amp
FB269 BRL Another useful plasmid JM83...... ara del(lac-pro) thi strA phi80dlacZ delM15 pUC19 amp
FB270 "Fred, Patty, and Christy spt3-244 is an ochre mutation HB101..... pFW77 amp URA3 spt3-244
FB271 "Fred, Patty, and Christy spt3-269(UGA) HB101..... pFW78 amp URA3 spt3-269(UGA)
FB272 "Fred, Christy, Patty spt3-271(UAA) HB101..... pFW79 amp URA3 spt3-271(UAA)
FB273 ? maybe Ben Hall lab HindIII fragment of PYK1 in pBR322 HB101..... pFR2 PYK1 amp
FB275 Clare and Farabaugh Ty-lacZ fusion HB101..... pJCdel44 "amp, 2 micron, Ty-lacZ
FB276 Clare and Farabaugh Ty-lacZ fusion HB101..... pJCdel35 amp 2 micron Ty-lacZ
FB277 Clare and Farabaugh Ty-lacZ fusion HB101..... pJCdel38 amp 2 micron URA3 Ty-lacZ fusion
FB278 Clare and Farabaugh Ty-lacZ fusion HB101..... pJCdel36 amp URA3 2 micron Ty-lacZ
FB279 Clare and Farabaugh Ty-lacZ fusion HB101..... pJCdel37 amp URA3 2 micron Ty-lacZ fusion
FB280 FW FB274..... pFW79 amp URA3 spt3-271(UAA)
FB284 R. Surosky Beware of this plasmid. May be incorrect. HB101..... his4-912delta
FB286 Heffron see notebook RDP146.... pOX38::m-Tn3(lacZ)
FB287 Heffron see notebook RDP146.... pOX38::m-Tn3(URA3)
FB288 Heffron see notebook RDP146.... pOX38::m-Tn3(TRP1)
FB289 Heffron see notebook RDP146.... pOX38::m-Tn3(HIS3)
FB290 Heffron see notebook RDP146.... pOX38::m-Tn3(LEU2)
FB291 Heffron see notebook RDP146.... pOX38::m-Tn3
FB292 Heffron see notebook RDP146.... pOX38::M-Tn3leu2-lac
FB293 Heffron see notebook RDP146.... pOX38::m-Tn3his3-lac
FB294 Heffron see notebook RDP146.... pOX38::m-Tn3ura3-lac
FB295 Heffron see notebook RDP146.... pOX38::m-Tn3trp1-lac
FB296 Heffron see notebook RDP146.... pLB101
FB297 Heffron see notebook RDP145.... pHSS6
FB298 Heffron see notebook NS2114....
FB299 Kate isolatae 1.2 FB285..... pFW80 pUC18 912delta Rsa-Taq
FB300 Kate . isolate 1.3 FB285..... pFW80 pUC18 912 delta Rsa-Taq
FB301 Kate isolate 1.4 FB285..... pFW80 pUC18 912delta Rsa-Taq
FB302 Fred lacZ in spt3-334 HB101..... pFW81 amp URA3 spt3::lacZ
FB303 Kate "from pJCdel44, integrating version " FB1.1..... pFW82 amp ura3 Ty-lacZ
FB304 Kate "from pJCdel44, integrating version " FB1.1..... pFW83 amp ura3 Ty-lacZ
FB305 Kate Ty from pJCdel35; integrating version FB1.1..... pFW84 amp ura3 Ty-lacZ
FB306 Kate from pJCdel35; integrating version FB1.1..... pFW85 amp ura Ty-lacZ
FB307 Kate from pJCdel37; integrating version FB1.1..... pFW86 amp ura3 Ty-lacZ
FB308 Kate from pJCdel37; integrating version FB1.1..... pFW87 amp ura3 Ty-lacZ
FB309 Promega see notebook HB101..... pGEM1
FB310 Promega see notebook HB101..... pGEM2
FB315 L. Guarente "Guarente and Ptashne, Cell (1980); for fusions " HB101.n... pLGdel312 amp URA3 'lacIZ 2 micron
fb312 Mark Rose KAR1 probe; see notebook HB101.n... pMR95 KAR1 ampR
FB313 Susan Michaelis MFa2 probe; published in MCB HB101.n... pSM29 MFa2 ampR
FB314 Susan Michaelis MFa1 probe; published in MCB HB101.n... pSM39 MFa1 ampR
FB316 L. Guarente "Guarente et al., Cell, Feb. 1984; CEN vector for fusions " HB101.n... pBL101 ampR URA3 'lacIZ CEN4 ARS1
FB317 D. Garfinkel high copy number suppressor HB101.n... CS14 ampR; high copy SPT23
FB319 D. Botstein "see Ng and Abelson, PNAS 77,3912 (1980) " ..101.n... pRB149 amp tet URA3
FB320 Jodi Hirschman For making SPT3 in vitro; SPT3 Sma-EcoRI under SP6 control HB101.n... amp SP6-SPT3 pJEH1 SPT3 transcription under SP6 control in pGEM1
FB321 Jodi spt3-101 under SP6 control HB101..... pJEH2 amp SP6-spt3-101
FB322 Jodi SPT3 under T7 control HB101..... pJEH3 amp T7-SPT3
FB323 Jodi spt3-101 under T7 control HB101..... pJEH4 amp T7-spt3-101
FB324 Jodi His+ derivative of YCp701 HB101..... YCp701-R5 amp his4-912dR5 URA3
FB325 Jodi His+ derivative of YCp701 HB101..... YCP701-R58 amp URA his4-912dR58
FB329 Rick Gaber Replaced tet region of YIp5 with pUC18 polylinker HB101..... pRG331 amp URA3 pUC polylinker
FB330 Rick Gaber "WARNING!!- At low frequency, the plasmid loops out between the ori and polyl inker. tet region of YCp50 replaced with pUC18 polylinker " HB101..... pRG332 amp URA3 CEN4 ARS1 pUC18 polylinker
FB331 Jodi His+ revertant of his4-912d HB101..... YCp701-R177 URA CEN4 ARS1 his4-912dR177
FB333 T.J. Koerner and Tzagaloff see notebook; for fusions HB101..... pATH1 amp trpE
FB334 T.J. Koerner and Tzagaloff see notebook; for fusions HB101..... pATH2 amp trpE
FB335 T.J. Koerner and Tzagaloff see notebook; for fusions HB101..... pATH3 amp trpE
FB336 Kate and Fred in pATH1; fusion at spt3-302 BamHI linker MC1061.... pFW88 URA3 trpE-SPT3
FB337 Kate and Fred fusion at spt3-302 in pATH1 MC1061.... pFW89 amp trpE-SPT3
FB338 Kate and Fred fusion in pATH2; at spt3-302 MC1061.... pFW90 amp trpE-SPT3
FB339 Kate and Fred fusion in pATH2 at spt3-302 MC1061.... pFW91 amp trpE-SPT3
FB340 Kate original clone candidate HB101..... pFW92 amp URA CEN SPT8
FB341 Kate original SPT8 clone candidate HB101..... pFW93 amp URA3 CEN4 ARS1 SPT8
FB342 Kate original SPT8 clone candidate HB101..... pFW94 amp URA3 CEN4 ARS1 SPT8
FB343 Kate original SPT8 clone candidate HB101..... pFW95 amp URA3 CEN4 ARS1 SPT8
FB344 Kate original SPT8 clone candidate HB101..... pFW96 amp URA3 CEN4 ARS1 SPT8
FB345 Kate pATH1 vector; in frame trpE-SPT3 fusion RR1....... pFW88 trpE-SPT3 amp
FB346 Kate "pATH1 vector, trpE-SPT3 in frame fusion " RR1....... pFW89 trpE-SPT3
FB347 Kate fusion in pATH2 RR1....... pFW90 amp trpE-SPT3 out of frame
FB348 Kate in pATH2 RR1....... pFW91 amp trpE-SPT3 out of frame
FB349 Fred EcoRV-BglII of lys2-128d in pUC18 TB1....... pFW97 amp lys2-128d 5' end
FB350 Fred EcoRV-BglII of lys2-128d in pUC19 TB1....... pFW98 amp lys2-128d 5' end
FB353 D. Botstein SUC2 probe HB101..... pRB59 amp SUC2 internal fragment
FB354 Kate original SPT8 clone HB101..... pFW99 YCp50 SPT8
FB355 Kate original SPT8 clone HB101..... pFW100 YCp50 SPT8
FB356 Kate original SPT8 clone HB101..... pFW101 YCp50 SPT8
FB357 Kate original SPT8 clone HB101..... pFW102 YCp50 SPT8
FB358 Kate original SPT8 clone HB101..... pFW103 YCp50 SPT8
FB359 Kate original clone HB101..... pFW104 YCp50 SPT7
FB360 Kate original clone HB101..... pFW105 YCp50 SPT7
FB361 Kate original clone HB101..... pFW106 YCp50 SPT7
FB362 Kate original clone HB101..... pFW107 YCp50 SPT7
FB363 Kate original clone HB101..... pFW108 YCp50 SPT7
FB364 Kate original clone HB101..... pFW109 YCp50 SPT7
FB365 Kate original clone HB101..... pFW110 YCp50 SPT7
FB366 Rick Gaber same as in FB330 with unknown difference HB101..... pRG377 YCp50 pUC18 polylinker
FB866 Anne Chiang probably non-functional; should be rechecked TB1.1..... pAC20 pRS316 SPT9 region Bam fragment
FB867 Anne Chiang not tested for function .B1.1..... pAC22 "SPT9, SacI piece reclosed
FB868 Christine see notebook HB101..... pSPT8del15 spt8 acidic deletion 1.5 using oligo D1
FB869 Christine deletion of amino acids 39-76 using oligo D7 HB101..... pSPT8del6.20 spt8 acidic deletion 6.20
FB870 Dave 2.2 kb Sal-Xho LEU2 into Sal site in polylinker of pSE360 TB1.1..... pDE48-1 LEU2 CEN ARS URA3
FB871 Dave see notebook TB1.1..... pDE51-5 spt15::LEU2 YCp50
FB872 Dave TB1....... pDE53-1 pRS306 spt15-122
FB873 Dave TB1....... pDE54-1 pRS306 spt15-135
FB874 Dave y TB1....... pDE55-1 pCGS42 spt15-21
FB875 Dave TB1....... pDE56-1 pRS306 spt15-21
FB876 Dave "pSE358 is CEN ARS TRP plasmid; reference is Gene 70, 303-312 " TB1....... pDE58-1 spt15-21 pSE358
FB877 Dave For gap rescue of spt15 alleles; digest with RI; use largest fragment; see F B900 for URA3 version TB1....... pDE59-1 pRS314 SPT15 6.6kb Cla-Sac fragment
FB878 Dave TB1....... pDE67-3 pCGS42 spt15-122
FB879 Dave "pSE358 reference is Gene 70, 303-312 " TB1....... pDE68-4 pSE358 (CEN TRP ARS) spt15-122
FB880 Dave Both SPT15 and TRP1 are inserted in this plasmid; see notebook TB1....... pDE71-11 pRS316 SPT15 TRP1
FB881 Dave Sal site put in by Karen; see notebook TB1....... pDE73-21 pRS316 SPT15 with Sal site at 18 bp upstream of ATG
FB882 Dave TB1....... pDE74-6 pRS316 spt15-26
FB883 Dave see notebook; Sal site 18 bp upstream of ATG TB1....... pDE75-2 pRS314 SPT15-Sal
FB884 Dave Sal site 18 bp upstream of ATG TB1....... pDE76-4 pCGS42 SPT15-Sal
FB885 F.W. Studier FB885-894 are for expression via T7 RNA polymerse. See Fred or Greg for the article describing all of these vectors and strains. BL21 (DE3) pLysS
FB886 F.W. Studier see FB885 HB21 (DE3) pLysE
FB887 F.W. Studier see FB885 BL21.(DE3) none
FB888 F.W. Studier see FB885 BL21 (DE3) none
FB889 F.W. Studier see FB885 HMS174.E3) none
FB890 F.W. Studier see FB885 HMS174.E3) pET3
FB891 F.W. Studier see FB885 HMS174.E3) pET-39
FB892 F.W. Studier see FB885 HMS174.E3) pET-36
FB893 F.W. Studier see FB885 HMS174.E3) pET-8c
FB894 F.W. Studier see FB885 HMS174.E3) pET-3c
FB895 Bob Moreland "see Cell 45, 537-544 " HB101..E3) pSKAdel8-7 HTA1-lacZ fusion
FB896 Kate see notebook TB1.1..E3) pFW253 HTB2-lacZ fusion
FB897 Kate TB1.1..E3) pFW254 pRS314 SPT7 Bam-RI
FB898 Joel Bases 1-228 by Genbank TB1.1..E3) pJH11 pUC18 HIS4 Sal-Xho 228 bp fragment
FB899 Joel Opposite orientation as in FB898 TB1.1..E3) pJH12 pUC18 HIS4 Sal-Xho 228 bp fragment
FB900 Dave for rescue of spt15 alleles; cut with RI; see FB877 for TRP1 version HB101..... pDE38-9 pRS316 SPT15 6.6 kb Cla-Sac
FB901 Kate TB1....... pFW255 pRS314 SPT7 Cla-RI
FB902 Christy "probe for HTA1, HTB1; also includes protein 1 " HB101..... pCC68 pBR322 HTA1-HTB1 5.5kb NheI-SalI
FB903 Kate HB101..... pFW256 YCp50 HTA2-HTB2
FB904 Kate HB101..... pFW257 pCGS42 HTA2-HTB2
FB905 Suzanne Sandmeyer "Ty3 probe; MCB 8, 5245 " HB101..... pTy3-2 "amp, F1 ori, pBR322 ori, T7 promoter, Ty3-2
FB906 Maynard Olsen "Gene 27, 239 " unknown... pPM256 "2 micron, LEU2, SUP53-a
FB907 Maynard Olsen note: SUP4-a Bam fragment is 235 bp. See Molecular Cell Biology 4 (1984) p. 657-665 unknown... pPM609 SUP4-a TRP1 ARS1 CEN3 ampR
fb908 M.A. Osley .97 kb H3 promoter of TRT2 cloned into YIp5 HB101.n... Yip5-2.97M TRT2 promoter URA3 ampR
FB909 Michele Swanson/ Fred Winston 1.5kb Bam-Sal fragment from pAD5 cloned into Bam-Sal sites of 8.0kb YEp353 TB1 ..n... pMS35 ampR ura3 2micron
FB910 Tricia Davis .B1 ..n...
FB911 Tricia Davis AB1886 F- uvrA6 thr1 leu6 thi1 lacV1 galkz ara14 xyl5
FB912 Tricia Davis SEE FB911 and enclosed information in notebook AB1886.... pGW249 pKM10bla::Tri5 kanR
Fb913 Michele Swanson/ Fred Winston =6kb BglII fragment enclosing SPT6 at BamHI site of YCp50(pCC11) with insert ion of HAI epitope sequences between first and second codon of SPT6 HB101..... pMS33 ampR URA3 CEN ARS SPT6
FB914 "K.Ferguson.n, Wigler lab see notebook HB101 pAD5 Lerner Epitope
FB915 Christine Chapon SPT8 Bam-Bam in YCp50; HAS NO FUNCTION unknown... pY2 SPT8
FB916 Christine Chapon SPT8 Bam-Bam in YCp50; HAS NO FUNCTION; is a duplicate of FB915 unknown... pY3 SPT8
FB917 Christine Chapon SPT8 Bam-Bam in YCp50; opposite orientation as FB916; has partial function unknown... pY7 SPT8
FB918 Christine Chapon SPT8 Bam-Bam in YCp50; opposite orientation as FB916; has partial function; is a duplicate of pY7 in FB917 unknown... pY8 SPT8
FB919 Christine Chapon SPT8 Bam-Bam in pBM272 unknown... p7.4(757-4) SPT8
FB920 Christine Chapon "SPT8 Bam-Bam in pBM272, same as p7.4 in FB919 " unknown... p7.14(757-14) SPT8
FB921 Christine Chapon "SPT8 Bam-Bam in pBM272, opposite orientation from p7.4 in FB919 " unknown... p7.5(757-5) SPT8
FB922 Christine Chapon "SPT8 Bam-Bam in pBM272, same as p7.5 in FB921 " unknown... p7.15(757-15) SPT8
FB923 Dave Deitcher TG1.own... "JM101, Ecok
FB942 Greg Prelich TB1.1..... pGP43 ampR LEU2
FB943 Greg Prelich TB1.1..... pGP44 ampR URA3
FB944 Greg Prelich TB1.1..... pGP45 ampR TRP1
FB945 Dan Finley HB101..... pUB200 UBI4 ampR
FB946 Dan Finley Make DNA from this strain if you want to cut at BclI site (enzyme is sensiti ve to methylation). FB432..... dam- pUB200 UBI4 ampR
FB947 Steve Buratowski/Sharp Lab "Ref: Cell 56, p. 549-561 " HB101..... pRW ampR
FB948 S. Ricupero "~1kb BglII to ClaI fragment of RSP4+ rescued from yeast, was cloned into puc 19 BamHI to AccI. All four restriction sites have been destroyed. " HB101..... pFW258 ampR ori lacZ
FB949 Sikorski/Hieter See sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS403 HIS3 ampR
FB950 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS404 TRP1 ampR
FB951 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS405 LEU2 ampR
FB952 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS406 URA3 ampR
FB953 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS413 CEN ARS HIS3 ampR
FB954 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS414 CEN ARS TRP1 ampR
FB955 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS415 CEN ARS LEU2 ampR
FB956 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS416 CEN ARS URA3 ampR
FB957 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha pRS423 2micron HIS3 ampR
FB958 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS424 2micron TRP1 ampR
FB959 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS425 2micron LEU2 ampR
FB960 Sikorski/Hieter SEE FB949 and sheets in notebook! 1990 Cold Spring Harbor DH5alpha.. pRS426 2micron URA3 ampR
FB961 Sikorski/Hieter For chromosome fragmentation. 1990 Cold Spring Harbor DH5alpha.. pYCF3 p299 URA3 SUP11 (see FB522)
FB962 Sikorski/Hieter For chromosome fragmentation. 1990 Cold Spring Harbor DH5alpha.. pYCF4 p300 URA3 SUP11 (see FB523)
FB1440 .B1.1..... pT2
FB1441 .B1.1..... pT1-3B
FB1442 .B1.1..... pIP5469
FB2082 Berger Lab DH5a...... A/C GV "gal4-VP16 Leu2, CEN
FB2083 Berger Lab F to A mutant DH5a...... A/C FA gal4-VP16 LEU2 CEN
FB2084 Berger Lab DH5a...... G/V "2micron Gal4-VP16, LEU2
FB2181 David Stillman "resistant to killing by DNA gyrase, for use with pRS416-0 " NH975.....
FB2182 Beckwith Lab DHB4......
FB2293 Danesh Moazed DH5a...... pRS315-SIR3 "CEN, LEU2, SIR3
FB2701 Mei Sheng/Niroshi Senaratne Generated by Mei Sheng during her rotation by inserting URA3 into LacZ in the pRS313 Myc-SPN1 HIS3 CEN AmpR plasmid received by Erin Loeliger from the Stargell Lab. This plasmid containing URA3 was extracted from yeast by Niroshi Senaratne and transformed into E. coli, here stored as FB2701. DH5a HIS3 URA3 Myc-SPN1 AmpR CEN
FB2356 Natalie Kuldell XL1....... pRS415-TFG2 "LEU2, CEN, TFG2
FB2357 Natalie XL1....... pRS416-TFG2 "URA3, CEN, TFG2
xit
rothstein
pGH127
pGH%
FB
pFW217
pRM200
pRM%
ura3 his3
snf1
ura3
%FLAG%
Joel
hml
%hml%
pNOY436
FB2496 Carl Wu H2B1 in supercodon plus strain BL21 pET28b-H2B1 H2B.1
FB2497 Carl Wu H3.2 supercodon plus strain BL21 pET28b-H3.2 H3.2
FB2498 Carl Wu H4.2 supercodon plus strain BL21 pET28b-H4.2 H4.2
fb2195
phz1
pms4
FB374 Kate Cla-Sal subclone MC1061.... pFW111 amp SPT7 YCp50
FB375 Fred LYS2 5' probe MC1061.... pFW112 amp LYS2 RI-BglII 5' fragment
FB376 F. del Rey and G.R. Fink RsaI fragment of sigma plasmid into ClaI of YIp5 .C1061.... pML52 URA3 sigma
FB377 Scott Emr vector for lacZ fusions MC1061.... pSEY101 ura3 lacZ 2 micron amp
FB378 Scott Emr CEN vector for lacZ fusions MC1061.... pSEYC102 ura3 ars1 cen4 amp lacZ
FB379 Scott Emr for lacZ fusions FB383..... pSEY8 amp 2 micron lacZ
FB380 Scott Emr for lacZ fusions FB383..... pSEYC58 amp cen4 ars1 lacZ
FB382 Scott Emr for lacZ fusions FB383..... pSEYC68 cen4 ars1 ura3 lacZ amp
FB384 Kate partial SPT7 activity MC1061.... pFW116 amp ura SPT7 ClaI-SalI
FB385 Kate no SPT7 activity MC1061.... pFW117 YCp50 SPT7 SphI-RI from pFW110
FB386 Kate no SPT7 activity MC1061.... pFW118 YIp5 SPT7 SphI-RI from pFW110
FB387 Kate no SPT7 activity MC1061.... pFW119 YCp50 SPT7 SphI-Ri
FB388 Kate has SPT8 activity MC1061.... pFW120 YCp50 SPT8 ClaI-SphI
FB389 Kate has SPT8 activity MC1061.... pFW121 YIp5 SPT8 ClaI-SphI
FB390 Kate 2 kb SPT7 fragment MC1061.... pFW122 pBR322 SPT7 Cla-Sal
FB391 Kate see FB381 HB101..... pSEY18 lacZ amp ura3 2 micron
FB392 Scott Emr see FB382 HB101..... pSEYC68 cen4 ars1 ura3 lacZ amp
FB393 Kate 3 kb fragment of SPT7 MC1061.... pFW123 YCp50 SPT7 Sal-RI
FB394 Kate MC1061.... pFW124 YIp5 SPT7 Sal-RI
FB395 Kate y MC1061.... pFW125 YCp50 SPT7 Sal fragment
FB396 Jef Boeke subclone from P2-L4 into Cla site of pBR325 FB1.61.... pJEF705 LYS2 pBR325

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