Director of Biology at Addgene
Research in our lab has led to models explaining how mutant yellow alleles complement each other when they are paired. In the transcription-based model, enhancers can act in trans on the promoter of a paired homolog when the cis promoter is non-functional. In the structure-based model, pairing of structurally different alleles leads to looping out of non-homologous regions of DNA, bringing enhancers closer to their promoter. In both models, homolog pairing must precede transvection.
My research addresses pairing. My questions
are:
1. What are the requirements for pairing at the
yellow locus?
2. What are the requirements for pairing between
yellow transgenes?
3. Do chromosomes in yeast pair and allow for
transvection?