In June, 2006, we published a method for Drosophila transgenesis that targets constructs to predetermined genomic sites using the phiC31 integrase system in conjunction with Recombinase Mediated Cassette Exchange (RMCE). This website is intended as a resource for researchers who wish to use phiC31-mediated RMCE in their studies. The site is a work in progress - we hope you find it useful, and we welcome feedback.
We have moved updates of new data, reagents, and input from others to our news/notes page. In addition, the navigation bar to the left provides links to an overview of the technique, sequence information for the vectors we designed, information on existing genomic target insertions, and some useful protocols.
Features:- Site-specific integration
- No integration of vector sequences
- No requirement to integrate selectable marker (negative
selection)
- Efficiency comparable to P-element transgenesis
- Allows subsequent rounds of integration in the presence of a previous
integrant
- Allows subsequent use of other site-specific recombinases such as Cre
and FLP
- Screening against genomic marker prevents issues with integration at
non-target pseudo-attP sites